@article{GhindilisMakowerScheller1995, author = {Ghindilis, A. L. and Makower, Alexander and Scheller, Frieder W.}, title = {Laccase - glucose dehydrogenase recycling enzyme electrode based on potentiometric mediatorless electrocatalytic detection}, year = {1995}, language = {en} } @article{GhindilisMakowerScheller1995, author = {Ghindilis, A. L. and Makower, Alexander and Scheller, Frieder W.}, title = {Nanomolar determination of the ferrocene derivatives using a recycling enzyme electrode : development of the redox label immunoassay}, year = {1995}, language = {en} } @article{GhindilisMakowerScheller1995, author = {Ghindilis, A. L. and Makower, Alexander and Scheller, Frieder W.}, title = {Potentiometric enzyme electrodes based on substrate recycling and mediatorless bioelectrocatalysis}, year = {1995}, language = {en} } @article{LisdatWollenbergerPaeschkeetal.1998, author = {Lisdat, Fred and Wollenberger, Ursula and Paeschke, Manfred and Scheller, Frieder W.}, title = {Sensitive catecholamine measurement using a monoenzymatic recycling system}, year = {1998}, language = {en} } @article{StancikMacholanPluhaceketal.1995, author = {Stanc{\´i}k, L. and Machol{\´a}n, L. and Pluhacek, I. and Scheller, Frieder W.}, title = {Biosensing of rapeseed glucosinolates using amperometric enzyme electrodes based on membrane-bound glucose oxidase or tyrosinase}, year = {1995}, language = {en} } @article{EremenkoMakowerJinetal.1995, author = {Eremenko, A. V. and Makower, Alexander and Jin, Wen and R{\"u}ger, P. and Scheller, Frieder W.}, title = {Biosensor based on an enzyme modified electrode for highly - sensitive measurement of polyphenols}, year = {1995}, language = {en} } @article{IlievKaishevaSchelleretal.1995, author = {Iliev, I. and Kaisheva, A. and Scheller, Frieder W. and Pfeiffer, Dorothea}, title = {Amperometric gas-diffusion / enzyme electrode}, year = {1995}, language = {en} } @article{RiedelBeyersdorfRadeckNeumannetal.1995, author = {Riedel, K. and Beyersdorf-Radeck, Baerbel and Neumann, B. and Scheller, Frieder W. and Schmid, Rolf D.}, title = {Microbial sensors for determination of aromatics and their chloro derivatives. Part III: Determination of chlorinated phenols using a biosensor containing Trichosporon beigelii (cutaneum)}, year = {1995}, language = {en} } @article{ShumyantsevaIvanovBistolasetal.2004, author = {Shumyantseva, V. V. and Ivanov, Y. D. and Bistolas, Nikitas and Scheller, Frieder W. and Archakov, Alexander I. and Wollenberger, Ursula}, title = {Direct electron transfer of cytochrome P450 2B4 at electrodes modified with non-ionic detergent and colloidal clay nanoparticles}, year = {2004}, abstract = {A method for construction of biosensors with membranous cytochrome P450 isoenzymes was developed based on clay/ detergent/protein mixed films. Thin films of sodium montmorillonite colloid with incorporated cytochrome P450 2134 (CYP2B4) with nonionic detergent were prepared on glassy carbon electrodes. The modified electrodes were electrochemically characterized, and bio-electrocatalytic reactions were followed. CYP2B4 can be reduced fast on clay- modified glassy carbon electrodes in the presence of the nonionic detergent Tween 80. In anaerobic solutions, reversible oxidation and reduction is obtained with a formal potential between -0.292 and - 0.305 V vs Ag/AgCl 1 M KCl depending on the preparation of the biosensor. In air-saturated solution, bio-electrocatalytic reduction currents can be obtained with the CYP2B4-modified electrode on addition of typical substrates such as aminopyrine and benzphetamine. This reaction was suppressed when methyrapone, an inhibitor of P450 reactions, was present. Measurement of product formation also indicates the bioelectrocatialysis by CYP2B4}, language = {en} } @article{FrascavonGrabergFengetal.2010, author = {Frasca, Stefano and von