Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Abteilungen OPUS4-42729 misc Garcia, Sarahi L.; Buck, Moritz; Hamilton, Joshua J.; Wurzbacher, Christian; Grossart, Hans-Peter; McMahon, Katherine D.; Eiler, Alexander Model communities hint at promiscuous metabolic linkages between ubiquitous free-living freshwater bacteria Genome streamlining is frequently observed in free-living aquatic microorganisms and results in physiological dependencies between microorganisms. However, we know little about the specificity of these microbial associations. In order to examine the specificity and extent of these associations, we established mixed cultures from three different freshwater environments and analyzed the cooccurrence of organisms using a metagenomic time series. Free-living microorganisms with streamlined genomes lacking multiple biosynthetic pathways showed no clear recurring pattern in their interaction partners. Free-living freshwater bacteria form promiscuous cooperative associations. This notion contrasts with the well-documented high specificities of interaction partners in host-associated bacteria. Considering all data together, we suggest that highly abundant free-living bacterial lineages are functionally versatile in their interactions despite their distinct streamlining tendencies at the single-cell level. This metabolic versatility facilitates interactions with a variable set of community members. 2018 8 Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe 714 urn:nbn:de:kobv:517-opus4-427299 10.25932/publishup-42729 Mathematisch-Naturwissenschaftliche Fakultät OPUS4-38649 Wissenschaftlicher Artikel Garcia, Sarahi L.; Buck, Moritz; McMahon, Katherine D.; Grossart, Hans-Peter; Eiler, Alexander; Warnecke, Falk Auxotrophy and intrapopulation complementary in the "interactome' of a cultivated freshwater model community Microorganisms are usually studied either in highly complex natural communities or in isolation as monoclonal model populations that we manage to grow in the laboratory. Here, we uncover the biology of some of the most common and yet-uncultured bacteria in freshwater environments using a mixed culture from Lake Grosse Fuchskuhle. From a single shotgun metagenome of a freshwater mixed culture of low complexity, we recovered four high-quality metagenome-assembled genomes (MAGs) for metabolic reconstruction. This analysis revealed the metabolic interconnectedness and niche partitioning of these naturally dominant bacteria. In particular, vitamin- and amino acid biosynthetic pathways were distributed unequally with a member of Crenarchaeota most likely being the sole producer of vitamin B12 in the mixed culture. Using coverage-based partitioning of the genes recovered from a single MAG intrapopulation metabolic complementarity was revealed pointing to social' interactions for the common good of populations dominating freshwater plankton. As such, our MAGs highlight the power of mixed cultures to extract naturally occurring interactomes' and to overcome our inability to isolate and grow the microbes dominating in nature. Hoboken Wiley-Blackwell 2015 11 Molecular ecology 24 17 4449 4459 10.1111/mec.13319 Institut für Biochemie und Biologie OPUS4-52943 Wissenschaftlicher Artikel Garcia, Sarahi L.; Buck, Moritz; Hamilton, Joshua J.; Wurzbacher, Christian; Grossart, Hans-Peter; McMahon, Katherine D.; Eiler, Alexander Model communities hint at promiscuous metabolic linkages between ubiquitous free-living freshwater bacteria Genome streamlining is frequently observed in free-living aquatic microorganisms and results in physiological dependencies between microorganisms. However, we know little about the specificity of these microbial associations. In order to examine the specificity and extent of these associations, we established mixed cultures from three different freshwater environments and analyzed the cooccurrence of organisms using a metagenomic time series. Free-living microorganisms with streamlined genomes lacking multiple biosynthetic pathways showed no clear recurring pattern in their interaction partners. Free-living freshwater bacteria form promiscuous cooperative associations. This notion contrasts with the well-documented high specificities of interaction partners in host-associated bacteria. Considering all data together, we suggest that highly abundant free-living bacterial lineages are functionally versatile in their interactions despite their distinct streamlining tendencies at the single-cell level. This metabolic versatility facilitates interactions with a variable set of community members. Washington American Society for Microbiology 2018 8 mSphere 3 3 10.1128/mSphere.00202-18 Institut für Biochemie und Biologie