@article{SchwiebsThomasKleuseretal.2017, author = {Schwiebs, Anja and Thomas, Dominique Jeanette and Kleuser, Burkhard and Pfeilschifter, Josef and Radeke, Heinfried H.}, title = {Nuclear translocation of SGPP-1 and decrease of SGPL-1 activity contribute to sphingolipid rheostat regulation of inflammatory dendritic cells}, series = {Mediators of inflammation}, journal = {Mediators of inflammation}, publisher = {Hindawi Publishing Corp.}, address = {London}, issn = {0962-9351}, doi = {10.1155/2017/5187368}, pages = {10}, year = {2017}, abstract = {A balanced sphingolipid rheostat is indispensable for dendritic cell function and survival and thus initiation of an immune response. Sphingolipid levels are dynamically maintained by the action of sphingolipid enzymes of which sphingosine kinases, S1P phosphatases (SGPP-1/2) and S1P lyase (SGPL-1), are pivotal in the balance of S1P and sphingosine levels. In this study, we present that SGPP-1 and SGPL-1 are regulated in inflammatory dendritic cells and contribute to S1P fate. TLR-dependent activation caused SGPL-1 protein downregulation with subsequent decrease of enzymatic activity by two-thirds. In parallel, confocal fluorescence microscopy revealed that endogenous SGPP-1 was expressed in nuclei of naive dendritic cells and was translocated into the cytoplasmatic compartment upon inflammatory stimulation resulting in dephosphorylation of S1P. Mass spectrometric determination showed that a part of the resulting sphingosine was released from the cell, increasing extracellular levels. Another route of diminishing intracellular S1P was possibly taken by its export via ATP-binding cassette transporter C1 which was upregulated in array analysis, while the S1P transporter, spinster homolog 2, was not relevant in dendritic cells. These investigations newly describe the sequential expression and localization of the endogenous S1P regulators SGPP-1 and SGPL-1 and highlight their contribution to the sphingolipid rheostat in inflammation.}, language = {en} } @article{WestburyDalerumbNorenetal.2017, author = {Westbury, Michael V. and Dalerumb, Fredrik and Noren, Karin and Hofreiter, Michael}, title = {Complete mitochondrial genome of a bat-eared fox (Otocyon megalotis), along with phylogenetic considerations}, series = {Mitochondrial DNA. Part B}, volume = {2}, journal = {Mitochondrial DNA. Part B}, number = {1}, publisher = {Routledge, Taylor \& Francis Group}, address = {London}, issn = {2380-2359}, doi = {10.1080/23802359.2017.1331325}, pages = {298 -- 299}, year = {2017}, abstract = {The bat-eared fox, Otocyon megalotis, is the only member of its genus and is thought to occupy a basal position within the dog family. These factors can lead to challenges in complete mitochondrial reconstructions and accurate phylogenetic positioning. Here, we present the first complete mitochondrial genome of the bat-eared fox recovered using shotgun sequencing and iterative mapping to three distantly related species. Phylogenetic analyses placed the bat-eared fox basal in the Canidae family within the clade including true foxes (Vulpes) and the raccoon dog (Nyctereutes) with high support values. This position is in good agreement with previously published results based on short fragments of mitochondrial and nuclear genes, therefore adding more support to the basal positioning of the bat-eared fox within Canidae.}, language = {en} } @article{OPUS4-56760, title = {Plant Hormones}, series = {Methods in Molecular Biology}, journal = {Methods in Molecular Biology}, number = {1497}, editor = {Kleine-Vehn, J{\"u}rgen and Sauer, Michael}, publisher = {Springer}, address = {New York}, isbn = {978-1-4939-6467-3}, issn = {1064-3745}, doi = {10.1007/978-1-4939-6469-7}, pages = {XI, 288}, year = {2017}, abstract = {This volume aims to present a representative cross-section of modern experimental approaches relevant to Plant Hormone Biology, ranging from relatively simple physiological to highly sophisticated methods. Chapters describe physiological, developmental, microscopy-based techniques, measure hormone contents, and heterologous systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.}, language = {en} } @misc{KleineVehnSauer2017, author = {Kleine-Vehn, J{\"u}rgen and Sauer, Michael}, title = {Preface}, series = {Plant Hormones: Methods and Protocols}, volume = {1497}, journal = {Plant Hormones: Methods and Protocols}, editor = {Kleine-Vehn, J{\"u}rgen and Sauer, Michael}, edition = {3}, publisher = {Springer}, address = {New York}, isbn = {978-1-4939-6469-7}, issn = {1064-3745}, doi = {10.1007/978-1-4939-6469-7}, pages = {V -- V}, year = {2017}, language = {en} } @phdthesis{Janowski2017, author = {Janowski, Marcin Andrzej}, title = {Investigating role of the essential GTPase - AtRsgA in the assembly of the small ribosomal subunit in Arabidopsis thaliana chloroplast}, school = {Universit{\"a}t Potsdam}, pages = {114}, year = {2017}, language = {en} } @article{HenkelColemanSchraplauetal.2017, author = {Henkel, Janin and Coleman, Charles Dominic and Schraplau, Anne and J{\"o}hrens, Korinna and Weber, Daniela and Castro, Jose Pedro and Hugo, Martin and Schulz, Tim Julius and Kr{\"a}mer, Stephanie and Sch{\"u}rmann, Annette and P{\"u}schel, Gerhard Paul}, title = {Induction of Steatohepatitis (NASH) with Insulin Resistance in Wild-type B6 Mice by a Western-type Diet Containing Soybean Oil and Cholesterol}, series = {Molecular medicine}, volume = {23}, journal = {Molecular medicine}, publisher = {Feinstein Inst. for Medical Research}, address = {Manhasset}, issn = {1076-1551}, doi = {10.2119/molmed.2016.00203}, pages = {70 -- 82}, year = {2017}, abstract = {Nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH) are hepatic manifestations of the metabolic syndrome. Many currently used animal models of NAFLD/NASH lack clinical features of either NASH or metabolic syndrome such as hepatic inflammation and fibrosis (e.g., high-fat diets) or overweight and insulin resistance (e.g., methionine-choline-deficient diets), or they are based on monogenetic defects (e.g., ob/ob mice). In the current study, a Western-type diet containing soybean oil with high n-6-PUFA and 0.75\% cholesterol (SOD + Cho) induced steatosis, inflammation and fibrosis accompanied by hepatic lipid peroxidation and oxidative stress in livers of C57BL/6-mice, which in addition showed increased weight gain and insulin resistance, thus displaying a phenotype closely resembling all clinical features of NASH in patients with metabolic syndrome. In striking contrast, a soybean oil-containing Western-type diet without cholesterol (SOD) induced only mild steatosis but not hepatic inflammation, fibrosis, weight gain or insulin resistance. Another high-fat diet, mainly consisting of lard and supplemented with fructose in drinking water (LAD + Fru), resulted in more prominent weight gain, insulin resistance and hepatic steatosis than SOD + Cho, but livers were devoid of inflammation and fibrosis. Although both LAD + Fru-and SOD + Cho-fed animals had high plasma cholesterol, liver cholesterol was elevated only in SOD + Cho animals. Cholesterol induced expression of chemotactic and inflammatory cytokines in cultured Kupffer cells and rendered hepatocytes more susceptible to apoptosis. In summary, dietary cholesterol in the SOD + Cho diet may trigger hepatic inflammation and fibrosis. SOD + Cho-fed animals may be a useful disease model displaying many clinical features of patients with the metabolic syndrome and NASH.