@phdthesis{Wenk2020, author = {Wenk, Sebastian}, title = {Engineering formatotrophic growth in Escherichia coli}, school = {Universit{\"a}t Potsdam}, pages = {V, 107}, year = {2020}, abstract = {To meet the demands of a growing world population while reducing carbon dioxide (CO2) emissions, it is necessary to capture CO2 and convert it into value-added compounds. In recent years, metabolic engineering of microbes has gained strong momentum as a strategy for the production of valuable chemicals. As common microbial feedstocks like glucose directly compete with human consumption, the one carbon (C1) compound formate was suggested as an alternative feedstock. Formate can be easily produced by various means including electrochemical reduction of CO2 and could serve as a feedstock for microbial production, hence presenting a novel entry point for CO2 to the biosphere and a storage option for excess electricity. Compared to the gaseous molecule CO2, formate is a highly soluble compound that can be easily handled and stored. It can serve as a carbon and energy source for natural formatotrophs, but these microbes are difficult to cultivate and engineer. In this work, I present the results of several projects that aim to establish efficient formatotrophic growth of E. coli - which cannot naturally grow on formate - via synthetic formate assimilation pathways. In the first study, I establish a workflow for growth-coupled metabolic engineering of E. coli. I demonstrate this approach by presenting an engineering scheme for the PFL-threonine cycle, a synthetic pathway for anaerobic formate assimilation in E. coli. The described methods are intended to create a standardized toolbox for engineers that aim to establish novel metabolic routes in E. coli and related organisms. The second chapter presents a study on the catalytic efficiency of C1-oxidizing enzymes in vivo. As formatotrophic growth requires generation of both energy and biomass from formate, the engineered E. coli strains need to be equipped with a highly efficient formate dehydrogenase, which provides reduction equivalents and ATP for formate assimilation. I engineered a strain that cannot generate reducing power and energy for cellular growth, when fed on acetate. Under this condition, the strain depends on the introduction of an enzymatic system for NADH regeneration, which could further produce ATP via oxidative phosphorylation. I show that the strain presents a valuable testing platform for C1-oxidizing enzymes by testing different NAD-dependent formate and methanol dehydrogenases in the energy auxotroph strain. Using this platform, several candidate enzymes with high in vivo activity, were identified and characterized as potential energy-generating systems for synthetic formatotrophic or methylotrophic growth in E. coli.   In the third chapter, I present the establishment of the serine threonine cycle (STC) - a synthetic formate assimilation pathway - in E. coli. In this pathway, formate is assimilated via formate tetrahydrofolate ligase (FtfL) from Methylobacterium extorquens (M. extorquens). The carbon from formate is attached to glycine to produce serine, which is converted into pyruvate entering central metabolism. Via the natural threonine synthesis and cleavage route, glycine is regenerated and acetyl-CoA is produced as the pathway product. I engineered several selection strains that depend on different STC modules for growth and determined key enzymes that enable high flux through threonine synthesis and cleavage. I could show that expression of an auxiliary formate dehydrogenase was required to achieve growth via threonine synthesis and cleavage on pyruvate. By overexpressing most of the pathway enzymes from the genome, and applying adaptive laboratory evolution, growth on glycine and formate was achieved, indicating the activity of the complete cycle. The fourth chapter shows the establishment of the reductive glycine pathway (rGP) - a short, linear formate assimilation route - in E. coli. As in the STC, formate is assimilated via M. extorquens FtfL. The C1 from formate is condensed with CO2 via the reverse reaction of the glycine cleavage system to produce glycine. Another carbon from formate is attached to glycine to form serine, which is assimilated into central metabolism via pyruvate. The engineered E. coli strain, expressing most of the pathway genes from the genome, can grow via the rGP with formate or methanol as a sole carbon and energy source.