Graberg, Till and Feng, Jiu-Ju and Thomas, Arne and Smarsly, Bernd M. and Weidinger, Inez M. and Scheller, Frieder W. and Hildebrandt, Peter and Wollenberger, Ursula}, title = {Mesoporous indium tin oxide as a novel platform for bioelectronics}, issn = {1867-3880}, doi = {10.1002/cctc.201000047}, year = {2010}, abstract = {Stable immobilization and reversible electrochemistry of cytochrome c in a tranparent indium tin oxide film with a well-defined mesoporosity (mpITO) is demonstrated. the transparency and good conductivity, in combination with the large surface area of mpITO, allow the incorporation of a high amount of elelctroactive biomolecules and their electrochemical and spectroscopic investigation. UV/Vis and resonance Raman spectroscopy, in combination with direct protein voltammetry are employed for the characterization of cytochrome c immobilized in the mpITO and reveal no perturbant of the structural of the integrity of the redox protein. The potential of this modified material as a biosensor detection of superoxide anions is also demonstrated.}, language = {en} } @article{StoeckleinWarsinkeMicheeletal.1998, author = {St{\"o}cklein, Walter F. M. and Warsinke, Axel and Micheel, Burkhard and H{\"o}hne, Wolfgang and Woller, Jochen and Kempter, Gerhard and Scheller, Frieder W.}, title = {Characterization of a monoclonal antibody and its Fab fragment against diphenylurea hapten with BIA}, isbn = {3-8154-3540-4}, year = {1998}, language = {en} } @article{WarsinkeStancikMacholanetal.1998, author = {Warsinke, Axel and Stancik, L. and Machol{\´a}n, L. and Pfeiffer, Dorothea and Scheller, Frieder W.}, title = {Biosensors for food analysis : application of biosensors to food requirements}, isbn = {0-85404-750-6}, year = {1998}, language = {en} } @article{StoeckleinWarsinkeMicheeletal.1997, author = {St{\"o}cklein, Walter F. M. and Warsinke, Axel and Micheel, Burkhard and H{\"o}hne, Wolfgang and Woller, Jochen and Kempter, Gerhard and Scheller, Frieder W.}, title = {Detection of diphenylurea derivatives with biospecific interaction analysis (BIA) : Kinetic investigations}, year = {1997}, language = {en} } @article{SpricigoDronovLisdatetal.2009, author = {Spricigo, Roberto and Dronov, Roman and Lisdat, Fred and Leimk{\"u}hler, Silke and Scheller, Frieder W. and Wollenberger, Ursula}, title = {Electrocatalytic sulfite biosensor with human sulfite oxidase co-immobilized with cytochrome c in a polyelectrolyte-containing multilayer}, issn = {1618-2642}, doi = {10.1007/s00216-008-2432-y}, year = {2009}, abstract = {An efficient electrocatalytic biosensor for sulfite detection was developed by co-immobilizing sulfite oxidase and cytochrome c with polyaniline sulfonic acid in a layer-by-layer assembly. QCM, UV-Vis spectroscopy and cyclic voltammetry revealed increasing loading of electrochemically active protein with the formation of multilayers. The sensor operates reagentless at low working potential. A catalytic oxidation current was detected in the presence of sulfite at the modified gold electrode, polarized at +0.1 V ( vs. Ag/AgCl 1 M KCl). The stability of the biosensor performance was characterized and optimized. A 17-bilayer electrode has a linear range between 1 and 60 mu M sulfite with a sensitivity of 2.19 mA M-1 sulfite and a response time of 2 min. The electrode retained a stable response for 3 days with a serial reproducibility of 3.8\% and lost 20\% of sensitivity after 5 days of operation. It is possible to store the sensor in a dry state for more than 2 months. The multilayer electrode was used for determination of sulfite in unspiked and spiked samples of red and white wine. The recovery and the specificity of the signals were evaluated for each sample.}, language = {en} } @article{EhrentreichFoersterShishniashviliSongetal.1998, author = {Ehrentreich-F{\"o}rster, Eva and Shishniashvili, D. and Song, Min Ik and Scheller, Frieder W.