@article{BartholomaeusLipusMitzscherlingetal.2022,
  author    = {Bartholom{\"a}us, Alexander and Lipus, Daniel and Mitzscherling, Julia and MacLean, Joana and Wagner, Dirk},
  title     = {Draft Genome Sequence of Nocardioides alcanivorans NGK65(T), a Hexadecane-Degrading Bacterium},
  series = {Microbiology Resource Announcements},
  volume    = {11},
  journal   = {Microbiology Resource Announcements},
  number    = {8},
  publisher = {American Society for Microbiology},
  address   = {Washington},
  issn      = {2576-098X},
  doi       = {10.1128/mra.01213-21},
  pages     = {2},
  year      = {2022},
  abstract  = {The Gram-positive bacterium Nocardioides alcanivorans NGK65(T) was isolated from plastic-polluted soil and cultivated on medium with polyethylene as the single carbon source. Nanopore sequencing revealed the presence of candidate enzymes for the biodegradation of polyethylene. Here, we report the draft genome of this newly described member of the terrestrial plastisphere.},
  language  = {en}
}
@article{MitzscherlingMacLeanLipusetal.2022,
  author    = {Mitzscherling, Julia and MacLean, Joana and Lipus, Daniel and Bartholom{\"a}us, Alexander and Mangelsdorf, Kai and Lipski, Andr{\´e} and Roddatis, Vladimir and Liebner, Susanne and Wagner, Dirk},
  title     = {Nocardioides alcanivorans sp. nov., a novel hexadecane-degrading species isolated from plastic waste},
  series = {International journal of systematic and evolutionary microbiology},
  volume    = {72},
  journal   = {International journal of systematic and evolutionary microbiology},
  number    = {4},
  publisher = {Microbiology Society},
  address   = {London},
  issn      = {1466-5026},
  doi       = {10.1099/ijsem.0.005319},
  pages     = {11},
  year      = {2022},
  abstract  = {Strain NGK65(T), a novel hexadecane degrading, non-motile, Gram-positive, rod-to-coccus shaped, aerobic bacterium, was isolated from plastic polluted soil sampled at a landfill. Strain NGK65(T) hydrolysed casein, gelatin, urea and was catalase-positive. It optimally grew at 28 degrees C. in 0-1\% NaCl and at pH 7.5-8.0. Glycerol, D-glucose, arbutin, aesculin, salicin, potassium 5-ketogluconate. sucrose, acetate, pyruvate and hexadecane were used as sole carbon sources. The predominant membrane fatty acids were iso-C-16:0 followed by iso-C(17:)0 and C-18:1 omega 9c. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and hydroxyphosphatidylinositol. The cell-wall peptidoglycan type was A3 gamma, with LL-diaminopimelic acid and glycine as the diagnostic amino acids. MK 8 (H-4) was the predominant menaquinone. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NGK65(T) belongs to the genus Nocardioides (phylum Actinobacteria). appearing most closely related to Nocardioides daejeonensis MJ31(T) (98.6\%) and Nocardioides dubius KSL-104(T) (98.3\%). The genomic DNA G+C content of strain NGK65(T) was 68.2\%. Strain NGK65(T) and the type strains of species involved in the analysis had average nucleotide identity values of 78.3-71.9\% as well as digital DNA-DNA hybridization values between 22.5 and 19.7\%, which clearly indicated that the isolate represents a novel species within the genus Nocardioides. Based on phenotypic and molecular characterization, strain NGK65(T) can clearly be differentiated from its phylogenetic neighbours to establish a novel species, for which the name Nocardioides alcanivorans sp. nov. is proposed. The type strain is NGK65(T) (=DSM 113112(T)=NCCB 100846(T)).},
  language  = {en}
}
@article{MitzscherlingMacLeanLipusetal.2022,
  author    = {Mitzscherling, Julia and MacLean, Joana and Lipus, Daniel and Bartholom{\"a}us, Alexander and Mangelsdorf, Kai and Lipski, Andre and Roddatis, Vladimir and Liebner, Susanne and Wagner, Dirk},
  title     = {Paenalcaligenes niemegkensis sp. nov., a novel species of the family Alcaligenaceae isolated from plastic waste},
  series = {International journal of systematic and evolutionary microbiology},
  volume    = {72},
  journal   = {International journal of systematic and evolutionary microbiology},
  number    = {4},
  publisher = {Microbiology Society},
  address   = {London},
  issn      = {1466-5026},
  doi       = {10.1099/ijsem.0.005333},
  pages     = {9},
  year      = {2022},
  abstract  = {Strain NGK35T is a motile, Gram-stain-negative, rod-shaped (1.0-2.1 mu m long and 0.6-0.8 mu m wide), aerobic bacterium that was isolated from plastic-polluted landfill soil. The strain grew at temperatures between 6 and 37 degrees C (optimum, 28 degrees C), in 0-10 \% NaCl (optimum, 1 \%) and at pH 6.0-9.5 (optimum, pH 7.5-8.5). It was positive for cytochrome c oxidase, catalase as well as H2S production, and hydrolysed casein and urea. It used a variety of different carbon sources including citrate, lactate and pyruvate. The predominant membrane fatty acids were C-16:1 cis9 and C-16:0, followed by C-17:0 cyclo and C-18:1 cis11. The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine, followed by diphosphatidyglycerol. The only quinone was ubiquinone Q-8. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NGK35(T) belongs to the genus Paenalcaligenes (family Alcaligenaceae), appearing most closely related to Paenalcaligenes hominis CCUG 53761A(T) (96.90 \%) and Paenalcaligenes suwonensis ABC02-12(T) (96.94 \%). The genomic DNA G+C content of strain NGK35(T) was 52.1 mol\%. Genome-based calculations (genome-to-genome distance, average nucleotide identity and DNA G+C content) clearly indicated that the isolate represents a novel species within the genus Paenalcaligenes. Based on phenotypic and molecular characterization, strain NGK35(T) can clearly be differentiated from its phylogenetic neighbours establishing a novel species, for which the name Paenalcaligenes niemegkensis sp. nov. is proposed. The type strain is NGK35T (=DSM 113270(T)=NCCB 100854(T)).},
  language  = {en}
}
@article{BartholomaeusMitzscherlingLipusetal.2022,
  author    = {Bartholom{\"a}us, Alexander and Mitzscherling, Julia and Lipus, Daniel and MacLean, Joana and Wagner, Dirk},
  title     = {Draft genome sequence of Paenalcaligenes niemegkensis NGK35T, isolated from plastic-polluted soil of an abandoned landfill},
  series = {Microbiology Resource Announcements},
  volume    = {11},
  journal   = {Microbiology Resource Announcements},
  number    = {10},
  publisher = {American Society for Microbiology},
  address   = {Washington},
  issn      = {2576-098X},
  doi       = {10.1128/mra.00671-22},
  pages     = {2},
  year      = {2022},
  abstract  = {The Gram-negative bacterium Paenalcaligenes niemegkensis NGK35(T) was isolated from plastic debris in an abandoned landfill. It has the ability to grow on polyethylene and hexadecane as the sole carbon sources. Here, we report the corresponding draft genome, which contains 3.66 Mbp and is characterized by a G+C content of 52.1\%.},
  language  = {en}
}