@article{BalintMartonSchatzetal.2018,
  author    = {Balint, Miklos and Marton, Orsolya and Schatz, Marlene and D{\"u}ring, Rolf-Alexander and Grossart, Hans-Peter},
  title     = {Proper experimental design requires randomization/balancing of molecular ecology experiments},
  series = {Ecology and evolution},
  volume    = {8},
  journal   = {Ecology and evolution},
  number    = {3},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {2045-7758},
  doi       = {10.1002/ece3.3687},
  pages     = {1786 -- 1793},
  year      = {2018},
  abstract  = {Properly designed (randomized and/or balanced) experiments are standard in ecological research. Molecular methods are increasingly used in ecology, but studies generally do not report the detailed design of sample processing in the laboratory. This may strongly influence the interpretability of results if the laboratory procedures do not account for the confounding effects of unexpected laboratory events. We demonstrate this with a simple experiment where unexpected differences in laboratory processing of samples would have biased results if randomization in DNA extraction and PCR steps do not provide safeguards. We emphasize the need for proper experimental design and reporting of the laboratory phase of molecular ecology research to ensure the reliability and interpretability of results.},
  language  = {en}
}
@misc{BalintPfenningerGrossartetal.2018,
  author    = {B{\´a}lint, Mikl{\´o}s and Pfenninger, Markus and Grossart, Hans-Peter and Taberlet, Pierre and Vellend, Mark and Leibold, Mathew A. and Englund, Goran and Bowler, Diana},
  title     = {Environmental DNA time series in ecology},
  series = {Trends in ecology \& evolution},
  volume    = {33},
  journal   = {Trends in ecology \& evolution},
  number    = {12},
  publisher = {Elsevier},
  address   = {London},
  issn      = {0169-5347},
  doi       = {10.1016/j.tree.2018.09.003},
  pages     = {945 -- 957},
  year      = {2018},
  abstract  = {Ecological communities change in time and space, but long-term dynamics at the century-to-millennia scale are poorly documented due to lack of relevant data sets. Nevertheless, understanding long-term dynamics is important for explaining present-day biodiversity patterns and placing conservation goals in a historical context. Here, we use recent examples and new perspectives to highlight how environmental DNA (eDNA) is starting to provide a powerful new source of temporal data for research questions that have so far been overlooked, by helping to resolve the ecological dynamics of populations, communities, and ecosystems over hundreds to thousands of years. We give examples of hypotheses that may be addressed by temporal eDNA biodiversity data, discuss possible research directions, and outline related challenges.},
  language  = {en}
}
@misc{BalintMartonSchatzetal.2018,
  author    = {B{\´a}lint, Mikl{\´o}s and M{\´a}rton, Orsolya and Schatz, Marlene and D{\"u}ring, Rolf-Alexander and Grossart, Hans-Peter},
  title     = {Proper experimental design requires randomization/balancing of molecular ecology experiments},
  series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  journal   = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  number    = {616},
  issn      = {1866-8372},
  doi       = {10.25932/publishup-42387},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-423878},
  pages     = {8},
  year      = {2018},
  abstract  = {Properly designed (randomized and/or balanced) experiments are standard in ecological research. Molecular methods are increasingly used in ecology, but studies generally do not report the detailed design of sample processing in the laboratory. This may strongly influence the interpretability of results if the laboratory procedures do not account for the confounding effects of unexpected laboratory events. We demonstrate this with a simple experiment where unexpected differences in laboratory processing of samples would have biased results if randomization in DNA extraction and PCR steps do not provide safeguards. We emphasize the need for proper experimental design and reporting of the laboratory phase of molecular ecology research to ensure the reliability and interpretability of results.},
  language  = {en}
}