}, language = {en} } @phdthesis{Schimpf2020, author = {Schimpf, Stefan}, title = {Herkunft und Ablagerungsmilieu quart{\"a}rer Sedimente im Einzugsgebiet des Heihe, NW China}, school = {Universit{\"a}t Potsdam}, pages = {xi, 186}, year = {2020}, abstract = {Der zentralasiatische Naturraum, wie er sich uns heute pr{\"a}sentiert, ist das Ergebnis eines Zusammenwirkens vieler verschiedener Faktoren {\"u}ber Jahrmillionen hinweg. Im aktuellen Kontext des Klimawandels zeigt sich jedoch, wie stark sich Stofffl{\"u}sse auch kurzfristig {\"a}ndern und dabei das Gesicht der Landschaft verwandeln k{\"o}nnen. Die Gobi-W{\"u}ste in der Inneren Mongolei (China), als Teil der gleichnamigen Trockenregionen Nordwestchinas, ist aufgrund der Ausgestaltung ihrer landschaftspr{\"a}genden Elemente sowie ihrer Landschaftsdynamik, im Zusammenhang mit der Lage zum Tibet-Plateau, in den Fokus der klimageschichtlichen Grundlagenforschung ger{\"u}ckt. Als großes Langzeitarchiv unterschiedlichster fluvialer, lakustriner und {\"a}olischer Sedimente stellt sie eine bedeutende Lokalit{\"a}t zur Rekonstruktion von lokalen und regionalen Stofffl{\"u}ssen dar.. Andererseits ist die Gobi-W{\"u}ste zugleich auch eine bedeutende Quelle f{\"u}r den {\"u}berregionalen Staubtransport, da sie aufgrund der klimatischen Bedingungen insbesondere der Erosion durch Ausblasung preisgegeben wird. Vor diesem Hintergrund erfolgten zwischen 2011 und 2014, im Rahmen des BMBF-Verbundprogramms WTZ Zentralasien - Monsundynamik \& Geo{\"o}kosysteme (F{\"o}rderkennzeichen 03G0814), mehrere deutsch-chinesische Expeditionen in das Ejina-Becken (Innere Mongolei) und das Qilian Shan-Vorland. Im Zuge dieser Expeditionen wurden f{\"u}r eine Bestimmung potenzieller Sedimentquellen erstmals zahlreiche Oberfl{\"a}chenproben aus dem gesamten Einzugsgebiet des Heihe (schwarzer Fluss) gesammelt. Zudem wurden mit zwei Bohrungen im inneren des Ejina-Beckens, erg{\"a}nzende Sedimentbohrkerne zum bestehenden Bohrkern D100 (siehe W{\"u}nnemann (2005)) abgeteuft, um weit reichende, erg{\"a}nzende Informationen zur Landschaftsgeschichte und zum {\"u}berregionalen Sedimenttransfer zu erhalten. Gegenstand und Ziel der vorliegenden Doktorarbeit ist die sedimentologisch-mineralogische Charakterisierung des Untersuchungsgebietes in Bezug auf potenzielle Sedimentquellen und Stofffl{\"u}sse des Ejina-Beckens sowie die Rekonstruktion der Ablagerungsgeschichte eines dort erbohrten, 19m langen Sedimentbohrkerns (GN100). Schwerpunkt ist hierbei die Kl{\"a}rung der Sedimentherkunft innerhalb des Bohrkerns sowie die Ausweisung von Herkunftssignalen und m{\"o}glichen Sedimentquellen bzw. Sedimenttransportpfaden. Die methodische Herangehensweise basiert auf einem Multi-Proxy-Ansatz zur Charakterisierung der klastischen Sedimentfazies anhand von Gel{\"a}ndebeobachtungen, lithologisch-granulometrischen und mineralogisch-geochemischen Analysen sowie statistischen Verfahren. F{\"u}r die mineralogischen Untersuchungen der Sedimente wurde eine neue, rasterelektronenmikroskopische Methode zur automatisierten Partikelanalyse genutzt und den traditionellen Methoden gegen{\"u}bergestellt. Die synoptische Betrachtung der granulometrischen, geochemischen und mineralogischen Befunde der Oberfl{\"a}chensedimente ergibt f{\"u}r das Untersuchungsgebiet ein logisches Kaskadenmodell mit immer