4418
2006
eng
postprint
1
2010-08-27
--
--
Aminergic control and modulation of honeybee behaviour
Biogenic amines are important messenger substances in the central nervous system and in peripheral organs of vertebrates and of invertebrates. The honeybee, Apis mellifera, is excellently suited to uncover the functions of biogenic amines in behaviour, because it has an extensive behavioural repertoire, with a number of biogenic amine receptors characterised in this insect. In the honeybee, the biogenic amines dopamine, octopamine, serotonin and tyramine modulate neuronal functions in various ways. Dopamine and serotonin are present in high concentrations in the bee brain, whereas octopamine and tyramine are less abundant. Octopamine is a key molecule for the control of honeybee behaviour. It generally has an arousing effect and leads to higher sensitivity for sensory inputs, better learning performance and increased foraging behaviour. Tyramine has been suggested to act antagonistically to octopamine, but only few experimental data are available for this amine. Dopamine and serotonin often have antagonistic or inhibitory effects as compared to octopamine. Biogenic amines bind to membrane receptors that primarily belong to the large gene-family of GTP-binding (G) protein coupled receptors. Receptor activation leads to transient changes in concentrations of intracellular second messengers such as cAMP, IP3 and/or Ca2+. Although several biogenic amine receptors from the honeybee have been cloned and characterised more recently, many genes still remain to be identified. The availability of the completely sequenced genome of Apis mellifera will contribute substantially to closing this gap. In this review, we will discuss the present knowledge on how biogenic amines and their receptor-mediated cellular responses modulate different behaviours of honeybees including learning processes and division of labour.
urn:nbn:de:kobv:517-opus-46106
4610
Current neuropharmacology. - 4 (2006), 4, S. 259 - 276, DOI 10.2174/157015906778520791
<hr>The article was originally published by Bentham Science Publishers Ltd.:
<br><a href="http://www.ingentaconnect.com/content/ben/cn">
Current Neuropharmacology</a>. - 4 (2006), 4, S. 259-276<br>
ISSN 1570-159X<br>
DOI <a href="http://dx.doi.org/10.2174/157015906778520791">10.2174/157015906778520791</a>
Keine öffentliche Lizenz: Unter Urheberrechtsschutz
Ricarda Scheiner
Arnd Baumann
Wolfgang Blenau
eng
uncontrolled
Serotonin
eng
uncontrolled
dopamine
eng
uncontrolled
octopamine
eng
uncontrolled
tyramine
eng
uncontrolled
honeybee
Biowissenschaften; Biologie
Institut für Biochemie und Biologie
Universität Potsdam
4421
2008
eng
postprint
1
2010-08-27
--
--
Hormone-induced assembly and activation of V-ATPase in blowfly salivary glands is mediated by protein kinase A
The vacuolar H+-ATPase (V-ATPase) in the apical membrane of blowfly (Calliphora vicina) salivary gland cells energizes the secretion of a KCl-rich saliva in response to the neurohormone serotonin (5-HT). We have shown previously that exposure to 5-HT induces a cAMP-mediated reversible assembly of V-0 and V-1 subcomplexes to V-ATPase holoenzymes and increases V-ATPase-driven proton transport. Here, we analyze whether the effect of cAMP on V-ATPase is mediated by protein kinase A (PKA) or exchange protein directly activated by cAMP (Epac), the cAMP target proteins that are present within the salivary glands. Immunofluorescence microscopy shows that PKA activators, but not Epac activators, induce the translocation of V1 components from the cytoplasm to the apical membrane, indicative of an assembly of V-ATPase holoenzymes. Measurements of transepithelial voltage changes and microfluorometric pH measurements at the luminal surface of cells in isolated glands demonstrate further that PKA-activating cAMP analogs increase cation transport to the gland lumen and induce a V-ATPase-dependent luminal acidification, whereas activators of Epac do not. Inhibitors of PKA block the 5-HT-induced V-1 translocation to the apical membrane and the increase in proton transport. We conclude that cAMP exerts its effects on V-ATPase via PKA.
