@misc{BornhorstKippHaaseetal.2018,
  author    = {Bornhorst, Julia and Kipp, Anna Patricia and Haase, Hajo and Meyer, Soeren and Schwerdtle, Tanja},
  title     = {The crux of inept biomarkers for risks and benefits of trace elements},
  series = {Trends in Analytical Chemistry},
  volume    = {104},
  journal   = {Trends in Analytical Chemistry},
  publisher = {Elsevier},
  address   = {Oxford},
  issn      = {0165-9936},
  doi       = {10.1016/j.trac.2017.11.007},
  pages     = {183 -- 190},
  year      = {2018},
  abstract  = {Nowadays, the role of trace elements (TE) is of growing interest because dyshomeostasis of selenium (Se), manganese (Mn), zinc (Zn), and copper (Cu) is supposed to be a risk factor for several diseases. Thereby, research focuses on identifying new biomarkers for the TE status to allow for a more reliable description of the individual TE and health status. This review mirrors a lack of well-defined, sensitive, and selective biomarkers and summarizes technical limitations to measure them. Thus, the capacity to assess the relationship between dietary TE intake, homeostasis, and health is restricted, which would otherwise provide the basis to define adequate intake levels of single TE in both healthy and diseased humans. Besides that, our knowledge is even more limited with respect to the real life situation of combined TE intake and putative interactions between single TE.},
  language  = {en}
}
@article{FischerSaalfrank2021,
  author    = {Fischer, Eric Wolfgang and Saalfrank, Peter},
  title     = {A thermofield-based multilayer multiconfigurational time-dependent Hartree approach to non-adiabatic quantum dynamics at finite temperature},
  series = {The journal of chemical physics : bridges a gap between journals of physics and journals of chemistry},
  volume    = {155},
  journal   = {The journal of chemical physics : bridges a gap between journals of physics and journals of chemistry},
  number    = {13},
  publisher = {American Institute of Physics},
  address   = {Melville},
  issn      = {0021-9606},
  doi       = {10.1063/5.0064013},
  pages     = {15},
  year      = {2021},
  abstract  = {We introduce a thermofield-based formulation of the multilayer multiconfigurational time-dependent Hartree (MCTDH) method to study finite temperature effects on non-adiabatic quantum dynamics from a non-stochastic, wave function perspective. Our approach is based on the formal equivalence of bosonic many-body theory at zero temperature with a doubled number of degrees of freedom and the thermal quasi-particle representation of bosonic thermofield dynamics (TFD). This equivalence allows for a transfer of bosonic many-body MCTDH as introduced by Wang and Thoss to the finite temperature framework of thermal quasi-particle TFD. As an application, we study temperature effects on the ultrafast internal conversion dynamics in pyrazine. We show that finite temperature effects can be efficiently accounted for in the construction of multilayer expansions of thermofield states in the framework presented herein. Furthermore, we find our results to agree well with existing studies on the pyrazine model based on the pMCTDH method.},
  language  = {en}
}
@inproceedings{WangBreternitzSchorr2021,
  author    = {Wang, Zhenyu and Breternitz, Joachim and Schorr, Susan},
  title     = {Cation disorder in zinc-group IV- nitride and oxide nitride semiconductor materials revealed through neutron diffraction},
  series = {Acta crystallographica / International Union of Crystallography. Section A, Foundations and advances},
  volume    = {77},
  booktitle = {Acta crystallographica / International Union of Crystallography. Section A, Foundations and advances},
  number    = {Suppl.},
  publisher = {Blackwell},
  address   = {Oxford [u.a.]},
  issn      = {2053-2733},
  doi       = {10.1107/S0108767321086256},
  pages     = {C1077 -- C1077},
  year      = {2021},
  language  = {en}
}
@article{DambacherKlieglHofmannetal.2006,
  author    = {Dambacher, Michael and Kliegl, Reinhold and Hofmann, Markus and Jacobs, Arthur M.},
  title     = {Frequency and predictability effects on event-related potentials during reading},
  issn      = {0006-8993},
  doi       = {10.1016/j.brainres.2006.02.010},
  year      = {2006},
  abstract  = {Effects of frequency, predictability, and position of words on event-related potentials were assessed during word-by-word sentence reading in 48 subjects in an early and in a late time window corresponding to P200 and N400. Repeated measures multiple regression analyses revealed a P200 effect in the high-frequency range also the P200 was larger on words at the beginning and end of sentences than on words in the middle of sentences (i.e., a quadratic effect of word position). Predictability strongly affected the N400 component; the effect was stronger for low than for high- frequency words. The P200 frequency effect indicates that high-frequency words are lexically accessed very fast, independent of context information. Effects on the N400 suggest that predictability strongly moderates the late access especially of low-frequency words. Thus, contextual facilitation on the N400 appears to reflect both lexical and post- lexical stages of word recognition, questioning a strict classification into lexical and post-lexical processes.},
  language  = {en}
}
@article{GoettlichGraulichHuweretal.2022,
  author    = {G{\"o}ttlich, Richard and Graulich, Nicole and Huwer, Johannes and Banerji, Amitabh},
  title     = {Analog und digital},
  series = {Chemie konkret : CHEMKON ; Forum f{\"u}r Unterricht und Didaktik},
  volume    = {29},
  journal   = {Chemie konkret : CHEMKON ; Forum f{\"u}r Unterricht und Didaktik},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {0944-5846},
  doi       = {10.1002/ckon.202200046},
  pages     = {182 -- 182},
  year      = {2022},
  language  = {de}
}
@article{PiekarczykHeitmannWeissetal.2020,
  author    = {Piekarczyk, Andreas and Heitmann, Ulrike and Weiß, Karl-Anders and K{\"o}hl, Michael and Bald, Ilko},
  title     = {Development of a simple setup for temperature dependent mass spectrometric measurements for the investigation of outgassing effects in polymeric materials for solar application},
  series = {Polymer testing},
  volume    = {81},
  journal   = {Polymer testing},
  publisher = {Elsevier},
  address   = {Oxford},
  issn      = {0142-9418},
  doi       = {10.1016/j.polymertesting.2019.106164},
  pages     = {8},
  year      = {2020},
