Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Abteilungen OPUS4-38771 Konferenzveröffentlichung Haralampiev, Ivan; Mertens, Monique; Schwarzer, Roland; Herrmann, Andreas; Volkmer, Rudolf; Wessig, Pablo; Müller, Peter A palmitic acid functionalized with a maleimide group is used to recruit SH-containing peptides to lipid and biological membranes Hoboken Wiley-Blackwell 2015 1 The FEBS journal 282 204 204 Institut für Chemie OPUS4-35660 Wissenschaftlicher Artikel Wawrzinek, Robert; Wessig, Pablo; Möllnitz, Kristian; Nikolaus, Joerg; Schwarzer, Roland; Müller, Peter; Herrmann, Andreas DBD dyes as fluorescent probes for sensing lipophilic environments Small fluorescent organic molecules based on [1,3]dioxolo[4,5-f][1,3]benzodioxole (DBD) could be used as probes for lipophillic microenvironments in aqueous solutions by indicating the critical micelles concentration of detergents and staining cell organelles. Their fluorescence lifetime decreases drastically by the amount of water in their direct environment. Therefore they are potential probes for fluorescence lifetime imaging microscopy (FLIM). Oxford Elsevier 2012 5 Bioorganic & medicinal chemistry letters : a Tetrahedron publication for rapid dissemination of preliminary communications on all aspects of bioorganic chemistry, medicinal chemistry and related disciplines 22 17 5367 5371 10.1016/j.bmcl.2012.07.056 Institut für Chemie OPUS4-31814 Wissenschaftlicher Artikel Müller, Peter; Nikolaus, Jörg; Schiller, Sabine; Herrmann, Andreas; Moellnitz, Kristian; Czapla, Sylvia; Wessig, Pablo Molecular rods with oligospiroketal backbones as anchors in biological membranes Getting stuck in: A hydrophobic molecular rod with terminal fluorescent moieties has been synthesized. The insertion of the rod into membranes was investigated and shown to incorporate efficiently into model and biological membranes (see picture; gray C, blue N, red O). Those rods can be used as stable membrane-associated anchors for functionalization of membrane surfaces. 2009 10.1002/anie.200901133 Institut für Chemie OPUS4-36486 Wissenschaftlicher Artikel Nikolaus, Jörg; Czapla, Sylvia; Möllnitz, Kristian; Höfer, Chris T.; Herrmann, Andreas; Wessig, Pablo; Müller, Peter New molecular rods - Characterization of their interaction with membranes Molecular rods are synthetical molecules consisting of a hydrophobic backbone which are functionalized with varying terminal groups. Here, we report on the interaction of a recently described new class of molecular rods with lipid and biological membranes. In order to characterize this interaction, different fluorescently labeled rods were synthesized allowing for the application of fluorescence spectroscopy and microscopy based approaches. Our data show that the rods are incorporated into membranes with a perpendicular orientation to the membrane surface and enrich preferentially in liquid-disordered lipid domains. These characteristics underline that rods can be applied as stable membrane-associated anchors for functionalizing membrane surfaces. Amsterdam Elsevier 2011 8 Biochimica et biophysica acta : Biomembranes 1808 12 2781 2788 10.1016/j.bbamem.2011.08.008 Institut für Chemie OPUS4-35234 Wissenschaftlicher Artikel Grimm, Christiane; Meyer, Thomas; Czapla, Sylvia; Nikolaus, Jörg; Scheidt, Holger A.; Vogel, Alexander; Herrmann, Andreas; Wessig, Pablo; Huster, Daniel; Müller, Peter Structure and dynamics of molecular rods in membranes application of a Spin-Labeled rod Molecular rods consisting of a hydrophobic backbone and terminally varying functional groups have been synthesized for applications for the functionalization of membranes. In the present study, we employ a spin-labeled analogue of a recently described new class of molecular rods to characterize their dynamic interactions with membranes. By using the different approaches of ESR and NMR spectroscopy, we show that the spin moiety of the membrane-embedded spin-labeled rod is localized in the upper chain/glycerol region of membranes of different compositions. The rod is embedded within the membrane in a tilted orientation to adjust for the varying hydrophobic thicknesses of these bilayers. This orientation does not perturb the membrane structure. The water solubility of the rod is increased significantly in the presence of certain cyclodextrins. These cyclodextrins also allow the rods to be extracted from the membrane and incorporated into preformed membranes. The latter will improve the future applications of these rods in cellular systems as stable membrane-associated anchors for the functionalization of membrane surfaces. Weinheim Wiley-VCH 2013 8 Chemistry - a European journal 19 8 2703 2710 10.1002/chem.201202500 Institut für Chemie OPUS4-52270 Wissenschaftlicher Artikel Mertens, Monique; Hilsch, Malte; Haralampiev, Ivan; Volkmer, Rudolf; Wessig, Pablo; Müller, Peter Synthesis and characterization of a new Bifunctionalized, Fluorescent, and Amphiphilic molecule for recruiting SH-Containing molecules to membranes This study describes the synthesis and characterization of an amphiphilic construct intended to recruit SH-containing molecules to membranes. The construct consists of 1)an aliphatic chain to enable anchoring within membranes, 2)a maleimide moiety to react with the sulfhydryl group of a soluble (bio)molecule, and 3)a fluorescence moiety to allow the construct to be followed by fluorescence spectroscopy and microscopy. It is shown that the construct can be incorporated into preformed membranes, thus allowing application of the approach with biological membranes. The close proximity between the fluorophore and the maleimide moiety within the construct causes fluorescence quenching. This allows monitoring of the reaction with SH-containing molecules by measurement of increases in fluorescence intensity and lifetime. Notably, the construct distributes into laterally ordered membrane domains of lipid vesicles, which is probably triggered by the length of its membrane anchor. The advantages of the new construct can be employed for several biological, biotechnological, and medicinal applications. Weinheim Wiley-VCH 2018 5 ChemBioChem 19 15 1643 1647 10.1002/cbic.201800268 Institut für Chemie