@misc{BetaBodenschatz2011,
  author    = {Beta, Carsten and Bodenschatz, Eberhard},
  title     = {Microfluidic tools for quantitative studies of eukaryotic chemotaxis},
  series = {European journal of cell biology},
  volume    = {90},
  journal   = {European journal of cell biology},
  number    = {10},
  publisher = {Elsevier},
  address   = {Jena},
  issn      = {0171-9335},
  doi       = {10.1016/j.ejcb.2011.05.006},
  pages     = {811 -- 816},
  year      = {2011},
  abstract  = {Over the past decade, microfluidic techniques have been established as a versatile platform to perform live cell experiments under well-controlled conditions. To investigate the directional responses of cells, stable concentration profiles of chemotactic factors can be generated in microfluidic gradient mixers that provide a high degree of spatial control. However, the times for built-up and switching of gradient profiles are in general too slow to resolve the intracellular protein translocation events of directional sensing of eukaryotes. Here, we review an example of a conventional microfluidic gradient mixer as well as the novel flow photolysis technique that achieves an increased temporal resolution by combining the photo-activation of caged compounds with the advantages of microfluidic chambers.},
  language  = {en}
}