  abstract  = {A simple experimental setup for temperature dependent mass spectrometric measurements has been constructed. It consists of a heated sample chamber and a mass spectrometer and allows for measurements under inert gas and ambient air. Based on initial measurements on two extruded polystyrene (XPS) samples a methodology for the data analysis has been developed. With this methodology the outgassing temperature of volatile compounds, which were used as blowing agents, has been identified. Furthermore, the composition of the blowing agents has been analyzed by temperature dependent mass spectra. The results indicate the use of ambient air in one material and a mixture of the banned blowing agents R142b and R22, both hydrochlorofluorocarbons (HCFC), in the other material. The here described methodology provides an easy to use approach to identify such compounds, for example as part of environmental or quality control.},
  language  = {en}
}
@article{LiebigSarhanSchmittetal.2020,
  author    = {Liebig, Ferenc and Sarhan, Radwan Mohamed and Schmitt, Clemens Nikolaus Zeno and Th{\"u}nemann, Andreas F. and Prietzel, Claudia Christina and Bargheer, Matias and Koetz, Joachim},
  title     = {Gold nanotriangles with crumble topping and their influence on catalysis and surface-enhanced raman spectroscopy},
  series = {ChemPlusChem},
  volume    = {85},
  journal   = {ChemPlusChem},
  number    = {3},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {2192-6506},
  doi       = {10.1002/cplu.201900745},
  pages     = {519 -- 526},
  year      = {2020},
  abstract  = {By adding hyaluronic acid (HA) to dioctyl sodium sulfosuccinate (AOT)-stabilized gold nanotriangles (AuNTs) with an average thickness of 7.5 +/- 1 nm and an edge length of about 175 +/- 17 nm, the AOT bilayer is replaced by a polymeric HA-layer leading to biocompatible nanoplatelets. The subsequent reduction process of tetrachloroauric acid in the HA-shell surrounding the AuNTs leads to the formation of spherical gold nanoparticles on the platelet surface. With increasing tetrachloroauric acid concentration, the decoration with gold nanoparticles can be tuned. SAXS measurements reveal an increase of the platelet thickness up to around 14.5 nm, twice the initial value of bare AuNTs. HRTEM micrographs show welding phenomena between densely packed particles on the platelet surface, leading to a crumble formation while preserving the original crystal structure. Crumbles crystallized on top of the platelets enhance the Raman signal by a factor of around 20, and intensify the plasmon-driven dimerization of 4-nitrothiophenol (4-NTP) to 4,4 '-dimercaptoazobenzene in a yield of up to 50 \%. The resulting crumbled nanotriangles, with a biopolymer shell and the absorption maximum in the second window for in vivo imaging, are promising candidates for biomedical sensing.},
  language  = {en}
}
@article{DengWangXuaetal.2020,
  author    = {Deng, Zijun and Wang, Weiwei and Xua, Xun and Gould, Oliver E. C. and Kratz, Karl and Ma, Nan and Lendlein, Andreas},
  title     = {Polymeric sheet actuators with programmable bioinstructivity},
  series = {PNAS},
  volume    = {117},
  journal   = {PNAS},
  number    = {4},
  publisher = {National Academy of Sciences},
  address   = {Washington, DC},
  issn      = {1091-6490},
  doi       = {10.1073/pnas.1910668117},
  pages     = {1895 -- 1901},
  year      = {2020},
  abstract  = {Stem cells are capable of sensing and processing environmental inputs, converting this information to output a specific cell lineage through signaling cascades. Despite the combinatorial nature of mechanical, thermal, and biochemical signals, these stimuli have typically been decoupled and applied independently, requiring continuous regulation by controlling units. We employ a programmable polymer actuator sheet to autonomously synchronize thermal and mechanical signals applied to mesenchymal stem cells (MSC5). Using a grid on its underside, the shape change of polymer sheet, as well as cell morphology, calcium (Ca2+) influx, and focal adhesion assembly, could be visualized and quantified. This paper gives compelling evidence that the temperature sensing and mechanosensing of MSC5 are interconnected via intracellular Ca2+. Up-regulated Ca2+ levels lead to a remarkable alteration of histone H3K9 acetylation and activation of osteogenic related genes. The interplay of physical, thermal, and biochemical signaling was utilized to accelerate the cell differentiation toward osteogenic lineage. The approach of programmable bioinstructivity provides a fundamental principle for functional biomaterials exhibiting multifaceted stimuli on differentiation programs. Technological impact is expected in the tissue engineering of periosteum for treating bone defects.},
  language  = {en}
}
@article{EbelBald2022,
  author    = {Ebel, Kenny and Bald, Ilko},
  title     = {Low-energy (5-20 eV) electron-induced single and double strand breaks in well-defined DNA sequences},
  series = {Journal of physical chemistry letters},
  volume    = {13},
  journal   = {Journal of physical chemistry letters},
  number    = {22},
  publisher = {American Chemical Society},
  address   = {Washington, DC},
  issn      = {1948-7185},
  doi       = {10.1021/acs.jpclett.2c00684},
  pages     = {4871 -- 4876},
  year      = {2022},
  abstract  = {Ionizing radiation is used in cancer radiation therapy to effectively damage the DNA of tumors. The main damage is due to generation of highly reactive secondary species such as low-energy electrons (LEEs). The accurate quantification of DNA radiation damage of well-defined DNA target sequences in terms of absolute cross sections for LEE-induced DNA strand breaks is possible by the DNA origami technique; however, to date, it is possible only for DNA single strands. In the present work DNA double strand breaks in the DNA sequence 5 '-d(CAC)4/5 ' d(GTG)4 are compared with DNA single strand breaks in the oligonucleotides 5 '-d(CAC)4 and 5 '-d(GTG)4 upon irradiation with LEEs in the energy range from 5 to 20 eV. A maximum of strand break cross section was found around 7 and 10 eV independent of the DNA sequence, indicating that dissociative electron attachment is the underlying mechanism of strand breakage and confirming previous studies using plasmid DNA.},
  language  = {en}
}
@article{UhlemannBansseLudwigetal.1995,
  author    = {Uhlemann, Erhard and Banße, Wolfgang and Ludwig, Eberhard and Schilde, Uwe and Weller, Frank and Lehmann, Andreas},
  title     = {Ligandenaustauschreaktionen von Bis(acetylacetonato)dioxo-molybd{\"a}n(VI). Kristallstrukturen von Salicyl- aldehyd-benzoylhydrazonato(2-)]dioxom ethanol-molybd{\"a}n(VI) und [Benzoylacetonbenzoylhydrazonato(2)]dioxo- triphenylphosphan-oxidmolybd{\"a}n(VI)},
  year      = {1995},
  language  = {de}
}
@article{UhlemannBansseLudwigetal.1995,
  author    = {Uhlemann, Erhard and Banße, Wolfgang and Ludwig, Eberhard and Mickler, Wulfhard and Hahn, Ekkehardt and L{\"u}gger, Thomas and Lehmann, Andreas},
  title     = {Mangan(IV)-Komplexe mit dreiz{\"a}hnigen diaciden Liganden : Kristallstruktur von Acetylacetonato- salicylaldehyd-benzoylhydrazonato(2-methanol-mangan(III)},
  year      = {1995},
  language  = {de}
}
@article{UhlemannBansseLudwigetal.1994,