}, language = {en} } @article{BergholzMayGiladietal.2017, author = {Bergholz, Kolja and May, Felix and Giladi, Itamar and Ristow, Michael and Ziv, Yaron and Jeltsch, Florian}, title = {Environmental heterogeneity drives fine-scale species assembly and functional diversity of annual plants in a semi-arid environment}, series = {Perspectives in plant ecology, evolution and systematics}, volume = {24}, journal = {Perspectives in plant ecology, evolution and systematics}, publisher = {Elsevier}, address = {Jena}, issn = {1433-8319}, doi = {10.1016/j.ppees.2017.01.001}, pages = {138 -- 146}, year = {2017}, abstract = {Spatial environmental heterogeneity is considered a fundamental factor for the maintenance of plant species richness. However, it still remains unclear whether heterogeneity may also facilitate coexistence at fine grain sizes or whether other processes, like mass effects and source sink dynamics due to dispersal, control species composition and diversity at these scales. In this study, we used two complimentary analyses to identify the role of heterogeneity within 15 m x 15 m plots for the coexistence of species-rich annual communities in a semi-arid environment along a steep precipitation gradient. Specifically, we: (a) analyzed the effect of environmental heterogeneity on species, functional and phylogenetic diversity within microsites (alpha diversity, 0.06 m(2) and 1 m(2)), across microsites (beta diversity), and diversity at the entire plot (gamma diversity); (b) further we used two null models to detect non-random trait and phylogenetic patterns in order to infer assembly processes, i.e. whether co-occurring species tend to share similar traits (trait convergence) or dissimilar traits (trait divergence). In general, our results showed that heterogeneity had a positive effect on community diversity. Specifically, for alpha diversity, the effect was significant for functional diversity, and not significant for either species or phylogenetic diversities. For beta diversity, all three measures of community diversity (species, functional, and phylogenetic) increased significantly, as they also did for gamma diversity, where functional measures were again stronger than for species or phylogenetic measures. In addition, the null model approach consistently detected trait convergence, indicating that species with similar traits tended to co-occur and had high abundances in a given microsite. While null model analysis across the phylogeny partly supported these trait findings, showing phylogenetic underdispersion at the 1m(2) grain size, surprisingly when species abundances in microsites were analyzed they were more evenly distributed across the phylogenetic tress than expected (phylogenetic overdispersion). In conclusion, our results provide compelling support that environmental heterogeneity at a relatively fine scale is an important factor for species co-existence as it positively affects diversity as well as influences species assembly. Our study underlines the need for trait-based approaches conducted at fine grain sizes in order to better understand species coexistence and community assembly. (C) 2017 Elsevier GmbH. All rights reserved.}, language = {en} } @phdthesis{Knecht2017, author = {Knecht, Volker}, title = {Modeling Biomolecular Association}, school = {Universit{\"a}t Potsdam}, pages = {297}, year = {2017}, language = {en} } @article{SpijkermanLukasWacker2017, author = {Spijkerman, Elly and Lukas, Marcus and Wacker, Alexander}, title = {Ecophysiological strategies for growth under varying light and organic carbon supply in two species of green microalgae differing in their motility}, series = {Phytochemistry : an international journal of plant biochemistry}, volume = {144}, journal = {Phytochemistry : an international journal of plant biochemistry}, publisher = {Elsevier}, address = {Oxford}, issn = {0031-9422}, doi = {10.1016/j.phytochem.2017.08.018}, pages = {43 -- 51}, year = {2017}, abstract = {Mixing events in stratified lakes result in microalgae being exposed to varying conditions in light and organic carbon concentrations. Stratified lakes consist of an upper illuminated strata and a lower, darker strata where organic carbon accumulates. Therefore, in this contribution we explore the importance of dissolved organic carbon for growth under various light intensities by measuring some ecophysiological adaptations in two green microalgae. We compared the non-motile Chlorella vulgaris with the flagellated Chlamydomonas acidophila under auto-, mixo-, and heterotrophic growth conditions. In both algae the maximum photosynthetic and growth rates were highest under mixotrophy, and both algae appeared inhibited in their phosphorus acquisition under heterotrophy. Heterotrophic conditions provoked the largest differences as C. vulgaris produced chlorophyll a in darkness and grew as well as in autotrophic conditions, whereas Chl. acidophila bleached and could not grow heterotrophically. Although the fatty acid composition of both phytoplankton species differed, both species reacted in a similar way to changes in their growth conditions, mainly by a decrease of C18:3n-3 and an increase of C18:1n-9 from auto- to heterotrophic conditions. The two contrasting responses within the group of green microalgae suggest that dissolved organic carbon has a high deterministic potential to explain the survival and behaviour of green algae in the deeper strata of lakes.}, language = {en} } @article{GallUebelEbensenetal.2017, author = {Gall, Andrea and Uebel, Udo and Ebensen, Uwe and Hillebrand, Helmut and Meier, Sandra and Singer, Gabriel and Wacker, Alexander and Striebel, Maren}, title = {Planktotrons}, series = {Limnology and oceanography-methods}, volume = {15}, journal = {Limnology and oceanography-methods}, publisher = {Wiley}, address = {Hoboken}, issn = {1541-5856}, doi = {10.1002/lom3.10196}, pages = {663 -- 677}, year = {2017}, abstract = {We established a new indoor mesocosm facility, 12 fully controlled Planktotrons, designed to conduct marine and freshwater experiments for biodiversity and food web approaches using natural or artificial, benthic or planktonic communities. The Planktotrons are a unique and custom-tailored facility allowing long-term experiments. Wall growth can be inhibited by a rotating gate paddle with silicone lips. Additionally, temperature and light intensity are individually controllable for each Planktotron and the large volume (600 L) enables high-frequency or volume-intense measurements. In a pilot freshwater experiment various trophic levels of a pelagic food web were maintained for up to 90 d. First, an artificially assembled phytoplankton community of 11 species was inoculated in all Planktotrons. After 22 d, two ciliates were added to all, and three Daphnia species were added to six Planktotrons. After 72 d, dissolved organic matter (DOM, an alkaline soil extract) was added as an external disturbance to six of the 12 Planktotrons, involving three Planktotrons stocked with Daphnia and three without, respectively. We demonstrate the suitability of the Planktotrons for food web and biodiversity research. Variation among replicated Planktotrons (n=3 minimum) did not differ from other laboratory systems and field experiments. We investigated population dynamics and interactions among the different trophic levels, and found them affected by the sequence of ciliate and Daphnia addition and the disturbance caused by addition of DOM.