@article{NaliboffGlerumBruneetal.2020, author = {Naliboff, John B. and Glerum, Anne and Brune, Sascha and P{\´e}ron-Pinvidic, G. and Wrona, Thilo}, title = {Development of 3-D rift heterogeneity through fault network evolution}, series = {Geophysical Research Letters}, volume = {47}, journal = {Geophysical Research Letters}, number = {13}, publisher = {John Wiley \& Sons, Inc.}, address = {New Jersey}, pages = {11}, year = {2020}, abstract = {Observations of rift and rifted margin architecture suggest that significant spatial and temporal structural heterogeneity develops during the multiphase evolution of continental rifting. Inheritance is often invoked to explain this heterogeneity, such as preexisting anisotropies in rock composition, rheology, and deformation. Here, we use high-resolution 3-D thermal-mechanical numerical models of continental extension to demonstrate that rift-parallel heterogeneity may develop solely through fault network evolution during the transition from distributed to localized deformation. In our models, the initial phase of distributed normal faulting is seeded through randomized initial strength perturbations in an otherwise laterally homogeneous lithosphere extending at a constant rate. Continued extension localizes deformation onto lithosphere-scale faults, which are laterally offset by tens of km and discontinuous along-strike. These results demonstrate that rift- and margin-parallel heterogeneity of large-scale fault patterns may in-part be a natural byproduct of fault network coalescence.}, language = {en} } @article{ZimmermannDeVeaughGeissToennisetal.2020, author = {Zimmermann, Malte and De Veaugh-Geiss, Joseph P. and T{\"o}nnis, Swantje and Onea, Edgar}, title = {(Non-)exhaustivity in focus partitioning across languages}, series = {Approaches to Hungarian}, volume = {16}, journal = {Approaches to Hungarian}, publisher = {John Benjamins}, address = {Amsterdam}, pages = {24}, year = {2020}, abstract = {We present novel experimental evidence on the availability and the status of exhaustivity inferences with focus partitioning in German, English, and Hungarian. Results suggest that German and English focus-background clefts and Hungarian focus share important properties, ({\´E}. Kiss 1998, 1999; Szabolcsi 1994; Percus 1997; Onea \& Beaver 2009). Those constructions are anaphoric devices triggering an existence presupposition. EXH-inferences are not obligatory in such constructions in English, German, or Hungarian, against some previous literature (Percus 1997; B{\"u}ring \& Križ 2013; {\´E}. Kiss 1998), but in line with pragmatic analyses of EXH-inferences in clefts (Horn 1981, 2016; Pollard \& Yasavul 2016). The cross-linguistic differences in the distribution of EXH-inferences are attributed to properties of the Hungarian number marking system.}, language = {en} } @article{HegerNiklesJacobs2018, author = {Heger, Tina and Nikles, Gabriele and Jacobs, Brooke S.}, title = {Differentiation in native as well as introduced ranges}, series = {AoB PLANTS}, volume = {10}, journal = {AoB PLANTS}, number = {1}, publisher = {Oxford Univ. Press}, address = {Oxford}, issn = {2041-2851}, doi = {10.1093/aobpla/ply009}, pages = {12}, year = {2018}, abstract = {Germination, a crucial phase in the life cycle of a plant, can be significantly influenced by competition and facilitation. The aim of this study was to test whether differences in cover of surrounding vegetation can lead to population differentiation in germination behaviour of an annual grassland species, and if so, whether such a differentiation can be found in the native as well as in the introduced range. We used maternal progeny of Erodium cicutarium previously propagated under uniform conditions that had been collected in multiple populations in the native and two introduced ranges, in populations representing extremes in terms of mean and variability of the cover of surrounding vegetation. In the first experiment, we tested the effect of germination temperature and mean cover at the source site on germination, and found interlinked effects of these factors. In seeds from one of the introduced ranges (California), we found indication for a 2-fold dormancy, hindering germination at high temperatures even if physical dormancy was broken and water was available. This behaviour was less strong in high cover populations, indicating cross-generational facilitating effects of dense vegetation. In the second experiment, we tested whether spatial variation in cover of surrounding vegetation has an effect on the proportion of dormant seeds. Contrary to our expectations, we found that across source regions, high variance in cover was associated with higher proportions of seeds germinating directly after storage. In all three regions, germination seemed to match the local environment in terms of climate and vegetation cover. We suggest that this is due to a combined effect of introduction of preadapted genotypes and local evolutionary processes.