  author    = {Uhlemann, Erhard and Banße, Wolfgang and Ludwig, Eberhard and Schilde, Uwe and Weller, Frank and Lehmann, Andreas},
  title     = {Template-Reaktion von Bis(acetylacetonato)-dioxo-molybd{\"a}n(VI) mit Benzoylhydrazin},
  year      = {1994},
  language  = {de}
}
@article{BansseFliegnerHennigetal.1997,
  author    = {Banße, Wolfgang and Fliegner, Jana Ute and Hennig, H. and Lehmann, Andreas and Uhlemann, Erhard and Tschwatschal, F.},
  title     = {Ligand exchange reactions of molybdenum hexacarbonyl with tridentate diacidic ligands},
  year      = {1997},
  language  = {en}
}
@article{HermannsKunold2022,
  author    = {Hermanns, Jolanda and Kunold, Helen},
  title     = {Mechanism comics as a task in a written exam in organic chemistry for pre-service chemistry teachers},
  series = {Chemistry teacher international : best practices in chemistry education},
  volume    = {4},
  journal   = {Chemistry teacher international : best practices in chemistry education},
  number    = {3},
  publisher = {De Gruyter},
  address   = {Berlin},
  issn      = {2569-3263},
  doi       = {10.1515/cti-2021-0035},
  pages     = {259 -- 269},
  year      = {2022},
  abstract  = {In this paper, we describe and evaluate a study on the use of mechanism comics for writing solutions to a task in a written exam for the course "Organic Chemistry I for Pre-Service Chemistry Teachers." The students had to design a reaction mechanism for a reaction that was unknown to them and write captions explaining every step of their reaction mechanism. The students' work was evaluated using the method of qualitative content analysis in four rounds by both authors. The majority of the captions were coded as "descriptive" and only a minority as "causal." This means that the students mostly described "what" happened, but seldom "why" this happened. Implicit electron movement was also described more often than explicit electron movement. The majority of the captions were technically correct. In summary, the students were capable of designing and describing a reaction mechanism for a previously unknown reaction. The quality of their reasoning could be improved, however. In the new course, the quality of students' mechanistic reasoning and then especially their explanations of "why" mechanistic steps occur will be given much clearer emphasis.},
  language  = {en}
}
@article{VorburgerNedielkovBrosigetal.2016,
  author    = {Vorburger, Thomas and Nedielkov, Ruslan and Brosig, Alexander and Bok, Eva and Schunke, Emina and Steffen, Wojtek and Mayer, Sonja and Goetz, Friedrich and M{\"o}ller, Heiko Michael and Steuber, Julia},
  title     = {Role of the Na+-translocating NADH:quinone oxidoreductase in voltage generation and Na+ extrusion in Vibrio cholerae},
  series = {Biochimica et biophysica acta : Bioenergetics},
  volume    = {1857},
  journal   = {Biochimica et biophysica acta : Bioenergetics},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {0005-2728},
  doi       = {10.1016/j.bbabio.2015.12.010},
  pages     = {473 -- 482},
  year      = {2016},
  abstract  = {For Vibrio cholerae, the coordinated import and export of Na+ is crucial for adaptation to habitats with different osmolarities. We investigated the Na+-extruding branch of the sodium cycle in this human pathogen by in vivo Na-23-NMR spectroscopy. The Na+ extrusion activity of cells was monitored after adding glucose which stimulated respiration via the Na+-translocating NADH:quinone oxidoreductase (Na+-NQR). In a V. cholerae deletion mutant devoid of the Na+-NQR encoding genes (nqrA-F), rates of respiratory Na+ extrusion were decreased by a factor of four, but the cytoplasmic Na+ concentration was essentially unchanged. Furthermore, the mutant was impaired in formation of transmembrane voltage (Delta psi, inside negative) and did not grow under hypoosmotic conditions at pH 8.2 or above. This growth defect could be complemented by transformation with the plasmid encoded nqr operon. In an alkaline environment, Na+/H+ antiporters acidify the cytoplasm at the expense of the transmembrane voltage. It is proposed that, at alkaline pH and limiting Na+ concentrations, the Na+-NQR is crucial for generation of a transmembrane voltage to drive the import of H+ by electrogenic Na+/H+ antiporters. Our study provides the basis to understand the role of the Na+-NQR in pathogenicity of V. cholerae and other pathogens relying on this primary Na+ pump for respiration. (C) 2015 Elsevier B.V. All rights reserved.},
  language  = {en}
}
@inproceedings{RamadanGuerreroNedielkovetal.2021,
  author    = {Ramadan, Shahenda and Guerrero, Paula and Nedielkov, Ruslan and Klishin, Nikolai and Dimova, Rumiana and Silva, Daniel V. and M{\"o}ller, Heiko},
  title     = {Building a mimetic system for unraveling protein-protein interactions on membranes},
  series = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  volume    = {50},
  booktitle = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  number    = {SUPPL 1},
  publisher = {Springer},
  address   = {Berlin ; Heidelberg ; New York},
  issn      = {0175-7571},
  doi       = {10.1007/s00249-021-01558-w},
  pages     = {S153 -- S153},
  year      = {2021},
  language  = {en}
}
@article{PruefertBeitzReichetal.2022,
  author    = {Pr{\"u}fert, Christian and Beitz, Toralf and Reich, Olaf and L{\"o}hmannsr{\"o}ben, Hans-Gerd},
  title     = {Inline process analysis of copper-bearing aerosols using laser-induced breakdown spectroscopy, laser-induced incandescence and optical imaging},
  series = {Spectrochimica acta, Part B, Atomic spectroscopy},
  volume    = {197},
  journal   = {Spectrochimica acta, Part B, Atomic spectroscopy},
  publisher = {Elsevier},
  address   = {Amsterdam [u.a.]},
  issn      = {0584-8547},
  doi       = {10.1016/j.sab.2022.106527},
  pages     = {11},
  year      = {2022},
  abstract  = {The quantification and identification of aerosols in industry plays a key role in process monitoring and control and lays the foundation for process automation aspired by the industry 4.0 initiative. However, measuring particulate matter's mass and number concentrations in harsh environments poses great analytical constraints. The presented approach comprises a comprehensive set of light-and imaging-based techniques, all contactless, in-line, and real-time. It includes, but is not limited to, stroboscopic imaging, laser-induced breakdown spectroscopy (LIBS) and laser-induced incandescence (LII). Stroboscopic imaging confirmed the particles sphericity and was used to measure the particle number density. A phase-selective LIBS setup with low fluence and 500 Hz repetition rate was used to classify each particle with a single-pulse and in real time. Simultaneously, the created plasma was captured by CCD imaging to determine the detection volume and hit rate of the LIBS setup. Both data sets combined were converted to a particle number density, which was consistent with the particle number density of the stroboscopic measurements. Furthermore, using a photodiode and microphone in parallel to the LIBS setup allowed for the photoacoustic normalization of the spectral line intensity at the laser repetition rate of 500 Hz. This was done as a partial photoacoustic normalization method with the cut-off based on the coefficient of variation (CV), reducing it by 25\%. Aside from that photodiode and microphone were proven to be valuable event counting with the advantage of the less spatially constricted. A second laser setup was used for laser -induced incandescence (LII) making it possible to classify the particles based on their incandescence tendency. Given its larger probing volume, LII could be employed at very low particle number densities. With respect to the current literature, this is the first approach of using LII as an in-line, real-time analytical technique for the compositional classification of metal-bearing aerosols.},