}, title = {Study of antioxidative substances by means of a ssuperoxide sensor}, year = {1998}, language = {en} } @article{KulysDrungilieneWollenbergeretal.1998, author = {Kulys, J. and Drungiliene, A. and Wollenberger, Ursula and Scheller, Frieder W.}, title = {Membrane covered carbon paste electrode for the electrochemical determination of perioxidase and microperoxidase in a flow system}, year = {1998}, language = {en} } @article{JinBernhardtStoeckleinetal.1998, author = {Jin, Wen and Bernhardt, Rita and St{\"o}cklein, Walter F. M. and Scheller, Frieder W.}, title = {Direct electron transfer of adrenodoxin-a [2Fe-2S] protein-- and its mutants on modified gold electrode}, year = {1998}, language = {en} } @article{WollenbergerNeumannScheller1998, author = {Wollenberger, Ursula and Neumann, B. and Scheller, Frieder W.}, title = {Development of a biomimetic alkane sensor f}, year = {1998}, language = {en} } @article{Scheller1998, author = {Scheller, Frieder W.}, title = {Biosensorik}, year = {1998}, language = {de} } @article{SchellerPfeifferLisdatetal.1998, author = {Scheller, Frieder W. and Pfeiffer, Dorothea and Lisdat, Fred and Bauer, Christian G. and Gajovic, Nenad}, title = {Enzyme biosensors based on oxygen detection}, year = {1998}, language = {en} } @article{BogdanovskayaFridmanTarasevichetal.1994, author = {Bogdanovskaya, V. A. and Fridman, Vadim and Tarasevich, M. R. and Scheller, Frieder W.}, title = {Bioelectrocatalysis by immobilized peroxidase : the reaction mechanism and the possibility of electroanalytical detection of both inhibitors and activators of enzyme}, year = {1994}, language = {en} } @article{KirsteinKirsteinSchelleretal.1994, author = {Kirstein, Dieter and Kirstein, Lincoln and Scheller, Frieder W. and Dieckmann, St. and Ronnenberg, J. and Beckmann, Dieter and Weckenbrock, E.}, title = {Elektroenzymatische Reduktion von Nitrat}, year = {1994}, language = {de} } @article{WollenbergerNeumannRiedeletal.1994, author = {Wollenberger, Ursula and Neumann, B. and Riedel, K. and Scheller, Frieder W.}, title = {Enzyme and microbial sensors for phosphate, phenols, pesticides and peroxides}, year = {1994}, language = {en} } @article{YarmanScheller2013, author = {Yarman, Aysu and Scheller, Frieder W.}, title = {Coupling biocatalysis with molecular imprinting in a biomimetic sensor}, series = {Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition}, volume = {52}, journal = {Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition}, number = {44}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1433-7851}, doi = {10.1002/anie.201305368}, pages = {11521 -- 11525}, year = {2013}, language = {en} } @article{SchellerPfeiffer1997, author = {Scheller, Frieder W. and Pfeiffer, Dorothea}, title = {Commercial devices based on amperometric biosensors}, year = {1997}, language = {en} } @article{SchulmeisterRoseScheller1997, author = {Schulmeister, Thomas and Rose, J{\"u}rgen and Scheller, Frieder W.}, title = {Mathematical modelling of exponential amplification in membrane-based enzyme sensors}, year = {1997}, language = {en} } @article{WarsinkeBenkertScheller1996, author = {Warsinke, Axel and Benkert, Alexander and Scheller, Frieder W.}, title = {Biomolecular modules for creatinine determination}, year = {1996}, language = {en} } @article{StoeckleinScheller1996, author = {St{\"o}cklein, Walter F. M. and Scheller, Frieder W.}, title = {Laccase : a marker enzyme for solvent modified immunoassays}, year = {1996}, language = {en} } @article{HalamekWollenbergerStoeckleinetal.2007, author = {Hal{\´a}mek, Jan and Wollenberger, Ursula and St{\"o}cklein, Walter F. M. and Scheller, Frieder W.