TY - JOUR A1 - Neugart, Susanne A1 - Wiesner-Reinhold, Melanie A1 - Frede, Katja A1 - Jander, Elisabeth A1 - Homann, Thomas A1 - Rawel, Harshadrai Manilal A1 - Schreiner, Monika A1 - Baldermann, Susanne T1 - Effect of Solid Biological Waste Compost on the Metabolite Profile of Brassica rapa ssp chinensis JF - Frontiers in plant science : FPLS N2 - Large quantities of biological waste are generated at various steps within the food production chain and a great utilization potential for this solid biological waste exists apart from the current main usage for the feedstuff sector. It remains unclear how the usage of biological waste as compost modulates plant metabolites. We investigated the effect of biological waste of the processing of coffee, aronia, and hop added to soil on the plant metabolite profile by means of liquid chromatography in pak choi sprouts. Here we demonstrate that the solid biological waste composts induced specific changes in the metabolite profiles and the changes are depending on the type of the organic residues and its concentration in soil. The targeted analysis of selected plant metabolites, associated with health beneficial properties of the Brassicaceae family, revealed increased concentrations of carotenoids (up to 3.2-fold) and decreased amounts of glucosinolates (up to 4.7-fold) as well as phenolic compounds (up to 1.5-fold). KW - metabolite profiling KW - LC/MS KW - pak choi KW - carotenoids KW - phenolic compounds KW - glucosinolates Y1 - 2018 U6 - https://doi.org/10.3389/fpls.2018.00305 SN - 1664-462X VL - 9 PB - Frontiers Media CY - Lausanne ER - TY - JOUR A1 - Frede, Katja A1 - Schreiner, Monika A1 - Zrenner, R. A1 - Graefe, Jan A1 - Baldermann, Susanne T1 - Carotenoid biosynthesis of pak choi (Brassica rapa ssp chinensis) sprouts grown under different light-emitting diodes during the diurnal course JF - Photochemical & photobiological sciences N2 - Light-emitting diodes (LEDs) are considered the future of greenhouse lighting. This study investigates the carotenoid concentrations of pak choi sprouts after growth under blue, red and white LEDs at six different time points. Furthermore, the diurnal changes of RNA transcripts of key genes of the carotenoid biosynthesis pathway as well as of the carotenoid cleavage dioxygenase 4 (CCD4) gene and of the transcription factor genes elongated hypocotyl 5 (HY5) and circadian clock associated 1 (CCA1) were investigated. The carotenoid concentrations were steady throughout the day, but showed a small maximum in the afternoon. An average total carotenoid concentration of 536 +/- 29 ng mg(-1) DM produced under white LEDs was measured, which is comparable to previously described field-grown levels. The carotenoid concentrations were slightly lower under blue or red LEDs. Moreover, the diurnal RNA transcript rhythms of most of the carotenoid biosynthesis genes showed an increase during the light period, which can be correlated to the carotenoid maxima in the afternoon. Blue LEDs caused the highest transcriptional induction of biosynthetic genes as well as of CCD4, thereby indicating an increased flux through the pathway. In addition, the highest levels of HY5 transcripts and CCA1 transcripts were determined under blue LEDs. Y1 - 2018 U6 - https://doi.org/10.1039/c8pp00136g SN - 1474-905X SN - 1474-9092 VL - 17 IS - 10 SP - 1289 EP - 1300 PB - Royal Society of Chemistry CY - Cambridge ER -