urn:nbn:de:kobv:517-opus-46126
4612
American journal of physiology : cell physiology. - 294 (2008), 1, S. C56 - C65, DOI: 10.1152/ajpcell.00041.2007
<hr>The article was originally published by the American Physiological Society:
<br><a href="http://ajpcell.physiology.org/contents-by-date.0.shtml">
American Journal of Physiology : Cell Physiology</a>. - 294 (2008), 1, S. C56-C65<br>
ISSN 0363-6143<br>
DOI <a href="http://dx.doi.org/10.1152/ajpcell.00041.2007">10.1152/ajpcell.00041.2007</a>
Keine öffentliche Lizenz: Unter Urheberrechtsschutz
Julia Rein
Martin Voss
Wolfgang Blenau
Bernd Walz
Otto Baumann
eng
uncontrolled
Vacuolar h+-atpase
eng
uncontrolled
camp binding-sites
eng
uncontrolled
cyclic-amp
eng
uncontrolled
plasma-membrane
eng
uncontrolled
drosophila-melanogaster
Biowissenschaften; Biologie
Institut für Biochemie und Biologie
Universität Potsdam
4423
2002
eng
postprint
1
2010-08-27
--
--
Behavioural pharmacology of octopamine, tyramine and dopamine in honey bees
In the honey bee, responsiveness to sucrose correlates with many behavioural parameters such as age of first foraging, foraging role and learning. Sucrose responsiveness can be measured using the proboscis extension response (PER) by applying sucrose solutions of increasing concentrations to the antenna of a bee. We tested whether the biogenic amines octopamine, tyramine and dopamine, and the dopamine receptor agonist 2-amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene (6,7-ADTN) can modulate sucrose responsiveness. The compounds were either injected into the thorax or fed in sucrose solution to compare different methods of application. Injection and feeding of tyramine or octopamine significantly increased sucrose responsiveness. Dopamine decreased sucrose responsiveness when injected into the thorax. Feeding of dopamine had no effect. Injection of 6,7-ADTN into the thorax and feeding of 6,7-ADTN reduced sucrose responsiveness significantly. These data demonstrate that sucrose responsiveness in honey bees can be modulated by biogenic amines, which has far reaching consequences for other types of behaviour in this insect. (C) 2002 Elsevier Science B.V. All rights reserved.
urn:nbn:de:kobv:517-opus-44308
4430
Behavioural brain research 136 (2002), 2, S. 545 - 553, DOI 10.1016/S0166-4328(02)00205-X
<hr>The article was originally published by:
<br><a href="http://www.elsevier.com/wps/find/journaldescription.cws_home/622287/descript">
Elsevier</a><br>
<a href="http://www.sciencedirect.com/science/journal/01664328">Behavioural brain research</a>. - 136 (2002), 2, S. 545-553<br>
ISSN 0166-4328<br>
DOI <a href="http://dx.doi.org/10.1016/S0166-4328(02)00205-X"> 10.1016/S0166-4328(02)00205-X</a>
Keine öffentliche Lizenz: Unter Urheberrechtsschutz
Wolfgang Blenau
Ricarda Scheiner
Stephanie Plückhahn
Bahar Oney
Joachim Erber
eng
uncontrolled
Honey bee
eng
uncontrolled
insect
eng
uncontrolled
proboscis extension response
eng
uncontrolled
sucrose responsiveness
eng
uncontrolled
biogenic amines
Biowissenschaften; Biologie
Institut für Biochemie und Biologie
Universität Potsdam
4424
2003
eng
postprint
1
2010-08-27
--
--
Molecular and functional characterization of an octopamine receptor from honeybee (Apis mellifera) brain
Biogenic amines and their receptors regulate and modulate many physiological and behavioural processes in animals. In vertebrates, octopamine is only found in trace amounts and its function as a true neurotransmitter is unclear. In protostomes, however, octopamine can act as neurotransmitter, neuromodulator and neurohormone. In the honeybee, octopamine acts as a neuromodulator and is involved in learning and memory formation. The identification of potential octopamine receptors is decisive for an understanding of the cellular pathways involved in mediating the effects of octopamine. Here we report the cloning and functional characterization of the first octopamine receptor from the honeybee, Apis mellifera . The gene was isolated from a brain-specific cDNA library. It encodes a protein most closely related to octopamine receptors from Drosophila melanogaster and Lymnea stagnalis . Signalling properties of the cloned receptor were studied in transiently transfected human embryonic kidney (HEK) 293 cells. Nanomolar to micromolar concentrations of octopamine induced oscillatory increases in the intracellular Ca2+ concentration. In contrast to octopamine, tyramine only elicited Ca2+ responses at micromolar concentrations. The gene is abundantly expressed in many somata of the honeybee brain, suggesting that this octopamine receptor is involved in the processing of sensory inputs, antennal motor outputs and higher-order brain functions.
