@misc{DelfanVahedBishopetal.2022, author = {Delfan, Maryam and Vahed, Alieh and Bishop, David and Juybari, Raheleh Amadeh and Laher, Ismail and Saeidi, Ayoub and Granacher, Urs and Zouhal, Hassane}, title = {Effects of two workload-matched high intensity interval training protocols on regulatory factors associated with mitochondrial biogenesis in the soleus muscle of diabetic rats}, series = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, journal = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, issn = {1866-8364}, doi = {10.25932/publishup-56444}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-564441}, pages = {1 -- 12}, year = {2022}, abstract = {Aims: High intensity interval training (HIIT) improves mitochondrial characteristics. This study compared the impact of two workload-matched high intensity interval training (HIIT) protocols with different work:recovery ratios on regulatory factors related to mitochondrial biogenesis in the soleus muscle of diabetic rats. Materials and methods: Twenty-four Wistar rats were randomly divided into four equal-sized groups: non-diabetic control, diabetic control (DC), diabetic with long recovery exercise [4-5 × 2-min running at 80\%-90\% of the maximum speed reached with 2-min of recovery at 40\% of the maximum speed reached (DHIIT1:1)], and diabetic with short recovery exercise (5-6 × 2-min running at 80\%-90\% of the maximum speed reached with 1-min of recovery at 30\% of the maximum speed reached [DHIIT2:1]). Both HIIT protocols were completed five times/week for 4 weeks while maintaining equal running distances in each session. Results: Gene and protein expressions of PGC-1α, p53, and citrate synthase of the muscles increased significantly following DHIIT1:1 and DHIIT2:1 compared to DC (p ˂ 0.05). Most parameters, except for PGC-1α protein (p = 0.597), were significantly higher in DHIIT2:1 than in DHIIT1:1 (p ˂ 0.05). Both DHIIT groups showed significant increases in maximum speed with larger increases in DHIIT2:1 compared with DHIIT1:1. Conclusion: Our findings indicate that both HIIT protocols can potently up-regulate gene and protein expression of PGC-1α, p53, and CS. However, DHIIT2:1 has superior effects compared with DHIIT1:1 in improving mitochondrial adaptive responses in diabetic rats.}, language = {en} } @misc{DelfanJuybariGorganiFiruzjaeeetal.2022, author = {Delfan, Maryam and Juybari, Raheleh Amadeh and Gorgani-Firuzjaee, Sattar and Nielsen, Jens H{\o}iriis and Delfan, Neda and Laher, Ismail and Saeidi, Ayoub and Granacher, Urs and Zouhal, Hassane}, title = {High-Intensity Interval Training Improves Cardiac Function by miR-206 Dependent HSP60 Induction in Diabetic Rats}, series = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, journal = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, number = {802}, issn = {1866-8364}, doi = {10.25932/publishup-56723}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-567238}, pages = {11}, year = {2022}, abstract = {Objective: A role for microRNAs is implicated in several biological and pathological processes. We investigated the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on molecular markers of diabetic cardiomyopathy in rats. Methods: Eighteen male Wistar rats (260 ± 10 g; aged 8 weeks) with streptozotocin (STZ)-induced type 1 diabetes mellitus (55 mg/kg, IP) were randomly allocated to three groups: control, MICT, and HIIT. The two different training protocols were performed 5 days each week for 5 weeks. Cardiac performance (end-systolic and end-diastolic dimensions, ejection fraction), the expression of miR-206, HSP60, and markers of apoptosis (cleaved PARP and cytochrome C) were determined at the end of the exercise interventions. Results: Both exercise interventions (HIIT and MICT) decreased blood glucose levels and improved cardiac performance, with greater changes in the HIIT group (p < 0.001, η2: 0.909). While the expressions of miR-206 and apoptotic markers decreased in both training protocols (p < 0.001, η2: 0.967), HIIT caused greater reductions in apoptotic markers and produced a 20\% greater reduction in miR-206 compared with the MICT protocol (p < 0.001). Furthermore, both training protocols enhanced the expression of HSP60 (p < 0.001, η2: 0.976), with a nearly 50\% greater increase in the HIIT group compared with MICT. Conclusions: Our results indicate that both exercise protocols, HIIT and MICT, have the potential to reduce diabetic cardiomyopathy by modifying the expression of miR-206 and its downstream targets of apoptosis. It seems however that HIIT is even more effective than MICT to modulate these molecular markers.