Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Abteilungen OPUS4-45972 misc Dvornikov, Yury; Leibman, Marina; Heim, Birgit; Bartsch, Annett; Herzschuh, Ulrike; Skorospekhova, Tatiana; Fedorova, Irina; Khomutov, Artem; Widhalm, Barbara; Gubarkov, Anatoly; Rößler, Sebastian Terrestrial CDOM in lakes of Yamal Peninsula In this study, we analyze interactions in lake and lake catchment systems of a continuous permafrost area. We assessed colored dissolved organic matter (CDOM) absorption at 440 nm (a(440)(CDOM)) and absorption slope (S300-500) in lakes using field sampling and optical remote sensing data for an area of 350 km(2) in Central Yamal, Siberia. Applying a CDOM algorithm (ratio of green and red band reflectance) for two high spatial resolution multispectral GeoEye-1 and Worldview-2 satellite images, we were able to extrapolate the a()(CDOM) data from 18 lakes sampled in the field to 356 lakes in the study area (model R-2 = 0.79). Values of a(440)(CDOM) in 356 lakes varied from 0.48 to 8.35 m(-1) with a median of 1.43 m(-1). This a()(CDOM) dataset was used to relate lake CDOM to 17 lake and lake catchment parameters derived from optical and radar remote sensing data and from digital elevation model analysis in order to establish the parameters controlling CDOM in lakes on the Yamal Peninsula. Regression tree model and boosted regression tree analysis showed that the activity of cryogenic processes (thermocirques) in the lake shores and lake water level were the two most important controls, explaining 48.4% and 28.4% of lake CDOM, respectively (R-2 = 0.61). Activation of thermocirques led to a large input of terrestrial organic matter and sediments from catchments and thawed permafrost to lakes (n = 15, mean a(440)(CDOM) = 5.3 m(-1)). Large lakes on the floodplain with a connection to Mordy-Yakha River received more CDOM (n = 7, mean a(440)(CDOM) = 3.8 m(-1)) compared to lakes located on higher terraces. 2018 21 Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe 1333 urn:nbn:de:kobv:517-opus4-459720 10.25932/publishup-45972 Institut für Geowissenschaften OPUS4-42709 misc Olmer, Ruth; Engels, Lena; Usman, Abdulai; Menke, Sandra; Malik, Muhammad Nasir Hayat; Pessler, Frank; Göhring, Gudrun; Bornhorst, Dorothee; Bolten, Svenja; Abdelilah-Seyfried, Salim; Scheper, Thomas; Kempf, Henning; Zweigerdt, Robert; Martin, Ulrich Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture Endothelial cells (ECs) are involved in a variety of cellular responses. As multifunctional components of vascular structures, endothelial (progenitor) cells have been utilized in cellular therapies and are required as an important cellular component of engineered tissue constructs and in vitro disease models. Although primary ECs from different sources are readily isolated and expanded, cell quantity and quality in terms of functionality and karyotype stability is limited. ECs derived from human induced pluripotent stem cells (hiPSCs) represent an alternative and potentially superior cell source, but traditional culture approaches and 2D differentiation protocols hardly allow for production of large cell numbers. Aiming at the production of ECs, we have developed a robust approach for efficient endothelial differentiation of hiPSCs in scalable suspension culture. The established protocol results in relevant numbers of ECs for regenerative approaches and industrial applications that show in vitro proliferation capacity and a high degree of chromosomal stability. 