@article{SchloerHirschbergBenAmoretal.2022,
  author    = {Schl{\"o}r, Anja and Hirschberg, Stefan and Ben Amor, Ghada and Meister, Toni Luise and Arora, Prerna and P{\"o}hlmann, Stefan and Hoffmann, Markus and Pf{\"a}nder, Stephanie and Eddin, Omar Kamal and Kamhieh-Milz, Julian and Hanack, Katja},
  title     = {SARS-CoV-2 neutralizing camelid heavy-chain-only antibodies as powerful tools for diagnostic and therapeutic applications},
  series = {Frontiers in Immunology},
  journal   = {Frontiers in Immunology},
  publisher = {Frontiers Media SA},
  address   = {Lausanne, Schweiz},
  issn      = {1664-3224},
  doi       = {10.3389/fimmu.2022.930975},
  pages     = {1 -- 14},
  year      = {2022},
  abstract  = {Introduction: The ongoing COVID-19 pandemic situation caused by SARS-CoV-2 and variants of concern such as B.1.617.2 (Delta) and recently, B.1.1.529 (Omicron) is posing multiple challenges to humanity. The rapid evolution of the virus requires adaptation of diagnostic and therapeutic applications. Objectives: In this study, we describe camelid heavy-chain-only antibodies (hcAb) as useful tools for novel in vitro diagnostic assays and for therapeutic applications due to their neutralizing capacity. Methods: Five antibody candidates were selected out of a na{\"i}ve camelid library by phage display and expressed as full length IgG2 antibodies. The antibodies were characterized by Western blot, enzyme-linked immunosorbent assays, surface plasmon resonance with regard to their specificity to the recombinant SARS-CoV-2 Spike protein and to SARS-CoV-2 virus-like particles. Neutralization assays were performed with authentic SARS-CoV-2 and pseudotyped viruses (wildtype and Omicron). Results: All antibodies efficiently detect recombinant SARS-CoV-2 Spike protein and SARS-CoV-2 virus-like particles in different ELISA setups. The best combination was shown with hcAb B10 as catcher antibody and HRP-conjugated hcAb A7.2 as the detection antibody. Further, four out of five antibodies potently neutralized authentic wildtype SARS-CoV-2 and particles pseudotyped with the SARS-CoV-2 Spike proteins of the wildtype and Omicron variant, sublineage BA.1 at concentrations between 0.1 and 0.35 ng/mL (ND50). Conclusion: Collectively, we report novel camelid hcAbs suitable for diagnostics and potential therapy.},
  language  = {en}
}
@misc{SchloerHirschbergBenAmoretal.2022,
  author    = {Schl{\"o}r, Anja and Hirschberg, Stefan and Ben Amor, Ghada and Meister, Toni Luise and Arora, Prerna and P{\"o}hlmann, Stefan and Hoffmann, Markus and Pf{\"a}nder, Stephanie and Eddin, Omar Kamal and Kamhieh-Milz, Julian and Hanack, Katja},
  title     = {SARS-CoV-2 neutralizing camelid heavy-chain-only antibodies as powerful tools for diagnostic and therapeutic applications},
  series = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  journal   = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  number    = {1280},
  issn      = {1866-8372},
  doi       = {10.25932/publishup-57012},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-570124},
  pages     = {14},
  year      = {2022},
  abstract  = {Introduction: The ongoing COVID-19 pandemic situation caused by SARS-CoV-2 and variants of concern such as B.1.617.2 (Delta) and recently, B.1.1.529 (Omicron) is posing multiple challenges to humanity. The rapid evolution of the virus requires adaptation of diagnostic and therapeutic applications. Objectives: In this study, we describe camelid heavy-chain-only antibodies (hcAb) as useful tools for novel in vitro diagnostic assays and for therapeutic applications due to their neutralizing capacity. Methods: Five antibody candidates were selected out of a na{\"i}ve camelid library by phage display and expressed as full length IgG2 antibodies. The antibodies were characterized by Western blot, enzyme-linked