}, language = {en} } @misc{BouamraZouhalRateletal.2022, author = {Bouamra, Marwa and Zouhal, Hassane and Ratel, S{\´e}bastien and Makhlouf, Issam and Bezrati, Ikram and Chtara, Moktar and Behm, David George and Granacher, Urs and Chaouachi, Anis}, title = {Concurrent Training Promotes Greater Gains on Body Composition and Components of Physical Fitness Than Single-Mode Training (Endurance or Resistance) in Youth With Obesity}, series = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, journal = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, issn = {1866-8364}, doi = {10.25932/publishup-56397}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-563974}, pages = {1 -- 16}, year = {2022}, abstract = {The prevalence of obesity in the pediatric population has become a major public health issue. Indeed, the dramatic increase of this epidemic causes multiple and harmful consequences, Physical activity, particularly physical exercise, remains to be the cornerstone of interventions against childhood obesity. Given the conflicting findings with reference to the relevant literature addressing the effects of exercise on adiposity and physical fitness outcomes in obese children and adolescents, the effect of duration-matched concurrent training (CT) [50\% resistance (RT) and 50\% high-intensity-interval-training (HIIT)] on body composition and physical fitness in obese youth remains to be elucidated. Thus, the purpose of this study was to examine the effects of 9-weeks of CT compared to RT or HIIT alone, on body composition and selected physical fitness components in healthy sedentary obese youth. Out of 73 participants, only 37; [14 males and 23 females; age 13.4 ± 0.9 years; body-mass-index (BMI): 31.2 ± 4.8 kg·m-2] were eligible and randomized into three groups: HIIT (n = 12): 3-4 sets×12 runs at 80-110\% peak velocity, with 10-s passive recovery between bouts; RT (n = 12): 6 exercises; 3-4 sets × 10 repetition maximum (RM) and CT (n = 13): 50\% serial completion of RT and HIIT. CT promoted significant greater gains compared to HIIT and RT on body composition (p < 0.01, d = large), 6-min-walking test distance (6 MWT-distance) and on 6 MWT-VO2max (p < 0.03, d = large). In addition, CT showed substantially greater improvements than HIIT in the medicine ball throw test (20.2 vs. 13.6\%, p < 0.04, d = large). On the other hand, RT exhibited significantly greater gains in relative hand grip strength (p < 0.03, d = large) and CMJ (p < 0.01, d = large) than HIIT and CT. CT promoted greater benefits for fat, body mass loss and cardiorespiratory fitness than HIIT or RT modalities. This study provides important information for practitioners and therapists on the application of effective exercise regimes with obese youth to induce significant and beneficial body composition changes. The applied CT program and the respective programming parameters in terms of exercise intensity and volume can be used by practitioners as an effective exercise treatment to fight the pandemic overweight and obesity in youth.}, language = {en} } @article{BouamraZouhalRateletal.2022, author = {Bouamra, Marwa and Zouhal, Hassane and Ratel, S{\´e}bastien and Makhlouf, Issam and Bezrati, Ikram and Chtara, Moktar and Behm, David George and Granacher, Urs and Chaouachi, Anis}, title = {Concurrent Training Promotes Greater Gains on Body Composition and Components of Physical Fitness Than Single-Mode Training (Endurance or Resistance) in Youth With Obesity}, series = {Frontiers in Physiology}, volume = {13}, journal = {Frontiers in Physiology}, publisher = {Frontiers}, address = {Lausanne, Schweiz}, issn = {1664-042X}, doi = {10.3389/fphys.2022.869063}, pages = {1 -- 16}, year = {2022}, abstract = {The prevalence of obesity in the pediatric population has become a major public health issue. Indeed, the dramatic increase of this epidemic causes multiple and harmful consequences, Physical activity, particularly physical exercise, remains to be the cornerstone of interventions against childhood obesity. Given the conflicting findings with reference to the relevant literature addressing the effects of exercise on adiposity and physical fitness outcomes in obese children and adolescents, the effect of duration-matched