urn:nbn:de:kobv:517-opus-44293
4429
Jornal of neurochemistry 86 (2003), 3, S. 725 - 735, DOI 10.1046/j.1471-4159.2003.01876.x
<hr>The definitive version is available at www3.interscience.wiley.com:
<br><a href="http://www3.interscience.wiley.com/journal/118483663/home">
Journal of Neurochemistry</a>. - 86 (2003), 3, S. 725-735<br>
ISSN 0022-3042, 1474-1644<br>
DOI <a href="http://dx.doi.org/10.1046/j.1471-4159.2003.01876.x"> 10.1046/j.1471-4159.2003.01876.x</a>
Keine öffentliche Lizenz: Unter Urheberrechtsschutz
Wolfgang Blenau
Lore Grohmann
Joachim Erber
Paul R. Ebert
Timo Strünker
Arnd Baumann
eng
uncontrolled
Biogenic amine
eng
uncontrolled
Ca2+
eng
uncontrolled
cyclic AMP
eng
uncontrolled
G protein-coupled receptor
eng
uncontrolled
insect
Biowissenschaften; Biologie
Institut für Biochemie und Biologie
Universität Potsdam
31506
2010
2010
eng
article
1
--
--
--
Characterization of the 5-HT1A receptor of the honeybee (Apis mellifera) and involvement of serotonin in phototactic behavior
Serotonin plays a key role in modulating various physiological and behavioral processes in both protostomes and deuterostomes. The vast majority of serotonin receptors belong to the superfamily of G-protein-coupled receptors. We report the cloning of a cDNA from the honeybee (Am5-ht1A) sharing high similarity with members of the 5-HT1 receptor class. Activation of Am5-HT1A by serotonin inhibited the production of cAMP in a dose-dependent manner (EC50 = 16.9 nM). Am5-HT1A was highly expressed in brain regions known to be involved in visual information processing. Using in vivo pharmacology, we could demonstrate that Am5-HT1A receptor ligands had a strong impact on the phototactic behavior of individual bees. The data presented here mark the first comprehensive study-from gene to behavior-of a 5-HT1A receptor in the honeybee, paving the way for the eventual elucidation of additional roles of this receptor subtype in the physiology and behavior of this social insect.
http://www.springerlink.com/content/101193
10.1007/s00018-010-0350-6
1420-682X
allegro:1991-2014
10107821
Cellular and molecular life sciences. - ISSN 1420-682X. - 67 (2010), 14, S. 2467 - 2479
Markus Thamm
Sabine Balfanz
Richarda Scheiner
Arnd Baumann
Wolfgang Blenau
Institut für Biochemie und Biologie
Referiert
31529
2010
2010
eng
article
1
--
--
--
The role of octopamine in locusts and other arthropods
The biogenic amine octopamine and its biological precursor tyramine are thought to be the invertebrate functional homologues of the vertebrate adrenergic transmitters. Octopamine functions as a neuromodulator, neurotransmitter and neurohormone in insect nervous systems and prompts the whole organism to "dynamic action". A growing number of studies suggest a prominent role for octopamine in modulating multiple physiological and behavioural processes in invertebrates, as for example the phase transition in Schistocerca gregaria. Both octopamine and tyramine exert their effects by binding to specific receptor proteins that belong to the superfamily of G protein-coupled receptors. Since these receptors do not appear to be present in vertebrates, they may present very suitable and specific insecticide and acaricide targets. (C) 2010 Elsevier Ltd. All rights reserved.
