@article{HoehnJerniganJaptoketal.2017, author = {Hoehn, Richard S. and Jernigan, Peter L. and Japtok, Lukasz and Chang, Alex L. and Midura, Emily F. and Caldwell, Charles C. and Kleuser, Burkhard and Lentsch, Alex B. and Edwards, Michael J. and Gulbins, Erich and Pritts, Timothy A.}, title = {Acid sphingomyelinase inhibition in stored erythrocytes reduces transfusion-associated lung inflammation}, series = {Annals of surgery : a monthly review of surgical science and practice}, volume = {265}, journal = {Annals of surgery : a monthly review of surgical science and practice}, number = {1}, publisher = {Lippincott Williams \& Wilkins}, address = {Philadelphia}, issn = {0003-4932}, doi = {10.1097/SLA.0000000000001648}, pages = {218 -- 226}, year = {2017}, abstract = {Objective: We aimed to identify the role of the enzyme acid sphingomyelinase in the aging of stored units of packed red blood cells (pRBCs) and subsequent lung inflammation after transfusion. Summary Background Data: Large volume pRBC transfusions are associated with multiple adverse clinical sequelae, including lung inflammation. Microparticles are formed in stored pRBCs over time and have been shown to contribute to lung inflammation after transfusion. Methods: Human and murine pRBCs were stored with or without amitriptyline, a functional inhibitor of acid sphingomyelinase, or obtained from acid sphingomyelinase-deficient mice, and lung inflammation was studied in mice receiving transfusions of pRBCs and microparticles isolated from these units. Results: Acid sphingomyelinase activity in pRBCs was associated with the formation of ceramide and the release of microparticles. Treatment of pRBCs with amitriptyline inhibited acid sphingomyelinase activity, ceramide accumulation, and microparticle production during pRBC storage. Transfusion of aged pRBCs or microparticles isolated from aged blood into mice caused lung inflammation. This was attenuated after transfusion of pRBCs treated with amitriptyline or from acid sphingomyelinase-deficient mice. Conclusions: Acid sphingomyelinase inhibition in stored pRBCs offers a novel mechanism for improving the quality of stored blood.}, language = {en} } @article{HolzloehnerHanack2017, author = {Holzl{\"o}hner, Pamela and Hanack, Katja}, title = {Generation of murine monoclonal antibodies by hybridoma technology}, series = {JoVE : Video journal}, journal = {JoVE : Video journal}, number = {119}, publisher = {JoVE}, address = {Cambridge}, issn = {1940-087X}, doi = {10.3791/54832}, pages = {7}, year = {2017}, abstract = {Monoclonal antibodies are universal binding molecules and are widely used in biomedicine and research. Nevertheless, the generation of these binding molecules is time-consuming and laborious due to the complicated handling and lack of alternatives. The aim of this protocol is to provide one standard method for the generation of monoclonal antibodies using hybridoma technology. This technology combines two steps. Step 1 is an appropriate immunization of the animal and step 2 is the fusion of B lymphocytes with immortal myeloma cells in order to generate hybrids possessing both parental functions, such as the production of antibody molecules and immortality. The generated hybridoma cells were then recloned and diluted to obtain stable monoclonal cell cultures secreting the desired monoclonal antibody in the culture supernatant. The supernatants were tested in enzyme-linked immunosorbent assays (ELISA) for antigen specificity. After the selection of appropriate cell clones, the cells were transferred to mass cultivation in order to produce the desired antibody molecule in large amounts. The purification of the antibodies is routinely performed by affinity chromatography. After purification, the antibody molecule can be characterized and validated for the final test application. The whole process takes 8 to 12 months of development, and there is a high risk that the antibody will not work in the desired test system.