TY  - JOUR
A1  - Schulz, Eike C.
A1  - Mehrabi, Pedram
A1  - Müller-Werkmeister, Henrike
A1  - Tellkamp, Friedjof
A1  - Jha, Ajay
A1  - Stuart, William
A1  - Persch, Elke
A1  - De Gasparo, Raoul
A1  - Diederich, François
A1  - Pai, Emil F.
A1  - Miller, R. J. Dwayne
T1  - The hit-and-return system enables efficient time-resolved serial synchrotron crystallography
JF  - Nature methods : techniques for life scientists and chemists
N2  - We present a ‘hit-and-return’ (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.
KW  - Biophysical chemistry
KW  - Enzymes
KW  - Molecular biophysics
KW  - X-ray crystallography
Y1  - 2018
U6  - https://doi.org/10.1038/s41592-018-0180-2
SN  - 1548-7091
SN  - 1548-7105
VL  - 15
IS  - 11
SP  - 901
EP  - 904
PB  - Nature Publishing Group (London)
CY  - London
ER  -