39570
2017
2017
eng
14
article
BioMed Central
London
1
--
2017-04-20
--
Morphogenesis of honeybee hypopharyngeal gland during pupal development
Background
The hypopharyngeal gland of worker bees contributes to the production of the royal jelly fed to queens and larvae. The gland consists of thousands of two-cell units that are composed of a secretory cell and a duct cell and that are arranged in sets of about 12 around a long collecting duct.
Results
By fluorescent staining, we have examined the morphogenesis of the hypopharyngeal gland during pupal life, from a saccule lined by a pseudostratified epithelium to the elaborate organ of adult worker bees. The hypopharyngeal gland develops as follows. (1) Cell proliferation occurs during the first day of pupal life in the hypopharyngeal gland primordium. (2) Subsequently, the epithelium becomes organized into rosette-like units of three cells. Two of these will become the secretory cell and the duct cell of the adult secretory units; the third cell contributes only temporarily to the development of the secretory units and is eliminated by apoptosis in the second half of pupal life. (3) The three-cell units of flask-shaped cells undergo complex changes in cell morphology. Thus, by mid-pupal stage, the gland is structurally similar to the adult hypopharyngeal gland. (4) Concomitantly, the prospective secretory cell attains its characteristic subcellular organization by the invagination of a small patch of apical membrane domain, its extension to a tube of about 100 μm in length (termed a canaliculus), and the expansion of the tube to a diameter of about 3 μm. (6) Finally, the canaliculus-associated F-actin system becomes reorganized into rings of bundled actin filaments that are positioned at regular distances along the membrane tube.
Conclusions
The morphogenesis of the secretory units in the hypopharyngeal gland of the worker bee seems to be based on a developmental program that is conserved, with slight modification, among insects for the production of dermal glands. Elaboration of the secretory cell as a unicellular seamless epithelial tube occurs by invagination of the apical membrane, its extension likely by targeted exocytosis and its expansion, and finally the reorganisation of the membrane-associated F-actin system. Our work is fundamental for future studies of environmental effects on hypopharyngeal gland morphology and development.
Frontiers in zoology
10.1186/s12983-017-0207-z
1742-9994
Universität Potsdam, Publikationsfonds
PA 2017_17
1832.60
online registration
22
<a href="http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-395712">Zweitveröffentlichung in der Schriftenreihe Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe ; 337</a>
CC-BY - Namensnennung 4.0 International
Sascha Peter Klose
Daniel Rolke
Otto Baumann
eng
uncontrolled
Exocrine gland
eng
uncontrolled
Insect
eng
uncontrolled
Epithelial tube
eng
uncontrolled
Organogenesis
eng
uncontrolled
Cell polarity
eng
uncontrolled
Actin cytoskeleton
eng
uncontrolled
Apoptosis
eng
uncontrolled
Invagination
Biowissenschaften; Biologie
Tiere (Zoologie)
Institut für Biochemie und Biologie
Referiert
Publikationsfonds der Universität Potsdam
Open Access
39571
2017
2017
eng
14
postprint
1
--
2017-04-20
--
Morphogenesis of honeybee hypopharyngeal gland during pupal development
Background
The hypopharyngeal gland of worker bees contributes to the production of the royal jelly fed to queens and larvae. The gland consists of thousands of two-cell units that are composed of a secretory cell and a duct cell and that are arranged in sets of about 12 around a long collecting duct.
Results
By fluorescent staining, we have examined the morphogenesis of the hypopharyngeal gland during pupal life, from a saccule lined by a pseudostratified epithelium to the elaborate organ of adult worker bees. The hypopharyngeal gland develops as follows. (1) Cell proliferation occurs during the first day of pupal life in the hypopharyngeal gland primordium. (2) Subsequently, the epithelium becomes organized into rosette-like units of three cells. Two of these will become the secretory cell and the duct cell of the adult secretory units; the third cell contributes only temporarily to the development of the secretory units and is eliminated by apoptosis in the second half of pupal life. (3) The three-cell units of flask-shaped cells undergo complex changes in cell morphology. Thus, by mid-pupal stage, the gland is structurally similar to the adult hypopharyngeal gland. (4) Concomitantly, the prospective secretory cell attains its characteristic subcellular organization by the invagination of a small patch of apical membrane domain, its extension to a tube of about 100 μm in length (termed a canaliculus), and the expansion of the tube to a diameter of about 3 μm. (6) Finally, the canaliculus-associated F-actin system becomes reorganized into rings of bundled actin filaments that are positioned at regular distances along the membrane tube.
Conclusions
The morphogenesis of the secretory units in the hypopharyngeal gland of the worker bee seems to be based on a developmental program that is conserved, with slight modification, among insects for the production of dermal glands. Elaboration of the secretory cell as a unicellular seamless epithelial tube occurs by invagination of the apical membrane, its extension likely by targeted exocytosis and its expansion, and finally the reorganisation of the membrane-associated F-actin system. Our work is fundamental for future studies of environmental effects on hypopharyngeal gland morphology and development.
urn:nbn:de:kobv:517-opus4-395712
online registration
Frontiers in zoology (2017) NR. 14. - DOI: 10.1186/s12983-017-0207-z
<a href="http://publishup.uni-potsdam.de/opus4-ubp/frontdoor/index/index/docId/39570">Bibliographieeintrag der Originalveröffentlichung/Quelle</a>
CC-BY - Namensnennung 4.0 International
Sascha Peter Klose
Daniel Rolke
Otto Baumann
Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
337
eng
uncontrolled
Exocrine gland
eng
uncontrolled
Insect
eng
uncontrolled
Epithelial tube
eng
uncontrolled
Organogenesis
eng
uncontrolled
Cell polarity
eng
uncontrolled
Actin cytoskeleton
eng
uncontrolled
Apoptosis
eng
uncontrolled
Invagination
Biowissenschaften; Biologie
Tiere (Zoologie)
open_access
Institut für Biochemie und Biologie
Referiert
Open Access
Universität Potsdam
https://publishup.uni-potsdam.de/files/39571/pmnr337_online.pdf