}, title = {Development of a biosensor for glycated hemoglobin}, issn = {0013-4686}, doi = {10.1016/j.electacta.2007.03.059}, year = {2007}, abstract = {The development of an electrochemical piezoelectric sensor for the detection of glycated hemoglobin is presented. The total hemoglobin (Hb) content is monitored with a mass-sensitive quartz crystal modified with surfactants, and the glycated fraction of the immobilized Hb is determined by subsequent voltarnmetric measurement of the coupled ferroceneboronic acid. Different modifications of the sensor were tested for their hemoglobin binding ability. Deoxycholate (DOCA) was found to be the most suitable among the examined modifiers. Piezoelectric quartz crystals with gold electrodes were modified with DOCA by covalent binding to a pre-formatted 4-aminothiophenol monolayer. The properties of the Hb binding to DOCA and the pH effect on this interaction were studied. In the proposed assay for glycated hemoglobin at first an Hb sample is incubated with ferroceneboronic acid (FcBA), which binds to the fructosyl residue of the glycated Hb. Then this preincubated Hb sample is allowed to interact with the DOCA-modified piezoelectric quartz crystal. The binding is monitored by quartz crystal nanobalance QCN). The amount of FcBA present on the sensor surface is determined by square wave voltammetry. The binding of FcBA results in well-defined peaks with an EO' of +200 mV versus Ag/AgC1 (1 M KC1). The peak height depends on the degree of glycated Hb in the sample ranging from 0\% to 20\% of total Hb. The regeneration of the sensing surface is achieved by pepsin digestion of the deposited Hb. Thus the sensor can be re-used more than 30 times.}, language = {en} } @article{HalamekWollenbergerStoeckleinetal.2007, author = {Hal{\´a}mek, Jan and Wollenberger, Ursula and St{\"o}cklein, Walter F. M. and Warsinke, Axel and Scheller, Frieder W.}, title = {Signal amplification in immunoassays using labeling via boronic acid binding to the sugar moiety of immunoglobulin G : proof of concept for glycated hemoglobin}, issn = {0003-2719}, doi = {10.1080/00032710701327096}, year = {2007}, abstract = {A novel electrochemical immunoassay based on the multiple affinity labeling of the indicator antibody with an electro-active tag is presented. The concept is illustrated for the determination of the glycated hemoglobin HbA1c in hemoglobin samples. Hemoglobin is adsorbed to the surfactant-modified surface of a piezoelectric quartz crystal. Whereas the quartz crystal nanobalance is used to validate the total Hb binding, the HbA1c on the sensor surface is recognized by an antibody and quantified electrochemically after the sugar moieties of the antibody have been labeled in-situ with ferroceneboronic acid. The sensitivity of this sensor is about threefold higher than the sensitivity of a hemoglobin sensor, where the ferroceneboronic acid is bound directly to HbA1c.}, language = {en} } @article{Scheller2009, author = {Scheller, Frieder W.}, title = {Tribute to Guenter Gauglitz (Editorial)}, issn = {1618-2642}, doi = {10.1007/s00216-008-2548-0}, year = {2009}, language = {en} } @article{YarmanWollenbergerScheller2013, author = {Yarman, Aysu and Wollenberger, Ursula and Scheller, Frieder W.}, title = {Sensors based on cytochrome P450 and CYP mimicking systems}, series = {ELECTROCHIMICA ACTA}, volume = {110}, journal = {ELECTROCHIMICA ACTA}, publisher = {PERGAMON-ELSEVIER SCIENCE LTD}, address = {OXFORD}, issn = {0013-4686}, doi = {10.1016/j.electacta.2013.03.154}, pages = {63 -- 72}, year = {2013}, abstract = {Cytochrome P450 enzymes (CYPs) act on more than 90 percent of all drugs currently on the market. The catalytic cycle requires electron supply to the heme iron in the presence of oxygen. Electrochemistry allows to characterise the reaction mechanism of these redox enzymes by observing the electron transfer in real time. According to the number of publications on protein electrochemistry CYP has the third position