TY  - JOUR
A1  - Eisold, Ursula
A1  - Kupstat, Annette
A1  - Klier, Dennis Tobias
A1  - Primus, Philipp-A.
A1  - Pschenitza, Michael
A1  - Niessner, Reinhard
A1  - Knopp, Dietmar
A1  - Kumke, Michael Uwe
T1  - Probing the physicochemical interactions of 3-hydroxy-benzo[a]pyrene with different monoclonal and recombinant antibodies by use of fluorescence line-narrowing spectroscopy
JF  - Analytical & bioanalytical chemistry
N2  - Characterization of interactions between antigens and antibodies is of utmost importance both for fundamental understanding of the binding and for development of advanced clinical diagnostics. Here, fluorescence line-narrowing (FLN) spectroscopy was used to study physicochemical interactions between 3-hydroxybenzo[a]pyrene (3OH-BaP, as antigen) and a variety of solvent matrices (as model systems) or anti-polycyclic aromatic hydrocarbon antibodies (anti-PAH). We focused the studies on the specific physicochemical interactions between 3OH-BaP and different, previously obtained, monoclonal and recombinant anti-PAH antibodies. Control experiments performed with non-binding monoclonal antibodies and bovine serum albumin (BSA) indicated that nonspecific interactions did not affect the FLN spectrum of 3OH-BaP. The spectral positions and relative intensities of the bands in the FLN spectra are highly dependent on the molecular environment of the 3OH-BaP. The FLN bands correlate with different vibrational modes of 3OH-BaP which are affected by interactions with the molecular environment (pi-pi interactions, H-bonding, or van-der-Waals forces). Although the analyte (3OH-BaP) was the same for all the antibodies investigated, different binding interactions could be identified from the FLN spectra on the basis of structural flexibility and conformational multiplicity of the antibodies' paratopes.
KW  - FLNS
KW  - Antibody
KW  - Paratope
KW  - Hapten
KW  - Polycyclic aromatic hydrocarbons
Y1  - 2014
U6  - https://doi.org/10.1007/s00216-013-7584-8
SN  - 1618-2642
SN  - 1618-2650
VL  - 406
IS  - 14
SP  - 3387
EP  - 3394
PB  - Springer
CY  - Heidelberg
ER  -