@article{BatistaFigueiredoSantosGonzalezetal.2019, author = {Batista, Rita A. and Figueiredo, Duarte Dionisio and Santos-Gonzalez, Juan and K{\"o}hler, Claudia}, title = {Auxin regulates endosperm cellularization in Arabidopsis}, series = {Genes \& Development}, volume = {33}, journal = {Genes \& Development}, number = {7-8}, publisher = {Cold Spring Harbor Laboratory Press}, address = {Cold Spring Harbor, NY}, issn = {0890-9369}, doi = {10.1101/gad.316554.118}, pages = {466 -- 476}, year = {2019}, abstract = {The endosperm is an ephemeral tissue that nourishes the developing embryo, similar to the placenta in mammals. In most angiosperms, endosperm development starts as a syncytium, in which nuclear divisions are not followed by cytokinesis. The timing of endosperm cellularization largely varies between species, and the event triggering this transition remains unknown. Here we show that increased auxin biosynthesis in the endosperm prevents its cellularization, leading to seed arrest. Auxin-overproducing seeds phenocopy paternal-excess triploid seeds derived from hybridizations of diploid maternal plants with tetraploid fathers. Concurrently, auxin-related genes are strongly overexpressed in triploid seeds, correlating with increased auxin activity. Reducing auxin biosynthesis and signaling reestablishes endosperm cellularization in triploid seeds and restores their viability, highlighting a causal role of increased auxin in preventing endosperm cellularization. We propose that auxin determines the time of endosperm cellularization, and thereby uncovered a central role of auxin in establishing hybridization barriers in plants.}, language = {en} } @article{DaempflingMielkeKoellneretal.2022, author = {D{\"a}mpfling, Helge L. C. and Mielke, Christian and Koellner, Nicole and Lorenz, Melanie and Rogass, Christian and Altenberger, Uwe and Harlov, Daniel E. and Knoper, Michael}, title = {Automatic element and mineral detection in thin sections using hyperspectral transmittance imaging microscopy (HyperTIM)}, series = {European journal of mineralogy}, volume = {34}, journal = {European journal of mineralogy}, number = {3}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {0935-1221}, doi = {10.5194/ejm-34-275-2022}, pages = {275 -- 284}, year = {2022}, abstract = {In this study we present a novel method for the automatic detection of minerals and elements using hyperspectral transmittance imaging microscopy measurements of complete thin sections (HyperTIM). This is accomplished by using a hyperspectral camera system that operates in the visible and near-infrared (VNIR) range with a specifically designed sample holder, scanning setup, and a microscope lens. We utilize this method on a monazite ore thin section from Steenkampskraal (South Africa), which we analyzed for the rare earth element (REE)-bearing mineral monazite ((Ce,Nd,La)PO4), with high concentrations of Nd. The transmittance analyses with the hyperspectral VNIR camera can be used to identify REE minerals and Nd in thin sections. We propose a three-point band depth index, the Nd feature depth index (NdFD), and its related product the Nd band depth index (NdBDI), which enables automatic mineral detection and classification for the Nd-bearing monazites in thin sections. In combination with the average concentration of the relative Nd content, it permits a destruction-free, total concentration calculation for Nd across the entire thin section.}, language = {en} } @article{SchinkoethBrand2020, author = {Schink{\"o}th, Michaela and Brand, Ralf}, title = {Automatic associations and the affective valuation of exercise}, series = {German Journal of Exercise and Sport Research}, volume = {50}, journal = {German Journal of Exercise and Sport Research}, number = {654}, publisher = {Springer}, address = {Berlin ; Heidelberg}, issn = {2509-3150}, doi = {10.1007/s12662-020-00664-9}, pages = {366 -- 376}, year = {2020}, abstract = {The decision to exercise is not only bound to rational considerations but also automatic affective processes. The affective-reflective