44696
2016
2016
eng
1051
1063
13
52
article
Wiley-Blackwell
Hoboken
1
--
--
--
ECOPHYSIOLOGY MATTERS: LINKING INORGANIC CARBON ACQUISITION TO ECOLOGICAL PREFERENCE IN FOUR SPECIES OF MICROALGAE (CHLOROPHYCEAE)
The effect of CO2 supply is likely to play an important role in algal ecology. Since inorganic carbon (C-i) acquisition strategies are very diverse among microalgae and C-i availability varies greatly within and among habitats, we hypothesized that C-i acquisition depends on the pH of their preferred natural environment (adaptation) and that the efficiency of C-i uptake is affected by CO2 availability (acclimation). To test this, four species of green algae originating from different habitats were studied. The pH-drift and C-i uptake kinetic experiments were used to characterize C-i acquisition strategies and their ability to acclimate to high and low CO2 conditions and high and low pH was evaluated. Results from pH drift experiments revealed that the acidophile and acidotolerant Chlamydomonas species were mainly restricted to CO2, whereas the two neutrophiles were efficient bicarbonate users. CO2 compensation points in low CO2-acclimated cultures ranged between 0.6 and 1.4 mu M CO2 and acclimation to different culture pH and CO2 conditions suggested that CO2 concentrating mechanisms were present in most species. High CO2 acclimated cultures adapted rapidly to low CO2 condition during pH-drifts. C-i uptake kinetics at different pH values showed that the affinity for C-i was largely influenced by external pH, being highest under conditions where CO2 dominated the C-i pool. In conclusion, C-i acquisition was highly variable among four species of green algae and linked to growth pH preference, suggesting that there is a connection between C-i acquisition and ecological distribution.
Journal of phycology
10.1111/jpy.12462
27624741
0022-3646
1529-8817
wos2016:2019
WOS:000390344600013
Lachmann, SC (reprint author), Univ Potsdam, Neuen Palais 10, D-14469 Potsdam, Germany., sabrina.lachmann@uni-potsdam.de
German Research Foundation [SP695/5-1]; UK Natural Environment Research Council
importub
2020-03-22T12:20:01+00:00
filename=package.tar
67fd4ff7faf2e4dabd3ee219e93b778a
Sabrina C. Lachmann
Stephen C. Maberly
Elly Spijkerman
eng
uncontrolled
acidophile
eng
uncontrolled
carbon acquisition
eng
uncontrolled
CCM
eng
uncontrolled
Chlamydomonas
eng
uncontrolled
Chlorella
eng
uncontrolled
CO2 supply
eng
uncontrolled
extremophile
eng
uncontrolled
inorganic carbon uptake kinetics
eng
uncontrolled
pH-drift
eng
uncontrolled
Scenedesmus
Institut für Biochemie und Biologie
Referiert
Import
36249
2012
2012
eng
138
145
8
2
47
article
Routledge, Taylor & Francis Group
Abingdon
1
--
--
--
Phytoplankton sterol contents vary with temperature, phosphorus and silicate supply a study on three freshwater species
The understanding of environmentally induced changes in the biochemical composition of phytoplankton species is of great importance in both physiological studies and ecological food web research. In extensive laboratory experiments we tested the influence of two different temperatures (10 degrees C and 25 degrees C) and a phosphorus supply gradient on the sterol concentrations of the three freshwater phytoplankton species Scenedesmus quadricauda, Cryptomonas ovata and Cyclotella meneghiniana. The diatom C. meneghiniana was additionally exposed to a silicate gradient. In two separate experiments we analysed (1) possible interactive effects of temperature and phosphorus supply and (2) the effect of four phosphorus levels and three silicate levels on algal sterol concentrations. We observed that sterol concentrations were higher at 25 degrees C than at 10 degrees C in S. quadricauda and C. meneghiniana, but were not affected by temperature in C. ovata. Interactive effects of temperature and phosphorus supply on sterol concentrations were found in C. meneghiniana. This presumably was due to the bioconversion of one sterol (24-methylenecholesterol) into another (22-dihydrobrassicasterol). Increasing phosphorus supply resulted in species-specific effects on sterol concentrations, viz. an optimum curve response in S. quadricauda, a saturation curve response in C. meneghiniana and no change in sterol concentration in C. ovata. Effects of silicate supply on the sterols of C. meneghiniana equalled the effects of phosphorus supply. Albeit we did not observe a general trend in the three phytoplankton species tested, we conclude that sterol concentrations of phytoplankton are strongly affected by temperature and nutrient supply. Interactive effects point out the importance of taking into account more than just one environmental factor when assessing the effects of environmentally induced changes on phytoplankton sterol concentrations.
