@article{SallenAndraeLudygaetal.2020, author = {Sallen, Jeffrey and Andr{\"a}, Christian and Ludyga, Sebastian and M{\"u}cke, Manuel and Herrmann, Christian}, title = {School children's physical activity, motor competence, and corresponding self-perception}, series = {Journal of physical activity and health}, volume = {17}, journal = {Journal of physical activity and health}, number = {11}, publisher = {Human Kinetics Publ.}, address = {Champaign}, issn = {1543-3080}, doi = {10.1123/jpah.2019-0507}, pages = {1083 -- 1090}, year = {2020}, abstract = {Background: The relationship between engagement in physical activity and the development of motor competence (MC) is considered to be reciprocal and dynamic throughout childhood and adolescence. The 10-month follow-up study aimed to explore this reciprocal relationship and investigated whether the relationship is mediated by the corresponding self-perception of MC (PMC). Methods: A total of 51 children aged between 10 and 11 years (M = 10.27 [0.45]) participated in the study (52.9\% boys, 47.1\% girls). As an indicator for physical activity, the average vigorous physical activity (VPA) per day was measured by ActiGraph accelerometers. Two aspects of MC and PMC were recorded: self-movement and object movement. Saturated pathway models in a cross-lagged panel design with 2 measurement points were analyzed. Results: Reciprocal and direct relationships between VPA and MC object movement respectively MC self-movement were not found in longitudinal analyses with PMC as a mediator. Indirect effects of MC at t1 on VPA at t2 via PMC were identified (self-movement: beta = 0.13, 95\% confidence interval, 0.04 to 0.26; object movement: beta = 0.14, 95\% confidence interval, 0.01 to 0.49). Conclusion: The results highlight the importance of MC and PMC in promoting children's VPA. However, VPA does not drive the development of MC.}, language = {en} } @article{BoescheRogassLubitzetal.2015, author = {B{\"o}sche, Nina Kristine and Rogass, Christian and Lubitz, Christin and Brell, Maximilian and Herrmann, Sabrina and Mielke, Christian and Tonn, Sabine and Appelt, Oona and Altenberger, Uwe and Kaufmann, Hermann}, title = {Hyperspectral REE (Rare Earth Element) Mapping of Outcrops-Applications for Neodymium Detection}, series = {Remote sensing}, volume = {7}, journal = {Remote sensing}, number = {5}, publisher = {MDPI}, address = {Basel}, issn = {2072-4292}, doi = {10.3390/rs70505160}, pages = {5160 -- 5186}, year = {2015}, abstract = {In this study, an in situ application for identifying neodymium (Nd) enriched surface materials that uses multitemporal hyperspectral images is presented (HySpex sensor). Because of the narrow shape and shallow absorption depth of the neodymium absorption feature, a method was developed for enhancing and extracting the necessary information for neodymium from image spectra, even under illumination conditions that are not optimal. For this purpose, the two following approaches were developed: (1) reducing noise and analyzing changing illumination conditions by averaging multitemporal image scenes and (2) enhancing the depth of the desired absorption band by deconvolving every image spectrum with a Gaussian curve while the rest of the spectrum remains unchanged (Richardson-Lucy deconvolution). To evaluate these findings, nine field samples from the Fen complex in Norway were analyzed using handheld X-ray fluorescence devices and by conducting detailed laboratory-based geochemical rare earth element determinations. The result is a qualitative outcrop map that highlights zones that are enriched in neodymium. To reduce the influences of non-optimal illumination, particularly at the studied site, a minimum of seven single acquisitions is required. Sharpening the neodymium absorption band allows for robust mapping, even at the outer zones of enrichment. From the geochemical investigations, we found that iron oxides decrease the applicability of the method. However, iron-related absorption bands can be used as secondary indicators for sulfidic ore zones that are mainly enriched with rare earth elements. In summary, we found that hyperspectral spectroscopy is a noninvasive, fast and cost-saving method for determining neodymium at outcrop surfaces.