}, language = {en} } @article{HornickBachCrawfurdetal.2017, author = {Hornick, Thomas and Bach, Lennart T. and Crawfurd, Katharine J. and Spilling, Kristian and Achterberg, Eric P. and Woodhouse, Jason Nicholas and Schulz, Kai G. and Brussaard, Corina P. D. and Riebesell, Ulf and Grossart, Hans-Peter}, title = {Ocean acidification impacts bacteria-phytoplankton coupling at low-nutrient conditions}, series = {Biogeosciences}, volume = {14}, journal = {Biogeosciences}, number = {1}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {1726-4170}, doi = {10.5194/bg-14-1-2017}, pages = {1 -- 15}, year = {2017}, abstract = {The oceans absorb about a quarter of the annually produced anthropogenic atmospheric carbon dioxide (CO2), resulting in a decrease in surface water pH, a process termed ocean acidification (OA). Surprisingly little is known about how OA affects the physiology of heterotrophic bacteria or the coupling of heterotrophic bacteria to phytoplankton when nutrients are limited. Previous experiments were, for the most part, undertaken during productive phases or following nutrient additions designed to stimulate algal blooms. Therefore, we performed an in situ large-volume mesocosm (similar to 55 m(3)) experiment in the Baltic Sea by simulating different fugacities of CO2 (fCO(2)) extending from present to future conditions. The study was conducted in July-August after the nominal spring bloom, in order to maintain low-nutrient conditions throughout the experiment. This resulted in phytoplankton communities dominated by small-sized functional groups (picophytoplankton). There was no consistent fCO(2)-induced effect on bacterial protein production (BPP), cell-specific BPP (csBPP) or biovolumes (BVs) of either free-living (FL) or particle-associated (PA) heterotrophic bacteria, when considered as individual components (univariate analyses). Permutational Multivariate Analysis of Variance (PERMANOVA) revealed a significant effect of the fCO(2) treatment on entire assemblages of dissolved and particulate nutrients, metabolic parameters and the bacteria-phytoplankton community. However, distance-based linear modelling only identified fCO(2) as a factor explaining the variability observed amongst the microbial community composition, but not for explaining variability within the metabolic parameters. This suggests that fCO(2) impacts on microbial metabolic parameters occurred indirectly through varying physicochemical parameters and microbial species composition. Cluster analyses examining the co-occurrence of different functional groups of bacteria and phytoplankton further revealed a separation of the four fCO(2)-treated mesocosms from both control mesocosms, indicating that complex trophic interactions might be altered in a future acidified ocean. Possible consequences for nutrient cycling and carbon export are still largely unknown, in particular in a nutrient-limited ocean.}, language = {en} } @article{IetswaartRosaWuetal.2017, author = {Ietswaart, Robert and Rosa, Stefanie and Wu, Zhe and Dean, Caroline and Howard, Martin}, title = {Cell-Size-Dependent Transcription of FLC and Its Antisense Long Non-coding RNA COOLAIR Explain Cell-to-Cell Expression Variation}, series = {Cell systems}, volume = {4}, journal = {Cell systems}, publisher = {Cell Press}, address = {Cambridge}, issn = {2405-4712}, doi = {10.1016/j.cels.2017.05.010}, pages = {622 -- 635}, year = {2017}, abstract = {Single-cell quantification of transcription kinetics and variability promotes a mechanistic understanding of gene regulation. Here, using single-molecule RNA fluorescence in situ hybridization and mathematical modeling, we dissect cellular RNA dynamics for Arabidopsis FLOWERING LOCUS C (FLC). FLC expression quantitatively determines flowering time and is regulated by antisense (COOLAIR) transcription. In cells without observable COOLAIR expression, we quantify FLC transcription initiation, elongation, intron processing, and lariat degradation, as well as mRNA release from the locus and degradation. In these heterogeneously sized cells, FLC mRNA number increases linearly with cell size, resulting in a large cell-to-cell variability in transcript level. This variation is accounted for by cell-sizedependent, Poissonian FLC mRNA production, but not by large transcriptional bursts. In COOLAIRexpressing cells, however, antisense transcription increases with cell size and contributes to FLC transcription decreasing with cell size. Our analysis therefore reveals an unexpected role for antisense transcription in modulating the scaling of transcription with cell size.}, language = {en} } @phdthesis{Janowski2017, author = {Janowski, Marcin Andrzej}, title = {Investigating role of the essential GTPase - AtRsgA in the assembly of the small ribosomal subunit in Arabidopsis thaliana chloroplast}, school = {Universit{\"a}t Potsdam}, pages = {114}, year = {2017}, language = {en} } @phdthesis{Janowski2017, author = {Janowski, Marcin Andrzej}, title = {Investigating role of the essential GTPase - AtRsgA in the assembly of the small ribosomal subunit in Arabidopsis thaliana chloroplast}, school = {Universit{\"a}t Potsdam}, pages = {X, 114}, year = {2017}, abstract = {Plastid protein biosynthesis occurs on bacterial-type 70S ribosomes consisting of a large (50S) and a small (30S) subunit. However, since many steps of ribosome biogenesis are not thermodynamically favorable at biological conditions, it requires many assembly factors. One group of assembly factors, circularly permuted GTPases, was implicated in 30S subunit maturation in E. coli, by a protein RsgA. RsgA orthologues are present in bacteria and plastid-containing species and in silico analysis revealed presence of a RsgA-like protein in Arabidopsis thaliana. To functionally characterize the Arabidopsis orthologue, two AtRsgA T-DNA insertion lines were analyzed in this study. The exon line (rsgA-e) led to embryo lethality, while the intron line (rsgA-i) caused severe dwarf, pale green phenotype. Further investigation of rsgA-i mutant line revealed defects in chloroplast biogenesis which led to increased number of chloroplasts, decreased chloroplast size, decreased air space between mesophyll cells and smaller shoot apical meristems, which showed unusual proplastid accumulation. Moreover, rsgA-i plants showed reduction in chlorophyll A and B content, decreased electron transport rate and photosynthetic efficiency. Further analyses revealed that the protein is involved in chloroplast 30S subunit maturation. Interestingly, we observed that while chloroplast-targeted and chloroplast-encoded proteins are generally downregulated in the mutant, a contrasting upregulation of the corresponding transcripts is observed, indicating an elaborate compensatory mechanism. To conclude, the study presented here reveals a ribosome assembly factor and a compensatory mechanism activated during impaired chloroplast function.}, language = {en} } @article{KangLimOhetal.2017, author = {Kang, Mi-Sun and Lim, Hae-Soon and Oh, Jong-Suk and Lim, You-jin and Wuertz-Kozak, Karin and Harro, Janette M. and Shirtliff, Mark E. and Achermann, Yvonne}, title = {Antimicrobial activity of Lactobacillus salivarius and Lactobacillus fermentum against Staphylococcus aureus}, series = {Pathogens and disease / Federation of European Microbiology Societies}, volume = {75}, journal = {Pathogens and disease / Federation of European Microbiology Societies}, number = {2}, publisher = {Oxford Univ. Press}, address = {Oxford}, issn = {2049-632X}, doi = {10.1093/femspd/ftx009}, pages = {10}, year = {2017}, abstract = {The increasing prevalence of methicillin-resistant Staphylococcus aureus has become a major public health threat. While lactobacilli were recently found useful in combating various pathogens, limited data exist on their therapeutic potential for S. aureus infections. The aim of this study was to determine whether Lactobacillus salivarius was able to produce bactericidal activities against S. aureus and to determine whether the inhibition was due to a generalized reduction in pH or due to secreted Lactobacillus product(s). We found an 8.6-log10 reduction of planktonic and a 6.3-log10 reduction of biofilm S. aureus. In contrast, the previously described anti-staphylococcal effects of L. fermentum only caused a 4.0-log10 reduction in planktonic S. aureus cells, with no effect on biofilm S. aureus cells. Killing of S. aureus was partially pH dependent, but independent of nutrient depletion. Cell-free supernatant that was pH neutralized and heat inactivated or proteinase K treated had significantly reduced killing of L. salivarius than with pH-neutralized supernatant alone. Proteomic analysis of the L. salivarius secretome identified a total of five secreted proteins including a LysM-containing peptidoglycan binding protein and a protein peptidase M23B. These proteins may represent potential novel anti-staphylococcal agents that could be effective against S. aureus biofilms.}, language = {en} } @article{KehlmaierBarlowHastingsetal.2017, author = {Kehlmaier, Christian and Barlow, Axel and Hastings, Alexander K. and Vamberger, Melita and Paijmans, Johanna L. A. and Steadman, David W. and Albury, Nancy A. and Franz, Richard and Hofreiter, Michael and Fritz, Uwe}, title = {Tropical ancient DNA reveals relationships of the extinct bahamian giant tortoise Chelonoidis alburyorum}, series = {Proceedings of the Royal Society of London : Series B, Biological sciences}, volume = {284}, journal = {Proceedings of the Royal Society of London : Series B, Biological sciences}, publisher = {The Royal Society}, address = {London}, issn = {0962-8452}, doi = {10.1098/rspb.2016.2235}, pages = {8}, year = {2017}, abstract = {Ancient DNA of extinct species from the Pleistocene and Holocene has provided valuable evolutionary insights. However, these are largely restricted to mammals and high latitudes because DNA preservation in warm climates is typically poor. In the tropics