concurrent training (CT) [50\% resistance (RT) and 50\% high-intensity-interval-training (HIIT)] on body composition and physical fitness in obese youth remains to be elucidated. Thus, the purpose of this study was to examine the effects of 9-weeks of CT compared to RT or HIIT alone, on body composition and selected physical fitness components in healthy sedentary obese youth. Out of 73 participants, only 37; [14 males and 23 females; age 13.4 ± 0.9 years; body-mass-index (BMI): 31.2 ± 4.8 kg·m-2] were eligible and randomized into three groups: HIIT (n = 12): 3-4 sets×12 runs at 80-110\% peak velocity, with 10-s passive recovery between bouts; RT (n = 12): 6 exercises; 3-4 sets × 10 repetition maximum (RM) and CT (n = 13): 50\% serial completion of RT and HIIT. CT promoted significant greater gains compared to HIIT and RT on body composition (p < 0.01, d = large), 6-min-walking test distance (6 MWT-distance) and on 6 MWT-VO2max (p < 0.03, d = large). In addition, CT showed substantially greater improvements than HIIT in the medicine ball throw test (20.2 vs. 13.6\%, p < 0.04, d = large). On the other hand, RT exhibited significantly greater gains in relative hand grip strength (p < 0.03, d = large) and CMJ (p < 0.01, d = large) than HIIT and CT. CT promoted greater benefits for fat, body mass loss and cardiorespiratory fitness than HIIT or RT modalities. This study provides important information for practitioners and therapists on the application of effective exercise regimes with obese youth to induce significant and beneficial body composition changes. The applied CT program and the respective programming parameters in terms of exercise intensity and volume can be used by practitioners as an effective exercise treatment to fight the pandemic overweight and obesity in youth.}, language = {en} } @article{DelfanVahedBishopetal.2022, author = {Delfan, Maryam and Vahed, Alieh and Bishop, David and Juybari, Raheleh Amadeh and Laher, Ismail and Saeidi, Ayoub and Granacher, Urs and Zouhal, Hassane}, title = {Effects of two workload-matched high-intensity interval training protocols on regulatory factors associated with mitochondrial biogenesis in the soleus muscle of diabetic rats}, series = {Frontiers in Physiology}, journal = {Frontiers in Physiology}, publisher = {Frontiers}, address = {Lausanne, Schweiz}, issn = {1664-042X}, doi = {10.3389/fphys.2022.927969}, pages = {1 -- 12}, year = {2022}, abstract = {Aims: High intensity interval training (HIIT) improves mitochondrial characteristics. This study compared the impact of two workload-matched high intensity interval training (HIIT) protocols with different work:recovery ratios on regulatory factors related to mitochondrial biogenesis in the soleus muscle of diabetic rats. Materials and methods: Twenty-four Wistar rats were randomly divided into four equal-sized groups: non-diabetic control, diabetic control (DC), diabetic with long recovery exercise [4-5 × 2-min running at 80\%-90\% of the maximum speed reached with 2-min of recovery at 40\% of the maximum speed reached (DHIIT1:1)], and diabetic with short recovery exercise (5-6 × 2-min running at 80\%-90\% of the maximum speed reached with 1-min of recovery at 30\% of the maximum speed reached [DHIIT2:1]). Both HIIT protocols were completed five times/week for 4 weeks while maintaining equal running distances in each session. Results: Gene and protein expressions of PGC-1α, p53, and citrate synthase of the muscles increased significantly following DHIIT1:1 and DHIIT2:1 compared to DC (p ˂ 0.05). Most parameters, except for PGC-1α protein (p = 0.597), were significantly higher in DHIIT2:1 than in DHIIT1:1 (p ˂ 0.05). Both DHIIT groups showed significant increases in maximum speed with larger increases in DHIIT2:1 compared with DHIIT1:1. Conclusion: Our findings indicate that both HIIT protocols can potently up-regulate gene and protein expression of PGC-1α, p53, and CS. However, DHIIT2:1 has superior effects compared with DHIIT1:1 in improving mitochondrial adaptive responses in diabetic rats.