@article{WolffSchuelerGastetal.2020, author = {Wolff, Martin and Sch{\"u}ler, Anja and Gast, Klaus and Seckler, Robert and Evers, Andreas and Pfeiffer-Marek, Stefania and Kurz, Michael and Nagel, Norbert and Haack, Torsten and Wagner, Michael and Thalhammer, Anja}, title = {Self-Assembly of Exendin-4-Derived Dual Peptide Agonists is Mediated by Acylation and Correlated to the Length of Conjugated Fatty Acyl Chains}, series = {Molecular pharmaceutics}, volume = {17}, journal = {Molecular pharmaceutics}, number = {3}, publisher = {American Chemical Society}, address = {Washington}, issn = {1543-8384}, doi = {10.1021/acs.molpharmaceut.9b01195}, pages = {965 -- 978}, year = {2020}, abstract = {Dual glucagon-like peptide-1/glucagon receptor agonists have emerged as promising candidates for the treatment of diabetes and obesity. Issues of degradation sensitivity and rapid renal clearance are addressed, for example, by the conjugation of peptides to fatty acid chains, promoting reversible albumin binding. We use combined dynamic and static light scattering to directly measure the self-assembly of a set of dual peptide agonists based on the exendin-4 structure with varying fatty acid chain lengths in terms of apparent molecular mass and hydrodynamic radius (R-S). We use NMR spectroscopy to gain an insight into the molecular architecture of the assembly. We investigate conformational changes of the monomeric subunits resulting from peptide self-assembly and assembly stability as a function of the fatty acid chain length using circular dichroism and fluorescence spectroscopy. Our results demonstrate that self-assembly of the exendin-4-derived dual agonist peptides is essentially driven by hydrophobic interactions involving the conjugated acyl chains. The fatty acid chain length affects assembly equilibria and the assembly stability, although the peptide subunits in the assembly retain a dynamic secondary structure. The assembly architecture is characterized by juxtaposition of the fatty acyl side chains and a hydrophobic cluster of the peptide moiety. This cluster experiences local conformational changes in the assembly compared to the monomeric unit leading to a reduction in solvent exposure. The N-terminal half of the peptide and a C-terminal loop are not in contact with neighboring peptide subunits in the assemblies. Altogether, our study contributes to a thorough understanding of the association characteristics and the tendency toward self-assembly in response to lipidation. This is important not only to achieve the desired bioavailability but also with respect to the physical stability of peptide solutions.}, language = {en} } @article{WolffSchuelerGastetal.2020, author = {Wolff, Martin and Sch{\"u}ler, Anja and Gast, Klaus and Seckler, Robert and Evers, Andreas and Pfeiffer-Marek, Stefania and Kurz, Michael and Nagel, Norbert and Haack, Torsten and Wagner, Michael and Thalhammer, Anja}, title = {Self-Assembly of Exendin-4-Derived Dual Peptide Agonists is Mediated by Acylation and Correlated to the Length of Conjugated Fatty Acyl Chains}, series = {Molecular pharmaceutics}, volume = {17}, journal = {Molecular pharmaceutics}, number = {3}, publisher = {American Chemical Society}, address = {Washington}, issn = {1543-8384}, doi = {10.1021/acs.molpharmaceut.9b01195}, pages = {965 -- 978}, year = {2020}, abstract = {Dual glucagon-like peptide-1/glucagon receptor agonists have emerged as promising candidates for the treatment of diabetes and obesity. Issues of degradation sensitivity and rapid renal clearance are addressed, for example, by the conjugation of peptides to fatty acid chains, promoting reversible albumin binding. We use combined dynamic and static light scattering to directly measure the self-assembly of a set of dual peptide agonists based on the exendin-4 structure with varying fatty acid chain lengths in terms of apparent molecular mass and hydrodynamic radius (R-S). We use NMR spectroscopy to gain an insight into the molecular architecture of the assembly. We investigate conformational changes of the monomeric subunits resulting from peptide self-assembly and assembly stability as a function of the fatty acid chain length using circular dichroism and fluorescence spectroscopy. Our results demonstrate that self-assembly of the exendin-4-derived dual agonist peptides is essentially driven by hydrophobic interactions involving the conjugated acyl chains. The fatty acid chain length affects assembly equilibria and the assembly