TY  - JOUR
A1  - Luetkecosmann, Steffi
A1  - Faupel, Thomas
A1  - Porstmann, Silvia
A1  - Porstmann, Tomas
A1  - Micheel, Burkhard
A1  - Hanack, Katja
T1  - A cross-reactive monoclonal antibody as universal detection antibody in autoantibody diagnostic assays
JF  - Clinica chimica acta
N2  - Diagnostics of Autoimmune Diseases involve screening of patient samples for containing autoantibodies against various antigens. To ensure quality of diagnostic assays a calibrator is needed in each assay system. Different calibrators as recombinant human monoclonal antibodies as well as chimeric antibodies against the autoantigens of interest are described. A less cost-intensive and also more representative possibility covering different targets on the antigens is the utilization of polyclonal sera from other species. Nevertheless, the detection of human autoantibodies as well as the calibration reagent containing antibodies from other species in one assay constitutes a challenge in terms of assay calibration. We therefore developed a cross-reactive monoclonal antibody which binds human as well as rabbit sera with similar affinities in the nanomolar range. We tested our monoclonal antibody S38CD11B12 successfully in the commercial Serazym (R) Anti-Cardiolipin-beta 2-GPI IgG/IgM assay and could thereby prove the eligibility of S38CD11B12 as detection antibody in autoimmune diagnostic assays using rabbit derived sera as reference material.
KW  - Monoclonal antibody
KW  - Detection
KW  - Autoimmune diagnostics
KW  - Cross reactivity
KW  - Assay calibration
Y1  - 2019
U6  - https://doi.org/10.1016/j.cca.2019.09.003
SN  - 0009-8981
SN  - 1873-3492
VL  - 499
SP  - 87
EP  - 92
PB  - Elsevier
CY  - Amsterdam
ER  -