}, language = {en} } @misc{AlouiTayechArbiMejrietal.2022, author = {Aloui, Ali and Tayech, Amel and Arbi Mejri, Mohamed and Makhlouf, Issam and Clark, Cain C. T. and Granacher, Urs and Zouhal, Hassane and Ben Abderrahman, Abderraouf}, title = {Reliability and Validity of a New Taekwondo-Specific Change-of-Direction Speed Test With Striking Techniques in Elite Taekwondo Athletes: A Pilot Study}, series = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, journal = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, issn = {1866-8364}, doi = {10.25932/publishup-56319}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-563192}, pages = {1 -- 15}, year = {2022}, abstract = {The purpose of this study was to examine the test-retest reliability, and convergent and discriminative validity of a new taekwondo-specific change-of-direction (COD) speed test with striking techniques (TST) in elite taekwondo athletes. Twenty (10 males and 10 females) elite (athletes who compete at national level) and top-elite (athletes who compete at national and international level) taekwondo athletes with an average training background of 8.9 ± 1.3 years of systematic taekwondo training participated in this study. During the two-week test-retest period, various generic performance tests measuring COD speed, balance, speed, and jump performance were carried out during the first week and as a retest during the second week. Three TST trials were conducted with each athlete and the best trial was used for further analyses. The relevant performance measure derived from the TST was the time with striking penalty (TST-TSP). TST-TSP performances amounted to 10.57 ± 1.08 s for males and 11.74 ± 1.34 s for females. The reliability analysis of the TST performance was conducted after logarithmic transformation, in order to address the problem of heteroscedasticity. In both groups, the TST demonstrated a high relative test-retest reliability (intraclass correlation coefficients and 90\% compatibility limits were 0.80 and 0.47 to 0.93, respectively). For absolute reliability, the TST's typical error of measurement (TEM), 90\% compatibility limits, and magnitudes were 4.6\%, 3.4 to 7.7, for males, and 5.4\%, 3.9 to 9.0, for females. The homogeneous sample of taekwondo athletes meant that the TST's TEM exceeded the usual smallest important change (SIC) with 0.2 effect size in the two groups. The new test showed mostly very large correlations with linear sprint speed (r = 0.71 to 0.85) and dynamic balance (r = -0.71 and -0.74), large correlations with COD speed (r = 0.57 to 0.60) and vertical jump performance (r = -0.50 to -0.65), and moderate correlations with horizontal jump performance (r = -0.34 to -0.45) and static balance (r = -0.39 to -0.44). Top-elite athletes showed better TST performances than elite counterparts. Receiver operating characteristic analysis indicated that the TST effectively discriminated between top-elite and elite taekwondo athletes. In conclusion, the TST is a valid, and sensitive test to evaluate the COD speed with taekwondo specific skills, and reliable when considering ICC and TEM. Although the usefulness of the TST is questioned to detect small performance changes in the present population, the TST can detect moderate changes in taekwondo-specific COD speed.}, language = {en} } @article{AlouiTayechArbiMejrietal.2022, author = {Aloui, Ali and Tayech, Amel and Arbi Mejri, Mohamed and Makhlouf, Issam and Clark, Cain C. T. and Granacher, Urs and Zouhal, Hassane and Ben Abderrahman, Abderraouf}, title = {Reliability and Validity of a New Taekwondo-Specific Change-of-Direction Speed Test With Striking Techniques in Elite Taekwondo Athletes: A Pilot Study}, series = {Frontiers in Physiology}, volume = {13}, journal = {Frontiers in Physiology}, publisher = {Frontiers}, address = {Lausanne, Schweiz}, issn = {1664-042X}, doi = {10.3389/fphys.2022.774546}, pages = {1 -- 15}, year = {2022}, abstract = {The purpose of this study was to examine the test-retest reliability, and convergent and discriminative validity of a new taekwondo-specific change-of-direction (COD) speed test with striking techniques (TST) in elite taekwondo athletes. Twenty (10 males and 10 females) elite (athletes who compete at national level) and top-elite (athletes who compete at national and international level) taekwondo athletes with an average training background of 8.9 ± 1.3 years of systematic taekwondo training participated in this study. During the two-week test-retest period, various generic performance tests measuring COD speed, balance, speed, and jump performance were carried out during the first week and as a retest during the second week. Three TST trials were conducted with each athlete and the best trial was used for further analyses. The relevant performance measure derived from the TST