TY  - JOUR
A1  - Durgud, Meriem
A1  - Gupta, Saurabh
A1  - Ivanov, Ivan
A1  - Omidbakhshfard, Mohammad Amin
A1  - Benina, Maria
A1  - Alseekh, Saleh
A1  - Staykov, Nikola
A1  - Hauenstein, Mareike
A1  - Dijkwel, Paul P.
A1  - Hortensteiner, Stefan
A1  - Toneva, Valentina
A1  - Brotman, Yariv
A1  - Fernie, Alisdair R.
A1  - Müller-Röber, Bernd
A1  - Gechev, Tsanko S.
T1  - Molecular Mechanisms Preventing Senescence in Response to Prolonged Darkness in a Desiccation-Tolerant Plant
JF  - Plant physiology : an international journal devoted to physiology, biochemistry, cellular and molecular biology, biophysics and environmental biology of plants
N2  - The desiccation-tolerant plant Haberlea rhodopensis can withstand months of darkness without any visible senescence. Here, we investigated the molecular mechanisms of this adaptation to prolonged (30 d) darkness and subsequent return to light. H. rhodopensis plants remained green and viable throughout the dark treatment. Transcriptomic analysis revealed that darkness regulated several transcription factor (TF) genes. Stress-and autophagy-related TFs such as ERF8, HSFA2b, RD26, TGA1, and WRKY33 were up-regulated, while chloroplast-and flowering-related TFs such as ATH1, COL2, COL4, RL1, and PTAC7 were repressed. PHYTOCHROME INTERACTING FACTOR4, a negative regulator of photomorphogenesis and promoter of senescence, also was down-regulated. In response to darkness, most of the photosynthesis-and photorespiratory-related genes were strongly down-regulated, while genes related to autophagy were up-regulated. This occurred concomitant with the induction of SUCROSE NON-FERMENTING1-RELATED PROTEIN KINASES (SnRK1) signaling pathway genes, which regulate responses to stress-induced starvation and autophagy. Most of the genes associated with chlorophyll catabolism, which are induced by darkness in dark-senescing species, were either unregulated (PHEOPHORBIDE A OXYGENASE, PAO; RED CHLOROPHYLL CATABOLITE REDUCTASE, RCCR) or repressed (STAY GREEN-LIKE, PHEOPHYTINASE, and NON-YELLOW COLORING1). Metabolite profiling revealed increases in the levels of many amino acids in darkness, suggesting increased protein degradation. In darkness, levels of the chloroplastic lipids digalactosyldiacylglycerol, monogalactosyldiacylglycerol, phosphatidylglycerol, and sulfoquinovosyldiacylglycerol decreased, while those of storage triacylglycerols increased, suggesting degradation of chloroplast membrane lipids and their conversion to triacylglycerols for use as energy and carbon sources. Collectively, these data show a coordinated response to darkness, including repression of photosynthetic, photorespiratory, flowering, and chlorophyll catabolic genes, induction of autophagy and SnRK1 pathways, and metabolic reconfigurations that enable survival under prolonged darkness.
Y1  - 2018
U6  - https://doi.org/10.1104/pp.18.00055
SN  - 0032-0889
SN  - 1532-2548
VL  - 177
IS  - 3
SP  - 1319
EP  - 1338
PB  - American Society of Plant Physiologists
CY  - Rockville
ER  - 
TY  - JOUR
A1  - Rodriguez Cubillos, Andres Eduardo
A1  - Tong, Hao
A1  - Alseekh, Saleh
A1  - de Abreu e Lima, Francisco Anastacio
A1  - Yu, Jing
A1  - Fernie, Alisdair R.
A1  - Nikoloski, Zoran
A1  - Laitinen, Roosa A. E.
T1  - Inheritance patterns in metabolism and growth in diallel crosses of Arabidopsis thaliana from a single growth habitat
JF  - Heredity
N2  - Metabolism is a key determinant of plant growth and modulates plant adaptive responses. Increased metabolic variation due to heterozygosity may be beneficial for highly homozygous plants if their progeny is to respond to sudden changes in the habitat. Here, we investigate the extent to which heterozygosity contributes to the variation in metabolism and size of hybrids of Arabidopsis thaliana whose parents are from a single growth habitat. We created full diallel crosses among seven parents, originating from Southern Germany, and analysed the inheritance patterns in primary and secondary metabolism as well as in rosette size in situ. In comparison to primary metabolites, compounds from secondary metabolism were more variable and showed more pronounced non-additive inheritance patterns which could be attributed to epistasis. In addition, we showed that glucosinolates, among other secondary metabolites, were positively correlated with a proxy for plant size. Therefore, our study demonstrates that heterozygosity in local A. thaliana population generates metabolic variation and may impact several tasks directly linked to metabolism.
Y1  - 2018
U6  - https://doi.org/10.1038/s41437-017-0030-5
SN  - 0018-067X
SN  - 1365-2540
VL  - 120
IS  - 5
SP  - 463
EP  - 473
PB  - Nature Publ. Group
CY  - London
ER  -