}, language = {en} } @article{KoussoroplisSchwarzenbergerWacker2017, author = {Koussoroplis, Apostolos-Manuel and Schwarzenberger, Anke and Wacker, Alexander}, title = {Diet quality determines lipase gene expression and lipase/esterase activity in Daphnia pulex}, series = {Biology open : BiO}, volume = {6}, journal = {Biology open : BiO}, publisher = {The company of Biologists}, address = {Cambridge}, doi = {10.1242/bio.022046}, pages = {210 -- 216}, year = {2017}, abstract = {We studied the short- (12 h) and long-term (144 h) response of Daphnia pulex lipases to quality shifts in diets consisting of different mixtures of the green alga Scenedesmus with the cyanobacterium Synechococcus, two species with contrasting lipid compositions. The lipase/esterase activity in both the gut and the body tissues had fast responses to the diet shift and increased with higher dietary contributions of Synechococcus. When screening the Daphnia genome for TAG lipases, we discovered a large gene-family expansion of these enzymes. We used a subset of eight genes for mRNA expression analyses and distinguished between influences of time and diet on the observed gene expression patterns. We identified five diet-responsive lipases of which three showed a sophisticated short- and long-term pattern of expression in response to small changes in food-quality. Furthermore, the gene expression of one of the lipases was strongly correlated to lipase/esterase activity in the gut suggesting its potentially major role in digestion. These findings demonstrate that the lipid-related enzymatic machinery of D. pulex is finely tuned to diet and might constitute an important mechanism of physiological adaptation in nutritionally complex environments.}, language = {en} } @phdthesis{Bremer2017, author = {Bremer, Anne}, title = {Structural and functional characterization of three closely related intrinsically disordered proteins from the model plant Arabidopsiis thaliana}, school = {Universit{\"a}t Potsdam}, pages = {86}, year = {2017}, language = {en} } @phdthesis{DiezCocero2017, author = {Diez Cocero, Mercedes}, title = {Analysis of Rubisco - carbonic anhydrase fusions in tobacco as an approach to reduce photorespiration}, school = {Universit{\"a}t Potsdam}, pages = {116}, year = {2017}, abstract = {Rubisco catalyses the first step of CO2 assimilation into plant biomass. Despite its crucial role, it is notorious for its low catalytic rate and its tendency to fix O2 instead of CO2, giving rise to a toxic product that needs to be recycled in a process known as photorespiration. Since almost all our food supply relies on Rubisco, even small improvements in its specificity for CO2 could lead to an improvement of photosynthesis and ultimately, crop yield. In this work, we attempted to improve photosynthesis by decreasing photorespiration with an artificial CCM based on a fusion between Rubisco and a carbonic anhydrase (CA). A preliminary set of plants contained fusions between one of two CAs, bCA1 and CAH3, and the N- or C-terminus of RbcL connected by a small flexible linker of 5 amino acids. Subsequently, further fusion proteins were created between RbcL C-terminus and bCA1/CAH3 with linkers of 14, 23, 32, and 41 amino acids. The transplastomic tobacco plants carrying fusions with bCA1 were able to grow autotrophically even with the shortest linkers, albeit at a low rate, and accumulated very low levels of the fusion protein. On the other hand, plants carrying fusions with CAH3 were autotrophic only with the longer linkers. The longest linker permitted nearly wild-type like growth of the plants carrying fusions with CAH3 and increased the levels of fusion protein, but also of smaller degradation products. The fusion of catalytically inactive CAs to RbcL did not cause a different phenotype from the fusions with catalytically active CAs, suggesting that the selected CAs were not active in the fusion with RbcL or their activity did not have an effect on CO2 assimilation. However, fusions to RbcL did not abolish RbcL catalytic activity, as shown by the autotrophic growth, gas exchange and in vitro activity measurements. Furthermore, Rubisco carboxylation rate and specificity for CO2 was not altered in some of the fusion proteins, suggesting that despite the defect in RbcL folding or assembly caused by the fusions, the addition of 60-150 amino acids to RbcL does not affect its catalytic properties. On the contrary, most growth defects of the plants carrying RbcL-CA fusions are related to their reduced Rubisco content, likely caused by impaired RbcL folding or assembly. Finally, we found that fusions with RbcL C-terminus were better tolerated than with the N-terminus, and increasing the length of the linker relieved the growth impairment imposed by the fusion to RbcL. Together, the results of this work constitute considerable relevant findings for future Rubisco engineering.}, language = {en} } @article{FischbachLohBieretal.2017, author = {Fischbach, Jens and Loh, Qiuting and Bier, Frank Fabian and Lim, Theam Soon and Frohme, Marcus and Gl{\"o}kler, J{\"o}rn}, title = {Alizarin Red S for Online Pyrophosphate Detection Identified by a Rapid Screening Method}, series = {Scientific reports}, volume = {7}, journal = {Scientific reports}, publisher = {Nature Publ. Group}, address = {London}, issn = {2045-2322}, doi = {10.1038/srep45085}, pages = {9}, year = {2017}, abstract = {We identified Alizarin Red S and other well known fluorescent dyes useful for the online detection of pyrophosphate in enzymatic assays, including the loop mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) assays. An iterative screening was used for a selected set of compounds to first secure enzyme compatibility, evaluate inorganic pyrophosphate sensitivity in the presence of manganese as quencher and optimize conditions for an online detection. Of the selected dyes, the inexpensive alizarin red S was found to selectively detect pyrophosphate under LAMP and PCR conditions and is superior with respect to its defined red-shifted spectrum, long shelf life and low toxicity. In addition, the newly identified properties may also be useful in other enzymatic assays which do not generate nucleic acids but are based on inorganic pyrophosphate. Finally, we propose that our screening method may provide a blueprint for rapid screening of compounds for detecting inorganic pyrophosphate.}, language = {en} } @phdthesis{Kersting2017, author = {Kersting, Sebastian}, title = {Isothermal nucleic acid amplification for the detection of infectious pathogens}, pages = {215}, year = {2017}, language = {en} } @article{LahLoeberHsiangetal.2017, author = {Lah, Ljerka and L{\"o}ber, Ulrike and Hsiang, Tom and Hartmann, Stefanie}, title = {A genomic comparison of putative pathogenicity-related gene families in five members of the Ophiostomatales with different lifestyles}, series = {Fungal biology}, volume = {121}, journal = {Fungal biology}, publisher = {Elsevier}, address = {Oxford}, issn = {1878-6146}, doi = {10.1016/j.funbio.2016.12.002}, pages = {234 -- 252}, year = {2017}, abstract = {Ophiostomatoid fungi are vectored by their bark-beetle associates and colonize different host tree species. To survive and proliferate in the host, they have evolved mechanisms for detoxification and elimination of host defence compounds, efficient nutrient sequestration, and, in pathogenic species, virulence towards plants. Here, we assembled a draft genome of the spruce pathogen Ophiostoma bicolor. For our comparative and phylogenetic analyses, we mined the genomes of closely related species (Ophiostoma piceae, Ophiostoma ulmi, Ophiostoma novo-ulmi, and Grosmannia clavigera). Our aim was to acquire a genomic and evolutionary perspective of gene families important in host colonization. Genome comparisons showed that both the nuclear and mitochondrial genomes in our assembly were largely complete. Our O. bicolor 25.3 Mbp draft genome had 10 018 predicted genes, 6041 proteins with gene ontology (GO) annotation, 269 carbohydrate-active enzymes (CAZymes), 559 peptidases and inhibitors, and 1373 genes likely involved in pathogen-host interactions. Phylogenetic analyses of selected protein families revealed core sets of cytochrome P450 genes, ABC transporters and backbone genes involved in secondary metabolite (SM) biosynthesis (polyketide synthases (PKS) and non-ribosomal synthases), and species-specific gene losses and duplications. Phylogenetic analyses of protein families of interest provided insight into evolutionary adaptations to host biochemistry in ophiostomatoid fungi.