and subtropics, non-avian reptiles constitute a significant part of the fauna and little is known about the genetics of the many extinct reptiles from tropical islands. We have reconstructed the near-complete mitochondrial genome of an extinct giant tortoise from the Bahamas (Chelonoidis alburyorum) using an approximately 1000-year-old humerus from a water-filled sinkhole (blue hole) on Great Abaco Island. Phylogenetic and molecular clock analyses place this extinct species as closely related to Galapagos (C. niger complex) and Chaco tortoises (C. chilensis), and provide evidence for repeated overseas dispersal in this tortoise group. The ancestors of extant Chelonoidis species arrived in South America from Africa only after the opening of the Atlantic Ocean and dispersed from there to the Caribbean and the Galapagos Islands. Our results also suggest that the anoxic, thermally buffered environment of blue holes may enhance DNA preservation, and thus are opening a window for better understanding evolution and population history of extinct tropical species, which would likely still exist without human impact.}, language = {en} } @phdthesis{Kersting2017, author = {Kersting, Sebastian}, title = {Isothermal nucleic acid amplification for the detection of infectious pathogens}, pages = {215}, year = {2017}, language = {en} } @misc{KleineVehnSauer2017, author = {Kleine-Vehn, J{\"u}rgen and Sauer, Michael}, title = {Preface}, series = {Plant Hormones: Methods and Protocols}, volume = {1497}, journal = {Plant Hormones: Methods and Protocols}, editor = {Kleine-Vehn, J{\"u}rgen and Sauer, Michael}, edition = {3}, publisher = {Springer}, address = {New York}, isbn = {978-1-4939-6469-7}, issn = {1064-3745}, doi = {10.1007/978-1-4939-6469-7}, pages = {V -- V}, year = {2017}, language = {en} } @article{KloseRolkeBaumann2017, author = {Klose, Sascha Peter and Rolke, Daniel and Baumann, Otto}, title = {Morphogenesis of honeybee hypopharyngeal gland during pupal development}, series = {Frontiers in zoology}, volume = {14}, journal = {Frontiers in zoology}, publisher = {BioMed Central}, address = {London}, issn = {1742-9994}, doi = {10.1186/s12983-017-0207-z}, year = {2017}, abstract = {Background The hypopharyngeal gland of worker bees contributes to the production of the royal jelly fed to queens and larvae. The gland consists of thousands of two-cell units that are composed of a secretory cell and a duct cell and that are arranged in sets of about 12 around a long collecting duct. Results By fluorescent staining, we have examined the morphogenesis of the hypopharyngeal gland during pupal life, from a saccule lined by a pseudostratified epithelium to the elaborate organ of adult worker bees. The hypopharyngeal gland develops as follows. (1) Cell proliferation occurs during the first day of pupal life in the hypopharyngeal gland primordium. (2) Subsequently, the epithelium becomes organized into rosette-like units of three cells. Two of these will become the secretory cell and the duct cell of the adult secretory units; the third cell contributes only temporarily to the development of the secretory units and is eliminated by apoptosis in the second half of pupal life. (3) The three-cell units of flask-shaped cells undergo complex changes in cell morphology. Thus, by mid-pupal stage, the gland is structurally similar to the adult hypopharyngeal gland. (4) Concomitantly, the prospective secretory cell attains its characteristic subcellular organization by the invagination of a small patch of apical membrane domain, its extension to a tube of about 100 μm in length (termed a canaliculus), and the expansion of the tube to a diameter of about 3 μm. (6) Finally, the canaliculus-associated F-actin system becomes reorganized into rings of bundled actin filaments that are positioned at regular distances along the membrane tube. Conclusions The morphogenesis of the secretory units in the hypopharyngeal gland of the worker bee seems to be based on a developmental program that is conserved, with slight modification, among insects for the production of dermal glands. Elaboration of the secretory cell as a unicellular seamless epithelial tube occurs by invagination of the apical membrane, its extension likely by targeted exocytosis and its expansion, and finally the reorganisation of the membrane-associated F-actin system. Our work is fundamental for future studies of environmental effects on hypopharyngeal gland morphology and development.