38452
2015
2015
eng
419
425
7
174
article
Elsevier
Clare
1
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Antiplasmodial and cytotoxic activities of the constituents of Turraea robusta and Turraea nilotica
Ethnopharmacological relevance: Turraea robusta and Turraea nilotica are African medicinal plants used for the treatment of a wide variety of diseases, including malaria. The genus Turraea is rich in limonoids and other triterpenoids known to possess various biological activities.
Materials and methods: From the stem bark of T. robusta six compounds, and from various parts of T nilotica eleven compounds were isolated by the use of a combination of chromatographic techniques. The structures of the isolated compounds were elucidated using NMR and MS, whilst the relative configuration of one of the isolated compounds, toonapubesin F, was established by X-ray crystallography. The antiplasmodial activities of the crude extracts and the isolated constituents against the D6 and W2 strains of Plasmodium falciparum were determined using the semiautomated micro dilution technique that measures the ability of the extracts to inhibit the incorporation of (G-H-3, where G is guanine) hypoxanthine into the malaria parasite. The cytotoxicity of the crude extracts and their isolated constituents was evaluated against the mammalian cell lines African monkey kidney (vero), mouse breast cancer (4T1) and human larynx carcinoma (HEp2).
Results: The extracts showed good to moderate antiplasmodial activities, where the extract of the stem bark of T. robusta was also cytotoxic against the 4T1 and the HEp2 cells (IC50 < 10 mu g/ml). The compounds isolated from these extracts were characterized as limonoids, protolimonoids and phytosterol glucosides. These compounds showed good to moderate activities with the most active one being azadironolide, IC50 2.4 +/- 0.03 mu M and 1.1 +/- 0.01 mu M against the D6 and W2 strains of Plasmodium falciparum, respectively; all other compounds possessed IC50 14.4-40.5 mu M. None of the compounds showed significant cytotoxicity against vero cells, yet four of them were toxic against the 4T1 and HEp2 cancer cell lines with piscidinol A having IC50 8.0 +/- 0.03 and 8.4 +/- 0.01 mu M against the 4T1 and HEp2 cells, respectively. Diacetylation of piscidinol A resulted in reduced cytotoxicity.
Conclusion: From the medicinal plants T. robusta and T. nilotica, twelve compounds were isolated and characterized; two of the isolated compounds, namely 11-epi-toonacilin and azadironolide showed good antiplasmodial activity with the highest selectivity indices. (C) 2015 The Authors. Published by Elsevier Ireland Ltd.
Journal of ethnopharmacology : an interdisciplinary journal devoted to bioscientific research on indigenous drugs
10.1016/j.jep.2015.08.039
26320684
0378-8741
wos:2015
WOS:000365050900044
Yenesew, A (reprint author), Univ Nairobi, Dept Chem, POB 30197-00100, Nairobi, Kenya., ayenesew@uonbi.ac.ke; mate@chem.gu.se
IFS [F/4575-2]; UNICEF/UNDP/WORLD BANK/WHO Special Programme for
Research and Training in Tropical Diseases [SPHQ10-RTG-6 ID: A90208];
Swedish Research Council [2012-6124]
Beatrice N. Irungu
Nicholas Adipo
Jennifer A. Orwa
Francis Kimani
Matthias Heydenreich
Jacob O. Midiwo
Per Martin Bjoremark
Mikael Hakansson
Abiy Yenesew
Mate Erdelyi
eng
uncontrolled
Turraea robusta
eng
uncontrolled
Turraea nilotica
eng
uncontrolled
Antiplasmodial activity
eng
uncontrolled
Cytotoxicity
eng
uncontrolled
Limonoid
eng
uncontrolled
Toonapubesins F
eng
uncontrolled
Toonacilin
eng
uncontrolled
Azadironolide
Institut für Chemie
Referiert
35228
2013
2013
eng
175
176
2
2
8
article
NPC
Westerville
1
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Antiplasmodial activity of compounds from the surface exudates of senecio roseiflorus
From the surface exudates of Senecio roseiflorus fourteen known methylated flavonoids and one phenol were isolated and characterized. The structures of these compounds were determined on the basis of their spectroscopic analysis. The surface exudate and the flavonoids isolated showed moderate to good antiplasmodial activity with 5,4'-dihydroxy-7-dimethoxyflavanone having the highest activity against chloroquine-sensitive (D6) and resistant (W2) strains of Plasmodium falciparum, with IC50 values of 3.2 +/- 0.8 and 4.4 +/- 0.01 mu g/mL respectively.
Natural product communications : an international journal for communications and reviews
1934-578X
wos:2011-2013
WOS:000315369000010
Langat, MK (reprint author), Univ Surrey, FEPS, Dept Chem, Guildford GU2 7XH, Surrey, England., m.langat@surrey.ac.uk
German Academic Exchange Service (DAAD); Deutsche
Forschungsgemeinschaft, Germany [Pe 264/ 14-5]; Bundesministerium fur
Zusammenarbeit [Pe-254/14-6]
Leonidah Omosa Kerubo
Jacob Ogweno Midiwo
Solomon Derese
Moses K. Langat
Hoseah M. Akala
Norman C. Waters
Martin Peter
Matthias Heydenreich
eng
uncontrolled
Senecio roseiflorus
eng
uncontrolled
Asteraceae
eng
uncontrolled
Surface exudates
eng
uncontrolled
Antiplasmodial activity
Institut für Chemie
Referiert