wiederkehrenden Prozessbereichen und {\"a}hnlichen Prozesssignalen. Die umfangreichen granulometrischen Analysen deuten dabei auf abnehmende Korngr{\"o}ßen mit zunehmender Entfernung vom Qilian Shan hin und erm{\"o}glichen die Identifizierung von vier texturellen Signalen: den fluvialen Sanden, den D{\"u}nensanden, den Stillwassersedimenten und St{\"a}uben. Diese Ergebnisse k{\"o}nnen als Interpretationsgrundlage f{\"u}r die Korngr{\"o}ßenanalysen des Bohrkerns genutzt werden. Somit ist es m{\"o}glich, die Ablagerungsgeschichte der Bohrkernsedimente zu rekonstruieren und in Verbindung mit eigenen und literaturbasierten Datierungen in einen Gesamtkontext einzuh{\"a}ngen. F{\"u}r das Untersuchungsgebiet werden somit vier Ablagerungsphasen ausgewiesen, die bis in die Zeit des letzten glazialen Maximums (LGM) zur{\"u}ckreichen. W{\"a}hrend dieser Ablagerungsphasen kam es im Zuge unterschiedlicher Aktivit{\"a}ts- und Stabilit{\"a}tsphasen zu einer kontinuierlichen Progradation und {\"U}berpr{\"a}gung des Schwemmf{\"a}chers. Eine besonders aktive Phase kann zwischen 8 ka und 4 ka BP festgestellt werden, w{\"a}hrend der es aufgrund zunehmender fluvialer Aktivit{\"a}ten zu einer deutlich verst{\"a}rkten Schwemmf{\"a}cherdynamik gekommen zu sein scheint. In den Abschnitten davor und danach waren es vor allem {\"a}olische Prozesse, die zu einer {\"U}berpr{\"a}gung des Schwemmf{\"a}chers gef{\"u}hrt haben. Hinsichtlich der mineralogischen Herkunftssignale gibt es eine große Variabilit{\"a}t. Dies spiegelt die enorme Heterogenit{\"a}t der Geologie des Untersuchungsgebietes wider, wodurch die r{\"a}umlichen Signale nicht sehr stark ausgepr{\"a}gt sind. Dennoch, k{\"o}nnen f{\"u}r das Einzugsgebiet drei gr{\"o}ßere Bereiche deklariert werden, die als Herkunftsgebiet in Frage kommen. Das {\"o}stliche Qilian Shan Vorland zeichnet sich dabei durch deutlich h{\"o}here Chloritgehalte als prim{\"a}re Quelle f{\"u}r die Sedimente im Ejina-Becken aus. Sie unterscheiden sich insbesondere durch stark divergierende Chloritgehalte in der Tonmineral- und Gesamtmineralfraktion, was das {\"o}stliche Qilian Shan Vorland als prim{\"a}re Quelle f{\"u}r die Sedimente im Ejina-Becken auszeichnet. Dies steht in Zusammenhang mit den Gr{\"u}nschiefern, Ophioliten und Serpentiniten in diesem Bereich. Geochemisch deutet vor allem das Cr/Rb-Verh{\"a}ltnis eine große Variabilit{\"a}t innerhalb des Einzugsgebietes an. Auch hier ist es das {\"o}stliche Vorland, welches aufgrund seines hohen Anteils an mafischen Gesteinen reich an Chromiten und Spinellen ist und sich somit vom restlichen Untersuchungsgebiet abhebt. Die zeitliche aber auch die generelle Variabilit{\"a}t der Sedimentherkunft l{\"a}sst sich in den Bohrkernsedimenten nicht so deutlich nachzeichnen. Die mineralogisch-sedimentologischen Eigenschaften der erbohrten klastischen Sedimente zeugen zwar von zwischenzeitlichen {\"A}nderungen bei der Sedimentherkunft, diese sind jedoch nicht so deutlich ausgepr{\"a}gt, wie es die Quellsignale in den Oberfl{\"a}chensedimenten vermuten lassen. Ein Grund daf{\"u}r scheint die starke Vermischung unterschiedlichster Sedimente w{\"a}hrend des Transportes zu sein. Die Kombination der Korngr{\"o}ßenergebnisse mit den Befunden der Gesamt- und Schwermineralogie deuten darauf hin, dass es zwischenzeitlich eine Phase mit {\"u}berwiegend {\"a}olischen Prozessen gegeben hat, die mit einem Sedimenteintrag aus dem westlichen Bei Shan in Verbindung stehen. Neben der Zunahme ultrastabiler Schwerminerale wie Zirkon und Granat und der Abnahme opaker Schwerminerale, weisen vor allem die heutigen Verh{\"a}ltnisse darauf hin. Der Vergleich der traditionellen Schwermineralanalyse mit der Computer-Controlled-Scanning-Electron-Microscopy (kurz: CCSEM), die eine automatisierte Partikelauswertung der Proben erm{\"o}glicht, zeigt den deutlichen Vorteil der modernen Analysemethode. Neben einem zeitlichen Vorteil, den man durch die