TY  - JOUR
A1  - Wüstenhagen, Doreen Anja
A1  - Lukas, Phil
A1  - Müller, Christian
A1  - Aubele, Simone A.
A1  - Hildebrandt, Jan-Peter
A1  - Kubick, Stefan
T1  - Cell-free synthesis of the hirudin variant 1 of the blood-sucking leech Hirudo medicinalis
JF  - Scientific reports
N2  - Synthesis and purification of peptide drugs for medical applications is a challenging task. The leech-derived factor hirudin is in clinical use as an alternative to heparin in anticoagulatory therapies. So far, recombinant hirudin is mainly produced in bacterial or yeast expression systems. We describe the successful development and application of an alternative protocol for the synthesis of active hirudin based on a cell-free protein synthesis approach. Three different cell lysates were compared, and the effects of two different signal peptide sequences on the synthesis of mature hirudin were determined. The combination of K562 cell lysates and the endogenous wild-type signal peptide sequence was most effective. Cell-free synthesized hirudin showed a considerably higher anti-thrombin activity compared to recombinant hirudin produced in bacterial cells.
Y1  - 2020
U6  - https://doi.org/10.1038/s41598-020-76715-w
SN  - 2045-2322
VL  - 10
IS  - 1
PB  - Macmillan Publishers Limited, part of Springer Nature
CY  - London
ER  -