}, language = {en} } @misc{BoescheRogassLubitzetal.2017, author = {B{\"o}sche, Nina Kristine and Rogass, Christian and Lubitz, Christin and Brell, Maximilian and Herrmann, Sabrina and Mielke, Christian and Tonn, Sabine and Appelt, Oona and Altenberger, Uwe and Kaufmann, Hermann}, title = {Hyperspectral REE (Rare Earth Element) mapping of outcrops}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-400171}, pages = {27}, year = {2017}, abstract = {In this study, an in situ application for identifying neodymium (Nd) enriched surface materials that uses multitemporal hyperspectral images is presented (HySpex sensor). Because of the narrow shape and shallow absorption depth of the neodymium absorption feature, a method was developed for enhancing and extracting the necessary information for neodymium from image spectra, even under illumination conditions that are not optimal. For this purpose, the two following approaches were developed: (1) reducing noise and analyzing changing illumination conditions by averaging multitemporal image scenes and (2) enhancing the depth of the desired absorption band by deconvolving every image spectrum with a Gaussian curve while the rest of the spectrum remains unchanged (Richardson-Lucy deconvolution). To evaluate these findings, nine field samples from the Fen complex in Norway were analyzed using handheld X-ray fluorescence devices and by conducting detailed laboratory-based geochemical rare earth element determinations. The result is a qualitative outcrop map that highlights zones that are enriched in neodymium. To reduce the influences of non-optimal illumination, particularly at the studied site, a minimum of seven single acquisitions is required. Sharpening the neodymium absorption band allows for robust mapping, even at the outer zones of enrichment. From the geochemical investigations, we found that iron oxides decrease the applicability of the method. However, iron-related absorption bands can be used as secondary indicators for sulfidic ore zones that are mainly enriched with rare earth elements. In summary, we found that hyperspectral spectroscopy is a noninvasive, fast and cost-saving method for determining neodymium at outcrop surfaces}, language = {en} } @misc{ManzoniCapekPoradaetal.2018, author = {Manzoni, Stefano and Capek, Petr and Porada, Philipp and Thurner, Martin and Winterdahl, Mattias and Beer, Christian and Bruchert, Volker and Frouz, Jan and Herrmann, Anke M. and Lindahl, Bjorn D. and Lyon, Steve W. and Šantrůčkov{\´a}, Hana and Vico, Giulia and Way, Danielle}, title = {Reviews and syntheses}, series = {Biogeosciences}, volume = {15}, journal = {Biogeosciences}, number = {19}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {1726-4170}, doi = {10.5194/bg-15-5929-2018}, pages = {5929 -- 5949}, year = {2018}, abstract = {The cycling of carbon (C) between the Earth surface and the atmosphere is controlled by biological and abiotic processes that regulate C storage in biogeochemical compartments and release to the atmosphere. This partitioning is quantified using various forms of C-use efficiency (CUE) - the ratio of C remaining in a system to C entering that system. Biological CUE is the fraction of C taken up allocated to biosynthesis. In soils and sediments, C storage depends also on abiotic processes, so the term C-storage efficiency (CSE) can be used. Here we first review and reconcile CUE and CSE definitions proposed for autotrophic and heterotrophic organisms and communities, food webs, whole ecosystems and watersheds, and soils and sediments using a common mathematical framework. Second, we identify general CUE patterns; for example, the actual CUE increases with improving growth conditions, and apparent CUE decreases with increasing turnover. We then synthesize > 5000CUE estimates showing that CUE decreases with increasing biological and ecological organization - from uni-cellular to multicellular organisms and from individuals to ecosystems. We conclude that CUE is an emergent property of coupled biological-abiotic systems, and it should be regarded as a flexible and scale-dependent index of the capacity of a given system to effectively retain C.}, language = {en} } @misc{QuiterioMartinsOnofreetal.2018, author = {Quiterio, Ana and Martins, Joao and Onofre, Marcos and Costa, Joao and Rodrigues, Joao Mota and Gerlach, Erin and Scheur, Claude and Herrmann, Christian}, title = {MOBAK 1 assessment in primary physical education}, series = {Perceptual \& motor skills}, volume = {125}, journal = {Perceptual \& motor skills}, number = {6}, publisher = {Sage Publ.