automatisierte Abarbeitung der vorbereiteten Proben erlangen kann, steht vor allem die deutlich gr{\"o}ßere statistische Signifikanz des Ergebnisses im Vordergrund. Zudem k{\"o}nnen mit dieser Methode auch chemische Variet{\"a}ten einiger Schwerminerale bestimmt werden, die eine noch feinere Klassifizierung und sicherere Aussagen zu einer m{\"o}glichen Sedimentherkunft erm{\"o}glichen. Damit ergeben sich außerdem verbesserte Aussagen zu Zusammensetzungen und Entstehungsprozessen der abgelagerten Sedimente. Die Studie verdeutlicht, dass die Sedimentherkunft innerhalb des Untersuchungsgebietes sowie die ablaufenden Prozesse zum Teil stark von lokalen Gegebenheiten abh{\"a}ngen. Die Heterogenit{\"a}t der Geologie und die Gr{\"o}ße des Einzugsgebietes sowie die daraus resultierende Komplexit{\"a}t der Sedimentgenese, machen exakte Zuordnungen zu klar definierten Sedimentquellen sehr schwer. Dennoch zeigen die Ergebnisse, dass die Sedimentzufuhr in das Ejina-Becken in erster Linie durch fluviale klastische Sedimente des Heihe aus dem Qilian Shan erfolgt sein muss. Die Untersuchungsergebnisse zeigen jedoch ebenso die Notwendigkeit einer erg{\"a}nzenden Bearbeitung angrenzender Untersuchungsgebiete, wie beispielsweise den Gobi-Altai im Norden oder den Beishan im Westen, sowie die Verdichtung der Oberfl{\"a}chenbeprobung zur feineren Aufl{\"o}sung von lokalen Sedimentquellen.}, language = {de} } @article{TofeldeBufeTurowski2022, author = {Tofelde, Stefanie and Bufe, Aaron and Turowski, Jens M.}, title = {Hillslope Sediment Supply Limits Alluvial Valley Width}, series = {AGU Advances}, journal = {AGU Advances}, publisher = {American Geophysical Union (AGU); Wiley}, address = {Hoboken, New Jersey, USA}, issn = {2576-604X}, doi = {10.1029/2021AV000641}, pages = {20}, year = {2022}, abstract = {River-valley morphology preserves information on tectonic and climatic conditions that shape landscapes. Observations suggest that river discharge and valley-wall lithology are the main controls on valley width. Yet, current models based on these observations fail to explain the full range of cross-sectional valley shapes in nature, suggesting hitherto unquantified controls on valley width. In particular, current models cannot explain the existence of paired terrace sequences that form under cyclic climate forcing. Paired river terraces are staircases of abandoned floodplains on both valley sides, and hence preserve past valley widths. Their formation requires alternating phases of predominantly river incision and predominantly lateral planation, plus progressive valley narrowing. While cyclic Quaternary climate changes can explain shifts between incision and lateral erosion, the driving mechanism of valley narrowing is unknown. Here, we extract valley geometries from climatically formed, alluvial river-terrace sequences and show that across our dataset, the total cumulative terrace height (here: total valley height) explains 90\%-99\% of the variance in valley width at the terrace sites. This finding suggests that valley height, or a parameter that scales linearly with valley height, controls valley width in addition to river discharge and lithology. To explain this valley-width-height relationship, we reformulate existing valley-width models and suggest that, when adjusting to new boundary conditions, alluvial valleys evolve to a width at which sediment removal from valley walls matches lateral sediment supply from hillslope erosion. Such a hillslope-channel coupling is not captured in current valley-evolution models. Our model can explain the existence of paired terrace sequences under cyclic climate forcing and relates valley width to measurable field parameters. Therefore, it facilitates the reconstruction of past climatic and tectonic conditions from valley topography.