TY - JOUR A1 - Rohn, Isabelle A1 - Kroepfl, Nina A1 - Bornhorst, Julia A1 - Kühnelt, Doris A1 - Schwerdtle, Tanja T1 - Side-directed transfer and presystemic metabolism of selenoneine in a human intestinal barrier model JF - Molecular nutrition & food research : bioactivity, chemistry, immunology, microbiology, safety, technology N2 - Scope: Selenoneine, a recently discovered selenium (Se) species mainly present in marine fish, is the Se analogue of ergothioneine, a sulfur-containing purported antioxidant. Although similar properties have been proposed for selenoneine, data on its relevance to human health are yet scarce. Here, the transfer and presystemic metabolism of selenoneine in an in vitro model of the human intestinal barrier are investigated. Methods and results: Selenoneine and the reference species Se-methylselenocysteine (MeSeCys) and selenite are applied to the Caco-2 intestinal barrier model. Selenoneine is transferred in higher amounts, but with similar kinetics as selenite, while MeSeCys shows the highest permeability. In contrast to the reference species, transfer of selenoneine is directed toward the blood side. Cellular Se contents demonstrate that selenoneine is efficiently taken up by Caco-2 cells. Moreover, HPLC/MS-based Se speciation studies reveal a partial metabolism to Se-methylselenoneine, a metabolite previously detected in human blood and urine. Conclusions: Selenoneine is likely to pass the intestinal barrier via transcellular, carrier-mediated transport, is highly bioavailable to Caco-2 cells and undergoes metabolic transformations. Therefore, further studies are needed to elucidate its possible health effects and to characterize the metabolism of selenoneine in humans. KW - bioavailability KW - Caco-2 intestinal barrier model KW - presystemic metabolism KW - selenoneine KW - Se-methylselenoneine Y1 - 2019 U6 - https://doi.org/10.1002/mnfr.201900080 SN - 1613-4125 SN - 1613-4133 VL - 63 IS - 12 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Meyer, Sören A1 - Raber, Georg A1 - Ebert, Franziska A1 - Taleshi, Mojtaba S. A1 - Francesconi, Kevin A. A1 - Schwerdtle, Tanja T1 - Arsenic-containing hydrocarbons and arsenic-containing fatty acids: Transfer across and presystemic metabolism in the Caco-2 intestinal barrier model JF - Molecular nutrition & food research : bioactivity, chemistry, immunology, microbiology, safety, technology N2 - Scope: Arsenic-containing hydrocarbons (AsHCs) and arsenic-containing fatty acids (AsFAs) represent two classes of arsenolipids occurring naturally in marine food. Toxicological data are yet scarce and an assessment regarding the risk to human health has not been possible. Here, we investigated the transfer and presystemic metabolism of five arsenolipids in an intestinal barrier model. Methods and results: Three AsHCs and two AsFAs were applied to the Caco-2 intestinal barrier model. Thereby, the short-chain AsHCs reached up to 50% permeability. Transport is likely to occur via passive diffusion. The AsFAs showed lower intestinal bioavailability, but respective permeabilities were still two to five times higher as compared to arsenobetaine or arsenosugars. Interestingly, AsFAs were effectively biotransformed while passing the in vitro intestinal barrier, whereas AsHCs were transported to the blood-facing compartment essentially unchanged. Conclusion: AsFAs can be presystemically metabolised and the amount of transferred arsenic is lower than that for AsHCs. In contrast, AsHCs are likely to be highly intestinally bioavailable to humans. Since AsHCs exert strong toxicity in vitro and in vivo, toxicity studies with experimental animals as well as a human exposure assessment are needed to assess the risk to human health related to the presence of AsHCs in seafood. KW - Arsenolipids KW - Caco-2 intestinal barrier model KW - Presystemic metabolism KW - Toxicity Y1 - 2015 U6 - https://doi.org/10.1002/mnfr.201500286 SN - 1613-4125 SN - 1613-4133 VL - 59 IS - 10 SP - 2044 EP - 2056 PB - Wiley-Blackwell CY - Hoboken ER -