}, address = {Thousand Oaks}, issn = {0031-5125}, doi = {10.1177/0031512518804358}, pages = {1055 -- 1069}, year = {2018}, abstract = {Children's motor competence is known to have a determinant role in learning and engaging later in complex motor skills and, thus, in physical activity. The development of adequate motor competence is a central aim of physical education, and assuring that pupils are learning and developing motor competence depends on accurate assessment protocols. The MOBAK 1 test battery is a recent instrument developed to assess motor competence in primary physical education. This study used the MOBAK 1 to explore motor competence levels and gender differences among 249 (Mage = 6.3, SD = 0.5 years; 127 girls and 122 boys) Grade 1 primary school Portuguese children. On independent sample t tests, boys presented higher object movement motor competence than girls (boys: M = 5.8, SD = 1.7; girls: M = 4.0, SD = 1.7; p < .001), while girls were more proficient among self-movement skills (girls: M = 5.1, SD = 1.8; boys: M = 4.3, SD = 1.7; p < .01). On "total motor competence," boys (M = 10.3, SD = 2.6) averaged one point ahead of girls (M = 9.1, SD = 2.9). The percentage of girls in the first quartile of object movement was 18.9\%, while, for "self movement," the percentage of boys in the first quartile was almost double that of girls (30.3\% and 17.3\%, respectively). The confirmatory model to test for construct validity confirmed the assumed theoretical two-factor structure of MOBAK 1 test items in this Portuguese sample. These results support the MOBAK 1 instrument for assessing motor competence and highlighted gender differences, of relevance to intervention efforts.}, language = {en} } @misc{BierbachSchulteHerrmannetal.2011, author = {Bierbach, David and Schulte, Matthias and Herrmann, Nina and Tobler, Michael and Stadler, Stefan and Jung, Christian T. and Kunkel, Benjamin and Riesch, R{\"u}diger and Klaus, Sebastian and Ziege, Madlen and Indy, Jeane Rimber and Arias-Rodriguez, Lenin and Plath, Martin}, title = {Predator-induced changes of female mating preferences}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, number = {984}, issn = {1866-8372}, doi = {10.25932/publishup-43109}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-431099}, pages = {12}, year = {2011}, abstract = {Background In many species males face a higher predation risk than females because males display elaborate traits that evolved under sexual selection, which may attract not only females but also predators. Females are, therefore, predicted to avoid such conspicuous males under predation risk. The present study was designed to investigate predator-induced changes of female mating preferences in Atlantic mollies (Poecilia mexicana). Males of this species show a pronounced polymorphism in body size and coloration, and females prefer large, colorful males in the absence of predators. Results In dichotomous choice tests predator-na{\"i}ve (lab-reared) females altered their initial preference for larger males in the presence of the cichlid Cichlasoma salvini, a natural predator of P. mexicana, and preferred small males instead. This effect was considerably weaker when females were confronted visually with the non-piscivorous cichlid Vieja bifasciata or the introduced non-piscivorous Nile tilapia (Oreochromis niloticus). In contrast, predator experienced (wild-caught) females did not respond to the same extent to the presence of a predator, most likely due to a learned ability to evaluate their predators' motivation to prey. Conclusions Our study highlights that (a) predatory fish can have a profound influence on the expression of mating preferences of their prey (thus potentially affecting the strength of sexual selection), and females may alter their mate choice behavior strategically to reduce their own exposure to predators. (b) Prey species can evolve visual predator recognition mechanisms and alter their mate choice only when a natural predator is present. (c) Finally, experiential effects can play an important role, and prey species may learn to evaluate the motivational state of their predators.