  language  = {en}
}
@article{WessigBadetkoKoebe2022,
  author    = {Wessig, Pablo and Badetko, Dominik and Koebe, Michael},
  title     = {Triplet sensitized Photo-Dehydro-Diels-Alder reaction},
  series = {ChemistrySelect},
  volume    = {7},
  journal   = {ChemistrySelect},
  number    = {38},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {2365-6549},
  doi       = {10.1002/slct.202202648},
  pages     = {6},
  year      = {2022},
  abstract  = {We report on the triplet sensitization of the intramolecular Photo-Dehydro-Diels-Alder (PDDA) reaction of two diaryl suberates bearing methyl propiolate chromophors. Compared with the non-sensitized irradiation, considerably increased yields could be observed. Moreover, it is possible to use the more efficient UVA lamps instead of UVB lamps. Among three investigated sensitizers (xanthone, benzophenone, thioxanthone) xanthone gave the best results.},
  language  = {en}
}
@phdthesis{Martin2024,
  author    = {Martin, Johannes},
  title     = {Synthesis of protein-polymer conjugates and block copolymers via sortase-mediated ligation},
  doi       = {10.25932/publishup-64566},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-645669},
  school      = {Universit{\"a}t Potsdam},
  pages     = {XVII, 150},
  year      = {2024},
  abstract  = {In den vergangenen Jahrzehnten haben therapeutische Proteine in der pharmazeutischen Industrie mehr und mehr an Bedeutung gewonnen. Werden Proteine nichtmenschlichen Ursprungs verwendet, kann es jedoch zu einer Immunreaktion kommen, sodass das Protein sehr schnell aus dem K{\"o}rper ausgeschieden oder abgebaut wird. Um die Zirkulationszeit im Blut signifikant zu verl{\"a}ngern, werden die Proteine mit synthetischen Polymeren modifiziert (Protein-Polymer-Konjugate). Die Proteine aller heute auf dem Markt erh{\"a}ltlichen Medikamente dieser Art tragen eine oder mehrere Polymerketten aus Poly(ethylenglycol) (PEG). Ein Nachteil der PEGylierung ist, dass viele Patienten bei regelm{\"a}ßiger Einnahme dieser Medikamente Antik{\"o}rper gegen PEG entwickeln, die den effizienzsteigernden Effekt der PEGylierung wieder aufheben. Ein weiterer Nachteil der PEGylierung ist die oftmals deutlich verringerte Aktivit{\"a}t der Konjugate im Vergleich zum nativen Protein. Der Grund daf{\"u}r ist die Herstellungsmethode der Konjugate, bei der meist die prim{\"a}ren Amine der Lysin-Seitenketten und der N-Terminus des Proteins genutzt werden. Da die meisten Proteine mehrere gut zug{\"a}ngliche Lysine aufweisen, werden oft unterschiedliche und teilweise mehrere Lysine mit PEG funktionalisiert, was zu einer Mischung an Regioisomeren f{\"u}hrt. Je nach Position der PEG-Kette kann das aktive Zentrum abgeschirmt oder die 3D-Struktur des Proteins ver{\"a}ndert werden, was zu einem teilweise drastischen Aktivit{\"a}tsabfall f{\"u}hrt. In dieser Arbeit wurde eine neuartige Methode zur Ligation von Makromolek{\"u}len untersucht. Die Verwendung eines Enzyms als Katalysator zur Verbindung zweier Makromolek{\"u}le ist bisher wenig untersucht und ineffizient. Als Enzym wurde Sortase A ausgew{\"a}hlt, eine gut untersuchte Ligase aus der Familie der Transpeptidasen, welche die Ligation zweier Peptide katalysieren kann. Ein Nachteil dieser Sortase-vermittelten Ligation ist, dass es sich um eine Gleichgewichtsreaktion handelt, wodurch hohe Ausbeuten schwierig zu erreichen sind. Im Rahmen dieser Dissertation wurden zwei zuvor entwickelte Methoden zur Verschiebung des Gleichgewichts ohne Einsatz eines großen {\"U}berschusses von einem Edukt f{\"u}r Makromolek{\"u}le {\"u}berpr{\"u}ft. Zur Durchf{\"u}hrung der Sortase-vermittelten Ligation werden zwei komplement{\"a}re Peptidsequenzen verwendet, die Erkennungssequenz und das Nukleophil. Um eine systematische Untersuchung durchf{\"u}hren zu k{\"o}nnen, wurden alle n{\"o}tigen Bausteine (Protein-Erkennungssequenz zur Reaktion mit Nukleophil-Polymer und Polymer-Erkennungssequenz mit Nukleophil-Protein) hergestellt. Als Polymerisationstechnik wurde die radikalische Polymerisation mit reversibler Deaktivierung (im Detail, Atom Transfer Radical Polymerization, ATRP und Reversible Addition-Fragmentation Chain Transfer, RAFT polymerization) gew{\"a}hlt, um eine enge Molmassenverteilung zu erreichen. Die Herstellung der Bausteine begann mit der Synthese der Peptide via automatisierter Festphasen-Peptidsynthese, um eine einfache {\"A}nderung der Peptidsequenz zu gew{\"a}hrleisten und um eine Modifizierung der Polymerkette nach der Polymerisation zu umgehen. Um die ben{\"o}tigte unterschiedliche Funktionalit{\"a}t der zwei Peptidsequenzen (freier C-Terminus bei der Erkennungssequenz bzw. freier N-Terminus bei dem Nukleophil) zu erreichen, wurden verschiedene Linker zwischen Harz und Peptid verwendet. Danach wurde der Ketten{\"u}bertr{\"a}ger (chain transfer agent, CTA) zur Kontrolle der Polymerisation mit dem auf dem Harz befindlichen Peptid gekoppelt. Die f{\"u}r die anschließende Polymerisation verwendeten Monomere basierten auf Acrylamiden und Acrylaten und wurden anhand ihrer Eignung als Alternativen zu PEG ausgew{\"a}hlt. Es wurde eine k{\"u}rzlich entwickelte Technik basierend auf der RAFT-Polymerisation (xanthate-supported photo-iniferter RAFT, XPI-RAFT) verwendet um eine Reihe an Peptid-Polymeren mit unterschiedlichen Molekulargewichten und engen Molekulargewichtsverteilungen herzustellen. Nach Entfernung der Schutzgruppen der Peptid-Seitenketten wurden die Peptid-Polymere zun{\"a}chst genutzt, um mittels Sortase-vermittelter Ligation zwei Polymerketten zu einem Blockcopolymer zu verbinden. Unter Verwendung von Ni2+-Ionen in Kombination mit einer Verl{\"a}ngerung der Erkennungssequenz um ein Histidin zur Unterdr{\"u}ckung der R{\"u}ckreaktion konnte ein maximaler Umsatz von 70 \% erreicht werden. Dabei zeigte sich ein oberes Limit von durchschnittlich 100 Wiederholungseinheiten; die Ligation von l{\"a}ngeren Polymeren war nicht erfolgreich. Danach wurden ein Modellprotein