@article{DraudeKoersgenPelsteretal.2015,
  author    = {Draude, Felix and K{\"o}rsgen, Martin and Pelster, Andreas and Schwerdtle, Tanja and M{\"u}thing, Johannes and Arlinghaus, Heinrich F.},
  title     = {Characterization of freeze-fractured epithelial plasma membranes on nanometer scale with ToF-SIMS},
  series = {Analytical \& bioanalytical chemistry},
  volume    = {407},
  journal   = {Analytical \& bioanalytical chemistry},
  number    = {8},
  publisher = {Springer},
  address   = {Heidelberg},
  issn      = {1618-2642},
  doi       = {10.1007/s00216-014-8334-2},
  pages     = {2203 -- 2211},
  year      = {2015},
  abstract  = {Time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to characterize the freeze-fracturing process of human epithelial PANC-1 and UROtsa cells. For this purpose, phosphatidylcholine, sphingomyelin, phosphatidylethanolamine, and phosphatidylserine standard samples were investigated to find specific signals with both high specificity and signal intensity. The results were used to investigate single cells of subconfluent cell layers prepared with a special silicon wafer sandwich preparation technique. This freeze-fracturing technique strips cell membranes off the cells, isolating them on opposing silicon wafer substrates. Criteria were found for defining regions with stripped off cell membranes and, on the opposing wafer, complementary regions with the remaining cells. Measured ethanolamine/choline and serine/choline ratios in these regions clearly showed that in the freeze-fracturing process, the lipid bilayer of the plasma membrane is split along its central zone. Accordingly, only the outer lipid monolayer is stripped off the cell, while the inner lipid monolayer remains attached to the cell on the opposing wafer, thus allowing detailed analysis of a single lipid monolayer. Furthermore, it could be shown that using different washing procedures did not influence the transmembrane lipid distribution. Under optimized preparation conditions, it became feasible to detect lipids with a lateral resolution of approximately 100 nm. The data indicate that ToF-SIMS would be a very useful technique to study with very high lateral resolution changes in lipid composition caused, for example, by lipid storage diseases or pharmaceuticals that interfere with the lipid metabolism.},
  language  = {en}
}
@inproceedings{DeselOpelSiegerisetal.2023,
  author    = {Desel, J{\"o}rg and Opel, Simone and Siegeris, Juliane and Draude, Claude and Weber, Gerhard and Schell, Timon and Schwill, Andreas and Thorbr{\"u}gge, Carsten and Sch{\"a}fer, Len Ole and Netzer, Cajus Marian and Gerstenberger, Dietrich and Winkelnkemper, Felix and Schulte, Carsten and B{\"o}ttcher, Axel and Thurner, Veronika and H{\"a}fner, Tanja and Ottinger, Sarah and Große-B{\"o}lting, Gregor and Scheppach, Lukas and M{\"u}hling, Andreas and Baberowski, David and Leonhardt, Thiemo and Rentsch, Susanne and Bergner, Nadine and Bonorden, Leif and Stemme, Jonas and Hoppe, Uwe and Weicker, Karsten and Bender, Esther and Barbas, Helena and Hamann, Fabian and Soll, Marcus and Sitzmann, Daniel},
  title     = {Hochschuldidaktik Informatik HDI 2021},
  series = {Commentarii informaticae didacticae},
  booktitle = {Commentarii informaticae didacticae},
  number    = {13},
  editor    = {Desel, J{\"o}rg and Opel, Simone and Siegeris, Juliane},
  publisher = {Universit{\"a}tsverlag Potsdam},
  address   = {Potsdam},
  isbn      = {978-3-86956-548-4},
  issn      = {1868-0844},
  doi       = {10.25932/publishup-56507},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-565070},
  pages     = {299},
  year      = {2023},
  abstract  = {Die Fachtagungen HDI (Hochschuldidaktik Informatik) besch{\"a}ftigen sich mit den unterschiedlichen Aspekten informatischer Bildung im Hochschulbereich. Neben den allgemeinen Themen wie verschiedenen Lehr- und Lernformen, dem Einsatz von Informatiksystemen in der Hochschullehre oder Fragen der Gewinnung von geeigneten Studierenden, deren Kompetenzerwerb oder auch der Betreuung der Studierenden widmet sich die HDI immer auch einem Schwerpunktthema. Im Jahr 2021 war dies die Ber{\"u}cksichtigung von Diversit{\"a}t in der Lehre. Diskutiert wurden beispielsweise die Einbeziehung von besonderen fachlichen und {\"u}berfachlichen Kompetenzen Studierender, der Unterst{\"u}tzung von Durchl{\"a}ssigkeit aus nichtakademischen Berufen, aber auch die Gestaltung inklusiver Lehr- und Lernszenarios, Aspekte des Lebenslangen Lernens oder sich an die Diversit{\"a}t von Studierenden adaptierte oder adaptierende Lehrsysteme. Dieser Band enth{\"a}lt ausgew{\"a}hlte Beitr{\"a}ge der 9. Fachtagung 2021, die in besonderer Weise die Konferenz und die dort diskutierten Themen repr{\"a}sentieren.},
  language  = {de}
}
@article{HaaseArlinghausTentschertetal.2011,
  author    = {Haase, Andrea and Arlinghaus, Heinrich F. and Tentschert, Jutta and Jungnickel, Harald and Graf, Philipp and Mantion, Alexandre and Draude, Felix and Galla, Sebastian and Plendl, Johanna and Goetz, Mario E. and Masic, Admir and Meier, Wolfgang P. and Thuenemann, Andreas F. and Taubert, Andreas and Luch, Andreas},
  title     = {Application of Laser Postionization Secondary Neutral Mass Spectrometry/Time-of-Flight Secondary Ion Mass Spectrometry in Nanotoxicology: Visualization of Nanosilver in Human Macrophages and Cellular Responses},
  series = {ACS nano},
  volume    = {5},
  journal   = {ACS nano},
  number    = {4},
  publisher = {American Chemical Society},
  address   = {Washington},
  issn      = {1936-0851},
  doi       = {10.1021/nn200163w},
  pages     = {3059 -- 3068},
  year      = {2011},
  abstract  = {Silver nanoparticles (SNP) are the subject of worldwide commercialization because of their antimicrobial effects. Yet only little data on their mode of action exist. Further, only few techniques allow for visualization and quantification of unlabeled nanoparticles inside cells. To study SNP of different sizes and coatings within human macrophages, we introduce a novel laser postionization secondary neutral mass spectrometry (Laser-SNMS) approach and prove this method superior to the widely applied confocal Raman and transmission electron microscopy. With time-of-flight secondary ion mass spectrometry (TOF-SIMS) we further demonstrate characteristic fingerprints in the lipid pattern of the cellular membrane indicative of oxidative stress and membrane fluidity changes. Increases of protein carbonyl and heme oxygenase-1 levels in treated cells confirm the presence of oxidative stress biochemically. Intriguingly, affected phagocytosis reveals as highly sensitive end point of SNP-mediated adversity In macrophages. The cellular responses monitored are. hierarchically linked, but follow individual kinetics and are partially reversible.},
  language  = {en}
}