Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Abteilungen OPUS4-37357 Wissenschaftlicher Artikel Ghylin, Trevor W.; Garcia, Sarahi L.; Moya, Francisco; Oyserman, Ben O.; Schwientek, Patrick; Forest, Katrina T.; Mutschler, James; Dwulit-Smith, Jeffrey; Chan, Leong-Keat; Martinez-Garcia, Manuel; Sczyrba, Alexander; Stepanauskas, Ramunas; Grossart, Hans-Peter; Woyke, Tanja; Warnecke, Falk; Malmstrom, Rex; Bertilsson, Stefan; McMahon, Katherine D. Comparative single-cell genomics reveals potential ecological niches for the freshwater acl Actinobacteria lineage Members of the acI lineage of Actinobacteria are the most abundant microorganisms in most freshwater lakes; however, our understanding of the keys to their success and their role in carbon and nutrient cycling in freshwater systems has been hampered by the lack of pure cultures and genomes. We obtained draft genome assemblies from 11 single cells representing three acI tribes (acI-A1, acI-A7, acI-B1) from four temperate lakes in the United States and Europe. Comparative analysis of acI SAGs and other available freshwater bacterial genomes showed that acI has more gene content directed toward carbohydrate acquisition as compared to Polynucleobacter and LD12 Alphaproteobacteria, which seem to specialize more on carboxylic acids. The acI genomes contain actinorhodopsin as well as some genes involved in anaplerotic carbon fixation indicating the capacity to supplement their known heterotrophic lifestyle. Genome-level differences between the acI-A and acI-B clades suggest specialization at the clade level for carbon substrate acquisition. Overall, the acI genomes appear to be highly streamlined versions of Actinobacteria that include some genes allowing it to take advantage of sunlight and N-rich organic compounds such as polyamines, di-and oligopeptides, branched-chain amino acids and cyanophycin. This work significantly expands the known metabolic potential of the cosmopolitan freshwater acI lineage and its ecological and genetic traits. London Nature Publ. Group 2014 14 The ISME journal : multidisciplinary journal of microbial ecology 8 12 2503 2516 10.1038/ismej.2014.135 Institut für Geowissenschaften OPUS4-42729 misc Garcia, Sarahi L.; Buck, Moritz; Hamilton, Joshua J.; Wurzbacher, Christian; Grossart, Hans-Peter; McMahon, Katherine D.; Eiler, Alexander Model communities hint at promiscuous metabolic linkages between ubiquitous free-living freshwater bacteria Genome streamlining is frequently observed in free-living aquatic microorganisms and results in physiological dependencies between microorganisms. However, we know little about the specificity of these microbial associations. In order to examine the specificity and extent of these associations, we established mixed cultures from three different freshwater environments and analyzed the cooccurrence of organisms using a metagenomic time series. Free-living microorganisms with streamlined genomes lacking multiple biosynthetic pathways showed no clear recurring pattern in their interaction partners. Free-living freshwater bacteria form promiscuous cooperative associations. This notion contrasts with the well-documented high specificities of interaction partners in host-associated bacteria. Considering all data together, we suggest that highly abundant free-living bacterial lineages are functionally versatile in their interactions despite their distinct streamlining tendencies at the single-cell level. This metabolic versatility facilitates interactions with a variable set of community members. 