@article{SchellenbergReichertHardtetal.2020,
  author    = {Schellenberg, Johannes and Reichert, Jessica and Hardt, Martin and Klingelh{\"o}fer, Ines and Morlock, Gertrud and Schubert, Patrick and Bižić, Mina and Grossart, Hans-Peter and K{\"a}mpfer, Peter and Wilke, Thomas and Glaeser, Stefanie P.},
  title     = {The bacterial microbiome of the long-term aquarium cultured high-microbial abundance sponge Haliclona cnidata},
  series = {Frontiers in Marine Science},
  volume    = {7},
  journal   = {Frontiers in Marine Science},
  publisher = {Frontiers Media},
  address   = {Lausanne},
  issn      = {2296-7745},
  doi       = {10.3389/fmars.2020.00266},
  pages     = {20},
  year      = {2020},
  abstract  = {Marine sponges host highly diverse but specific bacterial communities that provide essential functions for the sponge holobiont, including antimicrobial defense. Here, we characterized the bacterial microbiome of the marine sponge Haliclona cnidata that has been in culture in an artificial marine aquarium system. We tested the hypotheses (1) that the long-term aquarium cultured sponge H. cnidata is tightly associated with a typical sponge bacterial microbiota and (2) that the symbiotic Bacteria sustain bioactivity under harmful environmental conditions to facilitate holobiont survival by preventing pathogen invasion. Microscopic and phylogenetic analyses of the bacterial microbiota revealed that H. cnidata represents a high microbial abundance (HMA) sponge with a temporally stable bacterial community that significantly shifts with changing aquarium conditions. A 4-week incubation experiment was performed in small closed aquarium systems with antibiotic and/or light exclusion treatments to reduce the total bacterial and photosynthetically active sponge-associated microbiota to a treatment-specific resilient community. While the holobiont was severely affected by the experimental treatment (i.e., bleaching of the sponge, reduced bacterial abundance, shifted bacterial community composition), the biological defense and bacterial community interactions (i.e., quorum sensing activity) remained intact. 16S rRNA gene amplicon sequencing revealed a resilient community of 105 bacterial taxa, which remained in the treated sponges. These 105 taxa accounted for a relative abundance of 72-83\% of the bacterial sponge microbiota of non-treated sponge fragments that have been cultured under the same conditions. We conclude that a sponge-specific resilient community stays biologically active under harmful environmental conditions, facilitating the resilience of the holobiont. In H. cnidata, bacteria are located in bacteriocytes, which may have contributed to the observed phenomenon.},
  language  = {en}
}
@article{SchubertPreisBlakesleyetal.2013,
  author    = {Schubert, Marcel and Preis, Eduard and Blakesley, James C. and Pingel, Patrick and Scherf, Ullrich and Neher, Dieter},
  title     = {Mobility relaxation and electron trapping in a donor/acceptor copolymer},
  series = {Physical review : B, Condensed matter and materials physics},
  volume    = {87},
  journal   = {Physical review : B, Condensed matter and materials physics},
  number    = {2},
  publisher = {American Physical Society},
  address   = {College Park},
  issn      = {1098-0121},
  doi       = {10.1103/PhysRevB.87.024203},
  pages     = {12},
  year      = {2013},
  abstract  = {To address the nature of charge transport and the origin of severe (intrinsic) trapping in electron-transporting polymers, transient and steady-state charge transport measurements have been conducted on the prototype donor/acceptor copolymer poly[2,7-(9,9-dialkyl-fluorene)-alt-5,5-(4',7'-di-2-thienyl-2',1',3'-benzothiadiazole)] (PFTBTT). A charge-generation layer technique is used to selectively address transport of the desired charge carrier type, to perform time-of-flight measurements on samples with < 200 nm thickness, and to combine the time-of-flight and the photocharge extraction by linearly increasing voltage (photo-CELIV) techniques to investigate charge carrier dynamics over a wide time range. Significant trapping of free electrons is observed in the bulk of dioctyl-substituted PFTBTT (alt-PF8TBTT), introducing a strong relaxation of the charge carrier mobility with time. We used Monte-Carlo simulation to simulate the measured transient data and found that all measurements can be modeled with a single parameter set, with the charge transport behavior determined by multiple trapping and detrapping of electrons in an exponential trap distribution. The influence of the concomitant mobility relaxation on the transient photocurrent characteristics in photo-CELIV experiments is discussed and shown to explain subtle features that were seen in former publications but were not yet assigned to electron trapping. Comparable studies on PFTBTT copolymers with chemical modifications of the side chains and backbone suggest that the observed electron trapping is not caused by a distinct chemical species but rather is related to interchain interactions.},
  language  = {en}
}