und ein Nanobody mit vielversprechenden medizinischen Eigenschaften mit den f{\"u}r die enzymkatalysierte Ligation ben{\"o}tigten Peptidsequenzen f{\"u}r die Kopplung mit den zuvor hergestellten Peptid-Polymeren verwendet. Dabei konnte bei Verwendung des Modellproteins keine Bildung von Protein-Polymer-Konjugaten beobachtet werden. Der Nanobody konnte dagegen C-terminal mit einem Polymer funktionalisiert werden. Dabei wurde eine {\"a}hnliche Limitierung in der Polymer-Kettenl{\"a}nge beobachtet wie zuvor. Die auf Ni-Ionen basierte Strategie zur Gleichgewichtsverschiebung hatte hier keinen ausschlaggebenden Effekt, w{\"a}hrend die Verwendung von einem {\"U}berschuss an Polymer zur vollst{\"a}ndigen Umsetzung des Edukt-Nanobody f{\"u}hrte. Die erhaltenen Daten aus diesem Projekt bilden eine gute Basis f{\"u}r weitere Forschung in dem vielversprechenden Feld der enzymkatalysierten Herstellung von Protein-Polymer-Konjugaten und Blockcopolymeren. Langfristig k{\"o}nnte diese Herangehensweise eine vielseitig einsetzbare Herstellungsmethode von ortsspezifischen therapeutischen Protein-Polymer Konjugaten darstellen, welche sowohl eine hohe Aktivit{\"a}t als auch eine lange Zirkulationszeit im Blut aufweisen.},
  language  = {en}
}
@article{Titov2022,
  author    = {Titov, Evgenii},
  title     = {Effect of conformational disorder on exciton states of an azobenzene aggregate},
  series = {Physical chemistry, chemical physics : a journal of European Chemical Societies},
  volume    = {24},
  journal   = {Physical chemistry, chemical physics : a journal of European Chemical Societies},
  number    = {39},
  publisher = {Royal Society of Chemistry},
  address   = {Cambridge},
  issn      = {1463-9076},
  doi       = {10.1039/d2cp02774g},
  pages     = {24002 -- 24006},
  year      = {2022},
  abstract  = {Azobenzene is a prototypical molecular photoswitch, widely used to trigger a variety of transformations at different length scales. In systems like self-assembled monolayers or micelles, azobenzene chromophores may interact with each other, which gives rise to the emergence of exciton states. Here, using first-principles calculations, we investigate how conformational disorder (induced, e.g., by thermal fluctuations) affects localization of these states, on an example of an H-type azobenzene tetramer. We find that conformational disorder leads to (partial) exciton localization on a single-geometry level, whereas ensemble-averaging results in a delocalized picture. The pi pi* and n pi* excitons at high and low temperatures are discussed.},
  language  = {en}
}
@article{SandSchmidt2022,
  author    = {Sand, Patrick and Schmidt, Bernd},
  title     = {Orthogonal arylation of a diene-sulfonamide using cationic transition metal catalysts},
  series = {European journal of organic chemistry},
  volume    = {2022},
  journal   = {European journal of organic chemistry},
  number    = {47},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {1434-193X},
  doi       = {10.1002/ejoc.202201336},
  pages     = {8},
  year      = {2022},
  abstract  = {The regioselectivity of two mechanistically distinct alkenylation reactions catalyzed by in situ-formed cationic transition metal complexes was studied using N-allyl-N-phenylethenesulfonamide as a model compound. Orthogonal selectivity was observed for the Ru-catalyzed C-H-activating alkenylation with acetanilides, which occurs preferentially at the electron deficient double bond, and for a Pd-catalyzed Heck-type coupling with arene diazonium salts, which occurs preferentially at the more electron rich double bond of the N-allyl substituent.},
  language  = {en}
}
@article{LehnenKurkiHartlieb2022,
  author    = {Lehnen, Anne-Catherine and Kurki, Jan A. M. and Hartlieb, Matthias},
  title     = {The difference between photo-iniferter and conventional RAFT polymerization},
  series = {Polymer Chemistry},
  volume    = {13},
  journal   = {Polymer Chemistry},
  number    = {11},
  publisher = {Royal Society of Chemistry},
  address   = {Cambridge},
  issn      = {1759-9954},
  doi       = {10.1039/d1py01530c},
  pages     = {1537 -- 1546},
  year      = {2022},
  abstract  = {Photo-iniferter (PI)-RAFT polymerization, the direct activation of chain transfer agents via light, is a fascinating polymerization technique, as it overcomes some restriction of conventional RAFT polymerization. As such, we elucidated the role of reversible deactivation in this context using a monomer-CTA pair with low chain transfer capabilities. Tests with varying targeted degrees of polymerization (DP) or monomer concentrations revealed no significant improvement of polymerization control using the PI-process. Control can however be achieved via slow monomer addition, increasing the number of activation/deactivation events per monomer addition. More importantly, the livingness of the polymerization was found to be extraordinarily high, enabling the straightforward and rapid synthesis of multiblock copolymers with up to 20 blocks and a high number of repeating units per block (DP = 25-100) maintaining an overall excellent definition (M-n = 90 300 g mol(-1), D = 1.29). This study highlights the enormous potential of PI-RAFT polymerization for the synthesis of polymeric materials.},
  language  = {en}
}
@article{OliverLunnUrbanczykWochniaketal.2008,
  author    = {Oliver, Sandra N. and Lunn, John Edward and Urbanczyk-Wochniak, Ewa and Lytovchenko, Anna and van Dongen, Joost T. and Faix, Benjamin and Schm{\"a}lzlin, Elmar and Fernie, Alisdair and Schm{\"a}elzlin, E. and Geigenberger, Peter},
  title     = {Decreased expression of cytosolic pyruvate kinase in potato tubers leads to a decline in pyruvate resulting in an in vivo repression of the alternative oxidase},
  doi       = {10.1104/pp.108.126516},
  year      = {2008},
  abstract  = {The aim of this work was to investigate the effect of decreased cytosolic pyruvate kinase (PKc) on potato (Solanum tuberosum) tuber metabolism. Transgenic potato plants with strongly reduced levels of PKc were generated by RNA interference gene silencing under the control of a tuber-specific promoter. Metabolite profiling showed that decreased PKc activity led to a decrease in the levels of pyruvate and some other organic acids involved in the tricarboxylic acid cycle. Flux analysis showed that this was accompanied by changes in carbon partitioning, with carbon flux being diverted from glycolysis toward starch synthesis. However, this metabolic shift was relatively small and hence did not result in enhanced starch levels in the tubers. Although total respiration rates and the ATP to ADP ratio were largely unchanged, transgenic tubers showed a strong decrease in the levels of alternative oxidase (AOX) protein and a corresponding decrease in the capacity of the alternative pathway of respiration. External feeding of pyruvate to tuber tissue or isolated mitochondria resulted in activation of the AOX pathway, both in the wild type and the PKc transgenic lines, providing direct evidence for the regulation of AOX by changes in pyruvate levels. Overall, these results provide evidence for a crucial role of PKc in the regulation of pyruvate levels as well as the level of the AOX in heterotrophic plant tissue, and furthermore reveal that these parameters are interlinked in vivo.},