stability, although the peptide subunits in the assembly retain a dynamic secondary structure. The assembly architecture is characterized by juxtaposition of the fatty acyl side chains and a hydrophobic cluster of the peptide moiety. This cluster experiences local conformational changes in the assembly compared to the monomeric unit leading to a reduction in solvent exposure. The N-terminal half of the peptide and a C-terminal loop are not in contact with neighboring peptide subunits in the assemblies. Altogether, our study contributes to a thorough understanding of the association characteristics and the tendency toward self-assembly in response to lipidation. This is important not only to achieve the desired bioavailability but also with respect to the physical stability of peptide solutions.}, language = {en} } @article{WillmannHeniLinderetal.2019, author = {Willmann, Caroline and Heni, Martin and Linder, Katarzyna and Wagner, Robert and Stefan, Norbert and Machann, J{\"u}rgen and Schulze, Matthias Bernd and Joost, Hans-Georg and Haring, Hans-Ulrich and Fritsche, Andreas}, title = {Potential effects of reduced red meat compared with increased fiber intake on glucose metabolism and liver fat content}, series = {The American journal of clinical nutrition : a publication of the American Society for Nutrition, Inc.}, volume = {109}, journal = {The American journal of clinical nutrition : a publication of the American Society for Nutrition, Inc.}, number = {2}, publisher = {Oxford Univ. Press}, address = {Oxford}, issn = {0002-9165}, doi = {10.1093/ajcn/nqy307}, pages = {288 -- 296}, year = {2019}, abstract = {Background: Epidemiological studies suggest that an increased red meat intake is associated with a higher risk of type 2 diabetes, whereas an increased fiber intake is associated with a lower risk. Objectives: We conducted an intervention study to investigate the effects of these nutritional factors on glucose and lipid metabolism, body-fat distribution, and liver fat content in subjects at increased risk of type 2 diabetes. Methods: This prospective, randomized, and controlled dietary intervention study was performed over 6 mo. All groups decreased their daily caloric intake by 400 kcal. The "control" group (N = 40) only had this requirement. The "no red meat" group (N = 48) in addition aimed to avoid the intake of red meat, and the "fiber" group (N = 44) increased intake of fibers to 40 g/d. Anthropometric parameters and frequently sampled oral glucose tolerance tests were performed before and after intervention. Body-fat mass and distribution, liver fat, and liver iron content were assessed by MRI and single voxel proton magnetic resonance spectroscopy. Results: Participants in all groups lost weight (mean 3.3 +/- 0.5 kg, P < 0.0001). Glucose tolerance and insulin sensitivity improved (P < 0.001), and body and visceral fat mass decreased in all groups (P < 0.001). These changes did not differ between groups. Liver fat content decreased significantly (P < 0.001) with no differences between the groups. The decrease in liver fat correlated with the decrease in ferritin during intervention (r(2) = 0.08, P = 0.0021). This association was confirmed in an independent lifestyle intervention study (Tuebingen Lifestyle Intervention Program, N = 229, P = 0.0084). Conclusions: Our data indicate that caloric restriction leads to a marked improvement in glucose metabolism and body-fat composition, including liver-fat content. The marked reduction in liver fat might be mediated via changes in ferritin levels. In the context of caloric restriction, there seems to be no additional beneficial impact of reduced red meat intake and increased fiber intake on the improvement in cardiometabolic risk parameters. This trial was registered at clinicaltrials.gov as NCT03231839.}, language = {en} } @article{Wagner2018, author = {Wagner, Robert}, title = {Schulpraktisches Klavierspiel und inklusives Musizieren im schulischen Kontext}, series = {Potsdamer Schriftenreihe zur Musikp{\"a}dagogik}, journal = {Potsdamer Schriftenreihe zur Musikp{\"a}dagogik}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-417674}, pages = {139 -- 151}, year = {2018}, language = {de} } @book{SchmidtWagnerSchwerdtfegeretal.2022, author = {Schmidt, Thorsten Ingo and Wagner, Dieter and Schwerdtfeger, Roswitha and Sch{\"a}fer, Andrea and Musil, Andreas and Edeling, Thomas and Bauer, Hartmut and Kinyakin, Andrey and Loladze, Besik and Nehls, Danny and Maaß, Christian and