@article{SassStoeckleinKlevesathetal.2019,
  author    = {Sass, Stephan and St{\"o}cklein, Walter F. M. and Klevesath, Anja and Hurpin, Jeanne and Menger, Marcus and Hille, Carsten},
  title     = {Binding affinity data of DNA aptamers for therapeutic anthracyclines from microscale thermophoresis and surface plasmon resonance spectroscopy},
  series = {The analyst : the analytical journal of the Royal Society of Chemistry},
  volume    = {144},
  journal   = {The analyst : the analytical journal of the Royal Society of Chemistry},
  number    = {20},
  publisher = {Royal Society of Chemistry},
  address   = {Cambridge},
  issn      = {0003-2654},
  doi       = {10.1039/c9an01247h},
  pages     = {6064 -- 6073},
  year      = {2019},
  abstract  = {Anthracyclines like daunorubicin (DRN) and doxorubicin (DOX) play an undisputed key role in cancer treatment, but their chronic administration can cause severe side effects. For precise anthracycline analytical systems, aptamers are preferable recognition elements. Here, we describe the detailed characterisation of a single-stranded DNA aptamer DRN-10 and its truncated versions for DOX and DRN detection. Binding affinities were determined from surface plasmon resonance (SPR) and microscale thermophoresis (MST) and combined with conformational data from circular dichroism (CD). Both aptamers displayed similar nanomolar binding affinities to DRN and DOX, even though their rate constants differed as shown by SPR recordings. SPR kinetic data unravelled a two-state reaction model including a 1 : 1 binding and a subsequent conformational change of the binding complex. This model was supported by CD spectra. In addition, the dissociation constants determined with MST were always lower than that from SPR, and especially for the truncated aptamer they differed by two orders of magnitude. This most probably reflects the methodological difference, namely labelling for MST vs. immobilisation for SPR. From CD recordings, we suggested a specific G-quadruplex as structural basis for anthracycline binding. We concluded that the aptamer DRN-10 is a promising recognition element for anthracycline detection systems and further selected aptamers can be also characterised with the combined methodological approach presented here.},
  language  = {en}
}
@article{ZuehlkeSassRiebeetal.2017,
  author    = {Z{\"u}hlke, Martin and Sass, Stephan and Riebe, Daniel and Beitz, Toralf and L{\"o}hmannsr{\"o}ben, Hans-Gerd},
  title     = {Real-Time Reaction Monitoring of an Organic Multistep Reaction by Electrospray Ionization-Ion Mobility Spectrometry},
  series = {ChemPlusChem},
  volume    = {82},
  journal   = {ChemPlusChem},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {2192-6506},
  doi       = {10.1002/cplu.201700296},
  pages     = {1266 -- 1273},
  year      = {2017},
  abstract  = {The capability of electrospray ionization (ESI)-ion mobility (IM) spectrometry for reaction monitoring is assessed both as a stand-alone real-time technique and in combination with HPLC. A three-step chemical reaction, consisting of a Williamson ether synthesis followed by a hydrogenation and an N-alkylation step, is chosen for demonstration. Intermediates and products are determined with a drift time to mass-per-charge correlation. Addition of an HPLC column to the setup increases the separation power and allows the determination of further species. Monitoring of the intensities of the various species over the reaction time allows the detection of the end of reaction, determination of the rate-limiting step, observation of the system response in discontinuous processes, and optimization of the mass ratios of the starting materials. However, charge competition in ESI influences the quantitative detection of substances in the reaction mixture. Therefore, two different methods are investigated, which allow the quantification and investigation of reaction kinetics. The first method is based on the pre-separation of the compounds on an HPLC column and their subsequent individual detection in the ESI-IM spectrometer. The second method involves an extended calibration procedure, which considers charge competition effects and facilitates nearly real-time quantification.},
  language  = {en}
}
@article{LauKubiakBurchertetal.2014,
  author    = {Lau, Stephan and Kubiak, Thomas and Burchert, Sebastian and Goering, Mark and Oberlaender, Nils and von Mauschwitz, Hannes and von Sass, Sarah and Selle, Mareen and Hiemisch, Anette},
  title     = {Disentangling the effects of optimism and attributions on feelings of success},
  series = {Personality and individual differences : an international journal of research into the structure and development of personality, and the causation of individual differences},
  volume    = {56},
  journal   = {Personality and individual differences : an international journal of research into the structure and development of personality, and the causation of individual differences},
  publisher = {Elsevier},
  address   = {Oxford},
  issn      = {0191-8869},
  doi       = {10.1016/j.paid.2013.08.030},
  pages     = {78 -- 82},
  year      = {2014},
