44509
2020
2020
eng
26
823
postprint
1
2020-02-26
2020-02-26
--
A Biostimulant Obtained from the Seaweed Ascophyllum nodosum Protects Arabidopsis thaliana from Severe Oxidative Stress
Abiotic stresses cause oxidative damage in plants. Here, we demonstrate that foliar application of an extract from the seaweed Ascophyllum nodosum, SuperFifty (SF), largely prevents paraquat (PQ)-induced oxidative stress in Arabidopsis thaliana. While PQ-stressed plants develop necrotic lesions, plants pre-treated with SF (i.e., primed plants) were unaffected by PQ. Transcriptome analysis revealed induction of reactive oxygen species (ROS) marker genes, genes involved in ROS-induced programmed cell death, and autophagy-related genes after PQ treatment. These changes did not occur in PQ-stressed plants primed with SF. In contrast, upregulation of several carbohydrate metabolism genes, growth, and hormone signaling as well as antioxidant-related genes were specific to SF-primed plants. Metabolomic analyses revealed accumulation of the stress-protective metabolite maltose and the tricarboxylic acid cycle intermediates fumarate and malate in SF-primed plants. Lipidome analysis indicated that those lipids associated with oxidative stress-induced cell death and chloroplast degradation, such as triacylglycerols (TAGs), declined upon SF priming. Our study demonstrated that SF confers tolerance to PQ-induced oxidative stress in A. thaliana, an effect achieved by modulating a range of processes at the transcriptomic, metabolic, and lipid levels.
Postprints der Universität Potsdam : Mathematisch Naturwissenschaftliche Reihe
10.25932/publishup-44509
urn:nbn:de:kobv:517-opus4-445093
1866-8372
International Journal of Molecular Sciences 21 (2020) 2 Art. 474 DOI: 10.3390/ijms21020474
<a href="http://publishup.uni-potsdam.de/44508">Bibliographieeintrag der Originalveröffentlichung/Quelle</a>
474
CC-BY - Namensnennung 4.0 International
Mohammad Amin Omidbakhshfard
Sujeeth Neerakkal
Saurabh Gupta
Nooshin Omranian
Kieran J. Guinan
Yariv Brotman
Zoran Nikoloski
Alisdair R. Fernie
Bernd Mueller-Roeber
Tsanko S. Gechev
Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
823
eng
uncontrolled
Ascophyllum nodosum
eng
uncontrolled
Arabidopsis thaliana
eng
uncontrolled
biostimulant
eng
uncontrolled
paraquat
eng
uncontrolled
priming
eng
uncontrolled
oxidative stress tolerance
eng
uncontrolled
reactive oxygen species
Chemie und zugeordnete Wissenschaften
Biowissenschaften; Biologie
open_access
Mathematisch-Naturwissenschaftliche Fakultät
Referiert
Open Access
Universität Potsdam
https://publishup.uni-potsdam.de/files/44509/pmnr823.pdf
44508
2020
2019
eng
26
2
21
article
Molecular Diversity Preservation International
Basel
1
2020-01-09
2019-12-05
--
A Biostimulant Obtained from the Seaweed Ascophyllum nodosum Protects Arabidopsis thaliana from Severe Oxidative Stress
Abiotic stresses cause oxidative damage in plants. Here, we demonstrate that foliar application of an extract from the seaweed Ascophyllum nodosum, SuperFifty (SF), largely prevents paraquat (PQ)-induced oxidative stress in Arabidopsis thaliana. While PQ-stressed plants develop necrotic lesions, plants pre-treated with SF (i.e., primed plants) were unaffected by PQ. Transcriptome analysis revealed induction of reactive oxygen species (ROS) marker genes, genes involved in ROS-induced programmed cell death, and autophagy-related genes after PQ treatment. These changes did not occur in PQ-stressed plants primed with SF. In contrast, upregulation of several carbohydrate metabolism genes, growth, and hormone signaling as well as antioxidant-related genes were specific to SF-primed plants. Metabolomic analyses revealed accumulation of the stress-protective metabolite maltose and the tricarboxylic acid cycle intermediates fumarate and malate in SF-primed plants. Lipidome analysis indicated that those lipids associated with oxidative stress-induced cell death and chloroplast degradation, such as triacylglycerols (TAGs), declined upon SF priming. Our study demonstrated that SF confers tolerance to PQ-induced oxidative stress in A. thaliana, an effect achieved by modulating a range of processes at the transcriptomic, metabolic, and lipid levels.
International Journal of Molecular Sciences
10.3390/ijms21020474
1422-0067
Universität Potsdam
PA 2020_009
1243.0
<a href="https://doi.org/10.25932/publishup-44509">Zweitveröffentlichung in der Schriftenreihe Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe ; 823</a>
474
CC-BY - Namensnennung 4.0 International
Mohammad Amin Omidbakhshfard
Sujeeth Neerakkal
Saurabh Gupta
Nooshin Omranian
Kieran J. Guinan
Yariv Brotman
Zoran Nikoloski
Alisdair R. Fernie
Bernd Mueller-Roeber
Tsanko S. Gechev
eng
uncontrolled
Ascophyllum nodosum
eng
uncontrolled
Arabidopsis thaliana
eng
uncontrolled
biostimulant
eng
uncontrolled
paraquat
eng
uncontrolled
priming
eng
uncontrolled
oxidative stress tolerance
eng
uncontrolled
reactive oxygen species
Chemie und zugeordnete Wissenschaften
Biowissenschaften; Biologie
Mathematisch-Naturwissenschaftliche Fakultät
Referiert
Publikationsfonds der Universität Potsdam
Open Access