  language  = {en}
}
@article{BastianRobelSchmidtetal.2021,
  author    = {Bastian, Philipp U. and Robel, Nathalie and Schmidt, Peter and Schrumpf, Tim and G{\"u}nter, Christina and Roddatis, Vladimir and Kumke, Michael Uwe},
  title     = {Resonance energy transfer to track the motion of lanthanide ions},
  series = {Biosensors : open access journal},
  volume    = {11},
  journal   = {Biosensors : open access journal},
  number    = {12},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {2079-6374},
  doi       = {10.3390/bios11120515},
  pages     = {23},
  year      = {2021},
  abstract  = {The imagination of clearly separated core-shell structures is already outdated by the fact, that the nanoparticle core-shell structures remain in terms of efficiency behind their respective bulk material due to intermixing between core and shell dopant ions. In order to optimize the photoluminescence of core-shell UCNP the intermixing should be as small as possible and therefore, key parameters of this process need to be identified. In the present work the Ln(III) ion migration in the host lattices NaYF4 and NaGdF4 was monitored. These investigations have been performed by laser spectroscopy with help of lanthanide resonance energy transfer (LRET) between Eu(III) as donor and Pr(III) or Nd(III) as acceptor. The LRET is evaluated based on the Forster theory. The findings corroborate the literature and point out the migration of ions in the host lattices. Based on the introduced LRET model, the acceptor concentration in the surrounding of one donor depends clearly on the design of the applied core-shell-shell nanoparticles. In general, thinner intermediate insulating shells lead to higher acceptor concentration, stronger quenching of the Eu(III) donor and subsequently stronger sensitization of the Pr(III) or the Nd(III) acceptors. The choice of the host lattice as well as of the synthesis temperature are parameters to be considered for the intermixing process.},
  language  = {en}
}
@article{DettmannHuittinenNicolasetal.2023,
  author    = {Dettmann, Sophie and Huittinen, Nina Maria and Nicolas, Jahn and Kretzschmar, Jerome and Kumke, Michael Uwe and Kutyma, Tamara and Lohmann, Janik and Reich, Tobias and Schmeide, Katja and Azzam, Salim Shams Aldin and Spittler, Leon and Stietz, Janina},
  title     = {Influence of gluconate on the retention of Eu(III), Am(III), Th(IV), Pu(IV), and U(VI) by C-S-H (C/S = 0.8)},
  series = {Frontiers in Nuclear Engineering},
  volume    = {2},
  journal   = {Frontiers in Nuclear Engineering},
  publisher = {Frontiers Media},
  address   = {Lausanne},
  issn      = {2813-3412},
  doi       = {10.3389/fnuen.2023.1124856},
  pages     = {15},
  year      = {2023},
  abstract  = {The retention of actinides in different oxidation states (An(X), X = III, IV, VI) by a calcium-silicate-hydrate (C-S-H) phase with a Ca/Si (C/S) ratio of 0.8 was investigated in the presence of gluconate (GLU). The actinides considered were Am(III), Th(IV), Pu(IV), and U(VI). Eu(III) was investigated as chemical analogue for Am(III) and Cm(III). In addition to the ternary systems An(X)/GLU/C-S-H, also binary systems An(X)/C-S-H, GLU/C-S-H, and An(X)/GLU were studied. Complementary analytical techniques were applied to address the different specific aspects of the binary and ternary systems. Time-resolved laser-induced luminescence spectroscopy (TRLFS) was applied in combination with parallel factor analysis (PARAFAC) to identify retained species and to monitor species-selective sorption kinetics. ¹³C and ²⁹Si magic-angle-spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy and X-ray photoelectron spectroscopy (XPS) were applied to determine the bulk structure and the composition of the C-S-H surface, respectively, in the absence and presence of GLU. The interaction of Th(IV) with GLU in different electrolytes was studied by capillary electrophoresis-inductively coupled plasma mass spectrometry (CE-ICP-MS). The influence of GLU on An(X) retention was investigated for a large concentration range up to 10⁻² M. The results showed that GLU had little to no effect on the overall An(X) retention by C-S-H with C/S of 0.8, regardless of the oxidation state of the actinides. For Eu(III), the TRLFS investigations additionally implied the formation of a Eu(III)-bearing precipitate with dissolved constituents of the C-S-H phase, which becomes structurally altered by the presence of GLU. For U(VI) sorption on the C-S-H phase, only a small influence of GLU could be established in the luminescence spectroscopic investigations, and no precipitation of U(VI)-containing secondary phases could be identified.},
  language  = {en}
}
@misc{DettmannHuittinenJahnetal.2023,
  author    = {Dettmann, Sophie and Huittinen, Nina Maria and Jahn, Nicolas and Kretzschmar, Jerome and Kumke, Michael Uwe and Kutyma, Tamara and Lohmann, Janik and Reich, Tobias and Schmeide, Katja and Azzam, Salim Shams Aldin and Spittler, Leon and Stietz, Janina},
  title     = {Influence of gluconate on the retention of Eu(III), Am(III), Th(IV), Pu(IV), and U(VI) by C-S-H (C/S = 0.8)},
  series = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  journal   = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  number    = {1318},
  issn      = {1866-8372},
  doi       = {10.25932/publishup-58845},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-588455},
  pages     = {15},
  year      = {2023},
  abstract  = {The retention of actinides in different oxidation states (An(X), X = III, IV, VI) by a calcium-silicate-hydrate (C-S-H) phase with a Ca/Si (C/S) ratio of 0.8 was investigated in the presence of gluconate (GLU). The actinides considered were Am(III), Th(IV), Pu(IV), and U(VI). Eu(III) was investigated as chemical analogue for Am(III) and Cm(III). In addition to the ternary systems An(X)/GLU/C-S-H, also binary systems An(X)/C-S-H, GLU/C-S-H, and An(X)/GLU were studied. Complementary analytical techniques were applied to address the different specific aspects of the binary and ternary systems. Time-resolved laser-induced luminescence spectroscopy (TRLFS) was applied in combination with parallel factor analysis (PARAFAC) to identify retained species and to monitor species-selective sorption kinetics. ¹³C and ²⁹Si magic-angle-spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy and X-ray photoelectron spectroscopy (XPS) were applied to determine the bulk structure and the composition of the C-S-H surface, respectively, in the absence and presence of GLU. The interaction of Th(IV) with GLU in different electrolytes was studied by capillary electrophoresis-inductively coupled plasma mass spectrometry (CE-ICP-MS). The influence of GLU on An(X) retention was investigated for a large concentration range up to 10⁻² M. The results showed that GLU had little to no effect on the overall An(X) retention by C-S-H with C/S of 0.8, regardless of the oxidation state of the actinides. For Eu(III), the TRLFS investigations additionally implied the formation of a Eu(III)-bearing precipitate with dissolved constituents of the C-S-H phase, which becomes structurally altered by the presence of GLU. For U(VI) sorption on the C-S-H phase, only a small influence of GLU could be established in the luminescence spectroscopic investigations, and no precipitation of U(VI)-containing secondary phases could be identified.},
