TY  - GEN
A1  - Thirumalaikumar, Venkatesh P.
A1  - Devkar, Vikas
A1  - Mehterov, Nikolay
A1  - Ali, Shawkat
A1  - Ozgur, Rengin
A1  - Turkan, Ismail
A1  - Müller-Röber, Bernd
A1  - Balazadeh, Salma
T1  - NAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomato
T2  - Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe
N2  - Water deficit (drought stress) massively restricts plant growth and the yield of crops; reducing the deleterious effects of drought is therefore of high agricultural relevance. Drought triggers diverse cellular processes including the inhibition of photosynthesis, the accumulation of cell‐damaging reactive oxygen species and gene expression reprogramming, besides others. Transcription factors (TF) are central regulators of transcriptional reprogramming and expression of many TF genes is affected by drought, including members of the NAC family. Here, we identify the NAC factor JUNGBRUNNEN1 (JUB1) as a regulator of drought tolerance in tomato (Solanum lycopersicum). Expression of tomato JUB1 (SlJUB1) is enhanced by various abiotic stresses, including drought. Inhibiting SlJUB1 by virus‐induced gene silencing drastically lowers drought tolerance concomitant with an increase in ion leakage, an elevation of hydrogen peroxide (H2O2) levels and a decrease in the expression of various drought‐responsive genes. In contrast, overexpression of AtJUB1 from Arabidopsis thaliana increases drought tolerance in tomato, alongside with a higher relative leaf water content during drought and reduced H2O2 levels. AtJUB1 was previously shown to stimulate expression of DREB2A, a TF involved in drought responses, and of the DELLA genes GAI and RGL1. We show here that SlJUB1 similarly controls the expression of the tomato orthologs SlDREB1, SlDREB2 and SlDELLA. Furthermore, AtJUB1 directly binds to the promoters of SlDREB1, SlDREB2 and SlDELLA in tomato. Our study highlights JUB1 as a transcriptional regulator of drought tolerance and suggests considerable conservation of the abiotic stress‐related gene regulatory networks controlled by this NAC factor between Arabidopsis and tomato.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 568 
KW  - Arabidopsis
KW  - tomato
KW  - transcription factor
KW  - drought
KW  - reactive oxygen species
KW  - DELLA
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-423908
SN  - 1866-8372
IS  - 568
ER  - 
TY  - THES
A1  - Bäßler, Olivia
T1  - Identifizierung und Charakterisierung IgE- reaktiver Proteine in der Tomate (Lycopersicon esculentum)
T1  - Novel tomato allergens : IgE-reactive legumin and vicilin proteins identified by multidimensional protein fractionation ; mass spectrometry and in silico epitope modelling
N2  - Zur Detektion neuer IgE- reaktiver Proteine wurde in dieser Arbeit ein zweidimensionales Proteintrennverfahren verwendet. Resultierende Proteinfraktionen wurden mithilfe von 18 tomatensensibiliesierten Patientenseren im Immunoblot getestet. Detektierte Proteine in der SDS-PAGE wurden mittels LC-MS/MS identifiziert. Dadurch konnten 2 Tomatensamenproteine, die im Immunoblot ein IgE- reaktives Signal zeigten eindeutig mittels Massenspektrometrie identifiziert werden. Diese Proteine sind Legumin und Vicilin. Durch Sequenzabgleich und Proteinstrukturmodellierung im Vergleich zu bereits bekannten Allergenen (Erdnuss und Cashewnuss), konnte eine hohe Homologie gezeigt werden.
N2  - For the detection of tomato allergens a multidimensional protein fractionation strategy and LC-MS/MS was used. Putative allergens were detected by IgE immunoblotting using sera from 18 adult tomato sensitised patients selected based on a positive history skin prick test and specific Immunglobulin (Ig) E levels. Two legumin- and vicilin- proteins were purified and showed strong IgE-reactivity in immunoblots. Individual patient sera exhibited varying IgE-sensitivity against the purified proteins. In silico structural modelling indicates high homology between epitopes of known peanut and cashewnut allergens and the detected IgE-crossreactive tomato proteins.
KW  - Massenspektrometrie
KW  - Tomate
KW  - Nahrungsmittelallergie
KW  - Speicherproteine
KW  - mass spectrometry
KW  - tomato
KW  - food allergy
KW  - storage proteins
Y1  - 2008
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-26953
ER  -