}, language = {en} } @phdthesis{Knecht2017, author = {Knecht, Volker}, title = {Modeling Biomolecular Association}, school = {Universit{\"a}t Potsdam}, pages = {297}, year = {2017}, language = {en} } @article{KocJanuchtaHoefflerThomaetal.2017, author = {Koc-Januchta, Marta and H{\"o}ffler, Tim and Thoma, Gun-Brit and Prechtl, Helmut and Leutner, Detlev}, title = {Visualizers versus verbalizers}, series = {Computers in human behavior}, volume = {68}, journal = {Computers in human behavior}, publisher = {Elsevier}, address = {Oxford}, issn = {0747-5632}, doi = {10.1016/j.chb.2016.11.028}, pages = {170 -- 179}, year = {2017}, abstract = {This study was conducted in order to examine the differences between visualizers and verbalizers in the way they gaze at pictures and texts while learning. Using a collection of questionnaires, college students were classified according to their visual or verbal cognitive style and were asked to learn about two different, in terms of subject and type of knowledge, topics by means of text-picture combinations. Eye-tracking was used to investigate their gaze behavior. The results show that visualizers spent significantly more time inspecting pictures than verbalizers, while verbalizers spent more time inspecting texts. Results also suggest that both visualizers' and verbalizers' way of learning is active but mostly within areas providing the source of information in line with their cognitive style (pictures or text). Verbalizers tended to enter non-informative, irrelevant areas of pictures sooner than visualizers. The comparison of learning outcomes showed that the group of visualizers achieved better results than the group of verbalizers on a comprehension test.}, language = {en} } @misc{KocyanWilandSzymanska2017, author = {Kocyan, Alexander and Wiland-Szymanska, Justyna}, title = {A new name and a new combination for Friedmannia nom. illeg. (Hypoxidaceae)}, series = {Phytotaxa : a rapid international journal for accelerating the publication of botanical taxonomy}, volume = {291}, journal = {Phytotaxa : a rapid international journal for accelerating the publication of botanical taxonomy}, number = {3}, publisher = {Magnolia Press}, address = {Auckland}, issn = {1179-3155}, doi = {10.11646/phytotaxa.291.3.10}, pages = {239 -- 239}, year = {2017}, abstract = {Recently, Kocyan \& Wiland-Szymańska (2016) have published a thorough research article on one of the outstanding members of the family Hypoxidaceae on the Seychelles, which resulted in the raise of a new genus (Friedmannia Kocyan \& Wiland-Szymańska 2016: 60) to accommodate the former Curculigo seychellensis Bojer ex Baker (1877: 368). However, it has turned out that the name Friedmannia Chantanachat \& Bold (1962: 45) already exists in literature for a green alga, which renders the new hypoxid genus illegitimate (Melbourne Code; McNeill et al. 2012). Therefore, we assign a new generic epithet to Curculigo seychellensis.}, language = {en} } @article{KolkNaafWulf2017, author = {Kolk, Jens and Naaf, Tobias and Wulf, Monika}, title = {Paying the colonization credit}, series = {Biodiversity and conservation}, volume = {26}, journal = {Biodiversity and conservation}, publisher = {Springer}, address = {Dordrecht}, issn = {0960-3115}, doi = {10.1007/s10531-016-1271-y}, pages = {735 -- 755}, year = {2017}, abstract = {Massive historical land cover changes in the Central European lowlands have resulted in a forest distribution that now comprises small remnants of ancient forests and more recently established post-agricultural forests. Here, land-use history is considered a key driver of recent herb-layer community changes, where an extinction debt in ancient forest remnants and/or a colonization credit in post-agricultural forests are being paid over time. On a regional scale, these payments should in theory lead toward a convergence in species richness between ancient and post-agricultural forests over time. In this study, we tested this assumption with a resurvey of 117 semi-permanent plots in the well-studied deciduous forests of the Prignitz region (Brandenburg, NE Germany), where we knew that the plant communities of post-agricultural stands exhibit a colonization credit while the extinction debt in ancient stands has largely been paid. We compared changes in the species richness of all herb layer species, forest specialists and ancient forest indicator species between ancient and post-agricultural stands with linear mixed effect models and determined the influence of patch connectivity on the magnitude of species richness changes. Species richness increased overall, but the richness of forest specialists increased significantly more in post-agricultural stands and was positively influenced by higher patch connectivity, indicating a convergence in species richness between the ancient and postagricultural stands. Furthermore, the richness of ancient forest indicator species only increased significantly in post-agricultural stands. For the first time, we were able to verify a gradual payment of the colonization credit in post-agricultural forest stands using a comparison of actual changes in temporal species richness.