  language  = {en}
}
@article{ChemuraSchrumpfGuenteretal.2023,
  author    = {Chemura, Sitshengisiwe and Schrumpf, Tim and G{\"u}nter, Christina and Kumke, Michael Uwe},
  title     = {Ceria nanomaterials containing ytterbium},
  series = {RSC Advances : an international journal to further the chemical sciences},
  volume    = {13},
  journal   = {RSC Advances : an international journal to further the chemical sciences},
  number    = {50},
  publisher = {RSC Publishing},
  address   = {London},
  issn      = {2046-2069},
  doi       = {10.1039/D3RA06868D},
  pages     = {35445 -- 35456},
  year      = {2023},
  abstract  = {Lanthanide based ceria nanomaterials are important practical materials due to the redox properties that are useful in the avenues pertaining to technology and life sciences. Sub 10 nm spherical and highly monodisperse Ce1-xYbxO2-y (0.04 ≤ x ≤ 0.22) nanoparticles were synthesized by thermal decomposition, annealed separately at 773 K and 1273 K for 2 hours and characterized. Elemental mapping for Yb3+ doped ceria nanoparticles shows homogeneous distribution of Yb3+ atoms in the ceria with low Yb3+ content annealed at 773 K and 1273 K for 2 hours. However, clusters are observed for 773 K annealed ceria samples with high concentration of Yb3+. These clusters are not detected in 1273 K annealed nanomaterials. Introducing small amounts of Yb3+ ions into the ceria lattice as spectroscopic probes can provide detailed information about the atomic structure and local environments allowing the monitoring of small structural changes, such as clustering. The emission spectra observed at room temperature and at 4 K have a manifold of bands that corresponds to the 2F5/2 → 2F7/2 transition of Yb3+ ions. Some small shifts are observed in the Stark splitting pattern depending on the sample and the annealing conditions. The deconvolution by PARAFAC analysis yielded luminescence decay kinetics as well as the associated luminescence spectra of three species for each of the low Yb3+ doped ceria samples annealed at 773 K and one species for the 1273 K annealed samples. However, the ceria samples with high concentration of Yb3+ annealed at the two temperatures showed only one species with lower decay times as compared to the low Yb3+ doped ceria samples.},
  language  = {en}
}
@phdthesis{Chemura2023,
  author    = {Chemura, Sitshengisiwe},
  title     = {Optical spectroscopy on lanthanide-modified nanomaterials for performance monitoring},
  doi       = {10.25932/publishup-61944},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-619443},
  school      = {Universit{\"a}t Potsdam},
  pages     = {xi, 116},
  year      = {2023},
  abstract  = {Lanthanide based ceria nanomaterials are important practical materials due to their redox properties that are useful in technology and life sciences. This PhD thesis examined various properties and potential for catalytic and bio-applications of Ln3+-doped ceria nanomaterials. Ce1-xGdxO2-y: Eu3+, gadolinium doped ceria (GDC) (0 ≤ x ≤ 0.4) nanoparticles were synthesized by flame spray pyrolysis (FSP) and studied, followed by 15 \% CexZr1-xO2-y: Eu3+|YSZ (0 ≤ x ≤ 1) nanocomposites. Furthermore, Ce1-xYb xO2-y (0.004 ≤ x ≤ 0.22) nanoparticles were synthesized by thermal decomposition and characterized. Finally, CeO2-y: Eu3+ nanoparticles were synthesized by a microemulsion method, biofunctionalized and characterized. The studies undertaken presents a novel approach to structurally elucidate ceria-based nanomaterials by way of Eu3+ and Yb3+ spectroscopy and processing the spectroscopic data with the multi-way decomposition method PARAFAC. Data sets of the three variables: excitation wavelength, emission wavelength and time were used to perform the deconvolution of spectra. GDC nanoparticles from FSP are nano-sized and of roughly cubic shape and crystal structure (Fm3̅m). Raman data revealed four vibrational modes exhibited by Gd3+ containing samples whereas CeO2-y: Eu3+ displays only two. The room temperature, time-resolved emission spectra recorded at λexcitation = 464 nm show that Gd3+ doping results in significantly altered emission spectra compared to pure ceria. The PARAFAC analysis for the pure ceria samples reveals two species; a high-symmetry species and a low-symmetry species. The GDC samples yield two low-symmetry spectra in the same experiment. High-resolution emission spectra recorded at 4 K after probing the 5D0-7F0 transition revealed additional variation in the low symmetry Eu3+ sites in pure ceria and GDC. The data of the Gd3+-containing samples indicates that the average charge density around the Eu3+ ions in the lattice is inversely related to Gd3+ and oxygen vacancy concentration. The particle crystallites of the 773 K and 1273 K annealed Yb3+ -ceria nanostructure materials are nano-sized and have a cubic fluorite structure with four Raman vibrational modes. Elemental maps clearly show that cluster formation occurs for 773 K annealed with high Yb3+ ion concentration from 15 mol \% in the ceria lattice. These clusters are destroyed with annealing to 1273 K. The emission spectra observed from room temperature and 4 K measurements for the Ce1-xYb xO2-y samples have a manifold that corresponds to the 2F5/2-2F7/2 transition of Yb3+ ions. Some small shifts are observed in the Stark splitting pattern and are induced by the variations of the crystal field influenced by where the Yb3+ ions are located in the crystal lattices in the samples. Upon mixing ceria with high Yb3+ concentrations, the 2F5/2-2F7/2 transition is also observed in the Stark splitting pattern, but the spectra consist of two broad high background dominated peaks. Annealing the nanomaterials at 1273 K for 2 h changes the spectral signature as new peaks emerge. The deconvolution yielded luminescence decay kinetics as well as the accompanying luminescence spectra of three species for each of the low Yb3+ doped ceria samples annealed at 773 K and one species for the 1273 K annealed samples. However, the ceria samples with high Yb3+ concentration