2018 18 Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe 5 urn:nbn:de:kobv:517-opus4-427095 10.25932/publishup-42709 Institut für Biochemie und Biologie OPUS4-45916 misc Pauly, Maren; Helle, Gerhard; Miramont, Cécile; Büntgen, Ulf; Treydte, Kerstin; Reinig, Frederick; Guibal, Frédéric; Sivan, Olivier; Heinrich, Ingo; Riedel, Frank; Kromer, Bernd; Balanzategui, Daniel; Wacker, Lukas; Sookdeo, Adam Sookdeo; Brauer, Achim Subfossil trees suggest enhanced Mediterranean hydroclimate variability at the onset of the Younger Dryas Nearly 13,000 years ago, the warming trend into the Holocene was sharply interrupted by a reversal to near glacial conditions. Climatic causes and ecological consequences of the Younger Dryas (YD) have been extensively studied, however proxy archives from the Mediterranean basin capturing this period are scarce and do not provide annual resolution. Here, we report a hydroclimatic reconstruction from stable isotopes (delta O-18, delta C-13) in subfossil pines from southern France. Growing before and during the transition period into the YD (12 900-12 600 cal BP), the trees provide an annually resolved, continuous sequence of atmospheric change. Isotopic signature of tree sourcewater (delta O-18(sw)) and estimates of relative air humidity were reconstructed as a proxy for variations in air mass origin and precipitation regime. We find a distinct increase in inter-annual variability of sourcewater isotopes (delta O-18(sw)), with three major downturn phases of increasing magnitude beginning at 12 740 cal BP. The observed variation most likely results from an amplified intensity of North Atlantic (low delta O-18(sw)) versus Mediterranean (high delta O-18(sw)) precipitation. This marked pattern of climate variability is not seen in records from higher latitudes and is likely a consequence of atmospheric circulation oscillations at the margin of the southward moving polar front. 2018 10 Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe 1135 urn:nbn:de:kobv:517-opus4-459169 10.25932/publishup-45916 Mathematisch-Naturwissenschaftliche Fakultät OPUS4-44638 misc Manzoni, Stefano; Čapek, Petr; Porada, Philipp; Thurner, Martin; Winterdahl, Mattias; Beer, Christian; Brüchert, Volker; Frouz, Jan; Herrmann, Anke M.; Lindahl, Björn D.; Lyon, Steve W.; Šantrůčková, Hana; Vico, Giulia; Way, Danielle Reviews and syntheses The cycling of carbon (C) between the Earth surface and the atmosphere is controlled by biological and abiotic processes that regulate C storage in biogeochemical compartments and release to the atmosphere. This partitioning is quantified using various forms of C-use efficiency (CUE) - the ratio of C remaining in a system to C entering that system. Biological CUE is the fraction of C taken up allocated to biosynthesis. In soils and sediments, C storage depends also on abiotic processes, so the term C-storage efficiency (CSE) can be used. Here we first review and reconcile CUE and CSE definitions proposed for autotrophic and heterotrophic organisms and communities, food webs, whole ecosystems and watersheds, and soils and sediments using a common mathematical framework. Second, we identify general CUE patterns; for example, the actual CUE increases with improving growth conditions, and apparent CUE decreases with increasing turnover. We then synthesize > 5000CUE estimates showing that CUE decreases with increasing biological and ecological organization - from uni-cellular to multicellular organisms and from individuals to ecosystems. We conclude that CUE is an emergent property of coupled biological-abiotic systems, and it should be regarded as a flexible and scale-dependent index of the capacity of a given system to effectively retain C. 2018 23 Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe 1134 urn:nbn:de:kobv:517-opus4-446386 10.25932/publishup-44638 Mathematisch-Naturwissenschaftliche Fakultät OPUS4-44606 misc Tabben, Montassar; Ihsan, Mohammed; Ghoul, Nihel; Coquart, Jeremy; Chaouachi, Anis; Chaabene, Helmi; Tourny, Claire; Chamari, Karim Cold Water Immersion Enhanced Athletes' Wellness and 10-m Short Sprint Performance 24-h After a Simulated Mixed Martial Arts Combat Objective: The aim of the present study was to examine the effect of Cold Water Immersion (CWI) on the recovery of physical performance, hematological stress markers and perceived wellness (i.e., Hooper scores) following a simulated Mixed Martial Arts (MMA) competition. Methods: Participants completed two experimental sessions in a counter-balanced