TY  - JOUR
A1  - Othman, Abdelmageed M.
A1  - Wollenberger, Ulla
T1  - Amperometric biosensor based on coupling aminated laccase to functionalized carbon nanotubes for phenolics detection
JF  - International journal of biological macromolecules
N2  - A biosensor for phenolic compounds based on a chemically modified laccase from Coriolus hirsula immobilized on functionalized screen-printed carbon electrodes (SPCEs) was achieved. Different enzyme modifications and immobilization strategies were analyzed. The electrochemical response of the immobilized laccase on SPCEs modified with carboxyl functionalized multi-walled carbon nanotubes (COOH-MWCNT) was the highest when laccase was aminated prior to the adsorption onto the working electrode. The developed lactase biosensor sensitivity toward different phenolic compounds was assessed to determine the biosensor response with several phenolic compounds. The highest response was obtained for ABTS with a saturation value of I-max = 27.94 mu A. The electrocatalytic efficiency (I-max/K-m(app)) was the highest for ABTS (5588 mu A mu M-1) followed by syringaldazine (3014 mu A.mu M-1). The sensors were considerably stable, whereby 99.5, 82 and 77% of the catalytic response using catechol as substrate was retained after 4, 8 and 10 successive cycles of reuse respectively, with response time average of 5 s for 12 cycles. No loss of activity was observed after 20 days of storage.
KW  - amperometry
KW  - laccase
KW  - amination
KW  - biosensor
KW  - carbon nanotubes
KW  - phenols
Y1  - 2020
U6  - https://doi.org/10.1016/j.ijbiomac.2020.03.049
SN  - 0141-8130
SN  - 1879-0003
VL  - 153
SP  - 855
EP  - 864
PB  - Elsevier
CY  - New York, NY [u.a.]
ER  -