@article{BaesslerWeissWienkoopetal.2009,
  author    = {Baessler, Olivia Y. and Weiss, Julia and Wienkoop, Stefanie and Lehmann, Karola and Scheler, Christian and Doelle, Sabine and Schwarz, Dietmar and Franken, Philipp and George, Eckhard and Worm, Margitta and Weckwerth, Wolfram},
  title     = {Evidence for novel tomato seed allergens : IgE-reactive legumin and vicilin proteins identified by multidimensional protein fractionation-mass spectrometry and in silico epitope modeling},
  issn      = {1535-3893},
  doi       = {10.1021/Pr800186d},
  year      = {2009},
  abstract  = {Tomato fruit and seed allergens were detected by IgE-immunoblotting using sera from 18 adult tomato-sensitized patients selected based on a positive history skin prick test (SPT) and specific Immunglobulin (Ig) E-levels. Isolated tomato seed total protein showed high SPT activity comparable or even higher than tomato fruit protein. For the molecular characterization of tomato seed allergens, a multidimensional protein fractionation strategy and LC-MS/MS was used. Two legumin- and vicilin-proteins were purified and showed strong IgE-reactivity in immunoblots. Individual patient sera exhibited varying IgE-sensitivity against the purified proteins. In silico structural modeling indicates high homology between epitopes of known walnut allergens and the detected IgE-crossreactive tomato proteins.},
  language  = {en}
}