@misc{SchmaelzlinDongenKlimantetal.2005, author = {Schm{\"a}lzlin, Elmar and Dongen, Joost T. van and Klimant, Ingo and Marmod{\´e}e, Bettina and Steup, Martin and Fishahn, Joachim and Geigenberger, Peter and L{\"o}hmannsr{\"o}ben, Hans-Gerd}, title = {An optical multifrequency phase-modulation method using microbeads for measuring intracellular oxygen concentrations in plants}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-12232}, year = {2005}, abstract = {A technique has been developed to measure absolute intracellular oxygen concentrations in green plants. Oxygen-sensitive phosphorescent microbeads were injected into the cells and an optical multifrequency phase-modulation technique was used to discriminate the sensor signal from the strong autofluorescence of the plant tissue. The method was established using photosynthesis-competent cells of the giant algae Chara corallina L., and was validated by application to various cell types of other plant species.}, language = {en} }