theory of physical inactivity and exercise (ART) proposes a theoretical framework for explaining how the automatic affective process (type‑1 process) will influence exercise behavior, i.e., through the automatic activation of exercise-related associations and a subsequent affective valuation of exercise. This study aimed to empirically test this assumption of the ART with data from 69 study participants. A single-measurement study, including within-subject experimental variation, was conducted. Automatic associations with exercise were first measured with a single-target implicit association test. The somato-affective core of the participants' automatic valuation of exercise-related pictures was then assessed via heart rate variability (HRV) analysis, and the affective valence of the valuation was tested with a facial expression (FE; smile and frown) task. Exercise behavior was assessed via self-report. Multiple regression (path) analysis revealed that automatic associations predicted HRV reactivity (β = -0.24, p = .044); the signs of the correlation between automatic associations and the smile FE score was in the expected direction but remained nonsignificant (β = -0.21, p = .078). HRV reactivity predicted self-reported exercise behavior (β = -0.28, p = .013) (the same pattern of results was achieved for the frown FE score). The HRV-related results illustrate the potential role of automatic negative affective reactions to the thought of exercise as a restraining force in exercise motivation. For better empirical distinction between the two ART type‑1 process components, automatic associations and the affective valuation should perhaps be measured separately in the future. The results support the notion that automatic and affective processes should be regarded as essential aspects of the motivation to exercise.}, language = {en} } @article{DeutschmannRoggenbuckSchieracketal.2020, author = {Deutschmann, Claudia and Roggenbuck, Dirk and Schierack, Peter and R{\"o}diger, Stefan}, title = {Autoantibody testing by enzyme-linked immunosorbent assay-a case in which the solid phase decides on success and failure}, series = {Heliyon}, volume = {6}, journal = {Heliyon}, number = {1}, publisher = {Elsevier}, address = {London [u.a.]}, issn = {2405-8440}, doi = {10.1016/j.heliyon.2020.e03270}, pages = {6}, year = {2020}, abstract = {Background: The enzyme-linked immunosorbent assay (ELISA) is an indispensable tool for clinical diagnostics to identify or differentiate diseases such as autoimmune illnesses, but also to monitor their progression or control the efficacy of drugs. One use case of ELISA is to differentiate between different states (e.g. healthy vs. diseased). Another goal is to quantitatively assess the biomarker in question, like autoantibodies. Thus, the ELISA technology is used for the discovery and verification of new autoantibodies, too. Of key interest, however, is the development of immunoassays for the sensitive and specific detection of such biomarkers at early disease stages. Therefore, users have to deal with many parameters, such as buffer systems or antigen-autoantibody interactions, to successfully establish an ELISA. Often, fine-tuning like testing of several blocking substances is performed to yield high signal-to-noise ratios.
Methods: We developed an ELISA to detect IgA and IgG autoantibodies against chitinase-3-like protein 1 (CHI3L1), a newly identified autoantigen in inflammatory bowel disease (IBD), in the serum of control and disease groups (n = 23, respectively). Microwell plates with different surface modifications (PolySorp and MaxiSorp coating) were tested to detect reproducibility problems.
Results: We found a significant impact of the surface properties of the microwell plates. IgA antibody reactivity was significantly lower, since it was in the range of background noise, when measured on MaxiSorp coated plates (p < 0.0001). The IgG antibody reactivity did not differ on the diverse plates, but the plate surface had a significant influence on the test result (p = 0.0005).