immunosorbent assays, surface plasmon resonance with regard to their specificity to the recombinant SARS-CoV-2 Spike protein and to SARS-CoV-2 virus-like particles. Neutralization assays were performed with authentic SARS-CoV-2 and pseudotyped viruses (wildtype and Omicron). Results: All antibodies efficiently detect recombinant SARS-CoV-2 Spike protein and SARS-CoV-2 virus-like particles in different ELISA setups. The best combination was shown with hcAb B10 as catcher antibody and HRP-conjugated hcAb A7.2 as the detection antibody. Further, four out of five antibodies potently neutralized authentic wildtype SARS-CoV-2 and particles pseudotyped with the SARS-CoV-2 Spike proteins of the wildtype and Omicron variant, sublineage BA.1 at concentrations between 0.1 and 0.35 ng/mL (ND50). Conclusion: Collectively, we report novel camelid hcAbs suitable for diagnostics and potential therapy.},
  language  = {en}
}
@misc{WilsonHuhtamiesHucketal.2001,
  author    = {Wilson, Peter and Huhtamies, Mikko and Huck, Stephan and Gahlen, Gundula and Herrmann, Michael and Reimer, Torsten F. and Winnige, Norbert and P{\"o}hlmann, Markus and Pauser, Josef and Fuchs, Antje and M{\"o}bius, Sascha and Heil, Wolfgang and Jacobs, Rainer and Kaiser, Michael and Kroll, Stefan and Blank, Ralf and Gr{\"u}ndel, Olaf and Schl{\"u}rmann, Jan and Geffarth, Renko and Bavendamm, Gundula},
  title     = {Milit{\"a}r und Gesellschaft in der Fr{\"u}hen Neuzeit},
  volume    = {5},
  number    = {2},
  organization    = {Arbeitskreis Milit{\"a}r und Gesellschaft in der Fr{\"u}hen Neuzeit e.V. (Hrsg.)},
  issn      = {1617-9722},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-28126},
  year      = {2001},
  abstract  = {Aus dem Inhalt dieser Ausgabe: BEITR{\"A}GE: Peter H. Wilson: British and american perspectives on early modern warfare Mikko Huhtamies: Kriegswesen und Gesellschaft in der fr{\"u}hen Neuzeit in der finnisch-schwedischen Geschichtsforschung INTERNET: Stephan Huck: Nutzungsm{\"o}glichkeiten des Internets Gundula Gahlen , Michael Herrmann, Torsten F. Reimer und Norbert Winnige: Milit{\"a}rgeschichte der Fr{\"u}hen Neuzeit im Internet Die Online-Angebote des AMG und sfn Markus P{\"o}hlmann: Die Internetpr{\"a}senz des Arbeitskreises Milit{\"a}rgeschichte e.V. Josef Pauser: Das Online-Angebot des Arbeitskreises .Policey/Polizei im vormodernen Europa. Torsten F. Reimer: Eine Studie in Kriegf{\"u}hrung. Eine Vorstellung des H-War Military History Network PROJEKTE: Antje Fuchs: Ein neuer Konfessionskrieg? Erfahrungen von Krieg und Okkupation im Kurf{\"u}rstentum Hannover und benachbarten geistlichen F{\"u}rstent{\"u}mern zur Zeit des Siebenj{\"a}hrigen Krieges 1756-1763 Sascha M{\"o}bius: Ergebnisse der Magisterarbeit .Psychologische Aspekte friederizianischer Taktik im Siebenj{\"a}hrigen Krieg. Wolfgang Heil: Die Gemeinen Soldaten. Das Sozialleben der milit{\"a}rischen Unterschicht im altpreußischen Heer und seine Stellung in der altst{\"a}ndischen Gesellschaft. Rainer Jacobs: Milit{\"a}rische Dienstpflichten in der Fr{\"u}hen Neuzeit Michael Kaiser und Stefan Kroll: Forschungsprojekt Milit{\"a}r und Religiosit{\"a}t in der Fr{\"u}hen Neuzeit Ralf Blank: Die Schlosskanonen von Hohenlimburg Artilleriegesch{\"u}tze mit einer wechselvollen Geschichte Gundula Gahlen und Olaf Gr{\"u}ndel: Kataster zur Schlacht bei Wittstock von 1636 Jan Schl{\"u}rmann: Die Schleswig-Holstein-Gottorfischen Auxiliairtruppen im Spanischen Erbfolgekrieg . Ergebnisse einer Untersuchung BERICHTE: Renko Geffarth: Die besetzte res publica. Zum Verh{\"a}ltnis von ziviler Obrigkeit und milit{\"a}rischer Herrschaft in besetzten Gebieten vom Sp{\"a}tmittelalter bis zum 18. Jahrhundert. Gundula Bavendamm: Operationsgeschichte und moderne Historiographie. Ein Widerspruch?},
  subject      = {Milit{\"a}r / Geschichte},
  language  = {de}
}