TY  - JOUR
A1  - Hartmann, Stefanie
A1  - Hasenkamp, Natascha
A1  - Mayer, Jens
A1  - Michaux, Johan
A1  - Morand, Serge
A1  - Mazzoni, Camila J.
A1  - Roca, Alfred L.
A1  - Greenwood, Alex D.
T1  - Endogenous murine leukemia retroviral variation across wild European and inbred strains of house mouse
JF  - BMC genomics
N2  - Background: Endogenous murine leukemia retroviruses (MLVs) are high copy number proviral elements difficult to comprehensively characterize using standard low throughput sequencing approaches. However, high throughput approaches generate data that is challenging to process, interpret and present.
 Results: Next generation sequencing (NGS) data was generated for MLVs from two wild caught Mus musculus domesticus (from mainland France and Corsica) and for inbred laboratory mouse strains C3H, LP/J and SJL. Sequence reads were grouped using a novel sequence clustering approach as applied to retroviral sequences. A Markov cluster algorithm was employed, and the sequence reads were queried for matches to specific xenotropic (Xmv), polytropic (Pmv) and modified polytropic (Mpmv) viral reference sequences.
 Conclusions: Various MLV subtypes were more widespread than expected among the mice, which may be due to the higher coverage of NGS, or to the presence of similar sequence across many different proviral loci. The results did not correlate with variation in the major MLV receptor Xpr1, which can restrict exogenous MLVs, suggesting that endogenous MLV distribution may reflect gene flow more than past resistance to infection.
KW  - Murine leukemia virus
KW  - Endogenous retrovirus
KW  - Xpr1
KW  - XMRV
KW  - Genomic evolution
KW  - Markov cluster algorithm
Y1  - 2015
U6  - https://doi.org/10.1186/s12864-015-1766-z
SN  - 1471-2164
VL  - 16
PB  - BioMed Central
CY  - London
ER  -