}, language = {en} } @article{KehlmaierBarlowHastingsetal.2017, author = {Kehlmaier, Christian and Barlow, Axel and Hastings, Alexander K. and Vamberger, Melita and Paijmans, Johanna L. A. and Steadman, David W. and Albury, Nancy A. and Franz, Richard and Hofreiter, Michael and Fritz, Uwe}, title = {Tropical ancient DNA reveals relationships of the extinct bahamian giant tortoise Chelonoidis alburyorum}, series = {Proceedings of the Royal Society of London : Series B, Biological sciences}, volume = {284}, journal = {Proceedings of the Royal Society of London : Series B, Biological sciences}, publisher = {The Royal Society}, address = {London}, issn = {0962-8452}, doi = {10.1098/rspb.2016.2235}, pages = {8}, year = {2017}, abstract = {Ancient DNA of extinct species from the Pleistocene and Holocene has provided valuable evolutionary insights. However, these are largely restricted to mammals and high latitudes because DNA preservation in warm climates is typically poor. In the tropics and subtropics, non-avian reptiles constitute a significant part of the fauna and little is known about the genetics of the many extinct reptiles from tropical islands. We have reconstructed the near-complete mitochondrial genome of an extinct giant tortoise from the Bahamas (Chelonoidis alburyorum) using an approximately 1000-year-old humerus from a water-filled sinkhole (blue hole) on Great Abaco Island. Phylogenetic and molecular clock analyses place this extinct species as closely related to Galapagos (C. niger complex) and Chaco tortoises (C. chilensis), and provide evidence for repeated overseas dispersal in this tortoise group. The ancestors of extant Chelonoidis species arrived in South America from Africa only after the opening of the Atlantic Ocean and dispersed from there to the Caribbean and the Galapagos Islands. Our results also suggest that the anoxic, thermally buffered environment of blue holes may enhance DNA preservation, and thus are opening a window for better understanding evolution and population history of extinct tropical species, which would likely still exist without human impact.}, language = {en} } @article{MaddockChilderstoneFryetal.2017, author = {Maddock, Simon T. and Childerstone, Aaron and Fry, Bryan Grieg and Williams, David J. and Barlow, Axel and Wuester, Wolfgang}, title = {Multi-locus phylogeny and species delimitation of Australo-Papuan blacksnakes (Pseudechis Wagler, 1830: Elapidae: Serpentes)}, series = {Molecular phylogenetics and evolution}, volume = {107}, journal = {Molecular phylogenetics and evolution}, publisher = {Elsevier}, address = {San Diego}, issn = {1055-7903}, doi = {10.1016/j.ympev.2016.09.005}, pages = {48 -- 55}, year = {2017}, abstract = {Genetic analyses of Australasian organisms have resulted in the identification of extensive cryptic diversity across the continent. The venomous elapid snakes are among the best-studied organismal groups in this region, but many knowledge gaps persist: for instance, despite their iconic status, the species-level diversity among Australo-Papuan blacksnakes (Pseudechis) has remained poorly understood due to the existence of a group of cryptic species within the P. australis species complex, collectively termed "pygmy mulga snakes". Using two mitochondrial and three nuclear loci we assess species boundaries within the genus using Bayesian species delimitation methods and reconstruct their phylogenetic history using multispecies coalescent approaches. Our analyses support the recognition of 10 species, including all of the currently described pygmy mulga snakes and one undescribed species from the Northern Territory of Australia. Phylogenetic relationships within the genus are broadly consistent with previous work, with the recognition of three major groups, the viviparous red-bellied black snake P. porphyriacus forming the sister species to two clades consisting of ovoviviparous species.}, language = {en} } @misc{RomaoCoelhoSantosSilvaetal.2017, author = {Romao, Maria Joao and Coelho, Catarina and Santos-Silva, Teresa and Foti, Alessandro and Terao, Mineko and Garattini, Enrico and Leimk{\"u}hler, Silke}, title = {Structural basis for the role of mammalian aldehyde oxidases in the metabolism of drugs and xenobiotics}, series = {Current Opinion in Chemical Biology}, volume = {37}, journal = {Current Opinion in Chemical Biology}, publisher = {Elsevier}, address = {Oxford}, issn = {1367-5931}, doi = {10.1016/j.cbpa.2017.01.005}, pages = {39 -- 47}, year = {2017}, abstract = {Aldehyde oxidases (AOXs) are molybdo-flavoenzymes characterized by broad substrate specificity, oxidizing aromatic/aliphatic aldehydes into the corresponding carboxylic acids and hydroxylating various heteroaromatic rings. Mammals are characterized by a complement of species specific AOX isoenzymes, that varies from one in humans (AOX1) to four in rodents (AOX1, AOX2, AOX3 and AOX4). The physiological function of mammalian AOX isoenzymes is unknown, although human AOX1 is an emerging enzyme in phase-I drug metabolism. Indeed, the number of therapeutic molecules under development which act as AOX substrates is increasing. The recent crystallization and structure determination of human AOX1 as well as mouse AOX3 has brought new insights into the mechanisms underlying substrate/inhibitor binding as well as the catalytic activity of this class of enzymes.}, language = {en} } @article{SchaeferMenzJeltschetal.2017, author = {Sch{\"a}fer, Merlin and Menz, Stephan and Jeltsch, Florian and Zurell, Damaris}, title = {sOAR: a tool for modelling optimal animal life-history strategies in cyclic environments}, series = {Ecography : pattern and diversity in ecology ; research papers forum}, volume = {41}, journal = {Ecography : pattern and diversity in ecology ; research papers forum}, number = {3}, publisher = {Wiley}, address = {Hoboken}, issn = {0906-7590}, doi = {10.1111/ecog.03328}, pages = {551 -- 557}, year = {2017}, abstract = {Periodic environments determine the life cycle of many animals across the globe and the timing of important life history events, such as reproduction and migration. These adaptive behavioural strategies are complex and can only be fully understood (and predicted) within the framework of natural selection in which species adopt evolutionary stable strategies. We present sOAR, a powerful and user-friendly implementation of the well-established framework of optimal annual routine modelling. It allows determining optimal animal life history strategies under cyclic environmental conditions using stochastic dynamic programming. It further includes the simulation of population dynamics under the optimal strategy. sOAR provides an important tool for theoretical studies on the behavioural and evolutionary ecology of animals. It is especially suited for studying bird migration. In particular, we integrated options to differentiate between costs of active and passive flight into the optimal annual routine modelling framework, as well as options to consider periodic wind conditions affecting flight energetics. We provide an illustrative example of sOAR where food supply in the wintering habitat of migratory birds significantly alters the optimal timing of migration. sOAR helps improving our understanding of how complex behaviours evolve and how behavioural decisions are constrained by internal and external factors experienced by the animal. Such knowledge is crucial for anticipating potential species' response to global environmental change.