43565
2019
2019
eng
14
745
postprint
1
2019-10-02
2019-10-02
--
A high‑throughput amplicon‑based method for estimating outcrossing rates
Background: The outcrossing rate is a key determinant of the population-genetic structure of species and their long-term evolutionary trajectories. However, determining the outcrossing rate using current methods based on PCRgenotyping individual offspring of focal plants for multiple polymorphic markers is laborious and time-consuming.
Results: We have developed an amplicon-based, high-throughput enabled method for estimating the outcrossing rate and have applied this to an example of scented versus non-scented Capsella (Shepherd’s Purse) genotypes. Our results show that the method is able to robustly capture differences in outcrossing rates. They also highlight potential biases in the estimates resulting from differential haplotype sharing of the focal plants with the pollen-donor population at individual amplicons.
Conclusions: This novel method for estimating outcrossing rates will allow determining this key population-genetic parameter with high-throughput across many genotypes in a population, enabling studies into the genetic determinants of successful pollinator attraction and outcrossing.
Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe
10.25932/publishup-43565
urn:nbn:de:kobv:517-opus4-435657
1866-8372
Plant Methods 15 (2019) 47 DOI: 10.1186/s13007-019-0433-9
<a href="http://publishup.uni-potsdam.de/opus4-ubp/frontdoor/index/index/docId/43564">Bibliographieeintrag der Originalveröffentlichung/Quelle</a>
false
true
CC-BY - Namensnennung 4.0 International
Friederike Jantzen
Natalia Joanna Wozniak
Christian Kappel
Adrien Sicard
Michael Lenhard
Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
745
eng
uncontrolled
Outcrossing
eng
uncontrolled
Mixed mating
eng
uncontrolled
Outcrossing rate
eng
uncontrolled
Capsella
eng
uncontrolled
Amplicon sequencing
Biowissenschaften; Biologie
Pflanzen (Botanik)
open_access
Institut für Biochemie und Biologie
Referiert
Open Access
Universität Potsdam
https://publishup.uni-potsdam.de/files/43565/pmnr745.pdf
43564
2019
2019
eng
14
47
15
article
BioMed Central
London
1
2019-05-18
2019-05-18
--
A high‑throughput amplicon‑based method for estimating outcrossing rates
Background: The outcrossing rate is a key determinant of the population-genetic structure of species and their long-term evolutionary trajectories. However, determining the outcrossing rate using current methods based on PCRgenotyping individual offspring of focal plants for multiple polymorphic markers is laborious and time-consuming.
Results: We have developed an amplicon-based, high-throughput enabled method for estimating the outcrossing rate and have applied this to an example of scented versus non-scented Capsella (Shepherd’s Purse) genotypes. Our results show that the method is able to robustly capture differences in outcrossing rates. They also highlight potential biases in the estimates resulting from differential haplotype sharing of the focal plants with the pollen-donor population at individual amplicons.
Conclusions: This novel method for estimating outcrossing rates will allow determining this key population-genetic parameter with high-throughput across many genotypes in a population, enabling studies into the genetic determinants of successful pollinator attraction and outcrossing.
Plant Methods
10.1186/s13007-019-0433-9
1746-4811
Universität Potsdam
PA 2019_44
1894.48
<a href="http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-435657">Zweitveröffentlichung in der Schriftenreihe Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe ; 745</a>
false
false
CC-BY - Namensnennung 4.0 International
Friederike Jantzen
Natalia Joanna Wozniak
Christian Kappel
Adrien Sicard
Michael Lenhard
eng
uncontrolled
Outcrossing
eng
uncontrolled
Mixed mating
eng
uncontrolled
Outcrossing rate
eng
uncontrolled
Capsella
eng
uncontrolled
Amplicon sequencing
Biowissenschaften; Biologie
Pflanzen (Botanik)
open_access
Institut für Biochemie und Biologie
Referiert
Publikationsfonds der Universität Potsdam
Open Access