@article{StoeckleinRohdeScharteetal.2000, author = {St{\"o}cklein, Walter F. M. and Rohde, M. and Scharte, Gudrun and Behrsing, Olaf and Warsinke, Axel and Micheel, Burkhard and Scheller, Frieder W.}, title = {Sensitive detection of triazine and phenylurea pesticides in pure organic solvent by enzyme linked immunsorbent assay (ELISA): stabilities, solubilities and sensitives}, year = {2000}, language = {en} } @article{HuangWarsinkeKoroljovaSkorobogatkoetal.1999, author = {Huang, T. and Warsinke, Axel and Koroljova-Skorobogatko, O. V. and Makower, Alexander and Kuwana, T. and Scheller, Frieder W.}, title = {A bienzyme carbon paste electrode for the sensitive detection of NADPH and the measurement of glucose-6- phosphate dehydrogenase}, year = {1999}, language = {en} } @article{GajovicHabermuellerWarsinkeetal.1999, author = {Gajovic, Nenad and Haberm{\"u}ller, K. and Warsinke, Axel and Schuhmann, W. and Scheller, Frieder W.}, title = {A pyruvate oxidase electrode based on an electrochemically deposited redox polymer}, year = {1999}, language = {en} } @article{GajovicWarsinkeHuangetal.1999, author = {Gajovic, Nenad and Warsinke, Axel and Huang, T. and Schulmeister, Thomas and Scheller, Frieder W.}, title = {Characterization and mathematical modelling of a novel bienzyme electrode for L-malate with cofactor recycling}, year = {1999}, language = {en} } @article{RohdeSchenkHeymannetal.1998, author = {Rohde, M. and Schenk, J{\"o}rg A. and Heymann, Stephan and Behrsing, Olaf and Scharte, Gudrun and Kempter, Gerhard and Woller, Jochen and H{\"o}hne, Wolfgang and Warsinke, Axel and Micheel, Burkhard}, title = {Production and characterization of monoclonal antibodeis against urea derivatives}, year = {1998}, language = {en} } @article{SellrieWarsinkeMicheel2008, author = {Sellrie, Frank and Warsinke, Axel and Micheel, Burkhard}, title = {Homogeneous indirect fluorescence quenching immunoassay for the determination of low molecular weight substances}, series = {Analytical \& bioanalytical chemistry}, volume = {386}, journal = {Analytical \& bioanalytical chemistry}, number = {2}, pages = {206 -- 210}, year = {2008}, abstract = {This paper describes the principle of a homogeneous indirect fluorescence quenching immunoassay that uses monoclonal antibodies. It is a carrier-free assay system that is performed completely in solution. The assay system was established for the determination of a low molecular weight substance (hapten), the herbicide diuron, used as a model analyte. A fluorescein-monuron conjugate together with a fluorescence-quenching monoclonal anti-fluorescein antibody and an anti-analyte antibody (here an anti-diuron/monuron monoclonal antibody) were used as central components of the assay. The fluorescein-monuron conjugate can be bound either by the anti-fluorescein monoclonal antibody or by the anti-diuron/ monuron monoclonal antibody. Due to steric hindrance, binding of both antibodies to the conjugate was not possible at the same time. By selecting the antibody concentrations appropriately, a dynamic equilibrium can be established that permits the preferential binding of the anti-diuron/monuron antibody to the conjugate, which allows the fluorescein in the conjugate to fluoresce. This equilibrium can be easily altered by adding free analyte (diuron), which competes with the conjugate to bind to the anti-diuron/monuron antibody. A reduction of anti-diuron/monuron antibody binding to the conjugate results in an increase in the binding of the anti-fluorescein antibody, which leads to a decrease in the fluorescence of the conjugate. The fluorescence is therefore a direct indicator of the state of equilibrium of the system and thus also the presence of free unconjugated analyte. The determination of an analyte based on this test principle does not require any washing steps. After the test components are mixed, the dynamic equilibrium is rapidly reached and the results can be obtained in less than 5 min by measuring the fluorescence of the fluorescein. We used this test principle for the determination of diuron, which was demonstrated for concentrations of approximately 5 nM.}, language = {en} }