}, language = {en} } @article{HesseKlierSgarzietal.2018, author = {Hesse, Julia and Klier, Dennis Tobias and Sgarzi, Massimo and Nsubuga, Anne and Bauer, Christoph and Grenzer, Joerg and H{\"u}bner, Rene and Wislicenus, Marcus and Joshi, Tanmaya and Kumke, Michael Uwe and Stephan, Holger}, title = {Rapid Synthesis of Sub-10nm Hexagonal NaYF4-Based Upconverting Nanoparticles using Therminol((R))66}, series = {ChemistryOpen : including thesis treasury}, volume = {7}, journal = {ChemistryOpen : including thesis treasury}, number = {2}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {2191-1363}, doi = {10.1002/open.201700186}, pages = {159 -- 168}, year = {2018}, abstract = {We report a simple one-pot method for the rapid preparation of sub-10nm pure hexagonal (-phase) NaYF4-based upconverting nanoparticles (UCNPs). Using Therminol((R))66 as a co-solvent, monodisperse UCNPs could be obtained in unusually short reaction times. By varying the reaction time and reaction temperature, it was possible to control precisely the particle size and crystalline phase of the UCNPs. The upconversion (UC) luminescence properties of the nanocrystals were tuned by varying the concentrations of the dopants (Nd3+ and Yb3+ sensitizer ions and Er3+ activator ions). The size and phase-purity of the as-synthesized core and core-shell nanocrystals were assessed by using complementary transmission electron microscopy, dynamic light scattering, X-ray diffraction, and small-angle X-ray scattering studies. In-depth photophysical evaluation of the UCNPs was pursued by using steady-state and time-resolved luminescence spectroscopy. An enhancement in the UC intensity was observed if the nanocrystals, doped with optimized concentrations of lanthanide sensitizer/activator ions, were further coated with an inert/active shell. This was attributed to the suppression of surface-related luminescence quenching effects.}, language = {en} } @article{KaechMatheHubertDennisetal.2018, author = {K{\"a}ch, Heidi and Mathe-Hubert, Hugo and Dennis, Alice B. and Vorburger, Christoph}, title = {Rapid evolution of symbiont-mediated resistance compromises biological control of aphids by parasitoids}, series = {Evolutionary applications}, volume = {11}, journal = {Evolutionary applications}, number = {2}, publisher = {Wiley}, address = {Hoboken}, issn = {1752-4571}, doi = {10.1111/eva.12532}, pages = {220 -- 230}, year = {2018}, abstract = {There is growing interest in biological control as a sustainable and environmentally friendly way to control pest insects. Aphids are among the most detrimental agricultural pests worldwide, and parasitoid wasps are frequently employed for their control. The use of asexual parasitoids may improve the effectiveness of biological control because only females kill hosts and because asexual populations have a higher growth rate than sexuals. However, asexuals may have a reduced capacity to track evolutionary change in their host populations. We used a factorial experiment to compare the ability of sexual and asexual populations of the parasitoid Lysiphlebus fabarum to control caged populations of black bean aphids (Aphis fabae) of high and low clonal diversity. The aphids came from a natural population, and one-third of the aphid clones harbored Hamiltonella defensa, a heritable bacterial endosymbiont that increases resistance to parasitoids. We followed aphid and parasitoid population dynamics for 3months but found no evidence that the reproductive mode of parasitoids affected their effectiveness as biocontrol agents, independent of host clonal diversity. Parasitoids failed to control aphids in most cases, because their introduction resulted in strong selection for clones protected by H.defensa. The increasingly resistant aphid populations escaped control by parasitoids, and we even observed parasitoid extinctions in many cages. The rapid evolution of symbiont-conferred resistance in turn imposed selection on parasitoids. In cages where asexual parasitoids persisted until the end of the experiment, they became dominated by a single genotype able to overcome the protection provided by H.defensa. Thus, there was evidence for parasitoid counteradaptation, but it was generally too slow for parasitoids to regain control over aphid populations. It appears that when pest aphids possess defensive symbionts, the presence of parasitoid genotypes able to overcome symbiont-conferred resistance is more important for biocontrol success than their reproductive mode.}, language = {en} } @article{ShiJoshiTielboergeretal.2018, author = {Shi, Jun and Joshi, Jasmin Radha and Tielboerger, Katja and Verhoeven, Koen J. F. and Macel, Mirka}, title = {Costs and benefits of admixture between foreign genotypes and local populations in the field}, series = {Ecology and evolution}, volume = {8}, journal = {Ecology and evolution}, number = {7}, publisher = {Wiley}, address = {Hoboken}, issn = {2045-7758}, doi = {10.1002/ece3.3946}, pages = {3675 -- 3684}, year = {2018}, abstract = {Admixture is the hybridization between populations within one species. It can increase plant fitness and population viability by alleviating inbreeding depression and increasing genetic diversity. However, populations are often adapted to their local environments and admixture with distant populations could break down local adaptation by diluting the locally adapted genomes. Thus, admixed genotypes might be selected against and be outcompeted by locally adapted