}, language = {en} } @article{MiklashevskyFischerLindemann2022, author = {Miklashevsky, Alex and Fischer, Martin H. and Lindemann, Oliver}, title = {Spatial-numerical associations without a motor response? Grip force says 'Yes'}, series = {Acta Psychologica}, volume = {231}, journal = {Acta Psychologica}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-6297}, doi = {10.1016/j.actpsy.2022.103791}, pages = {1 -- 17}, year = {2022}, abstract = {In numerical processing, the functional role of Spatial-Numerical Associations (SNAs, such as the association of smaller numbers with left space and larger numbers with right space, the Mental Number Line hypothesis) is debated. Most studies demonstrate SNAs with lateralized responses, and there is little evidence that SNAs appear when no response is required. We recorded passive holding grip forces in no-go trials during number processing. In Experiment 1, participants performed a surface numerical decision task ("Is it a number or a letter?"). In Experiment 2, we used a deeper semantic task ("Is this number larger or smaller than five?"). Despite instruction to keep their grip force constant, participants' spontaneous grip force changed in both experiments: Smaller numbers led to larger force increase in the left than in the right hand in the numerical decision task (500-700 ms after stimulus onset). In the semantic task, smaller numbers again led to larger force increase in the left hand, and larger numbers increased the right-hand holding force. This effect appeared earlier (180 ms) and lasted longer (until 580 ms after stimulus onset). This is the first demonstration of SNAs with passive holding force. Our result suggests that (1) explicit motor response is not a prerequisite for SNAs to appear, and (2) the timing and strength of SNAs are task-dependent. (216 words).}, language = {en} } @techreport{KienbaumFischerPassmann2023, type = {Working Paper}, author = {Kienbaum, Janna and Fischer, Patryk and Passmann, Sven}, title = {Forschungsdatenmanagement bei personenbezogenen Daten}, doi = {10.5281/zenodo.7428524}, pages = {18}, year = {2023}, abstract = {Das Dokument "Forschungsdatenmanagement bei personenbezogenen Daten - eine Handreichung" versammelt zentrale Inhalte, Verweise und Vorgehensweisen f{\"u}r Forscher*innen, die in einer Studie personenbezogene Daten erheben und diese verarbeiten, archivieren oder ver{\"o}ffentlichen wollen. Die Handreichung verweist an den entsprechenden Abschnitten auf weiterf{\"u}hrende Materialien wie insbesondere die Handreichung „Datenschutz" des Rats f{\"u}r die Sozial-, Verhaltens-, Bildungs- und Wirtschaftswissenschaften (RatSWD).}, language = {de} } @techreport{KienbaumWuerflFavella2022, type = {Working Paper}, author = {Kienbaum, Janna and W{\"u}rfl, Katja and Favella, Gianpiero}, title = {Leitfaden f{\"u}r einen qualitativen Methoden- und Feldbericht}, doi = {10.5281/zenodo.7247993}, pages = {12}, year = {2022}, abstract = {Der Leitfaden f{\"u}r einen qualitativen Methoden- und Feldbericht ist im Rahmen des DFG-Verbundprojektes "FDNext" (2020-2023) entstanden und dient als Orientierungshilfe f{\"u}r die Dokumentation erhobener Daten mit dem Schwerpunkt auf die qualitative Bildungsforschung.}, language = {de} } @article{LindnerMoellerHildebrandtetal.2022, author = {Lindner, Nadja and Moeller, Korbinian and Hildebrandt, Frauke and Hasselhorn, Marcus and Lonnemann, Jan}, title = {Children's use of egocentric reference frames in spatial language is related to their numerical magnitude understanding}, series = {Frontiers in Psychology}, journal = {Frontiers in Psychology}, publisher = {Frontiers}, address = {Lausanne, Schweiz}, issn = {1664-1078}, doi = {10.3389/fpsyg.2022.943191}, pages = {1 -- 13}, year = {2022}, abstract = {Numerical magnitude information is assumed to be spatially represented in the form of a mental number line defined with respect to a body-centred, egocentric frame of reference. In this context, spatial language skills such as mastery of verbal