}, language = {en} } @phdthesis{Herrmann2017, author = {Herrmann, Christian}, title = {Motorische Basiskompetenzen - Konstrukt, Erfassung und Forschungsstand}, school = {Universit{\"a}t Potsdam}, pages = {47, 269}, year = {2017}, language = {de} } @article{RistowHerrmannIlligetal.2006, author = {Ristow, Michael and Herrmann, Andreas and Illig, Hubert and Klemm, Gunther and Kummer, Volker and Kl{\"a}ge, Hans-Christian and Machatzi, Bernd and Raetzel, Stefan and Schwarz, R. and Zimmermann, Friedrich}, title = {Liste und Rote Liste der etablierten Gef{\"a}ßpflanzen Brandenburgs}, year = {2006}, language = {de} } @article{SallenHirschmannHerrmann2018, author = {Sallen, Jeffrey and Hirschmann, Florian and Herrmann, Christian}, title = {Evaluation and Adaption of the Trier Inventory for Chronic Stress (TICS) for Assessment in Competitive Sports}, series = {Frontiers in psychology}, volume = {9}, journal = {Frontiers in psychology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-1078}, doi = {10.3389/fpsyg.2018.00308}, pages = {12}, year = {2018}, abstract = {The demands of a career in competitive sports can lead to chronic stress perception among athletes if there is a non-conformity of requirements and available coping resources. The Trier Inventory for Chronic Stress (TICS) (Schulz et al., 2004) is said to be thoroughly validated. Nevertheless, it has not yet been subjected to a confirmatory factor analysis. The present study aims (1) to evaluate the factorial validity of the TICS within the context of competitive sports and (2) to adapt a short version (TICS-36). The total sample consisted of 564 athletes (age in years: M = 19.1, SD = 3.70). The factor structure of the original TICS did not adequately fit the present data, whereas the short version presented a satisfactory fit. The results indicate that the TICS-36 is an economical instrument for gathering interpretable information about chronic stress. For assessment in competitive sports with TICS-36, we generated overall and gender-specific norm values.}, language = {en} } @article{SteinbergOuerghemmiHerrmannetal.2010, author = {Steinberg, Christian E. W. and Ouerghemmi, Nadia and Herrmann, Steffen and Bouchnak, Rihab and Timofeyev, Maxim A. and Menzel, Ralph}, title = {Stress by poor food quality and exposure to humic substances : daphnia magna responds with oxidative stress, lifespan extension, but reduced offspring numbers}, issn = {0018-8158}, doi = {10.1007/s10750-010-0334-4}, year = {2010}, abstract = {In freshwater systems, many abiotic and biotic factors determine the natural fluctuation of Daphnia spec. populations: climatic and water quality parameters, quantitative and qualitative food quality and quantity, predation, and humic substances. Many factors/stressors act in concert. In this contribution, we supplied Daphnia magna with two different diets (chlorococcal alga Pseudokirchneriella subcapitata and baker's yeast) fed ad libitum and exposed it to an environmentally realistic concentration of humic substances (HSs). Exposure to HSs caused a transcriptionally controlled stress response with studied genes; cat and hsp60, for the latter partial sequences have been identified. Furthermore, the exposure to HSs reduced the antioxidant capacity. Yet, a much stronger oxidative stress is caused by feeding yeast, which reduced the anti-oxidative capacity to values of approximately 50\% of the green algal diet. This reduction is most likely due to the yeast's cell wall to resist digestion rather than to the elemental ratio or deficiency in long-chained unsaturated fatty acids, because both diets were deficient in fatty acids with back bones of more than 20 C-atoms. We assume that the biochemical machinery in the gut continuously activated oxygen to cleave the yeast's cell wall and, hence, reduced the antioxidative capacity of the animals. Neither the analyzed oxidant, H2O2, nor the antioxidants, total apparent ascorbic acid nor free proline, reflected the oxidative stress situations properly. In addition to the stress, HS exposure extended the mean lifespan of algae-fed D. magna, but at the expense of offspring numbers; so did also the pure yeast diet as compared to the algae diet. The first lifespan extension can be explained by the potential of HSs to block the pathway via the insulin-like growth factor 1 (IGF), whereas the second matches the, in aging papers, well described, but mechanistically poorly understood caloric restriction. Yeast-fed animals, exposed to HSs changed the energy allocation by reducing life span, but increasing offspring numbers. With the lifespan and offspring numbers, ecologically relevant parameters are differently affected by the simultaneous action of two environmentally relevant stressors.