Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Abteilungen OPUS4-43565 misc Jantzen, Friederike; Wozniak, Natalia Joanna; Kappel, Christian; Sicard, Adrien; Lenhard, Michael A high‑throughput amplicon‑based method for estimating outcrossing rates Background: The outcrossing rate is a key determinant of the population-genetic structure of species and their long-term evolutionary trajectories. However, determining the outcrossing rate using current methods based on PCRgenotyping individual offspring of focal plants for multiple polymorphic markers is laborious and time-consuming. Results: We have developed an amplicon-based, high-throughput enabled method for estimating the outcrossing rate and have applied this to an example of scented versus non-scented Capsella (Shepherd's Purse) genotypes. Our results show that the method is able to robustly capture differences in outcrossing rates. They also highlight potential biases in the estimates resulting from differential haplotype sharing of the focal plants with the pollen-donor population at individual amplicons. Conclusions: This novel method for estimating outcrossing rates will allow determining this key population-genetic parameter with high-throughput across many genotypes in a population, enabling studies into the genetic determinants of successful pollinator attraction and outcrossing. 2019 14 Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe 745 urn:nbn:de:kobv:517-opus4-435657 10.25932/publishup-43565 Institut für Biochemie und Biologie OPUS4-43754 misc Jantzen, Friederike; Lynch, Joseph H.; Kappel, Christian; Höfflin, Jona; Skaliter, Oded; Wozniak, Natalia Joanna; Sicard, Adrien; Sas, Claudia; Adebesin, Funmilayo; Ravid, Jasmin; Vainstein, Alexander; Hilker, Monika; Dudareva, Natalia; Lenhard, Michael Retracing the molecular basis and evolutionary history of the loss of benzaldehyde emission in the genus Capsella The transition from pollinator-mediated outbreeding to selfing has occurred many times in angiosperms. This is generally accompanied by a reduction in traits attracting pollinators, including reduced emission of floral scent. In Capsella, emission of benzaldehyde as a main component of floral scent has been lost in selfing C. rubella by mutation of cinnamate-CoA ligase CNL1. However, the biochemical basis and evolutionary history of this loss remain unknown, as does the reason for the absence of benzaldehyde emission in the independently derived selfer Capsella orientalis. We used plant transformation, in vitro enzyme assays, population genetics and quantitative genetics to address these questions. CNL1 has been inactivated twice independently by point mutations in C. rubella, causing a loss of enzymatic activity. Both inactive haplotypes are found within and outside of Greece, the centre of origin of C. rubella, indicating that they arose before its geographical spread. By contrast, the loss of benzaldehyde emission in C. orientalis is not due to an inactivating mutation in CNL1. CNL1 represents a hotspot for mutations that eliminate benzaldehyde emission, potentially reflecting the limited pleiotropy and large effect of its inactivation. Nevertheless, even closely related species have followed different evolutionary routes in reducing floral scent. 2019 12 Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe 775 1349 1360 urn:nbn:de:kobv:517-opus4-437542 10.25932/publishup-43754 Mathematisch-Naturwissenschaftliche Fakultät