TY  - GEN
A1  - Hofreiter, Michael
A1  - Paijmans, Johanna L. A.
A1  - Goodchild, Helen
A1  - Speller, Camilla F.
A1  - Barlow, Axel
A1  - Gonzalez-Fortes, Gloria M.
A1  - Thomas, Jessica A.
A1  - Ludwig, Arne
A1  - Collins, Matthew J.
T1  - The future of ancient DNA
BT  - technical advances and conceptual shifts
T2  - Postprints der Universität Potsdam : Mathematisch Naturwissenschaftliche Reihe
N2  - Technological innovations such as next generation sequencing and DNA hybridisation enrichment have resulted in multi-fold increases in both the quantity of ancient DNA sequence data and the time depth for DNA retrieval. To date, over 30 ancient genomes have been sequenced, moving from 0.7x coverage (mammoth) in 2008 to more than 50x coverage (Neanderthal) in 2014. Studies of rapid evolutionary changes, such as the evolution and spread of pathogens and the genetic responses of hosts, or the genetics of domestication and climatic adaptation, are developing swiftly and the importance of palaeogenomics for investigating evolutionary processes during the last million years is likely to increase considerably. However, these new datasets require new methods of data processing and analysis, as well as conceptual changes in interpreting the results. In this review we highlight important areas of future technical and conceptual progress and discuss research topics in the rapidly growing field of palaeogenomics.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 908 
KW  - ancient DNA
KW  - hybridisation capture
KW  - multi-locus data
KW  - next generation sequencing (NGS)
KW  - palaeogenomics
KW  - population genomics
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-438816
SN  - 1866-8372
IS  - 908
SP  - 284
EP  - 295
ER  - 
TY  - GEN
A1  - Westbury, Michael V.
A1  - Hartmann, Stefanie
A1  - Barlow, Axel
A1  - Wiesel, Ingrid
A1  - Leo, Viyanna
A1  - Welch, Rebecca
A1  - Parker, Daniel M.
A1  - Sicks, Florian
A1  - Ludwig, Arne
A1  - Dalen, Love
A1  - Hofreiter, Michael
T1  - Extended and continuous decline in effective population size results in low genomic diversity in the world's rarest hyena species, the brown hyena
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Hyenas (family Hyaenidae), as the sister group to cats (family Felidae), represent a deeply diverging branch within the cat-like carnivores (Feliformia). With an estimated population size of <10,000 individuals worldwide, the brown hyena (Parahyaena brunnea) represents the rarest of the four extant hyena species and has been listed as Near Threatened by the IUCN. Here, we report a high-coverage genome from a captive bred brown hyena and both mitochondrial and low-coverage nuclear genomes of 14 wild-caught brown hyena individuals from across southern Africa. We find that brown hyena harbor extremely low genetic diversity on both the mitochondrial and nuclear level, most likely resulting from a continuous and ongoing decline in effective population size that started similar to 1 Ma and dramatically accelerated towards the end of the Pleistocene. Despite the strikingly low genetic diversity, we find no evidence of inbreeding within the captive bred individual and reveal phylogeographic structure, suggesting the existence of several potential subpopulations within the species.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 589 
KW  - evolution
KW  - hyena
KW  - genomics
KW  - population genomics
KW  - diversity
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-414132
SN  - 1866-8372
IS  - 589
ER  -