38285
2014
2014
eng
3196
3203
8
21
2
article
Royal Society of Chemistry
Cambridge
1
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--
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A multilayered sulfonated polyaniline network with entrapped pyrroloquinoline quinone-dependent glucose dehydrogenase: tunable direct bioelectrocatalysis
A feasible approach to construct multilayer films of sulfonated polyanilines - PMSA1 and PABMSA1 containing different ratios of aniline, 2-methoxyaniline-5-sulfonic acid (MAS) and 3-aminobenzoic acid (AB), with the entrapped redox enzyme pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH) on Au and ITO electrode surfaces, is described. The formation of layers has been followed and confirmed by electrochemical impedance spectroscopy (EIS), which demonstrates that the multilayer assembly can be achieved in a progressive and uniform manner. The gold and ITO electrodes subsequently modified with PMSA1:PQQ-GDH and PABMSA1 films are studied by cyclic voltammetry (CV) and UV-Vis spectroscopy which show a significant direct bioelectrocatalytical response to the oxidation of the substrate glucose without any additional mediator. This response correlates linearly with the number of deposited layers. Furthermore, the constructed polymer/enzyme multilayer system exhibits a rather good long-term stability, since the catalytic current response is maintained for more than 60% of the initial value even after two weeks of storage. This verifies that a productive interaction of the enzyme embedded in the film of substituted polyaniline can be used as a basis for the construction of bioelectronic units, which are useful as indicators for processes liberating glucose and allowing optical and electrochemical transduction.
Journal of materials chemistry : B, Materials for biology and medicine
10.1039/c4tb00336e
2050-750X
2050-7518
wos:2014
WOS:000336072400004
Sarauli, D (reprint author), Tech Univ Appl Sci Wildau, Inst Appl Life Sci, Hochschulring 1, D-15745 Wildau, Germany., david.sarauli@th-wildau.de; flisdat@th-wildau.de
BMBF, Germany [02IS2201I]
David Sarauli
Chenggang Xu
Birgit Dietzel
Burkhard Schulz
Fred Lisdat
Institut für Chemie
Referiert
37470
2014
2014
eng
17887
17893
7
20
6
article
American Chemical Society
Washington
1
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3D-Electrode architectures for enhanced direct bioelectrocatalysis of pyrroloquinoline quinone-dependent glucose dehydrogenase
We report on the fabrication of a complex electrode architecture for efficient direct bioelectrocatalysis. In the developed procedure, the redox enzyme pyrroloquinoline quinone-dependent glucose dehydrogenase entrapped in a sulfonated polyaniline [poly(2-methoxyaniline-5-sulfonic acid)-co-aniline] was immobilized on macroporous indium tin oxide (macroITO) electrodes. The use of the 3D-conducting scaffold with a large surface area in combination with the conductive polymer enables immobilization of large amounts of enzyme and its efficient communication with the electrode, leading to enhanced direct bioelectrocatalysis. In the presence of glucose, the fabricated bioelectrodes show an exceptionally high direct bioelectrocatalytical response without any additional mediator. The catalytic current is increased more than 200-fold compared to planar ITO electrodes. Together with a high long-term stability (the current response is maintained for >90% of the initial value even after 2 weeks of storage), the transparent 3D macroITO structure with a conductive polymer represents a valuable basis for the construction of highly efficient bioelectronic units, which are useful as indicators for processes liberating glucose and allowing optical and electrochemical transduction.
