TY  - JOUR
A1  - Fernando, Raquel
A1  - Drescher, Cathleen
A1  - Deubel, Stefanie
A1  - Jung, Tobias
A1  - Ost, Mario
A1  - Klaus, Susanne
A1  - Grune, Tilman
A1  - Castro, Jose Pedro
T1  - Low proteasomal activity in fast skeletal muscle fibers is not associated with increased age-related oxidative damage
JF  - Experimental gerontology
N2  - The skeletal muscle is a crucial tissue for maintaining whole body homeostasis. Aging seems to have a disruptive effect on skeletal muscle homeostasis including proteostasis. However, how aging specifically impacts slow and fast twitch fiber types remains elusive. Muscle proteostasis is largely maintained by the proteasomal system. Here we characterized the proteasomal system in two different fiber types, using a non-sarcopenic aging model. By analyzing the proteasomal activity and amount, as well as the polyubiquitinated proteins and the level of protein oxidation in Musculus soleus (Sol) and Musculus extensor digitorum longus (EDL), we found that the slow twitch Sol muscle shows an overall higher respiratory and proteasomal activity in young and old animals. However, especially during aging the fast twitch EDL muscle reduces protein oxidation by an increase of antioxidant capacity. Thus, under adaptive non-sarcopenic conditions, the two fibers types seem to have different strategies to avoid age-related changes.
KW  - Proteasomal system
KW  - Skeletal muscle
KW  - Fast and slow fibers
KW  - Polyubiquitination
KW  - Oxidized proteins
KW  - Antioxidants
KW  - Aging
KW  - Mitochondrial respiration
Y1  - 2018
U6  - https://doi.org/10.1016/j.exger.2018.10.018
SN  - 0531-5565
SN  - 1873-6815
VL  - 117
SP  - 45
EP  - 52
PB  - Elsevier
CY  - Oxford
ER  -