order (CWI or passive recovery for control condition: CON), after a simulated MMAs competition (3 x 5-min MMA rounds separated by 1-min of passive rest). During CWI, athletes were required to submerge their bodies, except the trunk, neck and head, in the seated position in a temperature-controlled bath (similar to 10 degrees C) for 15-min. During CON, athletes were required to be in a seated position for 15-min in same room ambient temperature. Venous blood samples (creatine kinase, cortisol, and testosterone concentrations) were collected at rest (PRE-EX, i.e., before MMAs), immediately following MMAs (POST-EX), immediately following recovery (POST-R) and 24 h post MMAs (POST-24), whilst physical fitness (squat jump, countermovement-jump and 5- and 10-m sprints) and perceptual measures (well-being Hooper index: fatigue, stress, delayed onset muscle soreness (DOMS), and sleep) were collected at PRE-EX, POST-R and POST-24, and at PRE-EX and POST-24, respectively. Results: The main results indicate that POST-R sprint (5- and 10-m) performances were 'likely to very likely' (d = 0.64 and 0.65) impaired by prior CWI. However, moderate improvements were in 10-m sprint performance were 'likely' evident at POST-24 after CWI compared with CON (d = 0.53). Additionally, the use of CWI 'almost certainly' resulted in a large overall improvement in Hooper scores (d = 1.93). Specifically, CWI 'almost certainly' resulted in improved sleep quality (d = 1.36), stress (d = 1.56) and perceived fatigue (d = 1.51), and 'likely' resulted in a moderate decrease in DOMS (d = 0.60). Conclusion: The use of CWI resulted in an enhanced recovery of 10-m sprint performance, as well as improved perceived wellness 24-h following simulated MMA competition. 2018 10 Postprints der Universität Potsdam : Humanwissenschaftliche Reihe 707 urn:nbn:de:kobv:517-opus4-446068 10.25932/publishup-44606 Humanwissenschaftliche Fakultät OPUS4-45911 misc Odongo, Grace Akinyi; Schlotz, Nina; Baldermann, Susanne; Neugart, Susanne; Huyskens-Keil, Susanne; Ngwene, Benard; Trierweiler, Bernhard; Schreiner, Monika; Lamy, Evelyn African nightshade (Solanum scabrum Mill.) Plant cultivation and processing may impact nutrient and phytochemical content of vegetables. The present study aimed at determining the influence of cultivation and processing on the health promoting capacity of African nightshade (Solanum scabrum Mill.) leaves, an indigenous vegetable, rich in nutrients and phytochemicals. Anti-genotoxicity against the human liver carcinogen aflatoxin B1 (AFB1) as determined by the comet assay and radical oxygen species (ROS) scavenging capacity of ethanolic and aqueous extracts were investigated in human derived liver (HepG2) cells. ROS scavenging activity was assessed using electron paramagnetic spin resonance and quantification of ARE/Nrf2 mediated gene expression. The cultivation was done under different environmental conditions. The processing included fermentation and cooking; postharvest ultraviolet irradiation (UV-C) treatment was also investigated. Overall, S. scabrum extracts showed strong health promoting potential, the highest potential was observed with the fermented extract, which showed a 60% reduction of AFB1 induced DNA damage and a 38% reduction in FeSO4 induced oxidative stress. The content of total polyphenols, carotenoids and chlorophylls was indeed affected by cultivation and processing. Based on the present in vitro findings consumption of S. scabrum leaves could be further encouraged, preferentially after cooking or fermentation of the plant. 