Conclusion: With this report, we want to draw readers' attention to the properties of solid phases and their effects on the detection of autoantibodies by ELISA. We want to sensitize the reader to the fact that the choice of the wrong plate can lead to a false negative test result, which in turn has serious consequences for the discovery of autoantibodies.}, language = {en} } @article{PuppeLeueSommeretal.2022, author = {Puppe, Daniel and Leue, Martin and Sommer, Michael and Schaller, J{\"o}rg and Kaczorek, Danuta}, title = {Auto-fluorescence in phytoliths}, series = {Frontiers in Environmental Science}, volume = {10}, journal = {Frontiers in Environmental Science}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {2296-665X}, doi = {10.3389/fenvs.2022.915947}, pages = {14}, year = {2022}, abstract = {The detection of auto-fluorescence in phytogenic, hydrated amorphous silica depositions (phytoliths) has been found to be a promising approach to verify if phytoliths were burnt or not, especially in archaeological contexts. However, it is unknown so far at what temperature and how auto-fluorescence is induced in phytoliths. We used fluorescence microscopy, scanning electron microscope-energy dispersive X-ray spectroscopy (SEM-EDX), and Fourier transform infrared spectroscopy to analyze auto-fluorescence in modern phytoliths extracted from plant samples or in intact leaves of winter wheat. Leaves and extracted phytoliths were heated at different temperatures up to 600 degrees C. The aims of our experiments were i) to find out what temperature is needed to induce auto-fluorescence in phytoliths, ii) to detect temperature-dependent changes in the molecular structure of phytoliths related to auto-fluorescence, and iii) to derive a mechanistic understanding of auto-fluorescence in phytoliths. We found organic compounds associated with phytoliths to cause auto-fluorescence in phytoliths treated at temperatures below approx. 400 degrees C. In phytoliths treated at higher temperatures, i.e., 450 and 600 degrees C, phytolith auto-fluorescence was mainly caused by molecular changes of phytolith silica. Based on our results we propose that auto-fluorescence in phytoliths is caused by clusterization-triggered emissions, which are caused by overlapping electron clouds forming non-conventional chromophores. In phytoliths heated at temperatures above about 400 degrees C dihydroxylation and the formation of siloxanes result in oxygen clusters that serve as non-conventional chromophores in fluorescence events. Furthermore, SEM-EDX analyses revealed that extractable phytoliths were dominated by lumen phytoliths (62\%) compared to cell wall phytoliths (38\%). Our findings might be not only relevant in archaeological phytolith-based examinations, but also for studies on the temperature-dependent release of silicon from phytoliths and the potential of long-term carbon sequestration in phytoliths.}, language = {en} } @article{KluthLemireTremblayJameyetal.2023, author = {Kluth, Alicia and Lemire-Tremblay, Marilyne and Jamey, Kevin and Dalla Bella, Simone and Falk, Simone}, title = {Auswirkungen eines nonverbalen Rhythmustrainings auf die Sprechmotorik und Sprechfl{\"u}ssigkeit bei stotternden Kindern}, series = {Spektrum Patholinguistik 16}, journal = {Spektrum Patholinguistik 16}, number = {16}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-559-0}, issn = {1866-9433}, doi = {10.25932/publishup-61349}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-613497}, pages = {135 -- 149}, year = {2023}, abstract = {Die alleinige Ursache von Stottern ist weiterhin nicht abschließend gekl{\"a}rt. Auf neurofunktioneller und -morphologischer Ebene zeigen stotternde Personen Abweichungen in den Arealen, welche nicht nur f{\"u}r die Initiierung von sprechmotorischen Programmen, sondern auch f{\"u}r die Produktion und Perzeption von musikalischen Rhythmen zust{\"a}ndig sind. Unterschiede zu fl{\"u}ssigsprechenden Personen treten jedoch nicht nur im verbalen, sondern auch im nonverbal sensomotorischen Bereich auf, was auf eine St{\"o}rung dom{\"a}nen{\"u}bergreifender auditiv-motorischer Koordinationsprozesse hindeutet. Ausgehend davon wurde in einigen Studien postuliert, dass dem St{\"o}rungsbild Stottern ein dom{\"a}nen{\"u}bergreifendes Rhythmusdefizit zugrunde liegt. Im Gegensatz zu anderen St{\"o}rungsbildern, welche