}, language = {en} } @article{KoussoroplisSchwarzenbergerWacker2017, author = {Koussoroplis, Apostolos-Manuel and Schwarzenberger, Anke and Wacker, Alexander}, title = {Diet quality determines lipase gene expression and lipase/esterase activity in Daphnia pulex}, series = {Biology open : BiO}, volume = {6}, journal = {Biology open : BiO}, publisher = {The company of Biologists}, address = {Cambridge}, doi = {10.1242/bio.022046}, pages = {210 -- 216}, year = {2017}, abstract = {We studied the short- (12 h) and long-term (144 h) response of Daphnia pulex lipases to quality shifts in diets consisting of different mixtures of the green alga Scenedesmus with the cyanobacterium Synechococcus, two species with contrasting lipid compositions. The lipase/esterase activity in both the gut and the body tissues had fast responses to the diet shift and increased with higher dietary contributions of Synechococcus. When screening the Daphnia genome for TAG lipases, we discovered a large gene-family expansion of these enzymes. We used a subset of eight genes for mRNA expression analyses and distinguished between influences of time and diet on the observed gene expression patterns. We identified five diet-responsive lipases of which three showed a sophisticated short- and long-term pattern of expression in response to small changes in food-quality. Furthermore, the gene expression of one of the lipases was strongly correlated to lipase/esterase activity in the gut suggesting its potentially major role in digestion. These findings demonstrate that the lipid-related enzymatic machinery of D. pulex is finely tuned to diet and might constitute an important mechanism of physiological adaptation in nutritionally complex environments.}, language = {en} } @misc{KozielHermanussenGomulaetal.2017, author = {Koziel, Slawomir and Hermanussen, Michael and Gomula, Alexandra and Swanson, James and Kaczmarek, Maria and El-Shabrawi, Mortada and Elhusseini, Mona and Satake, Takashi and Martinovic Klaric, Irena and Scheffler, Christiane and Morkuniene, Ruta and Godina, Elena and Sasa, Missoni and Tutkuviene, Janina and Siniarska, Anna and Nieczuja-Dwojacka, Joanna and Nunez, Javier and Groth, Detlef and Barbieri, Davide}, title = {Adolescence - a Transition to Adulthood Proceedings of the 24th Aschauer Soiree, held at Jurata, Poland, November 5th 2016}, series = {Pediatric Endocrinology Reviews}, volume = {14}, journal = {Pediatric Endocrinology Reviews}, number = {3}, publisher = {Medical Media}, address = {Netanya}, issn = {1565-4753}, pages = {326 -- 334}, year = {2017}, abstract = {Eighteen scientists met at Jurata, Poland, to discuss various aspects of the transition from adolescence to adulthood. This transition is a delicate period facing complex interactions between the adolescents and the social group they belong to. Social identity, group identification and identity signalling, but also stress affecting basal salivary cortisol rhythms, hypertension, inappropriate nutrition causing latent and manifest obesity, moreover, in developing and under-developed countries, parasitosis causing anaemia thereby impairing growth and development, are issues to be dealt with during this period of the human development. In addition, some new aspects of the association between weight, height and head circumference in the newborns were discussed, as well as intrauterine head growth and head circumference as health risk indicators.}, language = {en} } @misc{KramerLenhard2017, author = {Kramer, Elena M. and Lenhard, Michael}, title = {Shape and form in plant development}, series = {Seminars in cell \& developmental biology}, volume = {79}, journal = {Seminars in cell \& developmental biology}, publisher = {Elsevier}, address = {London}, issn = {1084-9521}, doi = {10.1016/j.semcdb.2017.11.004}, pages = {1 -- 2}, year = {2017}, language = {en} } @phdthesis{Kruse2017, author = {Kruse, Stefan}, title = {Larix treeline dynamics in northern Siberia inferred from population genetics and individual-based modelling}, school = {Universit{\"a}t Potsdam}, pages = {181}, year = {2017}, language = {en} } @phdthesis{Kubsch2017, author = {Kubsch, Bastian}, title = {Phase-specific fusion between biomembranes using SNARE mimetics}, school = {Universit{\"a}t Potsdam}, pages = {95}, year = {2017}, language = {en} }