@article{LiangHeinrichSimardetal.2013, author = {Liang, Wei and Heinrich, Ingo and Simard, Sonia and Helle, Gerhard and Linan, Isabel Dorado and Heinken, Thilo}, title = {Climate signals derived from cell anatomy of Scots pine in NE Germany}, series = {Tree physiology}, volume = {33}, journal = {Tree physiology}, number = {8}, publisher = {Oxford Univ. Press}, address = {Oxford}, issn = {0829-318X}, doi = {10.1093/treephys/tpt059}, pages = {833 -- 844}, year = {2013}, abstract = {Tree-ring chronologies of Pinus sylvestris L. from latitudinal and altitudinal limits of the species distribution have been widely used for climate reconstructions, but there are many sites within the temperate climate zone, as is the case in northeastern Germany, at which there is little evidence of a clear climate signal in the chronologies. In this study, we developed long chronologies of several cell structure variables (e. g., average lumen area and cell wall thickness) from P. sylvestris growing in northeastern Germany and investigated the influence of climate on ring widths and cell structure variables. We found significant correlations between cell structure variables and temperature, and between tree-ring width and relative humidity and vapor pressure, respectively, enabling the development of robust reconstructions from temperate sites that have not yet been realized. Moreover, it has been shown that it may not be necessary to detrend chronologies of cell structure variables and thus low-frequency climate signals may be retrieved from longer cell structure chronologies. The relatively extensive resource of archaeological material of P. sylvestris covering approximately the last millennium may now be useful for climate reconstructions in northeastern Germany and other sites in the temperate climate zone.}, language = {en} } @article{LiangHeinrichHelleetal.2013, author = {Liang, Wei and Heinrich, Ingo and Helle, Gerhard and Linan, Isabel Dorado and Heinken, Thilo}, title = {Applying CLSM to increment core surfaces for histometric analyses a novel advance in quantitative wood anatomy}, series = {Dendrochronologia : an interdisciplinary journal of tree-ring science}, volume = {31}, journal = {Dendrochronologia : an interdisciplinary journal of tree-ring science}, number = {2}, publisher = {Elsevier}, address = {Jena}, issn = {1125-7865}, doi = {10.1016/j.dendro.2012.09.002}, pages = {140 -- 145}, year = {2013}, abstract = {A novel procedure has been developed to conduct cell structure measurements on increment core samples of conifers. The procedure combines readily available hardware and software equipment. The essential part of the procedure is the application of a confocal laser scanning microscope (CLSM) which captures images directly from increment cores surfaced with the advanced WSL core-microtome. Cell wall and lumen are displayed with a strong contrast due to the monochrome black and green nature of the images. Consecutive images are merged into long images representing entire increment cores which are then analysed for cell structures in suitable software.}, language = {en} }