http://www.sciencedirect.com/science/journal/00221910
10.1016/j.jinsphys.2010.05.018
0022-1910
allegro:1991-2014
10107849
Journal of insect physiology. - ISSN 0022-1910. - 56 (2010), 8, S. 854 - 867
Heleen Verlinden
Rut Vleugels
Elisabeth Marchal
Liesbeth Badisco
Hans-Joachim Pflüger
Wolfgang Blenau
Jozef Vanden Broeck
Institut für Biochemie und Biologie
Referiert
31530
2010
2010
eng
article
1
--
--
--
The cloning, phylogenetic relationship and distribution pattern of two new putative GPCR-type octopamine receptors in the desert locust (Schistocerca gregaria)
The biogenic amine octopamine functions as a neuromodulator, neurotransmitter and neurohormone in insect nervous systems. It plays a prominent role in modulating multiple physiological and behavioural processes in invertebrates. Octopamine exerts its effects by binding to specific receptor proteins that belong to the superfamily of G protein-coupled receptors. We found two partial sequences of putative octopamine receptors in the desert locust Schistocerca gregaria (SgOct alpha R and SgOct beta R) and investigated their transcript levels in males and females of both phases and during the transition between long-term solitarious and gregarious locusts. The transcript levels of SgOctaR are the highest in the central nervous system, whereas those of SgOct beta R are the highest in the flight muscles, followed by the central nervous system. Both SgOct alpha R and SgOct beta R show higher transcript levels in long-term gregarious locusts as compared to solitarious ones. The rise of SgOct beta R transcript levels already appears during the first 4 h of gregarisation, during which also the behavioural changes take place.
http://www.sciencedirect.com/science/journal/00221910
10.1016/j.jinsphys.2010.03.003
0022-1910
allegro:1991-2014
10107850
Journal of insect physiology. - ISSN 0022-1910. - 56 (2010), 8, S. 868 - 875
Heleen Verlinden
Rut Vleugels
Elisabeth Marchal
Liesbeth Badisco
Julie Tobback
Hans-Joachim Pflüger
Wolfgang Blenau
Jozef Vanden Broeck
Institut für Biochemie und Biologie
Referiert
Open Access
34527
2013
2013
eng
12
12
8
article
PLoS
San Fransisco
1
--
--
--
Function and distribution of 5-HT2 receptors in the honeybee (apis mellifera)
Background: Serotonin plays a pivotal role in regulating and modulating physiological and behavioral processes in both vertebrates and invertebrates. In the honeybee (Apis mellifera), serotonin has been implicated in division of labor, visual processing, and learning processes. Here, we present the cloning, heterologous expression, and detailed functional and pharmacological characterization of two honeybee 5-HT2 receptors.
Methods: Honeybee 5-HT2 receptor cDNAs were amplified from brain cDNA. Recombinant cell lines were established constitutively expressing receptor variants. Pharmacological properties of the receptors were investigated by Ca2+ imaging experiments. Quantitative PCR was applied to explore the expression patterns of receptor mRNAs.
Results: The honeybee 5-HT2 receptor class consists of two subtypes, Am5-HT2 alpha and Am5-HT2 beta. Each receptor gene also gives rise to alternatively spliced mRNAs that possibly code for truncated receptors. Only activation of the full-length receptors with serotonin caused an increase in the intracellular Ca2+ concentration. The effect was mimicked by the agonists 5-methoxytryptamine and 8-OH-DPAT at low micromolar concentrations. Receptor activities were blocked by established 5-HT receptor antagonists such as clozapine, methiothepin, or mianserin. High transcript numbers were detected in exocrine glands suggesting that 5-HT2 receptors participate in secretory processes in the honeybee.
Conclusions: This study marks the first molecular and pharmacological characterization of two 5-HT2 receptor subtypes in the same insect species. The results presented should facilitate further attempts to unravel central and peripheral effects of serotonin mediated by these receptors.