Kuhlmann, Sabine and Kuckei, Daniel A. and Hein, Victoria and Wille, Robert and Franzke, Jochen and B{\"u}chner, Christiane}, title = {Festschrift f{\"u}r Dr. Christiane B{\"u}chner in W{\"u}rdigung ihres Wirkens am Kommunalwissenschaftlichen Institut (1994-2022)}, series = {KWI Schriften}, journal = {KWI Schriften}, number = {13}, editor = {Franzke, Jochen}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-529-3}, issn = {1867-951X}, doi = {10.25932/publishup-54498}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-544987}, publisher = {Universit{\"a}t Potsdam}, pages = {125}, year = {2022}, abstract = {Diese eher ungew{\"o}hnliche, aber sehr pers{\"o}nlich gehaltene Festschrift ist dem lang­j{\"a}hrigen Wirken von Dr. Christiane B{\"u}chner als „Gesch{\"a}ftsf{\"u}hrerin" am Kommunal­wissenschaftlichen Institut (KWI) der Universit{\"a}t Potsdam gewidmet. Die von Prof. Jochen Franzke zusammengestellte und herausgegebene Publikation enth{\"a}lt im ersten Teil neben dem Grußwort des Gesch{\"a}ftsf{\"u}hrenden Direktors des KWI Herrn Prof. Thorsten Ingo Schmidt eine Reihe pers{\"o}nlicher W{\"u}rdigungen von Kolleginnen und Kollegen, Gastwissenschaftlern und Mitarbeitenden, die seit 1994 in verschiedenen Phasen der Entwicklung des KWIs mit Dr. Christiane B{\"u}chner eng zusammengearbeitet haben. Der abschließende Dokumentationsteil der Publikation enth{\"a}lt neben Ausz{\"u}gen aus dem Schriftenverzeichnis von Dr. Christiane B{\"u}chner auch zwei Nachdrucke aus deren Feder zum Thema der Kreisgebietsreform in Brandenburg (von 2001) sowie {\"u}ber den Landkreis Barnim (von 2019).}, language = {de} } @misc{RianoPachonNagelNeigenfindetal.2009, author = {Riano-Pachon, Diego Mauricio and Nagel, Axel and Neigenfind, Jost and Wagner, Robert and Basekow, Rico and Weber, Elke and M{\"u}ller-R{\"o}ber, Bernd and Diehl, Svenja and Kersten, Birgit}, title = {GabiPD : the GABI primary database - a plant integrative "omics" database}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-45075}, year = {2009}, abstract = {The GABI Primary Database, GabiPD (http:// www.gabipd.org/), was established in the frame of the German initiative for Genome Analysis of the Plant Biological System (GABI). The goal of GabiPD is to collect, integrate, analyze and visualize primary information from GABI projects. GabiPD constitutes a repository and analysis platform for a wide array of heterogeneous data from high-throughput experiments in several plant species. Data from different 'omics' fronts are incorporated (i.e. genomics, transcriptomics, proteomics and metabolomics), originating from 14 different model or crop species. We have developed the concept of GreenCards for textbased retrieval of all data types in GabiPD (e.g. clones, genes, mutant lines). All data types point to a central Gene GreenCard, where gene information is integrated from genome projects or NCBI UniGene sets. The centralized Gene GreenCard allows visualizing ESTs aligned to annotated transcripts as well as displaying identified protein domains and gene structure. Moreover, GabiPD makes available interactive genetic maps from potato and barley, and protein 2DE gels from Arabidopsis thaliana and Brassica napus. Gene expression and metabolic-profiling data can be visualized through MapManWeb. By the integration of complex data in a framework of existing knowledge, GabiPD provides new insights and allows for new interpretations of the data.}, language = {en} } @book{MeinelThomasWarschofskyetal.2011, author = {Meinel, Christoph and Thomas, Ivonne and Warschofsky, Robert and Menzel, Michael and Junker, Holger and Schwenk, J{\"o}rg and Roth, Volker and Peters, Jan and Raepple, Martin and St{\"o}rtkuhl, Thomas and Quint, Bruno and Kleinhenz, Michael and Wagner, Daniel}, title = {SOA Security 2010 : Symposium f{\"u}r Sicherheit in Service-orientierten Architekturen ; 28. / 29. Oktober 2010 am Hasso-Plattner-Institut}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-110-3}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-49696}, publisher = {Universit{\"a}t Potsdam}, pages = {80}, year = {2011}, abstract = {"Forschung meets Business" - diese Kombination hat in den vergangenen Jahren immer wieder zu zahlreichen interessanten und fruchtbaren Diskussionen gef{\"u}hrt. Mit dem Symposium "Sicherheit in Service-orientierten Architekturen" f{\"u}hrt das Hasso-Plattner-Institut diese Tradition fort und lud alle Interessenten zu einem zweit{\"a}gigen Symposium nach Potsdam ein, um gemeinsam mit Fachvertretern aus der Forschung und Industrie {\"u}ber die aktuellen Entwicklungen im Bereich Sicherheit von SOA zu diskutieren. Die im Rahmen dieses Symposiums vorgestellten Beitr{\"a}ge fokussieren sich auf die Sicherheitsthemen "Sichere Digitale Identit{\"a}ten und Identit{\"a}tsmanagement", "Trust Management", "Modell-getriebene SOA-Sicherheit", "Datenschutz und Privatsph{\"a}re", "Sichere Enterprise SOA", und "Sichere IT-Infrastrukturen".