genotypes in the local environments. To investigate the costs and benefits of admixture, we compared the performance of admixed and within-population F1 and F2 generations of the European plant Lythrum salicaria in a reciprocal transplant experiment at three European field sites over a 2-year period. Despite strong differences between site and plant populations for most of the measured traits, including herbivory, we found limited evidence for local adaptation. The effects of admixture depended on experimental site and plant population, and were positive for some traits. Plant growth and fruit production of some populations increased in admixed offspring and this was strongest with larger parental distances. These effects were only detected in two of our three sites. Our results show that, in the absence of local adaptation, admixture may boost plant performance, and that this is particularly apparent in stressful environments. We suggest that admixture between foreign and local genotypes can potentially be considered in nature conservation to restore populations and/or increase population viability, especially in small inbred or maladapted populations.}, language = {en} } @article{TotzEliseevPetrietal.2018, author = {Totz, Sonja Juliana and Eliseev, Alexey V. and Petri, Stefan and Flechsig, Michael and Caesar, Levke and Petoukhov, Vladimir and Coumou, Dim}, title = {The dynamical core of the Aeolus 1.0 statistical-dynamical atmosphere model}, series = {Geoscientific model development : an interactive open access journal of the European Geosciences Union}, volume = {11}, journal = {Geoscientific model development : an interactive open access journal of the European Geosciences Union}, number = {2}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {1991-959X}, doi = {10.5194/gmd-11-665-2018}, pages = {665 -- 679}, year = {2018}, abstract = {Here, we present novel equations for the large-scale zonal-mean wind as well as those for planetary waves. Together with synoptic parameterization (as presented by Coumou et al., 2011), these form the mathematical description of the dynamical core of Aeolus 1.0. The regions of high azonal wind velocities (planetary waves) are accurately captured for all validation experiments. The zonal-mean zonal wind and the integrated lower troposphere mass flux show good results in particular in the Northern Hemisphere. In the Southern Hemisphere, the model tends to produce too-weak zonal-mean zonal winds and a too-narrow Hadley circulation. We discuss possible reasons for these model biases as well as planned future model improvements and applications.}, language = {en} } @article{WeithoffTaubeBolius2017, author = {Weithoff, Guntram and Taube, Anne and Bolius, Sarah}, title = {The invasion success of the cyanobacterium Cylindrospermopsis raciborskii in experimental mesocosms}, series = {Aquatic Invasions}, volume = {12}, journal = {Aquatic Invasions}, publisher = {Regional Euro-Asian Biological Invasions centre-reabic}, address = {Helsinki}, issn = {1798-6540}, doi = {10.3391/ai.2017.12.3.07}, pages = {333 -- 341}, year = {2017}, abstract = {The potentially toxic, invasive cyanobacterium Cylindrospermopsis raciborskii, originating from sub-tropical regions, has spread into temperate climate zones in almost all continents. Potential factors in its success are temperature, light and nutrient levels. Grazing losses through zooplankton have been measured in the laboratory but are typically not regarded as a factor in (failed) invasion success. In some potentially suitable lakes, C. raciborskii has never been found, although it is present in water bodies close by. Therefore, we tested the invasive potential of three different isolates introduced into natural plankton communities using laboratory mesocosm experiments under three grazing levels: ambient zooplankton densities, removal of large species using 100 mu m mesh and a ca. doubling of large species. Three C. raciborskii isolates originating from the same geographic region (North-East Germany) were added separately to the four replicates of each treatment and kept in semi-continuous cultures for 21 days. Two isolates disappeared from the mesocosms and were also not viable in filtered lake water indicating that the lake water itself or the switch from culture medium to lake water led to the decay of the inoculated C. raciborskii. Only one out of the three isolates persisted in the plankton communities at a rather low level and only in the treatment without larger zooplankton. This result demonstrates that under potentially suitable environmental conditions, top-down control from zooplankton might hamper the establishment of C. raciborskii. Non-metric multidimensional scaling showed distinct variation in resident phytoplankton communities between the different grazing levels, thus differential grazing impact shaped the resident community in different ways allowing C. raciborskii only to invade under competitive (= low grazing pressure) conditions. Furthermore, even after invasion failure, the temporary presence of C. raciborskii influenced the phytoplankton community.}, language = {en} } @article{VaterMoritzRoepke2018, author = {Vater, Aline and Moritz, Steffen and Roepke, Stefan}, title = {Does a narcissism epidemic exist in modern western societies?