European journal of phycology
10.1080/09670262.2012.665484
0967-0262
wos:2011-2013
WOS:000310900100001
Piepho, M (reprint author), Univ Potsdam, Inst Biochem & Biol, Neuen Palais 10, D-14469 Potsdam, Germany., piepho@uni-potsdam.de
German Research Foundation [DFG WA 2445/4-1]
Maike Piepho
Dominik Martin-Creuzburg
Alexander Wacker
eng
uncontrolled
algae
eng
uncontrolled
Chlorophyta
eng
uncontrolled
Cryptomonas
eng
uncontrolled
Cryptophyta
eng
uncontrolled
Cyclotella
eng
uncontrolled
diatoms
eng
uncontrolled
phosphorus
eng
uncontrolled
Scenedesmus
eng
uncontrolled
silicate
eng
uncontrolled
sterols
eng
uncontrolled
temperature
Institut für Biochemie und Biologie
Referiert
36170
2012
2012
eng
64
73
10
1
48
article
Wiley-Blackwell
Malden
1
--
--
--
Species-specific variation in fatty acid concentrations of four phytoplankton species does phosphorus supply influence the effect of light intensity of temperature?
We tested, in the laboratory, the influence of light intensity, temperature, and phosphorus (P) supply on fatty acid (FA) concentrations of four freshwater algae: the green algae Scenedesmus quadricauda (Turpin) Breb. and Chlamydomonas globosa J. Snow, the cryptophyte Cryptomonas ovata Ehrenb., and the diatom Cyclotella meneghiniana Kutz. We investigated the main and interactive effects of two variables on algal FA concentrations (i.e., light intensity and P supply or temperature and P supply). Interactive effects of light intensity and P supply were most pronounced in C. meneghiniana, but were also found in S. quadricauda and C. ovata. Changes in several saturated and unsaturated FA concentrations with light were more distinct in the low-P treatments than in the high-P treatments. Interactive effects of temperature and P supply on various FA concentrations were observed in all four species, but there was no consistent pattern. In lake ecosystems, P limitation often coincides with high light intensities and temperatures in summer. Therefore, it is important to examine how combinations of these environmental conditions affect FA concentrations of primary producers that are important sources of FAs for higher trophic levels.
Journal of phycology
10.1111/j.1529-8817.2011.01103.x
0022-3646
wos:2011-2013
WOS:000299730600006
Piepho, M (reprint author), Univ Potsdam, Inst Biochem & Biol, Neuen Palais 10, D-14469 Potsdam, Germany., piepho@uni-potsdam.de
German Research Foundation (DFG) [WA 2445/4-1]; Environment Canada
Maike Piepho
Michael T. Arts
Alexander Wacker
eng
uncontrolled
Chlamydomonas
eng
uncontrolled
Cryptomonas
eng
uncontrolled
Cyclotella
eng
uncontrolled
fatty acids
eng
uncontrolled
light
eng
uncontrolled
lipids
eng
uncontrolled
phosphate
eng
uncontrolled
PUFA
eng
uncontrolled
Scenedesmus
eng
uncontrolled
temperature
Institut für Biochemie und Biologie
Referiert