PLoS one
10.1371/journal.pone.0082407
1932-6203
wos:2011-2013
e82407
WOS:000328566700077
Blenau, W (reprint author), Univ Potsdam, Dept Biol & Biochem, Potsdam, Germany., Blenau@bio.uni-frankfurt.de
German Science Foundation [BL 469/7-1]
Markus Thamm
Daniel Rolke
Nadine Jordan
Sabine Balfanz
Christian Schiffer
Arnd Baumann
Wolfgang Blenau
Institut für Biochemie und Biologie
Referiert
Open Access
35049
2013
2013
eng
342
350
9
3
44
article
Springer
Paris
1
--
--
--
Suitability of three common reference genes for quantitative real-time PCR in honey bees
Honey bees are important model organisms for neurobiology, because they display a large array of behaviors. To link behavior with individual gene function, quantitative polymerase chain reaction is frequently used. Comparing gene expression of different individuals requires data normalization using adequate reference genes. These should ideally be expressed stably throughout lifetime. Unfortunately, this is frequently not the case. We studied how well three commonly used reference genes are suited for this purpose and measured gene expression in the brains of honey bees differing in age and social role. Although rpl32 is used most frequently, it only remains stable in expression between newly emerged bees, nurse-aged bees, and pollen foragers but shows a peak at the age of 12 days. The genes gapdh and ef1 alpha-f1, in contrast, are expressed stably in the brain throughout all age groups except newly emerged bees. According to stability software, gapdh was expressed most stably, followed by rpl32 and ef1 alpha-f1.
Apidologie : a quality journal in bee science
10.1007/s13592-012-0184-3
0044-8435
wos:2011-2013
WOS:000318285700011
Scheiner, R (reprint author), Univ Potsdam, Inst Biochem & Biol, Karl Liebknecht Str 24-25,Haus 26, D-14476 Potsdam, Germany., ricarda.scheiner-pietsch@uni-potsdam.de
German Research Foundation (DFG) [SCH1573/2-1, SCH1573/4-1, BL469/7-1]
Tina Reim
Markus Thamm
Daniel Rolke
Wolfgang Blenau
Ricarda Scheiner
eng
uncontrolled
gene expression
eng
uncontrolled
quantitative PCR
eng
uncontrolled
reference gene
eng
uncontrolled
stability program
eng
uncontrolled
Apis mellifera
Institut für Biochemie und Biologie
Referiert
38282
2014
2014
eng
629
653
25
1
15
review
MDPI
Basel
1
--
--
--
Characterization of an Invertebrate-Type Dopamine Receptor of the American Cockroach, Periplaneta americana
We have isolated a cDNA coding for a putative invertebrate-type dopamine receptor (Peadop2) from P. americana brain by using a PCR-based strategy. The mRNA is present in samples from brain and salivary glands. We analyzed the distribution of the PeaDOP2 receptor protein with specific affinity-purified polyclonal antibodies. On Western blots, PeaDOP2 was detected in protein samples from brain, subesophageal ganglion, thoracic ganglia, and salivary glands. In immunocytochemical experiments, we detected PeaDOP2 in neurons with their somata being located at the anterior edge of the medulla bilaterally innervating the optic lobes and projecting to the ventro-lateral protocerebrum. In order to determine the functional and pharmacological properties of the cloned receptor, we generated a cell line constitutively expressing PeaDOP2. Activation of PeaDOP2-expressing cells with dopamine induced an increase in intracellular cAMP. In contrast, a C-terminally truncated splice variant of this receptor did not exhibit any functional property by itself. The molecular and pharmacological characterization of the first dopamine receptor from P. americana provides the basis for forthcoming studies focusing on the significance of the dopaminergic system in cockroach behavior and physiology.
International journal of molecular sciences
10.3390/ijms15010629
24398985
1422-0067
wos:2014
WOS:000335776100036
Blenau, W (reprint author), Univ Potsdam, Inst Biochem & Biol, D-14476 Potsdam, Germany., b.troppmann@web.de; s.balfanz@fz-juelich.de; Christian.Krach@gmx.de; a.baumann@fz-juelich.de; Blenau@bio.uni-frankfurt.de
German Research Foundation [BL 469/4]
Britta Troppmann
Sabine Balfanz
Christian Krach
Arnd Baumann
Wolfgang Blenau
eng
uncontrolled
G-protein-coupled receptor
eng
uncontrolled
dopamine
eng
uncontrolled
insect
eng
uncontrolled
cellular signaling
eng
uncontrolled
salivary gland
eng
uncontrolled
biogenic amine
Institut für Biochemie und Biologie
Referiert
Open Access