2018 8 Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe 714 urn:nbn:de:kobv:517-opus4-427299 10.25932/publishup-42729 Mathematisch-Naturwissenschaftliche Fakultät OPUS4-38105 Wissenschaftlicher Artikel Salka, Ivette; Wurzbacher, Christian; Garcia, Sarahi L.; Labrenz, Matthias; Juergens, Klaus; Grossart, Hans-Peter Distribution of acI-Actinorhodopsin genes in Baltic Sea salinity gradients indicates adaptation of facultative freshwater photoheterotrophs to brackish waters Hoboken Wiley-Blackwell 2014 12 Environmental microbiology 16 2 586 597 Institut für Biochemie und Biologie OPUS4-38119 Wissenschaftlicher Artikel Garcia, Sarahi L.; McMahon, Katherine D.; Grossart, Hans-Peter; Warnecke, Falk Successful enrichment of the ubiquitous freshwater acI Actinobacteria Actinobacteria of the acI lineage are often the numerically dominant bacterial phylum in surface freshwaters, where they can account for >50% of total bacteria. Despite their abundance, there are no described isolates. In an effort to obtain enrichment of these ubiquitous freshwater Actinobacteria, diluted freshwater samples from Lake Grosse Fuchskuhle, Germany, were incubated in 96-well culture plates. With this method, a successful enrichment containing high abundances of a member of the lineage acI was established. Phylogenetic classification showed that the acIActinobacteria of the enrichment belonged to the acI-B2 tribe, which seems to prefer acidic lakes. This enrichment grows to low cell densities and thus the oligotrophic nature of acI-B2 was confirmed. Hoboken Wiley-Blackwell 2014 7 Environmental microbiology reports 6 1 21 27 10.1111/1758-2229.12104 Institut für Biochemie und Biologie OPUS4-38649 Wissenschaftlicher Artikel Garcia, Sarahi L.; Buck, Moritz; McMahon, Katherine D.; Grossart, Hans-Peter; Eiler, Alexander; Warnecke, Falk Auxotrophy and intrapopulation complementary in the "interactome' of a cultivated freshwater model community Microorganisms are usually studied either in highly complex natural communities or in isolation as monoclonal model populations that we manage to grow in the laboratory. Here, we uncover the biology of some of the most common and yet-uncultured bacteria in freshwater environments using a mixed culture from Lake Grosse Fuchskuhle. From a single shotgun metagenome of a freshwater mixed culture of low complexity, we recovered four high-quality metagenome-assembled genomes (MAGs) for metabolic reconstruction. This analysis revealed the metabolic interconnectedness and niche partitioning of these naturally dominant bacteria. In particular, vitamin- and amino acid biosynthetic pathways were distributed unequally with a member of Crenarchaeota most likely being the sole producer of vitamin B12 in the mixed culture. Using coverage-based partitioning of the genes recovered from a single MAG intrapopulation metabolic complementarity was revealed pointing to social' interactions for the common good of populations dominating freshwater plankton. As such, our MAGs highlight the power of mixed cultures to extract naturally occurring interactomes' and to overcome our inability to isolate and grow the microbes dominating in nature. Hoboken Wiley-Blackwell 2015 11 Molecular ecology 24 17 4449 4459 10.1111/mec.13319 Institut für Biochemie und Biologie OPUS4-52943 Wissenschaftlicher Artikel Garcia, Sarahi L.; Buck, Moritz; Hamilton, Joshua J.; Wurzbacher, Christian; Grossart, Hans-Peter; McMahon, Katherine D.; Eiler, Alexander Model communities hint at promiscuous metabolic linkages between ubiquitous free-living freshwater bacteria Genome streamlining is frequently observed in free-living aquatic microorganisms and results in physiological dependencies between microorganisms. However, we know little about the specificity of these microbial associations. In order to examine the specificity and extent of these associations, we established mixed cultures from three different freshwater environments and analyzed the cooccurrence of organisms using a metagenomic time series. Free-living microorganisms with streamlined genomes lacking multiple biosynthetic pathways showed no clear recurring pattern in their interaction partners. Free-living freshwater bacteria form promiscuous cooperative associations. This notion contrasts with the well-documented high specificities of interaction partners in host-associated bacteria. Considering all data together, we suggest that highly abundant free-living bacterial lineages are functionally versatile in their interactions despite their distinct streamlining tendencies at the single-cell level. This metabolic versatility facilitates interactions with a variable set of community members. Washington American Society for Microbiology 2018 8 mSphere 3 3 10.1128/mSphere.00202-18 Institut für Biochemie und Biologie