}, series = {PLOS ONE}, volume = {13}, journal = {PLOS ONE}, number = {1}, publisher = {Public Library of Science}, address = {San Fransisco}, issn = {1932-6203}, doi = {10.1371/journal.pone.0188287}, pages = {16}, year = {2018}, abstract = {Narcissism scores are higher in individualistic cultures compared with more collectivistic cultures. However, the impact of sociocultural factors on narcissism and self-esteem has not been well described. Germany was formerly divided into two different social systems, each with distinct economic, political and national cultures, and was reunified in 1989/90. Between 1949 and 1989/90, West Germany had an individualistic culture, whereas East Germany had a more collectivistic culture. The German reunification provides an exceptional opportunity to investigate the impact of sociocultural and generational differences on narcissism and self-esteem. In this study, we used an anonymous online survey to assess grandiose narcissism with the Narcissistic Personality Inventory (NPI) and the Pathological Narcissism Inventory (PNI) to assess grandiose and vulnerable aspects of narcissism, and self-esteem with the Rosenberg Self-Esteem Scale (RSE) in 1,025 German individuals. Data were analyzed according to age and place of birth. Our results showed that grandiose narcissism was higher and self-esteem was lower in individuals who grew up in former West Germany compared with former East Germany. Further analyses indicated no significant differences in grandiose narcissism, vulnerable narcissism or self-esteem in individuals that entered school after the German reunification (≤ 5 years of age in 1989). In the middle age cohort (6-18 years of age in 1989), significant differences in vulnerable narcissism, grandiose narcissism and self-esteem were observed. In the oldest age cohort (> 19 years of age in 1989), significant differences were only found in one of the two scales assessing grandiose narcissism (NPI). Our data provides empirical evidence that sociocultural factors are associated with differences in narcissism and self-esteem.}, language = {en} } @article{WolfGillesPeusetal.2018, author = {Wolf, Isabell Ann-Cathrin and Gilles, Maria and Peus, Verena and Scharnholz, Barbara and Seibert, Julia and Jennen-Steinmetz, Christine and Krumm, Bertram and Rietschel, Marcella and Deuschle, Michael and Laucht, Manfred}, title = {Impact of prenatal stress on mother-infant dyadic behavior during the still-face paradigm}, series = {Borderline Personality Disorder and Emotion Dysregulation : the official journal of the National Education Alliance for Borderline Personality Disorder (NEA.BPD) and Dachverband Dialektisch Behaviorale Therapie (DDBT)}, volume = {5}, journal = {Borderline Personality Disorder and Emotion Dysregulation : the official journal of the National Education Alliance for Borderline Personality Disorder (NEA.BPD) and Dachverband Dialektisch Behaviorale Therapie (DDBT)}, publisher = {BioMed Central}, address = {London}, issn = {2051-6673}, doi = {10.1186/s40479-018-0078-8}, pages = {13}, year = {2018}, abstract = {Background: Mother-infant interaction provides important training for the infant's ability to cope with stress and the development of resilience. Prenatal stress (PS) and its impact on the offspring's development have long been a focus of stress research, with studies highlighting both harmful and beneficial effects. The aim of the current study was to examine the possible influence of both psychological stress and hypothalamic-pituitary-adrenal (HPA) axis activity during pregnancy with mother-child dyadic behavior following stress exposure. Methods: The behavior of 164 mother-infant dyads during the still-face situation was filmed at six months postpartum and coded into three dyadic patterns: 1) both positive, 2) infant protesting-mother positive, and 3) infant protesting-mother negative. PS exposure was assessed prenatally according to psychological measures (i.e., psychopathological, perceived and psychosocial PS; n = 164) and HPA axis activity measures (maternal salivary cortisol, i.e., cortisol decline and area under the curve with respect to ground (AUCg); n = 134). Results: Mother-infant dyads in both the high- and low-stress groups showed decreasing positive and increasing negative dyadic behavior in the reunion episode, which is associated with the well-known "still-face" and "carry-over" effect. Furthermore, mother-infant dyads with higher psychosocial PS exhibited significantly more positive dyadic behavior than the low psychosocial PS group in the first play episode, but not in the reunion episode. Similarly, mother-infant dyads with high HPA axis activity (i.e. high AUCg) but steeper diurnal cortisol decline (i.e. cortisol decline) displayed significantly less negative behavior in the reunion episode than dyads with low HPA axis activity. No significant results were found for psychopathological stress and perceived stress. Conclusions: The results suggest a beneficial effect of higher psychosocial PS and higher prenatal maternal HPA axis activity in late gestation, which is in line with "stress inoculation" theories.