}, language = {en} } @article{HaaseKrostSauteretal.2017, author = {Haase, Tobias and Krost, Annalena and Sauter, Tilman and Kratz, Karl and Peter, Jan and Kamann, Stefanie and Jung, Friedrich and Lendlein, Andreas and Zohlnh{\"o}fer, Dietlind and R{\"u}der, Constantin}, title = {In vivo biocompatibility assessment of poly (ether imide) electrospun scaffolds}, series = {Journal of Tissue Engineering and Regenerative Medicine}, volume = {11}, journal = {Journal of Tissue Engineering and Regenerative Medicine}, number = {4}, publisher = {Wiley}, address = {Hoboken}, issn = {1932-6254}, doi = {10.1002/term.2002}, pages = {1034 -- 1044}, year = {2017}, abstract = {Poly(ether imide) (PEI), which can be chemically functionalized with biologically active ligands, has emerged as a potential biomaterial for medical implants. Electrospun PEI scaffolds have shown advantageous properties, such as enhanced endothelial cell adherence, proliferation and low platelet adhesion in in vitro experiments. In this study, the in vivo behaviour of electrospun PEI scaffolds and PEI films was examined in a murine subcutaneous implantation model. Electrospun PEI scaffolds and films were surgically implanted subcutaneously in the dorsae of mice. The surrounding subcutaneous tissue response was examined via histopathological examination at 7 and 28days after implantation. No serious adverse events were observed for both types of PEI implants. The presence of macrophages or foreign body giant cells in the vicinity of the implants and the formation of a fibrous capsule indicated a normal foreign body reaction towards PEI films and scaffolds. Capsule thickness and inflammatory infiltration cells significantly decreased for PEI scaffolds during days 7-28 while remaining unchanged for PEI films. The infiltration of cells into the implant was observed for PEI scaffolds 7days after implantation and remained stable until 28days of implantation. Additionally some, but not all, PEI scaffold implants induced the formation of functional blood vessels in the vicinity of the implants. Conclusively, this study demonstrates the in vivo biocompatibility of PEI implants, with favourable properties of electrospun PEI scaffolds regarding tissue integration and wound healing.}, language = {en} } @article{KocJanuchtaHoefflerThomaetal.2017, author = {Koc-Januchta, Marta and H{\"o}ffler, Tim and Thoma, Gun-Brit and Prechtl, Helmut and Leutner, Detlev}, title = {Visualizers versus verbalizers}, series = {Computers in human behavior}, volume = {68}, journal = {Computers in human behavior}, publisher = {Elsevier}, address = {Oxford}, issn = {0747-5632}, doi = {10.1016/j.chb.2016.11.028}, pages = {170 -- 179}, year = {2017}, abstract = {This study was conducted in order to examine the differences between visualizers and verbalizers in the way they gaze at pictures and texts while learning. Using a collection of questionnaires, college students were classified according to their visual or verbal cognitive style and were asked to learn about two different, in terms of subject and type of knowledge, topics by means of text-picture combinations. Eye-tracking was used to investigate their gaze behavior. The results show that visualizers spent significantly more time inspecting pictures than verbalizers, while verbalizers spent more time inspecting texts. Results also suggest that both visualizers' and verbalizers' way of learning is active but mostly within areas providing the source of information in line with their cognitive style (pictures or text). Verbalizers tended to enter non-informative, irrelevant areas of pictures sooner than visualizers. The comparison of learning outcomes showed that the group of visualizers achieved better results than the group of verbalizers on a comprehension test.}, language = {en} } @article{MusalekKokstejnPapezetal.2017, author = {Musalek, Martin and Kokstejn, Jakub and Papez, Pavel and Scheffler, Christiane and Mumm, Rebekka and Czernitzki, Anna-Franziska and Koziel, Slawomir}, title = {Impact of normal weight obesity on fundamental motor skills in pre-school children aged 3 to 6 years}, series = {Journal of biological and clinical anthropology : Anthropologischer Anzeiger ; Mitteilungsorgan der Gesellschaft f{\"u}r Anthropologie}, volume = {74}, journal = {Journal of biological and clinical anthropology : Anthropologischer Anzeiger ; Mitteilungsorgan der Gesellschaft f{\"u}r Anthropologie}, publisher = {Schweizerbart}, address = {Stuttgart}, issn = {0003-5548}, doi = {10.1127/anthranz/2017/0752}, pages = {203 -- 212}, year = {2017}, abstract = {Normal weight obesity is defined as having excessive body fat, but normal BMI. Even though previous research revealed that excessive body fat in children inhibited their physical activity and decreased motor performance, there has been only little evidence about motor performance of normal weight obese children. This study aims to establish whether normal weight obese pre-school children aged 3-6 years will have a significantly worse level of fundamental motor skills compared to normal weight non-obese counterparts. The research sample consisted of 152 pre-schoolers selected from a specific district of Prague, the Czech Republic. According to values from four skinfolds: triceps, subscapula, suprailiaca, calf, and BMI three categories of children aged 3-6 years were determined: A) normal weight obese n = 51; B) normal weight non-obese n = 52; C) overweight and obese n = 49. The Movement Assessment Battery for Children (MABC-2) was used for the assessment of fundamental motor skills. Normal weight obese children had significantly higher amount of adipose tissue p < 0.001 than normal weight non-obese children but the same average BMI. Moreover, normal weight obese children did not have significantly less amount of subcutaneous fat on triceps and calf compared to their overweight and obese peers. In majority of MABC-2 tests, normal weight obese pre-schoolers showed the poorest performance. Moreover, normal weight obese children had significantly worse total standard score = 38.82 compared to normal weight non-obese peers = 52.27; p < 0.05. In addition, normal weight obese children had a more than three times higher frequency OR = 3.69 CI95\% (1.10; 12.35) of severe motor deficit performance <= 5th centile of the MABC-2 norm. These findings are strongly alarming since indices like BMI are not able to identify normal weight obese individual. We recommend verifying real portion of normal weight obese children as they are probably in higher risk of health and motor problems than overweight and obese population due to their low lean mass.}, language = {en} } @article{GomulaKozielGrothetal.2017, author = {Gomula, Aleksandra and Koziel, Slawomir and Groth, Detlef and Bielicki, Tadeusz}, title = {The effect of neighboring districts on body height of Polish conscripts}, series = {Anthropologischer Anzeiger : journal of biological and clinical anthropology ; Mitteilungsorgan der Gesellschaft f{\"u}r Anthropologie}, volume = {74}, journal = {Anthropologischer Anzeiger : journal of biological and clinical anthropology ; Mitteilungsorgan der Gesellschaft f{\"u}r Anthropologie}, number = {1}, publisher = {Schweizerbart}, address = {Stuttgart}, issn = {0003-5548}, doi = {10.1127/anthranz/2017/0701}, pages = {71 -- 76}, year = {2017}, abstract = {The aim of the study was to investigate the correlation of heights of conscripts living in neighboring districts in Poland. The study used 10\% of a nationally representative sample of 26,178 males 18.5-19.5 years old examined during the National survey of Polish conscripts conducted in 2001. The sample represented all regions and social strata of the country and included 354 different districts within 16 voivodships (provinces). Analyses were performed with the R statistical software. A small but significant correlation (0.24, p < 0.0001) was observed for height between 1st order neighboring districts. Correlations decreased with increased distances between neighboring districts, but remained significant for 7th node neighbors (0.18, p < 0.0001). Regarding voivodships (provinces), average height showed a geographical trend from the northwest (relatively tall) to the southeast (relatively short), and the correlation was stronger for first order neighboring provinces (0.796, p < 0.001). This study revealed clusters of tall people and short people, providing a support for hypothesis of the community effect in height. Small correlations between 1st order neighbors than in another country (Switzerland) may be associated with differences in geography, since in Poland there are no natural barriers (e.g., mountains) and road infrastructure is well-developed.