  abstract  = {Two experiments examined the effects of dispositional optimism and attributions on feelings of success in a performance setting. In Experiment 1, participants successfully solved three cognitive tasks and attributed the success either internally (i.e., to themselves) or externally (i.e., to a teammate). We found no effect of optimism, but a significant effect of the attribution: Internal attribution predicted an increase in feelings of success. In Experiment 2, we replicated the design and adopted an extreme groups approach in order to include the extremes of the optimism dimension. Only optimism affected feelings of success in this sample: Pessimistic participants showed higher increases in feelings of success than optimistic participants. We conclude that optimism, if disentangled from attribution, may have an effect on affect, with pessimism showing potential affective benefits. However, this association may be concealed if samples with a restricted range of the optimism dimension are studied.},
  language  = {en}
}
@article{RuehlSassNauetal.2001,
  author    = {R{\"u}hl, Ralph and Sass, J{\"o}rn Oliver and Nau, Heinz and Klug, Stephan},
  title     = {Effects of all-trans-retinoic acid and all-trans-retinoyl glucuronide in two in vitro systems of distinct biological complexity},
  year      = {2001},
  language  = {en}
}
@article{OnalSassHurpinetal.2017,
  author    = {Onal, Emel and Sass, Stephan and Hurpin, Jeanne and Ertekin, Kadriye and Topal, Sevinc Zehra and Kumke, Michael Uwe and Hirel, Catherine},
  title     = {Lifetime-Based Oxygen Sensing Properties of palladium(II) and platinum(II) meso-tetrakis(4-phenylethynyl)phenylporphyrin},
  series = {Journal of fluorescence},
  volume    = {27},
  journal   = {Journal of fluorescence},
  publisher = {Springer},
  address   = {New York},
  issn      = {1053-0509},
  doi       = {10.1007/s10895-016-2022-x},
  pages     = {861 -- 868},
  year      = {2017},
  abstract  = {High oxygen permeable [poly(TMSP)] nanofibers incorporating porphyrin macrocycle as luminescence indicators were prepared by electrospinning technique. The porphyrins involves were modified by i) introducing phenylacetylide substituents on the para position of the phenyl moieties and ii) varying the metal centers [Pt(II) or Pd(II)] of the meso-tetrakisphenylporphyrins. A set of nanofibers; (Pt-TPP)NF, (Pd-TPP)NF, (Pt-TPA)NF and (Pd-TPA)NF were obtained to study their structure-activity relationship toward oxygen. The lifetime-based technique was privileged to take advantage of their long-lived phosphorescent properties. A two-fold enhancement was observed for (Pt-TPA)NF and (Pd-TPA)NF compared to (Pt-TPP)NF and (Pd-TPP)NF demonstrating the positive effect of the phenylacetylide moieties on the lifetime. Also, Silver nanoparticles were included in nanofibers to investigate their influence on lifetime-based oxygen sensitivity, showing that the presence of AgNPs only affects (Pd-TPA)NF.},
  language  = {en}
}
@phdthesis{Sass2020,
  author    = {Saß, Stephan},
  title     = {Optische chemische Sensorik mittels Phasenmodulationsspektroskopie},
  school      = {Universit{\"a}t Potsdam},
  pages     = {102, xviii},
  year      = {2020},
  abstract  = {Die vorgelegte Arbeit besteht aus drei Teilprojekten, der Realisierung eines Multiparametersensors (Temperatur, pH-Wert und Sauerstoffkonzentration), der Konzipierung und Untersuchung eines optischen Atemgassensors und Untersuchungen zur Anwendung des Konzeptes der Sauerstoffl{\"o}schung in der Immuntechnologie. Zur Realisierung des Multiparametersensors wurden die einzelnen Sensorfarbstoffe, sofern notwendig, synthetisiert und anschließend einzeln unter Laborbedingungen charakterisiert. Im weiteren Verlauf wurde ein Versuchsaufbau konzipiert mit dem es m{\"o}glich ist, alle verwendeten Sensorfarbstoffe mit einer Anregungsquelle anzuregen. Dabei erfolgte die Detektion der Parameter Temperatur und Sauerstoffkonzentration mittels Phasenmodulationsspektroskopie und die pH-Wert-bestimmung mittels station{\"a}rer Fluoreszenzspektroskopie. So konnte ein Multiparametersensor konzipiert werden, mit dem es m{\"o}glich ist, die drei genannten Parameter simultan, in Echtzeit und ohne externe Temperaturmessung zu detektieren. Im Rahmen der Entwicklung eines optischen Atemgassensors konnte zun{\"a}chst eine neue Sensorform entwickelt werden. Durch diese neue Sensorform, welche sich durch sehr kurze Ansprechzeiten auszeichnet, ist es m{\"o}glich den Sauerstoffgehalt in der Exspirationsluft sehr detailreich zu erfassen. Durch freiwillige Selbstversuche mit dem Atemgassensor konnte eine Korrelation mit einer etablierten Untersuchungsmethode hergestellt werden. W{\"a}hrend der Untersuchungen zur Anwendung des Konzeptes der Sauerstoffl{\"o}schung in der Immuntechnologie konnte zun{\"a}chst ein Modell entwickelt werden, welches die Wechselwirkung zwischen Antik{\"o}rper und synthetisiertem Farbstoff, welcher als Antigen fungierte, beschreibt. Nachdem weiterhin eine Wechselwirkung zwischen Antik{\"o}rper und Antigen in einfachen Medien, wie PBS-Pufferl{\"o}sung, gezeigt werden konnte, gelang dies auch in komplexen Medien wie bovinem Serum, Kuhmilch oder Speichelfl{\"u}ssigkeit. So konnte ein System entwickelt werden, mit dem es m{\"o}glich ist Antik{\"o}rper-Antigen-Wechselwirkungen in komplexen biologischen Medien zu verfolgen.},
  language  = {de}
}