}, language = {en} } @article{BukowskiSchittkoPetermann2018, author = {Bukowski, Alexandra R. and Schittko, Conrad and Petermann, Jana S.}, title = {The strength of negative plant-soil feedback increases from the intraspecific to the interspecific and the functional group level}, series = {Ecology and evolution}, volume = {8}, journal = {Ecology and evolution}, number = {4}, publisher = {Wiley}, address = {Hoboken}, issn = {2045-7758}, doi = {10.1002/ece3.3755}, pages = {2280 -- 2289}, year = {2018}, abstract = {One of the processes that may play a key role in plant species coexistence and ecosystem functioning is plant-soil feedback, the effect of plants on associated soil communities and the resulting feedback on plant performance. Plant-soil feedback at the interspecific level (comparing growth on own soil with growth on soil from different species) has been studied extensively, while plant-soil feedback at the intraspecific level (comparing growth on own soil with growth on soil from different accessions within a species) has only recently gained attention. Very few studies have investigated the direction and strength of feedback among different taxonomic levels, and initial results have been inconclusive, discussing phylogeny, and morphology as possible determinants. To test our hypotheses that the strength of negative feedback on plant performance increases with increasing taxonomic level and that this relationship is explained by morphological similarities, we conducted a greenhouse experiment using species assigned to three taxonomic levels (intraspecific, interspecific, and functional group level). We measured certain fitness-related aboveground traits and used them along literature-derived traits to determine the influence of morphological similarities on the strength and direction of the feedback. We found that the average strength of negative feedback increased from the intraspecific over the interspecific to the functional group level. However, individual accessions and species differed in the direction and strength of the feedback. None of our results could be explained by morphological dissimilarities or individual traits. Synthesis. Our results indicate that negative plant-soil feedback is stronger if the involved plants belong to more distantly related species. We conclude that the taxonomic level is an important factor in the maintenance of plant coexistence with plant-soil feedback as a potential stabilizing mechanism and should be addressed explicitly in coexistence research, while the traits considered here seem to play a minor role.}, language = {en} } @article{KuecuekgoezeLeimkuehler2018, author = {K{\"u}{\c{c}}{\"u}kg{\"o}ze, G{\"o}khan and Leimk{\"u}hler, Silke}, title = {Direct comparison of the four aldehyde oxidase enzymes present in mouse gives insight into their substrate specificities}, series = {PLOS ONE}, volume = {13}, journal = {PLOS ONE}, number = {1}, publisher = {Public Library of Science}, address = {San Fransisco}, issn = {1932-6203}, doi = {10.1371/journal.pone.0191819}, pages = {20}, year = {2018}, abstract = {Mammalian aldehyde oxidases (AOXs) are molybdo-flavoenzymes which are present in many tissues in various mammalian species, including humans and rodents. Different species contain a different number of AOX isoforms. In particular, the reasons why mammals other than humans express a multiplicity of tissue-specific AOX enzymes is unknown. In mouse, the isoforms mAOX1, mAOX3, mAOX4 and mAOX2 are present. We previously established a codon-optimized heterologous expression systems for the mAOX1-4 isoforms in Escherichia coli that gives yield to sufficient amounts of active protein for kinetic characterizations and sets the basis in this study for site-directed mutagenesis and structure-function studies. A direct and simultaneous comparison of the enzymatic properties and characteristics of the four enzymes on a larger number of substrates has never been performed. Here, thirty different structurally related aromatic, aliphatic and N-heterocyclic compounds were used as substrates, and the kinetic parameters of all four mAOX enzymes were directly compared. The results show that especially mAOX4 displays a higher substrate selectivity, while no major differences between mAOX1, mAOX2 and mAOX3 were identified. Generally, mAOX1 was the enzyme with the highest catalytic turnover for most substrates. To understand the factors that contribute to the substrate specificity of mAOX4, site-directed mutagenesis was applied to substitute amino acids in the substrate-binding funnel by the ones present in mAOX1, mAOX3, and mAOX2. An increase in activity was obtained by the amino acid exchange M1088V in the active site identified to be specific for mAOX4, to the amino acid identified in mAOX3.