}, language = {en} } @article{HolzloehnerHanack2017, author = {Holzl{\"o}hner, Pamela and Hanack, Katja}, title = {Generation of murine monoclonal antibodies by hybridoma technology}, series = {JoVE : Video journal}, journal = {JoVE : Video journal}, number = {119}, publisher = {JoVE}, address = {Cambridge}, issn = {1940-087X}, doi = {10.3791/54832}, pages = {7}, year = {2017}, abstract = {Monoclonal antibodies are universal binding molecules and are widely used in biomedicine and research. Nevertheless, the generation of these binding molecules is time-consuming and laborious due to the complicated handling and lack of alternatives. The aim of this protocol is to provide one standard method for the generation of monoclonal antibodies using hybridoma technology. This technology combines two steps. Step 1 is an appropriate immunization of the animal and step 2 is the fusion of B lymphocytes with immortal myeloma cells in order to generate hybrids possessing both parental functions, such as the production of antibody molecules and immortality. The generated hybridoma cells were then recloned and diluted to obtain stable monoclonal cell cultures secreting the desired monoclonal antibody in the culture supernatant. The supernatants were tested in enzyme-linked immunosorbent assays (ELISA) for antigen specificity. After the selection of appropriate cell clones, the cells were transferred to mass cultivation in order to produce the desired antibody molecule in large amounts. The purification of the antibodies is routinely performed by affinity chromatography. After purification, the antibody molecule can be characterized and validated for the final test application. The whole process takes 8 to 12 months of development, and there is a high risk that the antibody will not work in the desired test system.}, language = {en} } @article{LuetkecosmannWarsinkeTschoepeetal.2017, author = {L{\"u}tkecosmann, Steffi and Warsinke, Axel and Tsch{\"o}pe, Winfried and Eichler, R{\"u}diger and Hanack, Katja}, title = {A novel monoclonal antibody suitable for the detection of leukotriene B4}, series = {Biochemical and biophysical research communications}, volume = {482}, journal = {Biochemical and biophysical research communications}, number = {4}, publisher = {Elsevier}, address = {San Diego}, issn = {0006-291X}, doi = {10.1016/j.bbrc.2016.11.157}, pages = {1054 -- 1059}, year = {2017}, abstract = {Leukotriene B4 as an inflammatory mediator is an important biomarker for different respiratory diseases like asthma, chronic obstructive pulmonary disease or cystic lung fibrosis. Therefore the detection of LTB4 is helpful in the diagnosis of these pulmonary diseases. However, until now its determination in exhaled breath condensates suffers from problems of accuracy. Reasons for that could be improper sample collection and preparation methods of condensates and the lack of consistently assay specificity and reproducibility of the used immunoassay detection system. In this study we describe the development and the characterization of a specific monoclonal antibody (S27BC6) against LTB4, its use as molecular recognition element for the development of an enzyme-linked immunoassay to detect LTB4 and discuss possible future diagnostic applications.}, language = {en} } @phdthesis{deAbreueLima2017, author = {de Abreu e Lima, Francisco Anastacio}, title = {Experimental validation of hybrid performance predictive models in Zea mays L.}, school = {Universit{\"a}t Potsdam}, pages = {127}, year = {2017}, language = {en} } @phdthesis{Bajdzienko2017, author = {Bajdzienko, Krzysztof}, title = {Analysis of Target of Rapamycin (Tor) induced changes of the Arabidopsis thaliana proteome using sub-cellular resolution}, school = {Universit{\"a}t Potsdam}, pages = {167}, year = {2017}, language = {en} } @phdthesis{Kubsch2017, author = {Kubsch, Bastian}, title = {Phase-specific fusion between biomembranes using SNARE mimetics}, school = {Universit{\"a}t Potsdam}, pages = {95}, year = {2017}, language = {en} } @phdthesis{Gorka2017, author = {G{\´o}rka, Michal Jakub}, title = {Establishing a pipeline for identification of protein- protein interactions using different native fractionation methods}, school = {Universit{\"a}t Potsdam}, pages = {109}, year = {2017}, language = {en} } @phdthesis{MartinezJaime2017, author = {Mart{\´i}nez Jaime, Silvia}, title = {Towards the understanding of protein function and regulation}, school = {Universit{\"a}t Potsdam}, pages = {131}, year = {2017}, language = {en} } @phdthesis{Yang2017, author = {Yang, Lei}, title = {Verification of systemic mRNAs mobility and mobile functions}, school = {Universit{\"a}t Potsdam}, pages = {125}, year = {2017}, language = {en} } @phdthesis{Barbirz2017, author = {Barbirz, Stefanie}, title = {Highly specific binders for bacterial polysaccharides}, school = {Universit{\"a}t Potsdam}, pages = {167}, year = {2017}, language = {en} } @phdthesis{DiezCocero2017, author = {Diez Cocero, Mercedes}, title = {Analysis of Rubisco - carbonic anhydrase fusions in tobacco as an approach to reduce photorespiration}, school = {Universit{\"a}t Potsdam}, pages = {116}, year = {2017}, language = {en} } @phdthesis{Belkius2017, author = {Belkius, Karolina Dorota}, title = {Systems biology approach to investigate the development and degradation of the photosynthetic apparatus during leaf ontogenesis in higher plants}, school = {Universit{\"a}t Potsdam}, pages = {121}, year = {2017}, language = {en} } @phdthesis{Zeng2017, author = {Zeng, Ting}, title = {Nanoparticles promoted biocatalysis}, school = {Universit{\"a}t Potsdam}, pages = {99}, year = {2017}, language = {en} } @phdthesis{PandeyPant2017, author = {Pandey-Pant, Pooja}, title = {Comparative transcriptomics and functional genomics during phosphorus limitation in plants}, school = {Universit{\"a}t Potsdam}, pages = {152}, year = {2017}, language = {en} } @article{FerTietjenJeltschetal.2017, author = {Fer, Istem and Tietjen, Britta and Jeltsch, Florian and Wolff, Christian Michael}, title = {The influence of El Nino-Southern Oscillation regimes on eastern African vegetation and its future implications under the RCP8.5 warming scenario}, series = {Biogeosciences}, volume = {14}, journal = {Biogeosciences}, number = {18}, publisher = {Copernicus}, address = {Katlenburg-Lindau}, issn = {1726-4170}, doi = {10.5194/bg-14-4355-2017}, pages = {4355 -- 4374}, year = {2017}, abstract = {The El Nino-Southern Oscillation (ENSO) is the main driver of the interannual variability in eastern African rainfall, with a significant impact on vegetation and agriculture and dire consequences for food and social security. In this study, we identify and quantify the ENSO contribution to the eastern African rainfall variability to forecast future eastern African vegetation response to rainfall variability related to a predicted intensified ENSO. To differentiate the vegetation variability due to ENSO, we removed the ENSO signal from the climate data using empirical orthogonal teleconnection (EOT) analysis. Then, we simulated the ecosystem carbon and water fluxes under the historical climate without components related to ENSO teleconnections. We found ENSO-driven patterns in vegetation response and confirmed that EOT analysis can successfully produce coupled tropical Pacific sea surface temperature-eastern African rainfall teleconnection from observed datasets. We further simulated eastern African vegetation response under future climate change as it is projected by climate models and under future climate change combined with a predicted increased ENSO intensity. Our EOT analysis highlights that climate simulations are still not good at capturing rainfall variability due to ENSO, and as we show here the future vegetation would be different from what is simulated under these climate model outputs lacking accurate ENSO contribution. We simulated considerable differences in eastern African vegetation growth under the influence of an intensified ENSO regime which will bring further environmental stress to a region with a reduced capacity to adapt effects of global climate change and food security.