}, language = {en} } @article{BierbachSchulteHerrmannetal.2011, author = {Bierbach, David and Schulte, Matthias and Herrmann, Nina and Tobler, Michael and Stadler, Stefan and Jung, Christian T. and Kunkel, Benjamin and Riesch, R{\"u}diger and Klaus, Sebastian and Ziege, Madlen and Rimber Indy, Jeane and Arias-Rodriguez, Lenin and Plath, Martin}, title = {Predator-induced changes of female mating preferences innate and experiential effects}, series = {BMC evolutionary biology}, volume = {11}, journal = {BMC evolutionary biology}, number = {3-4}, publisher = {BioMed Central}, address = {London}, issn = {1471-2148}, doi = {10.1186/1471-2148-11-190}, pages = {10}, year = {2011}, abstract = {Background: In many species males face a higher predation risk than females because males display elaborate traits that evolved under sexual selection, which may attract not only females but also predators. Females are, therefore, predicted to avoid such conspicuous males under predation risk. The present study was designed to investigate predator-induced changes of female mating preferences in Atlantic mollies (Poecilia mexicana). Males of this species show a pronounced polymorphism in body size and coloration, and females prefer large, colorful males in the absence of predators. Results: In dichotomous choice tests predator-naive (lab-reared) females altered their initial preference for larger males in the presence of the cichlid Cichlasoma salvini, a natural predator of P. mexicana, and preferred small males instead. This effect was considerably weaker when females were confronted visually with the non-piscivorous cichlid Vieja bifasciata or the introduced non-piscivorous Nile tilapia (Oreochromis niloticus). In contrast, predator experienced (wild-caught) females did not respond to the same extent to the presence of a predator, most likely due to a learned ability to evaluate their predators' motivation to prey. Conclusions: Our study highlights that (a) predatory fish can have a profound influence on the expression of mating preferences of their prey (thus potentially affecting the strength of sexual selection), and females may alter their mate choice behavior strategically to reduce their own exposure to predators. (b) Prey species can evolve visual predator recognition mechanisms and alter their mate choice only when a natural predator is present. (c) Finally, experiential effects can play an important role, and prey species may learn to evaluate the motivational state of their predators.}, language = {en} } @article{SchroederFuerstKlasenetal.2000, author = {Schr{\"o}der, Rolf and F{\"u}rst, Dieter Oswald and Klasen, Christian and Reiman, Jens and Herrmann, Harald and VanDerVen, Peter F. M.}, title = {The association of plectin with Z-discs is a prerequisite for the formation of the intermyofibrillar desmin cytoskeleton}, year = {2000}, language = {en} } @article{SchroederWarloHerrmannetal.1999, author = {Schr{\"o}der, Rolf and Warlo, Irene and Herrmann, Harald and VanDerVen, Peter F. M and Klasen, Christian and Bl{\"u}mke, Ingmar and Mundegar, Rustam R. and F{\"u}rst, Dieter Oswald and G{\"o}bel, Hans H. and Magin, Thomas}, title = {Immunogold EM reveals a close association of plectin and the desmin cytoskeleton in human skeletal muscle}, year = {1999}, language = {en} } @misc{ManzoniČapekPoradaetal.2018, author = {Manzoni, Stefano and Čapek, Petr and Porada, Philipp and Thurner, Martin and Winterdahl, Mattias and Beer, Christian and Br{\"u}chert, Volker and Frouz, Jan and Herrmann, Anke M. and Lindahl, Bj{\"o}rn D. and Lyon, Steve W. and Šantrůčkov{\´a}, Hana and Vico, Giulia and Way, Danielle}, title = {Reviews and syntheses}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, number = {1134}, issn = {1866-8372}, doi = {10.25932/publishup-44638}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-446386}, pages = {23}, year = {2018}, abstract = {The cycling of carbon (C) between the Earth surface and the atmosphere is controlled by biological and abiotic processes that regulate C storage in biogeochemical compartments and release to the atmosphere. This partitioning is quantified using various forms of C-use efficiency (CUE) - the ratio of C remaining in a system to C entering that system. Biological CUE is the fraction of C taken up allocated to biosynthesis. In soils and sediments, C storage depends also on abiotic processes, so the term C-storage efficiency (CSE) can be used. Here we first review and reconcile CUE and CSE definitions proposed for autotrophic and heterotrophic organisms and communities, food webs, whole ecosystems and watersheds, and soils and sediments using a common mathematical framework. Second, we identify general CUE patterns; for example, the actual CUE increases with improving growth conditions, and apparent CUE decreases with increasing turnover. We then synthesize > 5000CUE estimates showing that CUE decreases with increasing biological and ecological organization - from uni-cellular to multicellular organisms and from individuals to ecosystems. We conclude that CUE is an emergent property of coupled biological-abiotic systems, and it should be regarded as a flexible and scale-dependent index of the capacity of a given system to effectively retain C.