annealed at the two temperatures yielded one species with lower decay times as compared to the Yb3+ doped ceria samples after PARAFAC analysis. Through the calcination of the nanocomposites at two high temperatures, the evolution of the emission patterns from specific Eu3+ lattice sites to indicate structural changes for the nanocomposites was followed. The spectroscopy results effectively complemented the data obtained from the conventional techniques. Annealing the samples at 773 K, resulted in amorphous, unordered domains whereas the TLS of the 1273 K nanocomposites reveal two distinct sites, with most red shifted Eu3+ species coming from pure Eu3+ doped ZrO2 on the YSZ support. Finally, for Eu3+ doped ceria, successful transfer from hydrophobic to water phase and subsequent biocompatibility was achieved using ssDNA. PARAFAC analysis for the Eu3+ in nanoparticles dispersed in toluene and water revealed one Eu3+ species, with slightly differing surface properties for the nanoparticles as far as the luminescence kinetics and solvent environments were concerned. Several functionalized nanoparticles conjugated onto origami triangles after hybridization were visualized by atomic force microscopy (AFM). Putting all into consideration, Eu3+ and Yb3+ spectroscopy was used to monitor the structural changes and determining the feasibility of the nanoparticle transfer into water. PARAFAC proves to be a powerful tool to analyze lanthanide spectra in crystalline solid materials and in solutions, which are characterized by numerous Stark transitions and where measurements usually yield a superposition of different emission contributions to any given spectrum.},
  language  = {en}
}
@misc{BlockGuenterDuarteRodriguesetal.2021,
  author    = {Block, Inga and G{\"u}nter, Christina and Duarte Rodrigues, Alysson and Paasch, Silvia and Hesemann, Peter and Taubert, Andreas},
  title     = {Carbon Adsorbents from Spent Coffee for Removal of Methylene Blue and Methyl Orange from Water},
  series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  journal   = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  number    = {14},
  issn      = {1866-8372},
  doi       = {10.25932/publishup-52165},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-521653},
  pages     = {20},
  year      = {2021},
  abstract  = {Activated carbons (ACs) were prepared from dried spent coffee (SCD), a biological waste product, to produce adsorbents for methylene blue (MB) and methyl orange (MO) from aqueous solution. Pre-pyrolysis activation of SCD was achieved via treatment of the SCD with aqueous sodium hydroxide solutions at 90 °C. Pyrolysis of the pretreated SCD at 500 °C for 1 h produced powders with typical characteristics of AC suitable and effective for dye adsorption. As an alternative to the rather harsh base treatment, calcium carbonate powder, a very common and abundant resource, was also studied as an activator. Mixtures of SCD and CaCO3 (1:1 w/w) yielded effective ACs for MO and MB removal upon pyrolysis needing only small amounts of AC to clear the solutions. A selectivity of the adsorption process toward anionic (MO) or cationic (MB) dyes was not observed.},
  language  = {en}
}
@article{BlockGuenterDuarteRodriguesetal.2021,
  author    = {Block, Inga and G{\"u}nter, Christina and Duarte Rodrigues, Alysson and Paasch, Silvia and Hesemann, Peter and Taubert, Andreas},
  title     = {Carbon adsorbents from spent coffee for removal of methylene blue and methyl orange from water},
  series = {Materials / Molecular Diversity Preservation International},
  volume    = {14},
  journal   = {Materials / Molecular Diversity Preservation International},
  number    = {14},
  publisher = {Molecular Diversity Preservation International},
  address   = {Basel},
  issn      = {1996-1944},
  doi       = {10.3390/ma14143996},
  pages     = {18},
  year      = {2021},
  abstract  = {Activated carbons (ACs) were prepared from dried spent coffee (SCD), a biological waste product, to produce adsorbents for methylene blue (MB) and methyl orange (MO) from aqueous solution. Pre-pyrolysis activation of SCD was achieved via treatment of the SCD with aqueous sodium hydroxide solutions at 90 °C. Pyrolysis of the pretreated SCD at 500 °C for 1 h produced powders with typical characteristics of AC suitable and effective for dye adsorption. As an alternative to the rather harsh base treatment, calcium carbonate powder, a very common and abundant resource, was also studied as an activator. Mixtures of SCD and CaCO3 (1:1 w/w) yielded effective ACs for MO and MB removal upon pyrolysis needing only small amounts of AC to clear the solutions. A selectivity of the adsorption process toward anionic (MO) or cationic (MB) dyes was not observed.},
  language  = {en}
}
@misc{PieperWeheBornhorstetal.2014,
  author    = {Pieper, Imke and Wehe, Christoph A. and Bornhorst, Julia and Ebert, Franziska and Leffers, Larissa and Holtkamp, Michael and H{\"o}seler, Pia and Weber, Till and Mangerich, Aswin and B{\"u}rkle, Alexander and Karst, Uwe and Schwerdtle, Tanja},
  title     = {Mechanisms of Hg species induced toxicity in cultured human astrocytes},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-74379},
  pages     = {662 -- 671},
  year      = {2014},
  abstract  = {The toxicologically most relevant mercury (Hg) species for human exposure is methylmercury (MeHg). Thiomersal is a common preservative used in some vaccine formulations. The aim of this study is to get further mechanistic insight into the yet not fully understood neurotoxic modes of action of organic Hg species. Mercury species investigated include MeHgCl and thiomersal. Additionally HgCl2 was studied, since in the brain mercuric Hg can be formed by dealkylation of the organic species. As a cellular system astrocytes were used. In vivo astrocytes provide the environment necessary for neuronal function. In the present study, cytotoxic effects of the respective mercuricals increased with rising alkylation level and correlated with their cellular bioavailability. Further experiments revealed for all species at subcytotoxic concentrations no induction of DNA strand breaks, whereas all species massively increased H2O2-induced DNA strand breaks. This co- genotoxic effect is likely due to a disturbance of the cellular DNA damage response. Thus, at nanomolar, sub-cytotoxic concentrations, all three mercury species strongly disturbed poly(ADP-ribosyl)ation, a signalling reaction induced by DNA strand breaks. Interestingly, the molecular mechanism behind this inhibition seems to be different for the species. Since chronic PARP-1 inhibition is also discussed to sacrifice neurogenesis and learning abilities, further experiments on neurons and in vivo studies could be helpful to clarify whether the inhibition of poly(ADP-ribosyl) ation contributes to organic Hg induced neurotoxicity.},
  language  = {en}
}