after glucose oxidase and cytochrome c. CYP based enzyme electrodes for the quantification of drugs, metabolites or pesticides have been developed using different iso-enzymes. A crucial step in the sensor development is the efficiency of coupling the biocatalytic systems with the electrode is. In the 1970s the direct electron transfer of heme and heme peptides called microperoxidases (MPs) was used as model of oxidoreductases. They exhibit a broad substrate spectrum including hydroxylation of selected aromatic substrates, demethylation and epoxidation by means of hydrogen peroxide. It overlaps with that of P450 making heme and MPs to alternate recognition elements in biosensors for the detection of typical CYP substrates. In these enzyme electrodes the signal is generated by the conversion of all substrates thus representing in complex media an overall parameter. By combining the biocatalytic substrate conversion with selective binding to a molecularly imprinted polymer layer the specificity has been improved. Here we discuss different approaches of biosensors based on CYP, microperoxidases and catalytically active MIPs and discuss their potential as recognition elements in biosensors. The performance of these sensors and their further development are discussed. (C) 2013 Elsevier Ltd. All rights reserved.}, language = {en} } @article{LehmannWollenbergerBrigeliusFloheetal.1998, author = {Lehmann, Claudia and Wollenberger, Ursula and Brigelius-Floh{\´e}, Regina and Scheller, Frieder W.}, title = {Bioelectrocatalysis by a selenoenzyme}, year = {1998}, language = {en} } @article{KirsteinKirsteinSchelleretal.1998, author = {Kirstein, Dieter and Kirstein, Lincoln and Scheller, Frieder W. and Borcherding, H.}, title = {Amperometric nitrate biosensors on the basis of Pseudomonas stutzeri nitrate reductase}, year = {1998}, language = {en} } @article{Scheller1998, author = {Scheller, Frieder W.}, title = {Neue Dimensionen der Biosensorik}, year = {1998}, language = {de} } @article{JinWollenbergerScheller1998, author = {Jin, Wen and Wollenberger, Ursula and Scheller, Frieder W.}, title = {PQQ as redox shuttle for quinoprotein glucose dehydrogenase}, year = {1998}, language = {en} } @article{StoeckleinWarsinkeMicheeletal.1998, author = {St{\"o}cklein, Walter F. M. and Warsinke, Axel and Micheel, Burkhard and Kempter, Gerhard and H{\"o}hne, Wolfgang and Scheller, Frieder W.}, title = {Diphenylurea hapten sensing with a monoclonal antibody and its Fab fragment : kinetic and thermodynamic investigations}, year = {1998}, language = {en} } @article{EremenkoBauerMakoweretal.1998, author = {Eremenko, Arkadi V. and Bauer, Christian G. and Makower, Alexander and Kanne, Beate and Baumgarten, Horst and Scheller, Frieder W.}, title = {The development of a non-competitive immunoenzymometric Assay (IEMA) of cocaine}, year = {1998}, language = {en} } @article{StrefferKaatzBaueretal.1998, author = {Streffer, Katrin and Kaatz, Helvi and Bauer, Christian G. and Makower, Alexander and Schulmeister, Thomas and Scheller, Frieder W. and Peter, Martin G. and Wollenberger, Ursula}, title = {Application of a sensitive catechol detector for determination of tyrosinase inhibitors}, year = {1998}, language = {en} } @article{HuangWarsinkeKuwanaetal.1998, author = {Huang, T. and Warsinke, Axel and Kuwana, T. and Scheller, Frieder W.}, title = {The determination of L-phenylalanine based on a novel NADH-detecting biosensor}, year = {1998}, language = {en} } @article{BauerEremenkoKuehnetal.1998, author = {Bauer, Christian G. and Eremenko, A. V. and K{\"u}hn, A. and K{\"u}rzinger, K. and Markower, Alexander and Scheller, Frieder W.}, title = {Automated amplifield flow immunoassay for cocaine}, year = {1998}, language = {en} } @article{WollenbergerJinBernhardtetal.1998, author = {Wollenberger, Ursula and Jin, Wen and Bernhardt, Rita and Lehmann, Claudia and St{\"o}cklein, Walter F. M. and Brigelius-Floh{\´e}, Regina and Scheller, Frieder W.