@article{MeyerLopezSerranoMitzeetal.2017,
  author    = {Meyer, S{\"o}ren and Lopez-Serrano, Aniceto and Mitze, Hanna and Jakubowski, Norbert and Schwerdtle, Tanja},
  title     = {Single-cell analysis by ICP-MS/MS as a fast tool for cellular bioavailability studies of arsenite},
  series = {Metallomics : integrated biometal science},
  volume    = {10},
  journal   = {Metallomics : integrated biometal science},
  number    = {1},
  publisher = {Royal Society of Chemistry},
  address   = {Cambridge},
  issn      = {1756-5901},
  doi       = {10.1039/c7mt00285h},
  pages     = {73 -- 76},
  year      = {2017},
  abstract  = {Single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS) has become a powerful and fast tool to evaluate the elemental composition at a single-cell level. In this study, the cellular bioavailability of arsenite (incubation of 25 and 50 mu M for 0-48 h) has been successfully assessed by SC-ICP-MS/MS for the first time directly after re-suspending the cells in water. This procedure avoids the normally arising cell membrane permeabilization caused by cell fixation methods (e.g. methanol fixation). The reliability and feasibility of this SC-ICP-MS/MS approach with a limit of detection of 0.35 fg per cell was validated by conventional bulk ICP-MS/MS analysis after cell digestion and parallel measurement of sulfur and phosphorus.},
  language  = {en}
}