}, language = {en} } @misc{AichertStaigerSchulteMaeteretal.2010, author = {Aichert, Ingrid and Staiger, Anja and Schulte-M{\"a}ter, Anne and Becker-Redding, Ulrike and Stahn, Corinna and Peschke, Claudia and Heide, Judith and Ott, Susan and Herrmann, Heike and V{\"o}lsch, Juliane and Mayer, J{\"o}rg and Rohnke, Lucie and Frank, Ulrike and Stadie, Nicole and Jentsch, Nadine and Blech, Anke and Kurtenbach, Stephanie and Thieke, Johanna and Schr{\"o}der, Astrid and Stahn, Corinna and H{\"o}rnig, Robin and Burchert, Frank and De Bleser, Ria and Heister, Julian and Bartels, Luise and W{\"u}rzner, Kay-Michael and B{\"o}hme, Romy and Burmester, Juliane and Krajewski, Melanie and Nager, Wido and Jungeh{\"u}lsing, Gerhard Jan and Wartenburger, Isabell and J{\"o}bges, Michael and Schwilling, Eleonore and Lidzba, Karen and Winkler, Susanne and Konietzko, Andreas and Kr{\"a}geloh-Mann, Ingeborg and Rilling, Eva and Wilken, Rainer and Wismann, Kathrin and Glandorf, Birte and Hoffmann, Hannah and Hinnenkamp, Christiane and Rohlmann, Insa and Ludewigt, Jacqueline and Bittner, Christian and Orlov, Tatjana and Claus, Katrin and Ehemann, Christine and Winnecken, Andreas and Hummel, Katja and Breitenstein, Sarah}, title = {Spektrum Patholinguistik = Schwerpunktthema: Von der Programmierung zur Artikulation : Sprechapraxie bei Kindern und Erwachsenen}, number = {3}, editor = {Wahl, Michael and Stahn, Corinna and Hanne, Sandra and Fritzsche, Tom}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, organization = {Verband f{\"u}r Patholinguistik e. V. (vpl)}, isbn = {978-3-86956-079-3}, issn = {1869-3822}, doi = {10.25932/publishup-4578}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-45470}, year = {2010}, abstract = {Das 3. Herbsttreffen Patholinguistik fand am 21. November 2009 an der Universit{\"a}t Potsdam statt. Der vorliegende Tagungsband enth{\"a}lt die drei Hauptvortr{\"a}ge zum Schwerpunktthema „Von der Programmierung zu Artikulation: Sprechapraxie bei Kindern und Erwachsenen". Dar{\"u}ber hinaus enth{\"a}lt der Band die Beitr{\"a}ge aus dem Spektrum Patholinguistik, sowie die Abstracts der Posterpr{\"a}sentationen.}, language = {de} } @article{WelkeSperberBergmannetal.2022, author = {Welke, Robert-William and Sperber, Hannah Sabeth and Bergmann, Ronny and Koikkarah, Amit and Menke, Laura and Sieben, Christian and Kr{\"u}ger, Detlev H. and Chiantia, Salvatore and Herrmann, Andreas and Schwarzer, Roland}, title = {Characterization of hantavirus N protein intracellular dynamics and localization}, series = {Viruses}, volume = {14}, journal = {Viruses}, number = {3}, publisher = {MDPI}, address = {Basel}, issn = {1999-4915}, doi = {10.3390/v14030457}, pages = {14}, year = {2022}, abstract = {Hantaviruses are enveloped viruses that possess a tri-segmented, negative-sense RNA genome. The viral S-segment encodes the multifunctional nucleocapsid protein (N), which is involved in genome packaging, intracellular protein transport, immunoregulation, and several other crucial processes during hantavirus infection. In this study, we generated fluorescently tagged N protein constructs derived from Puumalavirus (PUUV), the dominant hantavirus species in Central, Northern, and Eastern Europe. We comprehensively characterized this protein in the rodent cell line CHO-K1, monitoring the dynamics of N protein complex formation and investigating co-localization with host proteins as well as the viral glycoproteins Gc and Gn. We observed formation of large, fibrillar PUUV N protein aggregates, rapidly coalescing from early punctate and spike-like assemblies. Moreover, we found significant spatial correlation of N with vimentin, actin, and P-bodies but not with microtubules. N constructs also co-localized with Gn and Gc albeit not as strongly as the glycoproteins associated with each other. Finally, we assessed oligomerization of N constructs, observing efficient and concentration-dependent multimerization, with complexes comprising more than 10 individual proteins.}, language = {en} }