descriptions of spatial position (e.g., in front of, behind, to the right/left) have been proposed to be relevant for grasping spatial relations between numerical magnitudes on the mental number line. We examined 4- to 5-year-old's spatial language skills in tasks that allow responses in egocentric and allocentric frames of reference, as well as their relative understanding of numerical magnitude (assessed by a number word comparison task). In addition, we evaluated influences of children's absolute understanding of numerical magnitude assessed by their number word comprehension (montring different numbers using their fingers) and of their knowledge on numerical sequences (determining predecessors and successors as well as identifying missing dice patterns of a series). Results indicated that when considering responses that corresponded to the egocentric perspective, children's spatial language was associated significantly with their relative numerical magnitude understanding, even after controlling for covariates, such as children's SES, mental rotation skills, and also absolute magnitude understanding or knowledge on numerical sequences. This suggests that the use of egocentric reference frames in spatial language may facilitate spatial representation of numbers along a mental number line and thus seem important for preschoolers' relative understanding of numerical magnitude.}, language = {en} } @article{HeckerSteckhanEybenetal.2022, author = {Hecker, Pascal and Steckhan, Nico and Eyben, Florian and Schuller, Bj{\"o}rn Wolfgang and Arnrich, Bert}, title = {Voice Analysis for Neurological Disorder Recognition - A Systematic Review and Perspective on Emerging Trends}, series = {Frontiers in Digital Health}, journal = {Frontiers in Digital Health}, publisher = {Frontiers Media SA}, address = {Lausanne, Schweiz}, issn = {2673-253X}, doi = {10.3389/fdgth.2022.842301}, pages = {16}, year = {2022}, abstract = {Quantifying neurological disorders from voice is a rapidly growing field of research and holds promise for unobtrusive and large-scale disorder monitoring. The data recording setup and data analysis pipelines are both crucial aspects to effectively obtain relevant information from participants. Therefore, we performed a systematic review to provide a high-level overview of practices across various neurological disorders and highlight emerging trends. PRISMA-based literature searches were conducted through PubMed, Web of Science, and IEEE Xplore to identify publications in which original (i.e., newly recorded) datasets were collected. Disorders of interest were psychiatric as well as neurodegenerative disorders, such as bipolar disorder, depression, and stress, as well as amyotrophic lateral sclerosis amyotrophic lateral sclerosis, Alzheimer's, and Parkinson's disease, and speech impairments (aphasia, dysarthria, and dysphonia). Of the 43 retrieved studies, Parkinson's disease is represented most prominently with 19 discovered datasets. Free speech and read speech tasks are most commonly used across disorders. Besides popular feature extraction toolkits, many studies utilise custom-built feature sets. Correlations of acoustic features with psychiatric and neurodegenerative disorders are presented. In terms of analysis, statistical analysis for significance of individual features is commonly used, as well as predictive modeling approaches, especially with support vector machines and a small number of artificial neural networks. An emerging trend and recommendation for future studies is to collect data in everyday life to facilitate longitudinal data collection and to capture the behavior of participants more naturally. Another emerging trend is to record additional modalities to voice, which can potentially increase analytical performance.