auch Probleme in rhythmischen Aufgaben aufweisen, gibt es bis dato jedoch keine Intervention, welche dieses Defizit angeht. Es soll untersucht werden, ob ein nonverbales rhythmisches Training bei stotternden Kindern im Alter von zehn bis zw{\"o}lf Jahren eine Verbesserung der sensomotorischen Synchronisationsf{\"a}higkeiten zur Folge hat. Zudem soll erforscht werden, ob es Transfereffekte auf die Sprechmotorik und Sprechfl{\"u}ssigkeit gibt. F{\"u}r die vorliegende einfach verblindete Pilotstudie sollen 20 Kinder rekrutiert werden. Zehn davon werden randomisiert der Experimentalgruppe zugewiesen, welche das rhythmische Spiel Rhythm Workers {\"u}ber drei Wochen daheim auf einem Tablet spielen sollen. Die Kontrollgruppe spielt ein nicht-rhythmisches Bubble-Shooter-Spiel namens Frozen Bubble. Diese Studie k{\"o}nnte somit den Weg ebnen f{\"u}r technologieunterst{\"u}tzte Rehabilitationsmaßnahmen, welche darauf abzielen die rhythmisch-motorischen Defizite bei Stottern zu lindern.}, language = {de} } @article{AscheBrechenmacher2022, author = {Asche, Matthias and Brechenmacher, Thomas}, title = {Auswahlbibliographie zur berlin-brandenburgischen Immigrationsgeschichte}, series = {Hier geblieben? Brandenburg als Einwanderungsland vom Mittelalter bis heute}, journal = {Hier geblieben? Brandenburg als Einwanderungsland vom Mittelalter bis heute}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-506-4}, doi = {10.25932/publishup-54689}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-546899}, pages = {251 -- 262}, year = {2022}, language = {de} } @article{PatonVogelKlugeetal.2021, author = {Paton, Eva and Vogel, Johannes Joscha and Kluge, Bj{\"o}rn and Nehls, Thomas}, title = {Ausmaß, Trend und Extrema von D{\"u}rren im urbanen Raum}, series = {Hydrologie und Wasserbewirtschaftung}, volume = {65}, journal = {Hydrologie und Wasserbewirtschaftung}, number = {1}, publisher = {Bundesanstalt f{\"u}r Gew{\"a}sserkunde}, address = {Koblenz}, issn = {1439-1783}, doi = {10.5675/HyWa_2021.1_1}, pages = {5 -- 16}, year = {2021}, abstract = {Summers are currently perceived to be getting longer, hotter and more extreme - and this impression is reinforced in urban areas by the occurrence of heat island effects in densely built-up areas. To assess the real extent of increasing drought occurrences in German cities, a DWD data set of 31 urban climate stations for the period 1950 to 2019 was analysed using the standardised precipitation index (SPI) with regard to meteorological drought lengths, drought extrema, heat waves and compound events in the form of simultaneously occurring heat waves and drought months. The analysis shows a large degree of heterogeneity within Germany: a severe drought occurred in most cities in 2018, while the year 2018 was among the three years with the longest droughts (since 1950) for only one third of the cities. Some southern and central German cities show a statistically significant increase in drought months per decade since 1950, other cities, mostly in the north and northwest, only show an increase in the past two decades or even no trend at all. The compound analysis of simultaneously occurring heat and drought months shows a strong increase at most stations in the last two decades, whereby the two components are responsible with a very different proportion regionally for the increase in compound events.}, language = {de} } @article{Martin2022, author = {Martin, Angela}, title = {Ausgebeutet, diskriminiert, aber dringend gebraucht}, series = {Hier geblieben? Brandenburg als Einwanderungsland vom Mittelalter bis heute}, journal = {Hier geblieben? Brandenburg als Einwanderungsland vom Mittelalter bis heute}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-506-4}, doi = {10.25932/publishup-54685}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-546850}, pages = {193 -- 208}, year = {2022}, language = {de} } @article{Dieter2022, author = {Dieter, Heribert}, title = {AUKUS und die strukturellen Ver{\"a}nderungen der sicherheitspolitischen Lage im indo-pazifischen Raum}, series = {SIRIUS - Zeitschrift f{\"u}r Strategische Analysen}, volume = {6}, journal = {SIRIUS - Zeitschrift f{\"u}r Strategische Analysen}, number = {2}, publisher = {De Gruyter}, address = {Berlin}, issn = {2510-263X}, doi = {10.1515/sirius-2022-2007}, pages = {210 -- 217}, year = {2022}, language = {de} }