ACS applied materials & interfaces
10.1021/am5046026
25230089
1944-8244
wos:2014
WOS:000343684200066
Sarauli, D (reprint author), Tech Univ Appl Sci Wildau, Inst Appl Life Sci, Hsch Ring 1, Wildau, Germany., david.sarauli@th-wildau.de; flisdat@th-wildau.de
BMBF, Germany [03IS2201I]; German Research Foundation (DFG) [FA 839/3-1,
SPP 1613]; NIM cluster (DFG); research network "Solar Technologies Go
Hybrid"; research network UMWELTnanoTECH (State of Bavaria); DAAD
David Sarauli
Kristina Peters
Chenggang Xu
Burkhard Schulz
Dina Fattakhova-Rohlfing
Fred Lisdat
eng
uncontrolled
3D electrode structures
eng
uncontrolled
macroITO
eng
uncontrolled
conductive polymer
eng
uncontrolled
PQQ-GDH
eng
uncontrolled
direct bioelectrocatalysis
eng
uncontrolled
bioelectrochemistry
Institut für Chemie
Referiert
37340
2014
2014
eng
2437
2445
9
24
215
article
Wiley-VCH
Weinheim
1
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Characterization of Langmuir films prepared from copolyesterurethanes based on oligo(omega-pentadecalactone) and oligo(epsilon-caprolactone)segments
A series of multiblock copolymers (PDLCL) synthesized from oligo(omega-pentadecalactone) diol (OPDL) and oligo(epsilon-caprolactone) diol (OCL), which are linked by 2,2(4), 4-trimethyl-hexamethylene diisocyanate (TMDI), is investigated by the Langmuir monolayer technique at the air-water interface. Brewster angle microscopy (BAM) and spectroscopic ellipsometry are employed to characterize the polymer film morphologies in situ. PDLCL containing >= 40 wt% OCL segments form homogeneous Langmuir monofilms after spreading. The film elasticity modulus decreases with increasing amounts of OPDL segments in the copolymer. In contrast, the OCL-free polyesterurethane OPDL-TMDI cannot be spread to monomolecular films on the water surface properly, and movable slabs are observed by BAM even at low surface pressures. The results of the in situ morphological characterization clearly show that essential information concerning the reliability of Langmuir monolayer degradation (LMD) experiments cannot be obtained from the evaluation of the pi-A isotherms only. Consequently, in situ morphological characterization turns out to be indispensable for characterization of Langmuir layers before LMD experiments.
Macromolecular chemistry and physics
10.1002/macp.201400377
1022-1352
1521-3935
wos:2014
WOS:000346268200006
Lendlein, A (reprint author), Helmholtz Zentrum Geesthacht, Inst Biomat Sci, Kantstr 55, D-14513 Teltow, Germany., andreas.lendlein@hzg.de
Anne-Christin Schöne
Burkhard Schulz
Klaus Richau
Karl Kratz
Andreas Lendlein
eng
uncontrolled
brewster angle microscopy
eng
uncontrolled
ellipsometry
eng
uncontrolled
Langmuir layers
eng
uncontrolled
morphology
eng
uncontrolled
polyesterurethanes
Institut für Chemie
Referiert
9874
2014
eng
postprint
1
--
--
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A multilayered sulfonated polyaniline network with entrapped pyrroloquinoline quinone-dependent glucose dehydrogenase
A feasible approach to construct multilayer films of sulfonated polyanilines – PMSA1 and PABMSA1 – containing different ratios of aniline, 2-methoxyaniline-5-sulfonic acid (MAS) and 3-aminobenzoic acid (AB), with the entrapped redox enzyme pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH) on Au and ITO electrode surfaces, is described. The formation of layers has been followed and confirmed by electrochemical impedance spectroscopy (EIS), which demonstrates that the multilayer assembly can be achieved in a progressive and uniform manner. The gold and ITO electrodes subsequently modified with PMSA1:PQQ-GDH and PABMSA1 films are studied by cyclic voltammetry (CV) and UV-Vis spectroscopy which show a significant direct bioelectrocatalytical response to the oxidation of the substrate glucose without any additional mediator. This response correlates linearly with the number of deposited layers. Furthermore, the constructed polymer/enzyme multilayer system exhibits a rather good long-term stability, since the catalytic current response is maintained for more than 60% of the initial value even after two weeks of storage. This verifies that a productive interaction of the enzyme embedded in the film of substituted polyaniline can be used as a basis for the construction of bioelectronic units, which are useful as indicators for processes liberating glucose and allowing optical and electrochemical transduction.
tunable direct bioelectrocatalysis
urn:nbn:de:kobv:517-opus4-98744
online registration
J. Mater. Chem. B, (2014) Nr. 2, S. 3196-3203. - DOI: 10.1039/C4TB00336E
Keine öffentliche Lizenz: Unter Urheberrechtsschutz
David Sarauli
Chenggang Xu
Birgit Dietzel
Burkhard Schulz
Fred Lisdat
Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
275
Chemie und zugeordnete Wissenschaften
Biowissenschaften; Biologie
Medizin und Gesundheit
open_access
Institut für Chemie
Referiert
Open Access
Universität Potsdam
https://publishup.uni-potsdam.de/files/9874/pmnr275_online.pdf