TY - JOUR A1 - Weingart, C. A1 - Raila, Jens A1 - Lübke-Becker, A. A1 - Kershaw, O. A1 - Brunnberg, M. A1 - Kohn, B. T1 - Calcitriol induced hypercalcemia in a hunting dog with a disseminated Paecilomyces variotii infection T1 - Calcitriol-bedingte Hyperkalzämie bei einem Jagdhund mit disseminierter Paecilomyces variotii-Infektion JF - Schweizer Archiv für Tierheilkunde N2 - A 5-year old hunting dog was presented with reduced appetite, weight loss and polyuria/polydipsia. Hematology and clinical chemistry revealed anemia, leukocytosis, increased liver enzymes, hypoalbuminemia and hypercalcemia. The cytological, pathohistological and microbiological examination identified a disseminated infection with the saprophytic mould fungus Paecilomyces variotii in the biopsies of the spleen and a lymph node. Determination of vitamin D metabolites confirmed a calcitriol induced hypercalcemia. N2 - Ein 5-jähriger Jagdhund wurde wegen verminderter Futteraufnahme, Gewichtsverlust und Polyurie/Polydipsie vorgestellt. In der hämatologischen und klinisch-chemischen Blutuntersuchung wurde neben einer Anämie und Leukozytose eine Erhöhung der Leberenzyme, Hypoalbuminämie und Hyperkalzämie festgestellt. Durch zytologische, pathohistologische und mikrobiologische Untersuchungen von Biopsien aus Milz und Lymphknoten konnte eine systemische Schimmelpilzinfektion mit Paecilomyces variotii nachgewiesen werden. Die Bestimmung der Vitamin-D-Metabolite bestätigte das Vorliegen einer Hyperkalzämie infolge einer Erhöhung der Calcitriolkonzentration. KW - mould fungus KW - calcium KW - polyuria/polydipsia KW - dog KW - Schimmelpilzinfektion KW - Kalzium KW - Polyurie/ Polydipsie KW - Hund Y1 - 2018 U6 - https://doi.org/10.17236/sat00161 SN - 0036-7281 SN - 1664-2848 IS - 5 SP - 313 EP - 319 PB - Gesellschaft Schweizer Tierärztinnen und Tierärzte CY - Bern ET - 160 ER - TY - JOUR A1 - Kobel-Höller, Konstanze A1 - Gley, Kevin A1 - Jochinke, Janina A1 - Heider, Kristina A1 - Fritsch, Verena Nadin A1 - Ha Viet Duc Nguyen, A1 - Lischke, Timo A1 - Radek, Renate A1 - Baumgrass, Ria A1 - Mutzel, Rupert A1 - Thewes, Sascha T1 - Calcineurin Silencing in Dictyostelium discoideum Leads to Cellular Alterations Affecting Mitochondria, Gene Expression, and Oxidative Stress Response JF - Protist N2 - Calcineurin is involved in development and cell differentiation of the social amoeba Dictyostelium discoideum. However, since knockouts of the calcineurin-encoding genes are not possible in D. discoideum it is assumed that the phosphatase also plays a crucial role during vegetative growth of the amoebae. Therefore, we investigated the role of calcineurin during vegetative growth in D. discoideum. RNAi-silenced calcineurin mutants showed cellular alterations with an abnormal morphology of mitochondria and had increased content of mitochondrial DNA (mtDNA). In contrast, mitochondria showed no substantial functional impairment. Calcineurin-silencing led to altered expression of calcium-regulated genes as well as mitochondrially-encoded genes. Furthermore, genes related to oxidative stress were higher expressed in the mutants, which correlated to an increased resistance towards reactive oxygen species (ROS). Most of the changes observed during vegetative growth were not seen after starvation of the calcineurin mutants. We show that impairment of calcineurin led to many subtle, but in the sum crucial cellular alterations in vegetative D. discoideum cells. As these alterations were not observed after starvation we propose a dual role for calcineurin during growth and development. Our results imply that calcineurin is one player in the mutual interplay between mitochondria and ROS during vegetative growth. KW - mtDNA KW - mitochondrial remodelling KW - calcium KW - oxidative stress KW - phototaxis Y1 - 2018 U6 - https://doi.org/10.1016/j.protis.2018.04.004 SN - 1434-4610 VL - 169 IS - 4 SP - 584 EP - 602 PB - Elsevier GMBH CY - München ER - TY - JOUR A1 - Glosse, Philipp A1 - Feger, Martina A1 - Mutig, Kerim A1 - Chen, Hong A1 - Hirche, Frank A1 - Hasan, Ahmed Abdallah Abdalrahman Mohamed A1 - Gaballa, Mohamed Mahmoud Salem Ahmed A1 - Hocher, Berthold A1 - Lang, Florian A1 - Föller, Michael T1 - AMP-activated kinase is a regulator of fibroblast growth factor 23 production JF - Kidney international : official journal of the International Society of Nephrology N2 - Fibroblast growth factor 23 (FGF23) is a proteohormone regulating renal phosphate transport and vitamin D metabolism as well as inducing left heart hypertrophy. FGF23-deficient mice suffer from severe tissue calcification, accelerated aging and a myriad of aging-associated diseases. Bone cells produce FGF23 upon store-operated calcium ion entry (SOCE) through the calcium selective ion channel Orai1. AMP-activated kinase (AMPK) is a powerful energy sensor helping cells survive states of energy deficiency, and AMPK down-regulates Orai1. Here we investigated the role of AMPK in FGF23 production. Fgf23 gene transcription was analyzed by qRT-PCR and SOCE by fluorescence optics in UMR106 osteoblast-like cells while the serum FGF23 concentration and phosphate metabolism were assessed in AMPKa1-knockout and wild-type mice. The AMPK activator, 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) down-regulated, whereas the AMPK inhibitor, dorsomorphin dihydrochloride (compound C) and AMPK gene silencing induced Fgf23 transcription. AICAR decreased membrane abundance of Orai1 and SOCE. SOCE inhibitors lowered Fgf23 gene expression induced by AMPK inhibition. AMPKa1-knockout mice had a higher serum FGF23 concentration compared to wild-type mice. Thus, AMPK participates in the regulation of FGF23 production in vitro and in vivo. The inhibitory effect of AMPK on FGF23 production is at least in part mediated by Orai1-involving SOCE. KW - calcium KW - FGF23 KW - Klotho KW - Orai1 KW - phosphate KW - parathyroid hormone Y1 - 2018 U6 - https://doi.org/10.1016/j.kint.2018.03.006 SN - 0085-2538 SN - 1523-1755 VL - 94 IS - 3 SP - 491 EP - 501 PB - Elsevier CY - New York ER -