2018 22 Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe 1133 urn:nbn:de:kobv:517-opus4-459114 10.25932/publishup-45911 Mathematisch-Naturwissenschaftliche Fakultät OPUS4-46869 misc Krupkova, Olga; Sadowska, Aleksandra; Kameda, Takuya; Hitzl, Wolfgang; Hausmann, Oliver Nic; Klasen, Jürgen; Wuertz-Kozak, Karin p38 MaPK facilitates crosstalk between endoplasmic reticulum stress and IL-6 release in the intervertebral Disc Degenerative disc disease is associated with increased expression of pro-inflammatory cytokines in the intervertebral disc (IVD). However, it is not completely clear how inflammation arises in the IVD and which cellular compartments are involved in this process. Recently, the endoplasmic reticulum (ER) has emerged as a possible modulator of inflammation in age-related disorders. In addition, ER stress has been associated with the microenvironment of degenerated IVDs. Therefore, the aim of this study was to analyze the effects of ER stress on inflammatory responses in degenerated human IVDs and associated molecular mechanisms. Gene expression of ER stress marker GRP78 and pro-inflammatory cytokines IL-6, IL-8, IL-1 beta, and TNF-alpha was analyzed in human surgical IVD samples (n = 51, Pfirrmann grade 2-5). The expression of GRP78 positively correlated with the degeneration grade in lumbar IVDs and IL-6, but not with IL-1 beta and TNF-alpha. Another set of human surgical IVD samples (n = 25) was used to prepare primary cell cultures. ER stress inducer thapsigargin (Tg, 100 and 500 nM) activated gene and protein expression of IL-6 and induced phosphorylation of p38 MAPK. Both inhibition of p38 MAPK by SB203580 (10 mu M) and knockdown of ER stress effector CCAAT-enhancer-binding protein homologous protein (CHOP) reduced gene and protein expression of IL-6 in Tg-treated cells. Furthermore, the effects of an inflammatory microenvironment on ER stress were tested. TNF-alpha (5 and 10 ng/mL) did not activate ER stress, while IL-1 beta (5 and 10 ng/mL) activated gene and protein expression of GRP78, but did not influence [Ca2+](i) flux and expression of CHOP, indicating that pro-inflammatory cytokines alone may not induce ER stress in vivo. This study showed that IL-6 release in the IVD can be initiated following ER stress and that ER stress mediates IL-6 release through p38 MAPK and CHOP. Therapeutic targeting of ER stress response may reduce the consequences of the harsh microenvironment in degenerated IVD. 2018 16 Postprints der Universität Potsdam : Humanwissenschaftliche Reihe 705 urn:nbn:de:kobv:517-opus4-468698 10.25932/publishup-46869 Department Sport- und Gesundheitswissenschaften OPUS4-45970 misc Reindl, Nicole; Finch, Nicolle L.; Schaffenroth, Veronika; Barstow, Martin A.; Casewell, Sarah L.; Geier, Stephan Alfred; Bertolami Miller, Marcelo Miguel; Taubenberger, Stefan Revealing the true nature of Hen2-428 The nucleus of Hen 2-428 is a short orbital period (4.2 h) spectroscopic binary, whose status as potential supernovae type Ia progenitor has raised some controversy in the literature. We present preliminary results of a thorough analysis of this interesting system, which combines quantitative non-local thermodynamic (non-LTE) equilibrium spectral modelling, radial velocity analysis, multi-band light curve fitting, and state-of-the art stellar evolutionary calculations. Importantly, we find that the dynamical system mass that is derived by using all available He II lines does not exceed the Chandrasekhar mass limit. Furthermore, the individual masses of the two central stars are too small to lead to an SN Ia in case of a dynamical explosion during the merger process. 2018 9 Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe 1129 urn:nbn:de:kobv:517-opus4-459703 10.25932/publishup-45970 Mathematisch-Naturwissenschaftliche Fakultät OPUS4-45985 misc König, Johanna; Block, Andrea; Becker, Matthias; Fenske, Kristin; Hertel, Johannes; Van der Auwera, Sandra; Zymara, Kathleen; Völzke, Henry; Freyberger, Harald Jürgen; Grabe, Hans Jörgen Assessment of subjective emotional valence and long-lasting impact of life events Background: Life events (LEs) are associated with future physical and mental health. They are crucial for understanding the pathways to mental disorders as well as the interactions with biological parameters. However, deeper insight is needed into the complex interplay between the type of LE, its subjective evaluation and accompanying factors such