}, language = {de} } @misc{LiuKaempfBussertetal.2018, author = {Liu, Qi and K{\"a}mpf, Horst and Bussert, Robert and Krauze, Patryk and Horn, Fabian and Nickschick, Tobias and Plessen, Birgit and Wagner, Dirk and Alawi, Mashal}, title = {Influence of CO2 degassing on the microbial community in a dry mofette field in Hartoušov, Czech Republic (Western Eger Rift)}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, number = {1100}, issn = {1866-8372}, doi = {10.25932/publishup-47115}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-471153}, pages = {19}, year = {2018}, abstract = {The Cheb Basin (CZ) is a shallow Neogene intracontinental basin filled with fluvial and lacustrine sediments that is located in the western part of the Eger Rift. The basin is situated in a seismically active area and is characterized by diffuse degassing of mantle-derived CO2 in mofette fields. The Hartousov mofette field shows a daily CO2 flux of 23-97 tons of CO2 released over an area of 0.35 km(2) and a soil gas concentration of up to 100\% CO2. The present study aims to explore the geo-bio interactions provoked by the influence of elevated CO2 concentrations on the geochemistry and microbial community of soils and sediments. To sample the strata, two 3-m cores were recovered. One core stems from the center of the degassing structure, whereas the other core was taken 8 m from the ENE and served as an undisturbed reference site. The sites were compared regarding their geochemical features, microbial abundances, and microbial community structures. The mofette site is characterized by a low pH and high TOC/sulfate contents. Striking differences in the microbial community highlight the substantial impact of elevated CO2 concentrations and their associated side effects on microbial processes. The abundance of microbes did not show a typical decrease with depth, indicating that the uprising CO2-rich fluid provides sufficient substrate for chemolithoautotrophic anaerobic microorganisms. Illumine MiSeq sequencing of the 16S rRNA genes and multivariate statistics reveals that the pH strongly influences microbial composition and explains around 38.7\% of the variance at the mofette site and 22.4\% of the variance between the mofette site and the undisturbed reference site. Accordingly, acidophilic microorganisms (e.g., OTUs assigned to Acidobacteriaceae and Acidithiobacillus) displayed a much higher relative abundance at the mofette site than at the reference site. The microbial community at the mofette site is characterized by a high relative abundance of methanogens and taxa involved in sulfur cycling. The present study provides intriguing insights into microbial life and geo-bio interactions in an active seismic region dominated by emanating mantle-derived CO2-rich fluids, and thereby builds the basis for further studies, e.g., focusing on the functional repertoire of the communities. However, it remains open if the observed patterns can be generalized for different time-points or sites.}, language = {en} } @article{LiuKaempfBussertetal.2018, author = {Liu, Qi and K{\"a}mpf, Horst and Bussert, Robert and Krauze, Patryk and Horn, Fabian and Nickschick, Tobias and Plessen, Birgit and Wagner, Dirk and Alawi, Mashal}, title = {Influence of CO2 degassing on the microbial community in a dry mofette field in Hartoušov, Czech Republic (Western Eger Rift)}, series = {Frontiers in Microbiology}, volume = {9}, journal = {Frontiers in Microbiology}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {1664-302X}, doi = {10.3389/fmicb.2018.02787}, pages = {17}, year = {2018}, abstract = {The Cheb Basin (CZ) is a shallow Neogene intracontinental basin filled with fluvial and lacustrine sediments that is located in the western part of the Eger Rift. The basin is situated in a seismically active area and is characterized by diffuse degassing of mantle-derived CO2 in mofette fields. The Hartousov mofette field shows a daily CO2 flux of 23-97 tons of CO2 released over an area of 0.35 km(2) and a soil gas concentration of up to 100\% CO2. The