}, title = {Funktionalisierung von Elektroden f{\"u}r den direkten heterogenen Elektrotransfer}, year = {1998}, language = {de} } @article{GajovicWarsinkeScheller1998, author = {Gajovic, Nenad and Warsinke, Axel and Scheller, Frieder W.}, title = {A bienzyme electrode for L-malate based on a novel and general design}, year = {1998}, language = {en} } @article{LisdatHoWollenbergeretal.1998, author = {Lisdat, Fred and Ho, Wah O. and Wollenberger, Ursula and Scheller, Frieder W. and Richter, Torsten and Bilitewski, Ursula}, title = {Recycling systems based on screen-printed electrodes}, year = {1998}, language = {en} } @article{BaeumnerGauglitzScheller2010, author = {Baeumner, Antje J. and Gauglitz, Guenter and Scheller, Frieder W.}, title = {Focus on bioanalysis}, issn = {1618-2642}, doi = {10.1007/s00216-010-4203-9}, year = {2010}, abstract = {Editoria}, language = {en} } @article{BosserdtGajovicEichelmanScheller2013, author = {Bosserdt, Maria and Gajovic-Eichelman, Nenad and Scheller, Frieder W.}, title = {Modulation of direct electron transfer of cytochrome c by use of a molecularly imprinted thin film}, series = {Analytical \& bioanalytical chemistry}, volume = {405}, journal = {Analytical \& bioanalytical chemistry}, number = {20}, publisher = {Springer}, address = {Heidelberg}, issn = {1618-2642}, doi = {10.1007/s00216-013-7009-8}, pages = {6437 -- 6444}, year = {2013}, abstract = {We describe the preparation of a molecularly imprinted polymer film (MIP) on top of a self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA) on gold, where the template cytochrome c (cyt c) participates in direct electron transfer (DET) with the underlying electrode. To enable DET, a non-conductive polymer film is electrodeposited from an aqueous solution of scopoletin and cyt c on to the surface of a gold electrode previously modified with MUA. The electroactive surface concentration of cyt c was 0.5 pmol cm(-2). In the absence of the MUA layer, no cyt c DET was observed and the pseudo-peroxidatic activity of the scopoletin-entrapped protein, assessed via oxidation of Ampliflu red in the presence of hydrogen peroxide, was only 30 \% of that for the MIP on MUA. This result indicates that electrostatic adsorption of cyt c by the MUA-SAM substantially increases the surface concentration of cyt c during the electrodeposition step, and is a prerequisite for the productive orientation required for DET. After template removal by treatment with sulfuric acid, rebinding of cyt c to the MUA-MIP-modified electrode occurred with an affinity constant of 100,000 mol(-1) L, a value three times higher than that determined by use of fluorescence titration for the interaction between scopoletin and cyt c in solution. The DET of cyt c in the presence of myoglobin, lysozyme, and bovine serum albumin (BSA) reveals that the MIP layer suppresses the effect of competing proteins.}, language = {en} } @article{Scheller1996, author = {Scheller, Frieder W.}, title = {New recognition elements for bioanalytics}, year = {1996}, language = {en} } @article{PfeifferSchubertWollenbergeretal.1996, author = {Pfeiffer, Dorothea and Schubert, Frank and Wollenberger, Ursula and Scheller, Frieder W.}, title = {Electrochemical sensors : enzyme electrodes and field effect transistors}, year = {1996}, language = {en} } @article{WollenbergerScheller1993, author = {Wollenberger, Ursula and Scheller, Frieder W.}, title = {Enzyme activation for activator and enzyme activity measurement}, year = {1993}, language = {en} } @article{WollenbergerNeumannScheller1993, author = {Wollenberger, Ursula and Neumann, B. and Scheller, Frieder W.}, title = {Enzyme and microbial sensors for environmental Monitoring}, year = {1993}, language = {en} }