}, language = {en} } @phdthesis{Wu2017, author = {Wu, Si}, title = {Exploring the Arabidopsis metabolic landscape by genetic mapping integrated with network analysis}, school = {Universit{\"a}t Potsdam}, pages = {121}, year = {2017}, language = {en} } @phdthesis{Loiacono2017, author = {Loiacono, Filomena Vanessa}, title = {Transfer of chloroplast RNA editing events between species}, school = {Universit{\"a}t Potsdam}, pages = {155}, year = {2017}, language = {en} } @article{KloseRolkeBaumann2017, author = {Klose, Sascha Peter and Rolke, Daniel and Baumann, Otto}, title = {Morphogenesis of honeybee hypopharyngeal gland during pupal development}, series = {Frontiers in zoology}, volume = {14}, journal = {Frontiers in zoology}, publisher = {BioMed Central}, address = {London}, issn = {1742-9994}, doi = {10.1186/s12983-017-0207-z}, year = {2017}, abstract = {Background The hypopharyngeal gland of worker bees contributes to the production of the royal jelly fed to queens and larvae. The gland consists of thousands of two-cell units that are composed of a secretory cell and a duct cell and that are arranged in sets of about 12 around a long collecting duct. Results By fluorescent staining, we have examined the morphogenesis of the hypopharyngeal gland during pupal life, from a saccule lined by a pseudostratified epithelium to the elaborate organ of adult worker bees. The hypopharyngeal gland develops as follows. (1) Cell proliferation occurs during the first day of pupal life in the hypopharyngeal gland primordium. (2) Subsequently, the epithelium becomes organized into rosette-like units of three cells. Two of these will become the secretory cell and the duct cell of the adult secretory units; the third cell contributes only temporarily to the development of the secretory units and is eliminated by apoptosis in the second half of pupal life. (3) The three-cell units of flask-shaped cells undergo complex changes in cell morphology. Thus, by mid-pupal stage, the gland is structurally similar to the adult hypopharyngeal gland. (4) Concomitantly, the prospective secretory cell attains its characteristic subcellular organization by the invagination of a small patch of apical membrane domain, its extension to a tube of about 100 μm in length (termed a canaliculus), and the expansion of the tube to a diameter of about 3 μm. (6) Finally, the canaliculus-associated F-actin system becomes reorganized into rings of bundled actin filaments that are positioned at regular distances along the membrane tube. Conclusions The morphogenesis of the secretory units in the hypopharyngeal gland of the worker bee seems to be based on a developmental program that is conserved, with slight modification, among insects for the production of dermal glands. Elaboration of the secretory cell as a unicellular seamless epithelial tube occurs by invagination of the apical membrane, its extension likely by targeted exocytosis and its expansion, and finally the reorganisation of the membrane-associated F-actin system. Our work is fundamental for future studies of environmental effects on hypopharyngeal gland morphology and development.}, language = {en} } @article{ShubchynskyyBonieckaSchweighoferetal.2017, author = {Shubchynskyy, Volodymyr and Boniecka, Justyna and Schweighofer, Alois and Simulis, Justinas and Kvederaviciute, Kotryna and Stumpe, Michael and Mauch, Felix and Balazadeh, Salma and M{\"u}ller-R{\"o}ber, Bernd and Boutrot, Freddy and Zipfel, Cyril and Meskiene, Irute}, title = {Protein phosphatase AP2C1 negatively regulates basal resistance and defense responses to Pseudomonas syringae}, series = {Journal of experimental botany}, volume = {68}, journal = {Journal of experimental botany}, number = {5}, publisher = {Oxford Univ. Press}, address = {Oxford}, issn = {0022-0957}, doi = {10.1093/jxb/erw485}, pages = {1169 -- 1183}, year = {2017}, abstract = {Mitogen-activated protein kinases (MAPKs) mediate plant immune responses to pathogenic bacteria. However, less is known about the cell autonomous negative regulatory mechanism controlling basal plant immunity. We report the biological role of Arabidopsis thaliana MAPK phosphatase AP2C1 as a negative regulator of plant basal resistance and defense responses to Pseudomonas syringae. AP2C2, a closely related MAPK phosphatase, also negatively controls plant resistance. Loss of AP2C1 leads to enhanced pathogen-induced MAPK activities, increased callose deposition in response to pathogen-associated molecular patterns or to P. syringae pv. tomato (Pto) DC3000, and enhanced resistance to bacterial infection with Pto. We also reveal the impact of AP2C1 on the global transcriptional reprogramming of transcription factors during Pto infection. Importantly, ap2c1 plants show salicylic acid-independent transcriptional reprogramming of several defense genes and enhanced ethylene production in response to Pto. This study pinpoints the specificity of MAPK regulation by the different MAPK phosphatases AP2C1 and MKP1, which control the same MAPK substrates, nevertheless leading to different downstream events. We suggest that precise and specific control of defined MAPKs by MAPK phosphatases during plant challenge with pathogenic bacteria can strongly influence plant resistance.}, language = {en} } @article{MachensBalazadehMuellerRoeberetal.2017, author = {Machens, Fabian and Balazadeh, Salma and M{\"u}ller-R{\"o}ber, Bernd and Messerschmidt, Katrin}, title = {Synthetic Promoters and Transcription Factors for Heterologous Protein Expression in Saccharomyces cerevisiae}, series = {Frontiers in Bioengineering and Biotechnology}, volume = {5}, journal = {Frontiers in Bioengineering and Biotechnology}, publisher = {Frontiers}, address = {Lausanne}, issn = {2296-4185}, doi = {10.3389/fbioe.2017.00063}, pages = {1 -- 11}, year = {2017}, abstract = {Orthogonal systems for heterologous protein expression as well as for the engineering of synthetic gene regulatory circuits in hosts like Saccharomyces cerevisiae depend on synthetic transcription factors (synTFs) and corresponding cis-regulatory binding sites. We have constructed and characterized a set of synTFs based on either transcription activator-like effectors or CRISPR/Cas9, and corresponding small synthetic promoters (synPs) with minimal sequence identity to the host's endogenous promoters. The resulting collection of functional synTF/synP pairs confers very low background expression under uninduced conditions, while expression output upon induction of the various synTFs covers a wide range and reaches induction factors of up to 400. The broad spectrum of expression strengths that is achieved will be useful for various experimental setups, e.g., the transcriptional balancing of expression levels within heterologous pathways or the construction of artificial regulatory networks. Furthermore, our analyses reveal simple rules that enable the tuning of synTF expression output, thereby allowing easy modification of a given synTF/synP pair. This will make it easier for researchers to construct tailored transcriptional control systems.