}, language = {en} } @article{OgunkolaGuiraudieCaprazFeronetal.2023, author = {Ogunkola, Moses Olalekan and Guiraudie-Capraz, Gaelle and F{\´e}ron, Fran{\c{c}}ois and Leimk{\"u}hler, Silke}, title = {The Human Mercaptopyruvate Sulfurtransferase TUM1 Is Involved in Moco Biosynthesis, Cytosolic tRNA Thiolation and Cellular Bioenergetics in Human Embryonic Kidney Cells}, series = {Biomolecules}, volume = {13}, journal = {Biomolecules}, edition = {1}, publisher = {MDPI}, address = {Basel, Schweiz}, issn = {2218-273X}, doi = {10.3390/biom13010144}, pages = {1 -- 23}, year = {2023}, abstract = {Sulfur is an important element that is incorporated into many biomolecules in humans. The incorporation and transfer of sulfur into biomolecules is, however, facilitated by a series of different sulfurtransferases. Among these sulfurtransferases is the human mercaptopyruvate sulfurtransferase (MPST) also designated as tRNA thiouridine modification protein (TUM1). The role of the human TUM1 protein has been suggested in a wide range of physiological processes in the cell among which are but not limited to involvement in Molybdenum cofactor (Moco) biosynthesis, cytosolic tRNA thiolation and generation of H2S as signaling molecule both in mitochondria and the cytosol. Previous interaction studies showed that TUM1 interacts with the L-cysteine desulfurase NFS1 and the Molybdenum cofactor biosynthesis protein 3 (MOCS3). Here, we show the roles of TUM1 in human cells using CRISPR/Cas9 genetically modified Human Embryonic Kidney cells. Here, we show that TUM1 is involved in the sulfur transfer for Molybdenum cofactor synthesis and tRNA thiomodification by spectrophotometric measurement of the activity of sulfite oxidase and liquid chromatography quantification of the level of sulfur-modified tRNA. Further, we show that TUM1 has a role in hydrogen sulfide production and cellular bioenergetics.}, language = {en} } @article{GoeldelKamrathMindenetal.2022, author = {G{\"o}ldel, Julia M. and Kamrath, Clemens and Minden, Kirsten and Wiegand, Susanna and Lanzinger, Stefanie and Sengler, Claudia and Weihrauch-Bl{\"u}her, Susann and Holl, Reinhard W. and Tittel, Sascha Ren{\´e} and Warschburger, Petra}, title = {Access to Healthcare for Children and Adolescents with a Chronic Health Condition during the COVID-19 Pandemic: First Results from the KICK-COVID Study in Germany}, series = {Children}, volume = {10}, journal = {Children}, edition = {1}, publisher = {MDPI}, address = {Basel, Schweiz}, issn = {2227-9067}, doi = {10.3390/children10010010}, pages = {1 -- 11}, year = {2022}, abstract = {This study examines the access to healthcare for children and adolescents with three common chronic diseases (type-1 diabetes (T1D), obesity, or juvenile idiopathic arthritis (JIA)) within the 4th (Delta), 5th (Omicron), and beginning of the 6th (Omicron) wave (June 2021 until July 2022) of the COVID-19 pandemic in Germany in a cross-sectional study using three national patient registries. A paper-and-pencil questionnaire was given to parents of pediatric patients (<21 years) during the routine check-ups. The questionnaire contains self-constructed items assessing the frequency of healthcare appointments and cancellations, remote healthcare, and satisfaction with healthcare. In total, 905 parents participated in the T1D-sample, 175 in the obesity-sample, and 786 in the JIA-sample. In general, satisfaction with healthcare (scale: 0-10; 10 reflecting the highest satisfaction) was quite high (median values: T1D 10, JIA 10, obesity 8.5). The proportion of children and adolescents with canceled appointments was relatively small (T1D 14.1\%, JIA 11.1\%, obesity 20\%), with a median of 1 missed appointment, respectively. Only a few parents (T1D 8.6\%; obesity 13.1\%; JIA 5\%) reported obstacles regarding health services during the pandemic. To conclude, it seems that access to healthcare was largely preserved for children and adolescents with chronic health conditions during the COVID-19 pandemic in Germany.}, language = {en} }