@misc{WoodhouseMakowerYeungetal.2016,
  author    = {Woodhouse, Jason Nicholas and Makower, A. Katharina and Yeung, Anna C. Y. and Ongley, Sarah E. and Micallef, Melinda L. and Moffitt, Michelle C. and Neilan, Brett A.},
  title     = {Advances in genomics, transcriptomics and proteomics of toxin-producing cyanobacteria},
  series = {Environmental microbiology reports},
  volume    = {8},
  journal   = {Environmental microbiology reports},
  publisher = {Wiley-Blackwell},
  address   = {Hoboken},
  issn      = {1758-2229},
  doi       = {10.1111/1758-2229.12366},
  pages     = {3 -- 13},
  year      = {2016},
  abstract  = {A common misconception persists that the genomes of toxic and non-toxic cyanobacterial strains are largely conserved with the exception of the presence or absence of the genes responsible for toxin production. Implementation of -omics era technologies has challenged this paradigm, with comparative analyses providing increased insight into the differences between strains of the same species. The implementation of genomic, transcriptomic and proteomic approaches has revealed distinct profiles between toxin-producing and non-toxic strains. Further, metagenomics and metaproteomics highlight the genomic potential and functional state of toxic bloom events over time. In this review, we highlight how these technologies have shaped our understanding of the complex relationship between these molecules, their producers and the environment at large within which they persist.},
  language  = {en}
}
@article{MakowerSchuurmansGrothetal.2015,
  author    = {Makower, A. Katharina and Schuurmans, J. Merijn and Groth, Detlef and Zilliges, Yvonne and Matthijs, Hans C. P. and Dittmann-Th{\"u}nemann, Elke},
  title     = {Transcriptomics-Aided dissection of the intracellular and extracellular roles of microcystin in microcystis aeruginosa PCC 7806},
  series = {Applied and environmental microbiology},
  volume    = {81},
  journal   = {Applied and environmental microbiology},
  number    = {2},
  publisher = {American Society for Microbiology},
  address   = {Washington},
  issn      = {0099-2240},
  doi       = {10.1128/AEM.02601-14},
  pages     = {544 -- 554},
  year      = {2015},
  abstract  = {Recent studies have provided evidence for both intracellular and extracellular roles of the potent hepatotoxin microcystin (MC) in the bloom-forming cyanobacterium Microcystis. Here, we surveyed transcriptomes of the wild-type strain M. aeruginosa PCC 7806 and the microcystin-deficient Delta mcyB mutant under low light conditions with and without the addition of external MC of the LR variant (MC-LR). Transcriptomic data acquired by microarray and quantitative PCR revealed substantial differences in the relative expression of genes of the central intermediary metabolism, photosynthesis, and energy metabolism. In particular, the data provide evidence for a lower photosystem I (PSI)-to-photosystem II (PSII) ratio and a more pronounced carbon limitation in the microcystin-deficient mutant. Interestingly, only 6\% of the transcriptional differences could be complemented by external microcystin-LR addition. This MC signaling effect was seen exclusively for genes of the secondary metabolism category. The orphan polyketide synthase gene cluster IPF38-51 was specifically downregulated in response to external MC-LR under low light. Our data suggest a hierarchical and light-dependent cross talk of secondary metabolites and support both an intracellular and an extracellular role of MC in Microcystis.},
  language  = {en}
}