}, language = {en} } @phdthesis{Sedaghatmehr2017, author = {Sedaghatmehr, Mastoureh}, title = {Unraveling the regulatory mechanisms of heat stress memory in Arabidopsis thaliana}, school = {Universit{\"a}t Potsdam}, pages = {176}, year = {2017}, language = {en} } @article{ReinickeReesEspeeletal.2017, author = {Reinicke, Stefan and Rees, Huw C. and Espeel, Pieter and Vanparijs, Nane and Bisterfeld, Carolin and Dick, Markus and Rosencrantz, Ruben R. and Brezesinski, Gerald and de Geest, Bruno G. and Du Prez, Filip E. and Pietruszka, J{\"o}rg and B{\"o}ker, Alexander}, title = {Immobilization of 2-Deoxy-D-ribose-5-phosphate Aldolase in Polymeric Thin Films via the Langmuir-Schaefer Technique}, series = {ACS applied materials \& interfaces}, volume = {9}, journal = {ACS applied materials \& interfaces}, publisher = {American Chemical Society}, address = {Washington}, issn = {1944-8244}, doi = {10.1021/acsami.6b13632}, pages = {8317 -- 8326}, year = {2017}, abstract = {A synthetic protocol for the fabrication of ultrathin polymeric films containing the enzyme 2-deoxy-D-ribose-5-phosphate aldolase from Escherichia coli (DERA(EC)) is presented. Ultrathin enzymatically active films are useful for applications in which only small quantities of active material are needed and at the same time quick response and contact times without diffusion limitation are wanted. We show how DERA as an exemplary enzyme can be immobilized in a thin polymer layer at the air-water interface and transferred to a suitable support by the Langmuir-Schaefer technique under full conservation of enzymatic activity. The polymer in use is a poly(N-isopropylacrylamide-co-N-2-thiolactone acrylamide) (P(NIPAAm-co-TlaAm)) statistical copolymer in which the thiolactone units serve a multitude of purposes including hydrophobization of the polymer, covalent binding of the enzyme and the support and finally cross-linking of the polymer matrix. The application of this type of polymer keeps the whole approach simple as additional cocomponents such as cross-linkers are avoided.}, language = {en} } @misc{KozielHermanussenGomulaetal.2017, author = {Koziel, Slawomir and Hermanussen, Michael and Gomula, Alexandra and Swanson, James and Kaczmarek, Maria and El-Shabrawi, Mortada and Elhusseini, Mona and Satake, Takashi and Martinovic Klaric, Irena and Scheffler, Christiane and Morkuniene, Ruta and Godina, Elena and Sasa, Missoni and Tutkuviene, Janina and Siniarska, Anna and Nieczuja-Dwojacka, Joanna and Nunez, Javier and Groth, Detlef and Barbieri, Davide}, title = {Adolescence - a Transition to Adulthood Proceedings of the 24th Aschauer Soiree, held at Jurata, Poland, November 5th 2016}, series = {Pediatric Endocrinology Reviews}, volume = {14}, journal = {Pediatric Endocrinology Reviews}, number = {3}, publisher = {Medical Media}, address = {Netanya}, issn = {1565-4753}, pages = {326 -- 334}, year = {2017}, abstract = {Eighteen scientists met at Jurata, Poland, to discuss various aspects of the transition from adolescence to adulthood. This transition is a delicate period facing complex interactions between the adolescents and the social group they belong to. Social identity, group identification and identity signalling, but also stress affecting basal salivary cortisol rhythms, hypertension, inappropriate nutrition causing latent and manifest obesity, moreover, in developing and under-developed countries, parasitosis causing anaemia thereby impairing growth and development, are issues to be dealt with during this period of the human development. In addition, some new aspects of the association between weight, height and head circumference in the newborns were discussed, as well as intrauterine head growth and head circumference as health risk indicators.}, language = {en} } @article{BoginSchefflerHermanussen2017, author = {Bogin, Barry and Scheffler, Christiane and Hermanussen, Michael}, title = {Global effects of income and income inequality on adult height and sexual dimorphism in height}, series = {American journal of human biology : the official journal of the Human Biology Council}, volume = {29}, journal = {American journal of human biology : the official journal of the Human Biology Council}, number = {2}, publisher = {Wiley}, address = {Hoboken}, issn = {1042-0533}, doi = {10.1002/ajhb.22980}, pages = {11}, year = {2017}, abstract = {Objectives: Average adult height of a population is considered a biomarker of the quality of the health environment and economic conditions. The causal relationships between height and income inequality are not well understood. We analyze data from 169 countries for national average heights of men and women and national-level economic factors to test two hypotheses: (1) income inequality has a greater association with average adult height than does absolute income; and (2) neither income nor income inequality has an effect on sexual dimorphism in height. Methods: Average height data come from the NCD-RisC health risk factor collaboration. Economic indicators are derived from the World Bank data archive and include gross domestic product (GDP), Gross National Income per capita adjusted for personal purchasing power (GNI_ PPP), and income equality assessed by the Gini coefficient calculated by the Wagstaff method. Results: Hypothesis 1 is supported. Greater income equality is most predictive of average height for both sexes. GNI_ PPP explains a significant, but smaller, amount of the variation. National GDP has no association with height. Hypothesis 2 is rejected. With greater average adult height there is greater sexual dimorphism. Conclusions: Findings support a growing literature on the pernicious effects of inequality on growth in height and, by extension, on health. Gradients in height reflect gradients in social disadvantage. Inequality should be considered a pollutant that disempowers people from the resources needed for their own healthy growth and development and for the health and good growth of their children.}, language = {en} } @article{SchefflerGreilHermanussen2017, author = {Scheffler, Christiane and Greil, Holle and Hermanussen, Michael}, title = {The association between weight, height, and head circumference reconsidered}, series = {Pediatric Research}, volume = {81}, journal = {Pediatric Research}, publisher = {Nature Publ. Group}, address = {New York}, issn = {0031-3998}, doi = {10.1038/pr.2017.3}, pages = {825 -- 830}, year = {2017}, abstract = {BACKGROUND: Under normal nutritional and health conditions, body height, weight and head circumference are significantly related. We hypothesize that the apparent general association between weight, height, and head circumference of the growing child might be misleading. METHODS: We reanalyzed data of 7,444 boys and 7,375 girls measured in East-Germany between 1986 and 1990, aged from 0 to 7 y with measurements of body length/height, leg length, sitting height, biacromial shoulder breadth, thoracic breadth, thoracic depth, thoracic circumference, body weight, head volume, percentage of body fat, and hip skinfold vertical, using principal component analysis. RESULTS: Strong associations exist between skeletal growth, fat accumulation, and head volume increments. Yet in spite of this general proportionality, skeletal growth, fat acquisition, and head growth exhibit different patterns. Three components explain between almost 60\% and more than 75\% of cumulative variance between birth and age 7 y. Parameters of skeletal growth predominantly load on the first component and clearly separate from indicators of fat deposition. After age of 2 y, head volume loads on a separate third component in both sexes indicating independence of head growth. CONCLUSION: Under appropriate nutritional and health circumstances, nutritional status, body size, and head circumference are not related.}, language = {en} } @phdthesis{Radon2017, author = {Radon, Christin}, title = {Analyse der Funktion der dualen Lokalisation der 3-Mercaptopyruvat Sulfurtransferase im Menschen}, school = {Universit{\"a}t Potsdam}, pages = {123}, year = {2017}, language = {de} } @phdthesis{Weiss2017, author = {Weiß, Lina}, title = {Understanding the emergence and maintenance of biodiversity in grasslands}, school = {Universit{\"a}t Potsdam}, pages = {153}, year = {2017}, language = {en} }