was the time with striking penalty (TST-TSP). TST-TSP performances amounted to 10.57 ± 1.08 s for males and 11.74 ± 1.34 s for females. The reliability analysis of the TST performance was conducted after logarithmic transformation, in order to address the problem of heteroscedasticity. In both groups, the TST demonstrated a high relative test-retest reliability (intraclass correlation coefficients and 90\% compatibility limits were 0.80 and 0.47 to 0.93, respectively). For absolute reliability, the TST's typical error of measurement (TEM), 90\% compatibility limits, and magnitudes were 4.6\%, 3.4 to 7.7, for males, and 5.4\%, 3.9 to 9.0, for females. The homogeneous sample of taekwondo athletes meant that the TST's TEM exceeded the usual smallest important change (SIC) with 0.2 effect size in the two groups. The new test showed mostly very large correlations with linear sprint speed (r = 0.71 to 0.85) and dynamic balance (r = -0.71 and -0.74), large correlations with COD speed (r = 0.57 to 0.60) and vertical jump performance (r = -0.50 to -0.65), and moderate correlations with horizontal jump performance (r = -0.34 to -0.45) and static balance (r = -0.39 to -0.44). Top-elite athletes showed better TST performances than elite counterparts. Receiver operating characteristic analysis indicated that the TST effectively discriminated between top-elite and elite taekwondo athletes. In conclusion, the TST is a valid, and sensitive test to evaluate the COD speed with taekwondo specific skills, and reliable when considering ICC and TEM. Although the usefulness of the TST is questioned to detect small performance changes in the present population, the TST can detect moderate changes in taekwondo-specific COD speed.}, language = {en} } @article{DelfanJuybariGorganiFiruzjaeeetal.2022, author = {Delfan, Maryam and Juybari, Raheleh Amadeh and Gorgani-Firuzjaee, Sattar and Nielsen, Jens H{\o}iriis and Delfan, Neda and Laher, Ismail and Saeidi, Ayoub and Granacher, Urs and Zouhal, Hassane}, title = {High-Intensity Interval Training Improves Cardiac Function by miR-206 Dependent HSP60 Induction in Diabetic Rats}, series = {Frontiers in Cardiovascular Medicine}, volume = {9}, journal = {Frontiers in Cardiovascular Medicine}, publisher = {Frontiers}, address = {Lausanne, Schweiz}, issn = {2297-055X}, doi = {10.3389/fcvm.2022.927956}, pages = {1 -- 11}, year = {2022}, abstract = {Objective: A role for microRNAs is implicated in several biological and pathological processes. We investigated the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on molecular markers of diabetic cardiomyopathy in rats. Methods: Eighteen male Wistar rats (260 ± 10 g; aged 8 weeks) with streptozotocin (STZ)-induced type 1 diabetes mellitus (55 mg/kg, IP) were randomly allocated to three groups: control, MICT, and HIIT. The two different training protocols were performed 5 days each week for 5 weeks. Cardiac performance (end-systolic and end-diastolic dimensions, ejection fraction), the expression of miR-206, HSP60, and markers of apoptosis (cleaved PARP and cytochrome C) were determined at the end of the exercise interventions. Results: Both exercise interventions (HIIT and MICT) decreased blood glucose levels and improved cardiac performance, with greater changes in the HIIT group (p < 0.001, η2: 0.909). While the expressions of miR-206 and apoptotic markers decreased in both training protocols (p < 0.001, η2: 0.967), HIIT caused greater reductions in apoptotic markers and produced a 20\% greater reduction in miR-206 compared with the MICT protocol (p < 0.001). Furthermore, both training protocols enhanced the expression of HSP60 (p < 0.001, η2: 0.976), with a nearly 50\% greater increase in the HIIT group compared with MICT. Conclusions: Our results indicate that both exercise protocols, HIIT and MICT, have the potential to reduce diabetic cardiomyopathy by modifying the expression of miR-206 and its downstream targets of apoptosis. It seems however that HIIT is even more effective than MICT to modulate these molecular markers.}, language = {en} } @article{SaidiZouhalBoullosaetal.2022, author = {Saidi, Karim and Zouhal, Hassane and Boullosa, Daniel and Dupont, Gregory and Hackney, Anthony C. and Bideau, Benoit and Granacher, Urs and Ben Abderrahman, Abderraouf}, title = {Biochemical markers and wellness status during a congested match play period in elite soccer players}, series = {International journal of sports physiology and performance : IJSSP}, volume = {17}, journal = {International journal of sports physiology and performance : IJSSP}, number = {4}, publisher = {Human Kinetics Publ.