present study aims to explore the geo-bio interactions provoked by the influence of elevated CO2 concentrations on the geochemistry and microbial community of soils and sediments. To sample the strata, two 3-m cores were recovered. One core stems from the center of the degassing structure, whereas the other core was taken 8 m from the ENE and served as an undisturbed reference site. The sites were compared regarding their geochemical features, microbial abundances, and microbial community structures. The mofette site is characterized by a low pH and high TOC/sulfate contents. Striking differences in the microbial community highlight the substantial impact of elevated CO2 concentrations and their associated side effects on microbial processes. The abundance of microbes did not show a typical decrease with depth, indicating that the uprising CO2-rich fluid provides sufficient substrate for chemolithoautotrophic anaerobic microorganisms. Illumine MiSeq sequencing of the 16S rRNA genes and multivariate statistics reveals that the pH strongly influences microbial composition and explains around 38.7\% of the variance at the mofette site and 22.4\% of the variance between the mofette site and the undisturbed reference site. Accordingly, acidophilic microorganisms (e.g., OTUs assigned to Acidobacteriaceae and Acidithiobacillus) displayed a much higher relative abundance at the mofette site than at the reference site. The microbial community at the mofette site is characterized by a high relative abundance of methanogens and taxa involved in sulfur cycling. The present study provides intriguing insights into microbial life and geo-bio interactions in an active seismic region dominated by emanating mantle-derived CO2-rich fluids, and thereby builds the basis for further studies, e.g., focusing on the functional repertoire of the communities. However, it remains open if the observed patterns can be generalized for different time-points or sites.}, language = {en} } @article{LiuAdlerLipusetal.2020, author = {Liu, Qi and Adler, Karsten and Lipus, Daniel and K{\"a}mpf, Horst and Bussert, Robert and Plessen, Birgit and Schulz, Hans-Martin and Krauze, Patryk and Horn, Fabian and Wagner, Dirk and Mangelsdorf, Kai and Alawi, Mashal}, title = {Microbial signatures in deep CO2-saturated miocene sediments of the active Hartousov mofette system (NW Czech Republic)}, series = {Frontiers in microbiology}, volume = {11}, journal = {Frontiers in microbiology}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {1664-302X}, doi = {10.3389/fmicb.2020.543260}, pages = {21}, year = {2020}, abstract = {The Hartousov mofette system is a natural CO2 degassing site in the central Cheb Basin (Eger Rift, Central Europe). In early 2016 a 108 m deep core was obtained from this system to investigate the impact of ascending mantle-derived CO2 on indigenous deep microbial communities and their surrounding life habitat. During drilling, a CO2 blow out occurred at a depth of 78.5 meter below surface (mbs) suggesting a CO2 reservoir associated with a deep low-permeable CO2-saturated saline aquifer at the transition from Early Miocene terrestrial to lacustrine sediments. Past microbial communities were investigated by hopanoids and glycerol dialkyl glycerol tetraethers (GDGTs) reflecting the environmental conditions during the time of deposition rather than showing a signal of the current deep biosphere. The composition and distribution of the deep microbial community potentially stimulated by the upward migration of CO2 starting during Mid Pleistocene time was investigated by intact polar lipids (IPLs), quantitative polymerase chain reaction (qPCR), and deoxyribonucleic acid (DNA) analysis. The deep biosphere is characterized by microorganisms that are linked to the distribution and migration of the ascending CO2-saturated groundwater and the availability of organic matter instead of being linked to single lithological units of the investigated rock profile. Our findings revealed high relative abundances of common soil and water bacteria, in particular the facultative, anaerobic and potential iron-oxidizing Acidovorax and other members of the family Comamonadaceae across the whole recovered core. The results also highlighted the frequent detection of the putative sulfate-oxidizing and CO2-fixating genus Sulfuricurvum at certain depths. A set of new IPLs are suggested to be indicative for microorganisms associated to CO2 accumulation in the mofette system.}, language = {en} }