TY  - JOUR
A1  - Berry, Scott
A1  - Rosa, Stefanie
A1  - Howard, Martin
A1  - Buhler, Marc
A1  - Dean, Caroline
T1  - Disruption of an RNA-binding hinge region abolishes LHP1-mediated epigenetic repression
JF  - Genes & Development
N2  - Epigenetic maintenance of gene repression is essential for development. Polycomb complexes are central to this memory, but many aspects of the underlying mechanism remain unclear. LIKE HETEROCHROMATIN PROTEIN 1 (LHP1) binds Polycomb-deposited H3K27me3 and is required for repression of many Polycomb target genes in Arabidopsis. Here we show that LHP1 binds RNA in vitro through the intrinsically disordered hinge region. By independently perturbing the RNA-binding hinge region and H3K27me3 (trimethylation of histone H3 at Lys27) recognition, we found that both facilitate LHP1 localization and H3K27me3 maintenance. Disruption of the RNAbinding hinge region also prevented formation of subnuclear foci, structures potentially important for epigenetic repression.
KW  - chromatin
KW  - epigenetics
KW  - plant biology
KW  - Polycomb
KW  - RNA
Y1  - 2017
U6  - https://doi.org/10.1101/gad.305227.117
SN  - 0890-9369
SN  - 1549-5477
VL  - 31
SP  - 2115
EP  - 2120
PB  - Cold Spring Harbor Laboratory Press
CY  - Cold Spring Harbor, NY
ER  - 
TY  - GEN
A1  - Rajasundaram, Dhivyaa
A1  - Selbig, Joachim
T1  - More effort — more results
BT  - recent advances in integrative ‘omics’ data analysis
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - The development of 'omics' technologies has progressed to address complex biological questions that underlie various plant functions thereby producing copious amounts of data. The need to assimilate large amounts of data into biologically meaningful interpretations has necessitated the development of statistical methods to integrate multidimensional information. Throughout this review, we provide examples of recent outcomes of 'omics' data integration together with an overview of available statistical methods and tools.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 923 
KW  - principal component
KW  - plant biology
KW  - package
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-442639
SN  - 1866-8372
IS  - 923
SP  - 57
EP  - 61
ER  -