}, address = {Champaign}, issn = {1555-0265}, doi = {10.1123/ijspp.2020-0914}, pages = {605 -- 620}, year = {2022}, abstract = {Objectives: To analyze biochemical markers, wellness status, and physical fitness in elite soccer players in relation to changes in training and match exposure during a congested period of match play. Methods: Fourteen elite soccer players were evaluated 3 times (T1, T2, and T3) over 12 weeks (T1-T2: 6-wk regular period of match play and T2-T3: 6-wk congested period of match play). Players performed vertical jump tests, repeated shuttle sprint ability test, and the Yo-Yo Intermittent Recovery Test at T1, T2, and T3. Plasma C-reactive protein, creatinine, and creatine kinase were analyzed at T1, T2, and T3. Wellness status was measured daily using the Hopper questionnaire (delayed onset of muscle soreness, stress, fatigue, and sleep quality). Training session rating of perceived exertion was also recorded on a daily basis. Results: A significant increase was found in stress, fatigue, delayed onset of muscle soreness scores, and Hopper index during the congested period (between T2 and T3) compared with the regular period (between T1 and T2) (.001 < P < .008, 0.8 < ES < 2.3). Between T2 and T3, significant relationships were found between the percentage variations (Delta\%) of C-reactive protein, and Delta\% of creatine kinase with the Hopper Index, and the Delta\% of fatigue score. In addition, the Delta\% of fatigue score and Delta\% of delayed onset of muscle soreness score correlated with Delta\% Yo-Yo Intermittent Recovery Test and Delta\% best of repeated shuttle sprint ability test (.49 < r < P < .01). Conclusions: An intensive period of congested match play significantly compromised elite soccer players' physical fitness and wellness status. Elite soccer players' wellness status reflects declines in physical fitness during this period while biochemical changes do not.}, language = {en} } @article{DelfanJuybariGorganiFiruzjaeeetal.2022, author = {Delfan, Maryam and Juybari, Raheleh Amadeh and Gorgani-Firuzjaee, Sattar and H{\o}iriis Nielsen, Jens and Delfan, Neda and Laher, Ismail and Saeidi, Ayoub and Granacher, Urs and Zouhal, Hassane}, title = {High-intensity interval training improves cardiac function by miR-206 dependent HSP60 induction in diabetic rats}, series = {Frontiers in cardiovascular medicine}, volume = {9}, journal = {Frontiers in cardiovascular medicine}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {2297-055X}, doi = {10.3389/fcvm.2022.927956}, pages = {11}, year = {2022}, abstract = {ObjectiveA role for microRNAs is implicated in several biological and pathological processes. We investigated the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on molecular markers of diabetic cardiomyopathy in rats. MethodsEighteen male Wistar rats (260 +/- 10 g; aged 8 weeks) with streptozotocin (STZ)-induced type 1 diabetes mellitus (55 mg/kg, IP) were randomly allocated to three groups: control, MICT, and HIIT. The two different training protocols were performed 5 days each week for 5 weeks. Cardiac performance (end-systolic and end-diastolic dimensions, ejection fraction), the expression of miR-206, HSP60, and markers of apoptosis (cleaved PARP and cytochrome C) were determined at the end of the exercise interventions. ResultsBoth exercise interventions (HIIT and MICT) decreased blood glucose levels and improved cardiac performance, with greater changes in the HIIT group (p < 0.001, eta(2): 0.909). While the expressions of miR-206 and apoptotic markers decreased in both training protocols (p < 0.001, eta(2): 0.967), HIIT caused greater reductions in apoptotic markers and produced a 20\% greater reduction in miR-206 compared with the MICT protocol (p < 0.001). Furthermore, both training protocols enhanced the expression of HSP60 (p < 0.001, eta(2): 0.976), with a nearly 50\% greater increase in the HIIT group compared with MICT. ConclusionsOur results indicate that both exercise protocols, HIIT and MICT, have the potential to reduce diabetic cardiomyopathy by modifying the expression of miR-206 and its downstream targets of apoptosis. It seems however that HIIT is even more effective than MICT to modulate these molecular markers.}, language = {en} }