}, language = {en} } @article{LaraNitzeGrosseetal.2018, author = {Lara, Mark J. and Nitze, Ingmar and Grosse, Guido and Martin, Philip and McGuire, A. David}, title = {Reduced arctic tundra productivity linked with landform and climate change interactions}, series = {Scientific reports}, volume = {8}, journal = {Scientific reports}, publisher = {Nature Publ. Group}, address = {London}, issn = {2045-2322}, doi = {10.1038/s41598-018-20692-8}, pages = {10}, year = {2018}, abstract = {Arctic tundra ecosystems have experienced unprecedented change associated with climate warming over recent decades. Across the Pan-Arctic, vegetation productivity and surface greenness have trended positively over the period of satellite observation. However, since 2011 these trends have slowed considerably, showing signs of browning in many regions. It is unclear what factors are driving this change and which regions/landforms will be most sensitive to future browning. Here we provide evidence linking decadal patterns in arctic greening and browning with regional climate change and local permafrost-driven landscape heterogeneity. We analyzed the spatial variability of decadal-scale trends in surface greenness across the Arctic Coastal Plain of northern Alaska (similar to 60,000 km(2)) using the Landsat archive (1999-2014), in combination with novel 30 m classifications of polygonal tundra and regional watersheds, finding landscape heterogeneity and regional climate change to be the most important factors controlling historical greenness trends. Browning was linked to increased temperature and precipitation, with the exception of young landforms (developed following lake drainage), which will likely continue to green. Spatiotemporal model forecasting suggests carbon uptake potential to be reduced in response to warmer and/or wetter climatic conditions, potentially increasing the net loss of carbon to the atmosphere, at a greater degree than previously expected.}, language = {en} } @article{DaiMateGlebeetal.2018, author = {Dai, Xiaolin and Mate, Diana M. and Glebe, Ulrich and Garakani, Tayebeh Mirzaei and K{\"o}rner, Andrea and Schwaneberg, Ulrich and B{\"o}ker, Alexander}, title = {Sortase-mediated ligation of purely artificial building blocks}, series = {Polymers}, volume = {10}, journal = {Polymers}, number = {2}, publisher = {MDPI}, address = {Basel}, issn = {2073-4360}, doi = {10.3390/polym10020151}, pages = {13}, year = {2018}, abstract = {Sortase A (SrtA) from Staphylococcus aureus has been often used for ligating a protein with other natural or synthetic compounds in recent years. Here we show that SrtA-mediated ligation (SML) is universally applicable for the linkage of two purely artificial building blocks. Silica nanoparticles (NPs), poly(ethylene glycol) and poly(N-isopropyl acrylamide) are chosen as synthetic building blocks. As a proof of concept, NP-polymer, NP-NP, and polymer-polymer structures are formed by SrtA catalysis. Therefore, the building blocks are equipped with the recognition sequence needed for SrtA reaction-the conserved peptide LPETG-and a pentaglycine motif. The successful formation of the reaction products is shown by means of transmission electron microscopy (TEM), matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS), and dynamic light scattering (DLS). The sortase catalyzed linkage of artificial building blocks sets the stage for the development of a new approach to link synthetic structures in cases where their synthesis by established chemical methods is complicated.}, language = {en} } @article{KrapfMarinariMetzleretal.2018, author = {Krapf, Diego and Marinari, Enzo and Metzler, Ralf and Oshanin, Gleb and Xu, Xinran and Squarcini, Alessio}, title = {Power spectral density of a single Brownian trajectory}, series = {New journal of physics : the open-access journal for physics}, volume = {20}, journal = {New journal of physics : the open-access journal for physics}, publisher = {IOP Publ. Ltd.}, address = {Bristol}, issn = {1367-2630}, doi = {10.1088/1367-2630/aaa67c}, pages = {30}, year = {2018}, abstract = {The power spectral density (PSD) of any time-dependent stochastic processX (t) is ameaningful feature of its spectral content. In its text-book definition, the PSD is the Fourier transform of the covariance function of X-t over an infinitely large observation timeT, that is, it is defined as an ensemble-averaged property taken in the limitT -> infinity. Alegitimate question is what information on the PSD can be reliably obtained from single-trajectory experiments, if one goes beyond the standard definition and analyzes the PSD of a single trajectory recorded for a finite observation timeT. In quest for this answer, for a d-dimensional Brownian motion (BM) we calculate the probability density function of a single-trajectory PSD for arbitrary frequency f, finite observation time T and arbitrary number k of projections of the trajectory on different axes. We show analytically that the scaling exponent for the frequency-dependence of the PSD specific to an ensemble of BM trajectories can be already obtained from a single trajectory, while the numerical amplitude in the relation between the ensemble-averaged and single-trajectory PSDs is afluctuating property which varies from realization to realization. The distribution of this amplitude is calculated exactly and is discussed in detail. Our results are confirmed by numerical simulations and single-particle tracking experiments, with remarkably good agreement. In addition we consider a truncated Wiener representation of BM, and the case of a discrete-time lattice random walk. We highlight some differences in the behavior of a single-trajectory PSD for BM and for the two latter situations. The framework developed herein will allow for meaningful physical analysis of experimental stochastic trajectories.