as social support. The "Stralsund Life Event List" (SEL) was developed to facilitate this research. Methods: The SEL is a standardized interview that assesses the time of occurrence and frequency of 81 LEs, their subjective emotional valence, the perceived social support during the LE experience and the impact of past LEs on present life. Data from 2265 subjects from the general population-based cohort study "Study of Health in Pomerania" (SHIP) were analysed. Based on the mean emotional valence ratings of the whole sample, LEs were categorized as "positive" or "negative". For verification, the SEL was related to lifetime major depressive disorder (MDD; Munich Composite International Diagnostic Interview), childhood trauma (Childhood Trauma Questionnaire), resilience (Resilience Scale) and subjective health (SF-12 Health Survey). Results: The report of lifetime MDD was associated with more negative emotional valence ratings of negative LEs (OR = 2.96, p < 0.0001). Negative LEs (b = 0.071, p < 0.0001, beta = 0.25) and more negative emotional valence ratings of positive LEs (b = 3.74, p < 0.0001, beta = 0.11) were positively associated with childhood trauma. In contrast, more positive emotional valence ratings of positive LEs were associated with higher resilience (b = -7.05, p < 0.0001, beta = 0.13), and a lower present impact of past negative LEs was associated with better subjective health (b = 2.79, p = 0.001, beta = 0.05). The internal consistency of the generated scores varied considerably, but the mean value was acceptable (averaged Cronbach's alpha > 0.75). Conclusions: The SEL is a valid instrument that enables the analysis of the number and frequency of LEs, their emotional valence, perceived social support and current impact on life on a global score and on an individual item level. Thus, we can recommend its use in research settings that require the assessment and analysis of the relationship between the occurrence and subjective evaluation of LEs as well as the complex balance between distressing and stabilizing life experiences. 2018 14 Postprints der Universität Potsdam : Humanwissenschaftliche Reihe 704 urn:nbn:de:kobv:517-opus4-459856 10.25932/publishup-45985 Humanwissenschaftliche Fakultät OPUS4-45965 misc Hodgkins, Suzanne B.; Richardson, Curtis J.; Dommain, René; Wang, Hongjun; Glaser, Paul H.; Verbeke, Brittany; Winkler, B. Rose; Cobb, Alexander R.; Rich, Virginia I.; Missilmani, Malak; Flanagan, Neal; Ho, Mengchi; Hoyt, Alison M.; Harvey, Charles F.; Vining, S. Rose; Hough, Moira A.; Moore, Tim R.; Richard, Pierre J. H.; De La Cruz, Florentino B.; Toufaily, Joumana; Hamdan, Rasha; Cooper, William T.; Chanton, Jeffrey P. Tropical peatland carbon storage linked to global latitudinal trends in peat recalcitrance Peatlands represent large terrestrial carbon banks. Given that most peat accumulates in boreal regions, where low temperatures and water saturation preserve organic matter, the existence of peat in (sub)tropical regions remains enigmatic. Here we examined peat and plant chemistry across a latitudinal transect from the Arctic to the tropics. Near-surface low-latitude peat has lower carbohydrate and greater aromatic content than near-surface high-latitude peat, creating a reduced oxidation state and resulting recalcitrance. This recalcitrance allows peat to persist in the (sub)tropics despite warm temperatures. Because we observed similar declines in carbohydrate content with depth in high-latitude peat, our data explain recent field-scale deep peat warming experiments in which catotelm (deeper) peat remained stable despite temperature increases up to 9 degrees C. We suggest that high-latitude deep peat reservoirs may be stabilized in the face of climate change by their ultimately lower carbohydrate and higher aromatic composition, similar to tropical peats. 2018 15 Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe 1125 urn:nbn:de:kobv:517-opus4-459658 10.25932/publishup-45965 Mathematisch-Naturwissenschaftliche Fakultät