35609
2012
2012
eng
33
41
9
87
article
Elsevier
Lausanne
1
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Human sulfite oxidase electrochemistry on gold nanoparticles modified electrode
The present study reports a facile approach for sulfite biosensing, based on enhanced direct electron transfer of a human sulfite oxidase (hSO) immobilized on a gold nanoparticles modified electrode. The spherical core shell AuNPs were prepared via a new method by reduction of HAuCl4 with branched poly(ethyleneimine) in an ionic liquids resulting particles with a diameter less than 10 nm. These nanoparticles were covalently attached to a mercaptoundecanoic acid modified Au-electrode where then hSO was adsorbed and an enhanced interfacial electron transfer and electrocatalysis was achieved. UV/Vis and resonance Raman spectroscopy, in combination with direct protein voltammetry, are employed for the characterization of the system and reveal no perturbation of the structural integrity of the redox protein. The proposed biosensor exhibited a quick steady-state current response, within 2 s, a linear detection range between 0.5 and 5.4 mu M with a high sensitivity (1.85 nA mu M-1). The investigated system provides remarkable advantages in the possibility to work at low applied potential and at very high ionic strength. Therefore these properties could make the proposed system useful in the development of bioelectronic devices and its application in real samples.
Bioelectrochemistry : an international journal devoted to electrochemical aspects of biology and biological aspects of electrochemistry ; official journal of the Bioelectrochemical Society
10.1016/j.bioelechem.2011.11.012
1567-5394
wos:2011-2013
WOS:000309033000007
Frasca, S (reprint author), Univ Potsdam, Inst Biochem & Biol, Karl Liebknecht Str 24-25,Haus 25, D-14476 Golm, Germany., uwollen@uni-potsdam.de
Cluster of Excellence "Unifying Concepts in Catalysis"; Deutsche
Forschungsgemeinschaft [EXC 314, SM 199/7-1]; ILB (TERAsens); Fonds der
Chemischen Industrie; National University of Costa Rica; National
Science Bureau of Costa Rica (MICIT); National Science Bureau of Costa
Rica (CONICIT); DAAD
Stefano Frasca
Oscar Rojas
Johannes Salewski
Bettina Neumann
Konstanze Stiba
Inez M. Weidinger
Brigitte Tiersch
Silke Leimkühler
Joachim Koetz
Ursula Wollenberger
eng
uncontrolled
Direct electron transfer
eng
uncontrolled
Gold nanoparticle
eng
uncontrolled
Human sulfite oxidase
eng
uncontrolled
Ionic liquid
eng
uncontrolled
Sulfite biosensor
Institut für Biochemie und Biologie
Referiert
34610
2013
2013
eng
172
180
9
2
110
article
PERGAMON-ELSEVIER SCIENCE LTD
OXFORD
1
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Bioelectrocatalysis at mesoporous antimony doped tin oxide
electrodes-Electrochemical characterization and direct enzyme
communication
In this paper we report immobilization and bioelectrocatalysis of human sulfite oxidase (hSO) on nanostructured antimony doped tin oxide (ATO) thin film electrodes. Two types of ATO thin film electrodes were prepared via evaporation induced self-assembly of ATO nanoparticle sols. The use of a porogen results in different porosity and film thickness. Nevertheless both electrode types reveal similar quasi reversible electrochemical behavior for positive and negatively charged small mediators. Facile and durable immobilization of catalytically active enzyme in a direct electron transfer configuration was achieved without further chemical modification of the ATO surfaces. Interestingly, the binding of hSO onto the ATO surface seems to be not only of electrostatic nature, but also originates from a strong interaction between the histidine-tag of the enzyme and the supporting material. This is suggested from stable sulfite dependent bioelectrocatalytic signals at high ionic strength and imidazole desorption experiments. As such, ATO appears as a promising conductive platform for the immobilization of complex enzymes and their application in bioelectrocatalysis. (C) 2013 Elsevier Ltd. All rights reserved.
ELECTROCHIMICA ACTA
10.1016/j.electacta.2013.03.144
0013-4686
1873-3859
wos:2011-2013
WOS:000329530300024
Fischer, A (reprint author), Tech Univ Berlin, Inst Chem, Str 17,Juni 135,Haus TK01, D-10623 Berlin, Germany.
, stefano.frasca@uni-potsdam.de; ana.i.moleromilan@campus.tu-berlin.de;
amandine@mail.tu-berlin.de; caren.goebel@tu-berlin.de;
perezf@ebv.org.ve; kstiba@uni-potsdam.de; sleim@uni-potsdam.de;
anna.fischer@tu-berlin.de; uwollen@uni-potsdam.de
Deutsche Forschungsgemeinschaft [EXC 314]; DFG [SM 199/7-1]; European
Union (EFRE) [ILB-Brandenburg 80136126]; BMBF [03IS2201B]; Cluster of
Excellence "Unifying Concepts in Catalysis"
Stefano Frasca
Anabel Molero Milan
Amandine Guiet
Caren Goebel
Fernando Perez-Caballero
Konstanze Stiba
Silke Leimkühler
Anna Fischer
Ursula Wollenberger
eng
uncontrolled
Antimony doped tin dioxide
eng
uncontrolled
Sulfite oxidase
eng
uncontrolled
Direct electrochemistry
eng
uncontrolled
Biosensor
eng
uncontrolled
Bioelectrocatalysis