}, language = {en} } @article{GoodwinMuddClubb2018, author = {Goodwin, Guillaume C. H. and Mudd, Simon M. and Clubb, Fiona J.}, title = {Unsupervised detection of salt marsh platforms}, series = {Earth surface dynamics}, volume = {6}, journal = {Earth surface dynamics}, number = {1}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {2196-6311}, doi = {10.5194/esurf-6-239-2018}, pages = {239 -- 255}, year = {2018}, abstract = {Salt marshes filter pollutants, protect coastlines against storm surges, and sequester carbon, yet are under threat from sea level rise and anthropogenic modification. The sustained existence of the salt marsh ecosystem depends on the topographic evolution of marsh platforms. Quantifying marsh platform topography is vital for improving the management of these valuable landscapes. The determination of platform boundaries currently relies on supervised classification methods requiring near-infrared data to detect vegetation, or demands labour-intensive field surveys and digitisation. We propose a novel, unsupervised method to reproducibly isolate salt marsh scarps and platforms from a digital elevation model (DEM), referred to as Topographic Identification of Platforms (TIP). Field observations and numerical models show that salt marshes mature into subhorizontal platforms delineated by subvertical scarps. Based on this premise, we identify scarps as lines of local maxima on a slope raster, then fill landmasses from the scarps upward, thus isolating mature marsh platforms. We test the TIP method using lidar-derived DEMs from six salt marshes in England with varying tidal ranges and geometries, for which topographic platforms were manually isolated from tidal flats. Agreement between manual and unsupervised classification exceeds 94\% for DEM resolutions of 1 m, with all but one site maintaining an accuracy superior to 90\% for resolutions up to 3 m. For resolutions of 1 m, platforms detected with the TIP method are comparable in surface area to digitised platforms and have similar elevation distributions. We also find that our method allows for the accurate detection of local block failures as small as 3 times the DEM resolution. Detailed inspection reveals that although tidal creeks were digitised as part of the marsh platform, unsupervised classification categorises them as part of the tidal flat, causing an increase in false negatives and overall platform perimeter. This suggests our method may benefit from combination with existing creek detection algorithms. Fallen blocks and high tidal flat portions, associated with potential pioneer zones, can also lead to differences between our method and supervised mapping. Although pioneer zones prove difficult to classify using a topographic method, we suggest that these transition areas should be considered when analysing erosion and accretion processes, particularly in the case of incipient marsh platforms. Ultimately, we have shown that unsupervised classification of marsh platforms from high-resolution topography is possible and sufficient to monitor and analyse topographic evolution.}, language = {en} } @article{RoderHille2018, author = {Roder, Phillip and Hille, Carsten}, title = {Local tissue manipulation via a force- and pressure-controlled AFM micropipette for analysis of cellular processes}, series = {Scientific reports}, volume = {8}, journal = {Scientific reports}, publisher = {Nature Publ. Group}, address = {London}, issn = {2045-2322}, doi = {10.1038/s41598-018-24255-9}, pages = {9}, year = {2018}, abstract = {Local manipulation of complex tissues at the single-cell level is challenging and requires excellent sealing between the specimen and the micromanipulation device. Here, biological applications for a recently developed loading technique for a force-and pressure-controlled fluidic force microscope micropipette are described. This technique allows for the exact positioning and precise spatiotemporal control of liquid delivery. The feasibility of a local loading technique for tissue applications was investigated using two fluorescent dyes, with which local loading behaviour could be optically visualised. Thus, homogeneous intracellular distribution of CellTracker Red and accumulation of SYTO 9 Green within nuclei was realised in single cells of a tissue preparation. Subsequently, physiological micromanipulation experiments were performed. Salivary gland tissue was pre-incubated with the Ca2+-sensitive dye OGB-1. An intracellular Ca2+ rise was then initiated at the single-cell level by applying dopamine via micropipette. When pre-incubating tissue with the nitric oxide (NO)-sensitive dye DAF-FM, NO release and intercellular NO diffusion was observed after local application of the NO donor SNP. Finally, local micromanipulation of a well-defined area along irregularly shaped cell surfaces of complex biosystems was shown for the first time for the fluidic force microscope micropipette. Thus, this technique is a promising tool for the investigation of the spatiotemporal effects of locally applied substances in complex tissues.}, language = {en} } @article{Goychuk2018, author = {Goychuk, Igor}, title = {Sensing magnetic fields with magnetosensitive ion channels}, series = {Sensors}, volume = {18}, journal = {Sensors}, number = {3}, publisher = {MDPI}, address = {Basel}, issn = {1424-8220}, doi = {10.3390/s18030728}, pages = {19}, year = {2018}, abstract = {Magnetic nanoparticles are met across many biological species ranging from magnetosensitive bacteria, fishes, bees, bats, rats, birds, to humans. They can be both of biogenetic origin and due to environmental contamination, being either in paramagnetic or ferromagnetic state. The energy of such naturally occurring single-domain magnetic nanoparticles can reach up to 10-20 room k(B)T in the magnetic field of the Earth, which naturally led to supposition that they can serve as sensory elements in various animals. This work explores within a stochastic modeling framework a fascinating hypothesis of magnetosensitive ion channels with magnetic nanoparticles serving as sensory elements, especially, how realistic it is given a highly dissipative viscoelastic interior of living cells and typical sizes of nanoparticles possibly involved.}, language = {en} } @article{KayhanMatthesMarriottHaresignetal.2022, author = {Kayhan, Ezgi and Matthes, Daniel and Marriott Haresign, Ira and B{\´a}nki, Anna and Michel, Christine and Langeloh, Miriam and Wass, Sam and Hoehl, Stefanie}, title = {DEEP: A dual EEG pipeline for developmental hyperscanning studies}, series = {Developmental Cognitive Neuroscience}, volume = {54}, journal = {Developmental Cognitive Neuroscience}, publisher = {Elsevier}, address = {Amsterdam, Niederlande}, issn = {1878-9307}, doi = {10.1016/j.dcn.2022.101104}, pages = {1 -- 11}, year = {2022}, abstract = {Cutting-edge hyperscanning methods led to a paradigm shift in social neuroscience. It allowed researchers to measure dynamic mutual alignment of neural processes between two or more individuals in naturalistic contexts. The ever-growing interest in hyperscanning research calls for the development of transparent and validated data analysis methods to further advance the field. We have developed and tested a dual electroencephalography (EEG) analysis pipeline, namely DEEP. Following the preprocessing of the data, DEEP allows users to calculate Phase Locking Values (PLVs) and cross-frequency PLVs as indices of inter-brain phase alignment of dyads as well as time-frequency responses and EEG power for each participant. The pipeline also includes scripts to control for spurious correlations. Our goal is to contribute to open and reproducible science practices by making DEEP publicly available together with an example mother-infant EEG hyperscanning dataset.}, language = {en} } @article{CostaTomazdeSouzaAyzelHeistermann2020, author = {Costa Tomaz de Souza, Arthur and Ayzel, Georgy and Heistermann, Maik}, title = {Quantifying the location error of precipitation nowcasts}, series = {Advances in meteorology}, volume = {2020}, journal = {Advances in meteorology}, publisher = {Hindawi}, address = {London}, issn = {1687-9309}, doi = {10.1155/2020/8841913}, pages = {12}, year = {2020}, abstract = {In precipitation nowcasting, it is common to track the motion of precipitation in a sequence of weather radar images and to extrapolate this motion into the future. The total error of such a prediction consists of an error in the predicted location of a precipitation feature and an error in the change of precipitation intensity over lead time. So far, verification measures did not allow isolating the extent of location errors, making it difficult to specifically improve nowcast models with regard to location prediction. In this paper, we introduce a framework to directly quantify the location error. To that end, we detect and track scale-invariant precipitation features (corners) in radar images. We then consider these observed tracks as the true reference in order to evaluate the performance (or, inversely, the error) of any model that aims to predict the future location of a precipitation feature. Hence, the location error of a forecast at any lead time Delta t ahead of the forecast time t corresponds to the Euclidean distance between the observed and the predicted feature locations at t + Delta t. Based on this framework, we carried out a benchmarking case study using one year worth of weather radar composites of the German Weather Service. We evaluated the performance of four extrapolation models, two of which are based on the linear extrapolation of corner motion from t - 1 to t (LK-Lin1) and t - 4 to t (LK-Lin4) and the other two are based on the Dense Inverse Search (DIS) method: motion vectors obtained from DIS are used to predict feature locations by linear (DIS-Lin1) and Semi-Lagrangian extrapolation (DIS-Rot1). Of those four models, DIS-Lin1 and LK-Lin4 turned out to be the most skillful with regard to the prediction of feature location, while we also found that the model skill dramatically depends on the sinuosity of the observed tracks. The dataset of 376,125 detected feature tracks in 2016 is openly available to foster the improvement of location prediction in extrapolation-based nowcasting models.}, language = {en} }